Rmu_sc0001049.1_g000002

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001049.1
OG0003959
Physical Location & Seq
Forward (+)
3164 .. 4411
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
atgaccaaggtgtacggaacaggcgcgtacgatttcaagcgccaccatgttgcagagtacccggtggagctgaaccaccagctcggagagaagccggtggaggctaaacccggtgcggcgctgccgagctcgataacgctgtcggagattcagagggaccagctcactatgattgccgctgcgaattggtccaaagctggcggcgacgccgcgcaggtgaagaaggcgttcaacacggagctggtgaagaagatttacgagacggagttgtgtgtgaaagaggggcagaggaagacggtgccattgcagagagtgatgattttggaggtcagccagtatttggagaaccatatctttcccaatttcgatcccaaaacggcgacgttcgaacacgttatgtccatgattctcatggtcaatgagaaggtaatgccttttggaattgcagtatttactttgggtcacattagggaggcgttgtaccttgcttgtgccggccccattttggtgattgatcaccaagagctgggtggaaaaggtgtccaagatgtcatccatgtcaaaagccaagtgtttgagctcatccagccacagcttcaccgccttgcttgtgagagttgcttcttctcggcatcctgcagcacagctccaattgcagacagagttgcactccatttcttggaaatgaccgaagctgagcaagttgtgggggggtcaactcatcaagcgcaactgaagaaaatcccaaagaaaaacacctccaccaccatttctgctgtcgacgacttcggattcaataaggcttccaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

270

Amino Acids

29.84

Weight (kDa)

