Rmu_sc0000745.1_g000038

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000745.1
OG0003034
Physical Location & Seq
Reverse (-)
139841 .. 140614
774 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
atgatggagccggccgggtcctgcgactactctgatggagcctggatcctcgacccgaatgcgtctctggggatctcccgccgttcgatcccgctagggtttattggtgattccttgaatcggaatatgtttgttgcgcttttctgttcgctgaaacgcgtgtcgagtgaagcaaagaagtggcggccagttggtgctgatcgcgtgttcacatttcttcagtacaatcttgcgtatcatcggacaaatcttttggctcgttatggtaggtggtcggccaattctaatggtggtgtgctcgaatctcttggatataaagagggttatagagttgatgttgacattccggtagacacatgggcagaatctctaagcgttcatgacattctgatattcaacacagggcactggtaa

Protein Analysis

137

Amino Acids

15.39

Weight (kDa)

6.82

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 31 - 137 1e-14 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 159, 204
AciI CCGC 3 cut(s) 79, 92, 184
AclWI GGATC 4 cut(s) 40, 53, 80, 82
AcoI YGGCCR 3 cut(s) 12, 185, 276
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 120
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 2 cut(s) 118, 397
AjnI CCWGG 1 cut(s) 41
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 4 cut(s) 40, 53, 80, 82
AoxI GGCC 3 cut(s) 12, 185, 276
ArsI GACNNNNNNTTYG 2 cut(s) 235, 267
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 408
BamHI GGATCC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 300
BccI CCATC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 95
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
Bme1390I CCNGG 2 cut(s) 16, 43
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 4 cut(s) 9, 19, 40, 47
BmrFI CCNGG 2 cut(s) 16, 43
BpuMI CCSGG 1 cut(s) 16
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 2 cut(s) 188, 413
BseBI CCWGG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 120
BseNI ACTGG 2 cut(s) 188, 413
BseSI GKGCMC 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 12
Bsh1236I CGCG 2 cut(s) 159, 204
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 3 cut(s) 14, 187, 278
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 3 cut(s) 11, 15, 347
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 1 cut(s) 69
BsmBI CGTCTC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 3 cut(s) 14, 187, 278
Bsp1286I GDGCHC 2 cut(s) 300, 408
Bsp143I GATC 4 cut(s) 45, 72, 87, 199
BspACI CCGC 3 cut(s) 79, 92, 184
BspANI GGCC 3 cut(s) 14, 187, 278
BspFNI CGCG 2 cut(s) 159, 204
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 4 cut(s) 9, 19, 40, 47
BspPI GGATC 4 cut(s) 40, 53, 80, 82
BsrFI RCCGGY 1 cut(s) 10
BsrI ACTGG 2 cut(s) 188, 413
BssAI RCCGGY 1 cut(s) 10
BssMI GATC 4 cut(s) 45, 72, 87, 199
Bst2UI CCWGG 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 371
BstFNI CGCG 2 cut(s) 159, 204
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 4 cut(s) 48, 75, 90, 202
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 4 cut(s) 45, 72, 87, 199
BstMCI CGRYCG 1 cut(s) 15
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 14, 41
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 2 cut(s) 159, 204
BstX2I RGATCY 2 cut(s) 45, 72
BstYI RGATCY 2 cut(s) 45, 72
BstZI CGGCCG 1 cut(s) 12
BsuRI GGCC 3 cut(s) 14, 187, 278
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 12
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 18
CseI GACGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 357, 380
CviJI RGCY 6 cut(s) 10, 14, 41, 187, 257, 278
CviKI_1 RGCY 6 cut(s) 10, 14, 41, 187, 257, 278
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 371
DpnI GATC 4 cut(s) 47, 74, 89, 201
DpnII GATC 4 cut(s) 45, 72, 87, 199
EaeI YGGCCR 3 cut(s) 12, 185, 276
EagI CGGCCG 1 cut(s) 12
EclXI CGGCCG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 18
Eco52I CGGCCG 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 2 cut(s) 360, 383
FaiI YATR 6 cut(s) 128, 264, 315, 327, 358, 381
FatI CATG 2 cut(s) 356, 379
FauI CCCGC 2 cut(s) 86, 99
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 3 cut(s) 14, 187, 278
HapII CCGG 3 cut(s) 11, 15, 347
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 139
Hin1II CATG 2 cut(s) 360, 383
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 4 cut(s) 110, 118, 302, 365
HpaII CCGG 3 cut(s) 11, 15, 347
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 3 cut(s) 210, 340, 352
Hpy188I TCNGA 4 cut(s) 34, 123, 243, 390
Hpy188III TCNNGA 1 cut(s) 380
Hpy8I GTNNAC 3 cut(s) 210, 340, 352
HpyF3I CTNAG 1 cut(s) 371
Hsp92II CATG 2 cut(s) 360, 383
HspAI GCGC 1 cut(s) 137
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Kzo9I GATC 4 cut(s) 45, 72, 87, 199
LmnI GCTCC 2 cut(s) 7, 38
MaeI CTAG 1 cut(s) 95
MalI GATC 4 cut(s) 47, 74, 89, 201
MboI GATC 4 cut(s) 45, 72, 87, 199
MboII GAAGA 1 cut(s) 209
MflI RGATCY 2 cut(s) 45, 72
MhlI GDGCHC 2 cut(s) 300, 408
MluCI AATT 1 cut(s) 280
MluI ACGCGT 1 cut(s) 157
MnlI CCTC 2 cut(s) 59, 313
MroNI GCCGGC 1 cut(s) 10
MspI CCGG 3 cut(s) 11, 15, 347
MspR9I CCNGG 2 cut(s) 16, 43
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 2 cut(s) 159, 204
NaeI GCCGGC 1 cut(s) 12
NciI CCSGG 1 cut(s) 16
NdeII GATC 4 cut(s) 45, 72, 87, 199
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 2 cut(s) 360, 383
NlaIV GGNNCC 4 cut(s) 9, 19, 40, 47
PagI TCATGA 1 cut(s) 379
PctI GAATGC 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 12
PfeI GAWTC 4 cut(s) 110, 118, 302, 365
PkrI GCNGC 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 4 cut(s) 9, 19, 40, 47
PspPI GGNCC 1 cut(s) 18
PspPPI RGGWCCY 1 cut(s) 18
PsuI RGATCY 2 cut(s) 45, 72
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 4 cut(s) 45, 72, 87, 199
Sau96I GGNCC 1 cut(s) 18
ScrFI CCNGG 2 cut(s) 16, 43
SduI GDGCHC 2 cut(s) 300, 408
SetI ASST 1 cut(s) 272
SinI GGWCC 1 cut(s) 18
Sse9I AATT 1 cut(s) 280
SsiI CCGC 3 cut(s) 79, 92, 184
SspMI CTAG 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 14, 41
TaqI TCGA 4 cut(s) 51, 86, 164, 300
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 4 cut(s) 110, 118, 302, 365
TscAI CASTG 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 368
TspRI CASTG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 18
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.