Rmu_sc0001266.1_g000005

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001266.1
OG0002295
Physical Location & Seq
Reverse (-)
40562 .. 44356
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1488 bp
atgatgacccggatgctggttcggaacaacaacaacaacaactccggtctggaatccgggggcatagccttcgcatcgatttggtggtttgtctacatcggcttgtcgtgtttcttggtcctcttcgctggaataatgtcaggcttaactttaggactcatgtccctcggcctcgtcgacctcgagattctacagcgaagtggtactcccaccgagaagaagcaagcagcgactatacttccggtggttcagaagcagcatcagctgcttgtcactctgcttctgtgtaatgctggagctatggaagcgcttcctatatatttggacaagcttttcaatcagtatgttgctattattctgtctgtgacatttgtgctgtttttcggagaggttattccacaagcaatatgcactagatatggactggctgtgggttccaactttgtatggcttgtccgaattttgatgattatttgctacccaattgcttatccaattggaaagatcttggattgtgttctgggacataatgaggcattatttaggcgagctcagttaaaagcccttgtctccatccacagcaaggaggaaggcaaaggcggtgaacttacacatgatgagacaacaataattagtggagcactggacttaactgaaaagactgctgaggaggctatgacaccaattgaatcaaccttctccttagatgtcaattcaaagttggactgggaagcaatggggaaagttcttgctcggggacatagtcgagttcctgtctattctgggaattcaaggaatattattggacttctcctggtgaaaagtcttctcactgtacgacccgaaacagagacccctgtcagtgctgtttccatccgtagaatcccaagagttcccgcagatatgccactgtatgatatattgaatgagtttcaaaagggcagcagtcatatggctgctgtggtaaaggccagagggaaaagtaagactcatccacccacgattgacggagaaaataatgaaggaaacaaaattgctggcatggactcccaactgactactccattgctatccaagcaggatggaaagccagaccatgttgttgttgatgtgcccaagataccaaagcccactagtatgaaaaaggaagcactctcacactacaatgttggagaaacaattggccccccttcatcagaggatattgaggatggtgaagtcattggtatcatcaccctggaagatgtatttgaagaactcctgcaggaggagattgtcgatgaaacggatgaatatgttgatgtacataaaagaatacgcgttgctgcagctgcagctgcttcatcagtggcgcgagctccatcactgcggaggttaaatgtcaacaagggaccaggggcccaaactaagcaagtaaccccgaagaaatctactgatgaattggctgcaaggccacacgggacttttgctgatggaaacaagagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21

Protein Analysis

495

Amino Acids

53.97

Weight (kDa)