6.83

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 55 - 143 6.5e-19 Intron-binding protein aquarius N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 205
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 298
AccB7I CCANNNNNTGG 3 cut(s) 340, 526, 679
AccI GTMKAC 1 cut(s) 780
AccII CGCG 2 cut(s) 26, 212
AciI CCGC 5 cut(s) 116, 177, 201, 210, 601
AclWI GGATC 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 4 cut(s) 14, 29, 59, 482
AfiI CCNNNNNNNGG 4 cut(s) 340, 505, 526, 679
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 3 cut(s) 37, 232, 796
Alw21I GWGCWC 2 cut(s) 131, 582
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 1 cut(s) 362
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 3 cut(s) 121, 179, 639
Asp700I GAANNNNTTC 1 cut(s) 227
AspLEI GCGC 5 cut(s) 26, 42, 121, 214, 730
AspS9I GGNCC 3 cut(s) 157, 189, 497
AsuC2I CCSGG 2 cut(s) 62, 111
AsuHPI GGTGA 5 cut(s) 229, 256, 509, 520, 590
AsuII TTCGAA 1 cut(s) 387
AvaII GGWCC 2 cut(s) 157, 189
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 131, 582
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 131, 582
BbvI GCAGC 3 cut(s) 108, 166, 651
BceAI ACGGC 1 cut(s) 393
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 2 cut(s) 62, 111
BcoDI GTCTC 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 637
BfoI RGCGCY 2 cut(s) 43, 122
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 7 cut(s) 117, 122, 177, 180, 202, 210, 640
BlpI GCTNAGC 1 cut(s) 696
BlsI GCNGC 7 cut(s) 118, 123, 178, 181, 203, 211, 641
Bme1390I CCNGG 2 cut(s) 62, 111
Bme18I GGWCC 2 cut(s) 157, 189
BmgT120I GGNCC 3 cut(s) 157, 189, 497
BmiI GGNNCC 3 cut(s) 158, 300, 499
BmrFI CCNGG 2 cut(s) 62, 111
BmsI GCATC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 654, 686
Bpu1102I GCTNAGC 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 62, 111
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 505, 526, 679
Bse118I RCCGGY 2 cut(s) 94, 494
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 302
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 3 cut(s) 552, 582, 632
BseLI CCNNNNNNNGG 4 cut(s) 340, 505, 526, 679
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 1 cut(s) 334
BseXI GCAGC 3 cut(s) 108, 166, 651
BseYI CCCAGC 1 cut(s) 526
Bsh1236I CGCG 2 cut(s) 26, 212
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 1 cut(s) 298
BsiHKAI GWGCWC 2 cut(s) 131, 582
BsiSI CCGG 4 cut(s) 62, 95, 111, 495
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 4 cut(s) 340, 505, 526, 679
BsmAI GTCTC 1 cut(s) 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 131, 582
Bsp143I GATC 2 cut(s) 367, 514
Bsp1720I GCTNAGC 1 cut(s) 696
BspACI CCGC 5 cut(s) 116, 177, 201, 210, 601
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 1 cut(s) 688
BspFNI CGCG 2 cut(s) 26, 212
BspLI GGNNCC 3 cut(s) 158, 300, 499
BspMAI CTGCAG 1 cut(s) 641
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 1 cut(s) 362
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 302
BsrFI RCCGGY 2 cut(s) 94, 494
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 2 cut(s) 94, 494
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 367, 514
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 298
BstACI GRCGYC 1 cut(s) 207
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 199, 496
BstDEI CTNAG 1 cut(s) 696
BstF5I GGATG 3 cut(s) 552, 582, 632
BstFNI CGCG 2 cut(s) 26, 212
BstH2I RGCGCY 2 cut(s) 43, 122
BstHHI GCGC 5 cut(s) 26, 42, 121, 214, 730
BstKTI GATC 2 cut(s) 370, 517
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 2 cut(s) 367, 514
BstMWI GCNNNNNNNGC 2 cut(s) 586, 653
BstSCI CCNGG 2 cut(s) 60, 109
BstSFI CTRYAG 1 cut(s) 637
BstUI CGCG 2 cut(s) 26, 212
BstV1I GCAGC 3 cut(s) 108, 166, 651
BstV2I GAAGAC 1 cut(s) 299
BsuRI GGCC 1 cut(s) 498
BtsCI GGATG 3 cut(s) 552, 582, 632
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 199, 496
CfoI GCGC 5 cut(s) 26, 42, 121, 214, 730
Cfr10I RCCGGY 2 cut(s) 94, 494
Cfr13I GGNCC 3 cut(s) 157, 189, 497
CseI GACGC 1 cut(s) 215
Csp6I GTAC 4 cut(s) 13, 28, 58, 481
CviAII CATG 4 cut(s) 47, 403, 412, 557
CviQI GTAC 4 cut(s) 13, 28, 58, 481
DdeI CTNAG 1 cut(s) 696
DpnI GATC 2 cut(s) 369, 516
DpnII GATC 2 cut(s) 367, 514
Ecl136II GAGCTC 2 cut(s) 129, 580
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 131, 582
Eco47I GGWCC 2 cut(s) 157, 189
Eco53kI GAGCTC 2 cut(s) 129, 580
Eco57I CTGAAG 1 cut(s) 755
EcoICRI GAGCTC 2 cut(s) 129, 580
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 131, 582
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 4 cut(s) 50, 406, 415, 560
FaiI YATR 7 cut(s) 48, 170, 351, 398, 404, 413, 558
FaqI GGGAC 1 cut(s) 170
FatI CATG 4 cut(s) 46, 402, 411, 556
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 7 cut(s) 117, 122, 177, 180, 202, 210, 640
FokI GGATG 3 cut(s) 539, 569, 619
FriOI GRGCYC 2 cut(s) 131, 582
Fsp4HI GCNGC 7 cut(s) 117, 122, 177, 180, 202, 210, 640
GlaI GCGC 5 cut(s) 25, 41, 120, 213, 729
GluI GCNGC 7 cut(s) 117, 122, 177, 180, 202, 210, 640
GsaI CCCAGC 1 cut(s) 530
HaeII RGCGCY 2 cut(s) 43, 122
HaeIII GGCC 1 cut(s) 498
HapII CCGG 4 cut(s) 62, 95, 111, 495
HgaI GACGC 1 cut(s) 215
HhaI GCGC 5 cut(s) 26, 42, 121, 214, 730
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 4 cut(s) 50, 406, 415, 560
Hin6I GCGC 5 cut(s) 24, 40, 119, 212, 728
HinP1I GCGC 5 cut(s) 24, 40, 119, 212, 728
HincII GTYRAC 2 cut(s) 717, 781
HindII GTYRAC 2 cut(s) 717, 781
HinfI GANTC 3 cut(s) 148, 406, 792
HpaII CCGG 4 cut(s) 62, 95, 111, 495
HphI GGTGA 5 cut(s) 229, 256, 509, 520, 590
Hpy166II GTNNAC 3 cut(s) 13, 717, 781
Hpy188I TCNGA 4 cut(s) 86, 145, 153, 791
Hpy8I GTNNAC 3 cut(s) 13, 717, 781
Hpy99I CGWCG 3 cut(s) 209, 385, 785
HpyAV CCTTC 2 cut(s) 217, 418
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 2 cut(s) 383, 393
HpyCH4V TGCA 6 cut(s) 53, 307, 446, 639, 656, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 586, 653
HpyF3I CTNAG 1 cut(s) 696
HpySE526I ACGT 2 cut(s) 383, 393
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 4 cut(s) 50, 406, 415, 560
HspAI GCGC 5 cut(s) 24, 40, 119, 212, 728
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 2 cut(s) 367, 514
LmnI GCTCC 3 cut(s) 67, 238, 652
Lsp1109I GCAGC 3 cut(s) 108, 166, 651
LweI GCATC 1 cut(s) 641
MaeII ACGT 2 cut(s) 383, 393
MaeIII GTNAC 1 cut(s) 461
MalI GATC 2 cut(s) 369, 516
MboI GATC 2 cut(s) 367, 514
MboII GAAGA 6 cut(s) 232, 259, 262, 304, 616, 748
MfeI CAATTG 1 cut(s) 651
MhlI GDGCHC 2 cut(s) 131, 582
MluCI AATT 4 cut(s) 184, 361, 441, 651
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 7 cut(s) 94, 147, 274, 282, 319, 466, 769
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 506
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 4 cut(s) 62, 95, 111, 495
MspR9I CCNGG 2 cut(s) 62, 111
MunI CAATTG 1 cut(s) 651
MvnI CGCG 2 cut(s) 26, 212
MwoI GCNNNNNNNGC 2 cut(s) 586, 653
NaeI GCCGGC 1 cut(s) 496
NciI CCSGG 2 cut(s) 62, 111
NdeII GATC 2 cut(s) 367, 514
NgoMIV GCCGGC 1 cut(s) 494
NlaIII CATG 4 cut(s) 50, 406, 415, 560
NlaIV GGNNCC 3 cut(s) 158, 300, 499
NmeAIII GCCGAG 2 cut(s) 150, 608
NmuCI GTSAC 1 cut(s) 461
NspV TTCGAA 1 cut(s) 387
PaqCI CACCTGC 1 cut(s) 205
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 148, 406, 792
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 3 cut(s) 340, 526, 679
PkrI GCNGC 7 cut(s) 118, 123, 178, 181, 203, 211, 641
Psp124BI GAGCTC 2 cut(s) 131, 582
PspFI CCCAGC 1 cut(s) 526
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 158, 300, 499
PspPI GGNCC 3 cut(s) 157, 189, 497
PstI CTGCAG 1 cut(s) 641
RsaI GTAC 4 cut(s) 14, 29, 59, 482
RsaNI GTAC 4 cut(s) 13, 28, 58, 481
RseI CAYNNNNRTG 1 cut(s) 506
SacI GAGCTC 2 cut(s) 131, 582
SalI GTCGAC 1 cut(s) 779
SatI GCNGC 7 cut(s) 117, 122, 177, 180, 202, 210, 640
Sau3AI GATC 2 cut(s) 367, 514
Sau96I GGNCC 3 cut(s) 157, 189, 497
ScrFI CCNGG 2 cut(s) 62, 111
SduI GDGCHC 2 cut(s) 131, 582
SfaNI GCATC 1 cut(s) 641
SfcI CTRYAG 1 cut(s) 637
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 2 cut(s) 157, 189
SmiMI CAYNNNNRTG 1 cut(s) 506
Sse9I AATT 4 cut(s) 184, 361, 441, 651
SsiI CCGC 5 cut(s) 116, 177, 201, 210, 601
SstI GAGCTC 2 cut(s) 131, 582
StyD4I CCNGG 2 cut(s) 60, 109
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 2 cut(s) 386, 396
TaqI TCGA 4 cut(s) 131, 366, 387, 780
TaqII GACCGA 1 cut(s) 704
TasI AATT 4 cut(s) 184, 361, 441, 651
TauI GCSGC 4 cut(s) 119, 179, 204, 212
TfiI GAWTC 3 cut(s) 148, 406, 792
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 3 cut(s) 121, 179, 639
Tsp45I GTSAC 1 cut(s) 461
TspGWI ACGGA 3 cut(s) 30, 251, 278
Van91I CCANNNNNTGG 3 cut(s) 340, 526, 679
VpaK11BI GGWCC 2 cut(s) 157, 189
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 527
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.