6.29

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 35 - 204 6.8e-35 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 93, 177
AccII CGCG 2 cut(s) 1320, 1354
AciI CCGC 3 cut(s) 600, 897, 1369
AcsI RAATTY 2 cut(s) 459, 787
AfaI GTAC 3 cut(s) 205, 837, 1305
AfeI AGCGCT 1 cut(s) 309
AfiI CCNNNNNNNGG 3 cut(s) 16, 583, 1267
AflIII ACRYGT 1 cut(s) 1318
AgsI TTSAA 7 cut(s) 337, 689, 717, 792, 925, 935, 1253
AhlI ACTAGT 1 cut(s) 1133
AjnI CCWGG 3 cut(s) 813, 1236, 1393
AluBI AGCT 7 cut(s) 265, 299, 331, 551, 1331, 1337, 1358
AluI AGCT 7 cut(s) 265, 299, 331, 551, 1331, 1337, 1358
Alw21I GWGCWC 3 cut(s) 553, 643, 1360
Alw26I GTCTC 3 cut(s) 574, 614, 845
Ama87I CYCGRG 2 cut(s) 182, 753
Aor51HI AGCGCT 1 cut(s) 309
AoxI GGCC 5 cut(s) 169, 969, 1183, 1398, 1451
ApaI GGGCCC 1 cut(s) 1402
ApoI RAATTY 2 cut(s) 459, 787
Asp700I GAANNNNTTC 1 cut(s) 309
AspLEI GCGC 2 cut(s) 310, 1354
AspS9I GGNCC 5 cut(s) 118, 1184, 1391, 1398, 1399
AsuC2I CCSGG 2 cut(s) 10, 58
AsuHPI GGTGA 4 cut(s) 614, 829, 1225, 1226
AvaI CYCGRG 2 cut(s) 182, 753
AvaII GGWCC 2 cut(s) 118, 1391
BaeGI GKGCMC 2 cut(s) 1116, 1402
BanII GRGCYC 3 cut(s) 553, 1360, 1402
BbsI GAAGAC 1 cut(s) 818
Bbv12I GWGCWC 3 cut(s) 553, 643, 1360
BbvCI CCTCAGC 1 cut(s) 666
BccI CCATC 6 cut(s) 581, 881, 1076, 1205, 1369, 1466
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 3 cut(s) 815, 1238, 1395
BcnI CCSGG 2 cut(s) 10, 58
BcoDI GTCTC 3 cut(s) 574, 614, 845
BcuI ACTAGT 1 cut(s) 1133
BfaI CTAG 2 cut(s) 414, 1134
BfmI CTRYAG 4 cut(s) 191, 1262, 1326, 1332
BfoI RGCGCY 1 cut(s) 311
BglII AGATCT 1 cut(s) 504
Bme1390I CCNGG 5 cut(s) 10, 58, 815, 1238, 1395
Bme18I GGWCC 2 cut(s) 118, 1391
BmeT110I CYCGRG 2 cut(s) 182, 753
BmgT120I GGNCC 5 cut(s) 118, 1184, 1391, 1398, 1399
BmiI GGNNCC 5 cut(s) 436, 1186, 1392, 1399, 1400
BmrFI CCNGG 5 cut(s) 10, 58, 815, 1238, 1395
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 3 cut(s) 3, 83, 268
BmuI ACTGGG 1 cut(s) 736
BoxI GACNNNNGTC 2 cut(s) 160, 857
BpiI GAAGAC 1 cut(s) 818
BpmI CTGGAG 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 666
BpuMI CCSGG 2 cut(s) 10, 58
Bsa29I ATCGAT 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 845
BsaJI CCNNGG 4 cut(s) 57, 166, 1236, 1394
BsaWI WCCGGW 2 cut(s) 44, 241
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 583, 1267
Bse1I ACTGG 3 cut(s) 429, 648, 731
Bse3DI GCAATG 2 cut(s) 741, 1064
BseBI CCWGG 3 cut(s) 815, 1238, 1395
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 57, 166, 1236, 1394
BseGI GGATG 7 cut(s) 18, 573, 873, 991, 1087, 1216, 1294
BseLI CCNNNNNNNGG 3 cut(s) 16, 583, 1267
BseMI GCAATG 2 cut(s) 741, 1064
BseMII CTCAG 2 cut(s) 566, 657
BseNI ACTGG 3 cut(s) 429, 648, 731
BseRI GAGGAG 2 cut(s) 683, 1283
BseSI GKGCMC 2 cut(s) 1116, 1402
Bsh1236I CGCG 2 cut(s) 1320, 1354
BshFI GGCC 5 cut(s) 171, 971, 1185, 1400, 1453
BshVI ATCGAT 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 553, 643, 1360
BsiHKCI CYCGRG 2 cut(s) 182, 753
BsiSI CCGG 4 cut(s) 10, 45, 57, 242
BslFI GGGAC 5 cut(s) 148, 537, 771, 1404, 1474
BslI CCNNNNNNNGG 3 cut(s) 16, 583, 1267
BsmAI GTCTC 3 cut(s) 574, 614, 845
BsmFI GGGAC 5 cut(s) 148, 537, 771, 1404, 1474
BsnI GGCC 5 cut(s) 171, 971, 1185, 1400, 1453
Bso31I GGTCTC 1 cut(s) 845
BsoBI CYCGRG 2 cut(s) 182, 753
Bsp120I GGGCCC 1 cut(s) 1398
Bsp1286I GDGCHC 5 cut(s) 553, 643, 1116, 1360, 1402
Bsp1407I TGTACA 1 cut(s) 1303
Bsp143I GATC 1 cut(s) 504
BspACI CCGC 3 cut(s) 600, 897, 1369
BspANI GGCC 5 cut(s) 171, 971, 1185, 1400, 1453
BspCNI CTCAG 2 cut(s) 565, 658
BspDI ATCGAT 1 cut(s) 77
BspFNI CGCG 2 cut(s) 1320, 1354
BspLI GGNNCC 5 cut(s) 436, 1186, 1392, 1399, 1400
BspMAI CTGCAG 3 cut(s) 1266, 1330, 1336
BspTNI GGTCTC 1 cut(s) 845
BsrDI GCAATG 2 cut(s) 741, 1064
BsrGI TGTACA 1 cut(s) 1303
BsrI ACTGG 3 cut(s) 429, 648, 731
BssECI CCNNGG 4 cut(s) 57, 166, 1236, 1394
BssMI GATC 1 cut(s) 504
Bst2UI CCWGG 3 cut(s) 815, 1238, 1395
Bst4CI ACNGT 2 cut(s) 835, 912
Bst6I CTCTTC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 265
BstAUI TGTACA 1 cut(s) 1303
BstC8I GCNNGC 4 cut(s) 225, 549, 1039, 1356
BstDEI CTNAG 4 cut(s) 552, 666, 703, 1407
BstENI CCTNNNNNAGG 1 cut(s) 1265
BstF5I GGATG 7 cut(s) 18, 573, 873, 991, 1087, 1216, 1294
BstFNI CGCG 2 cut(s) 1320, 1354
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 2 cut(s) 310, 1354
BstKTI GATC 1 cut(s) 507
BstMAI GTCTC 3 cut(s) 574, 614, 845
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 8 cut(s) 262, 265, 305, 671, 1075, 1331, 1334, 1337
BstNI CCWGG 3 cut(s) 815, 1238, 1395
BstPAI GACNNNNGTC 2 cut(s) 160, 857
BstSCI CCNGG 5 cut(s) 8, 56, 813, 1236, 1393
BstSFI CTRYAG 4 cut(s) 191, 1262, 1326, 1332
BstSLI GKGCMC 2 cut(s) 1116, 1402
BstUI CGCG 2 cut(s) 1320, 1354
BstV2I GAAGAC 1 cut(s) 818
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 5 cut(s) 171, 971, 1185, 1400, 1453
BsuTUI ATCGAT 1 cut(s) 77
BtsCI GGATG 7 cut(s) 18, 573, 873, 991, 1087, 1216, 1294
BtsI GCAGTG 1 cut(s) 1364
BtsIMutI CAGTG 6 cut(s) 641, 831, 868, 908, 1353, 1364
Cac8I GCNNGC 4 cut(s) 225, 549, 1039, 1356
CfoI GCGC 2 cut(s) 310, 1354
Cfr13I GGNCC 5 cut(s) 118, 1184, 1391, 1398, 1399
ClaI ATCGAT 1 cut(s) 77
Csp6I GTAC 3 cut(s) 204, 836, 1304
CviAII CATG 4 cut(s) 160, 614, 1042, 1097
CviQI GTAC 3 cut(s) 204, 836, 1304
DdeI CTNAG 4 cut(s) 552, 666, 703, 1407
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Ecl136II GAGCTC 2 cut(s) 551, 1358
Eco24I GRGCYC 3 cut(s) 553, 1360, 1402
Eco31I GGTCTC 1 cut(s) 845
Eco47I GGWCC 2 cut(s) 118, 1391
Eco47III AGCGCT 1 cut(s) 309
Eco53kI GAGCTC 2 cut(s) 551, 1358
Eco88I CYCGRG 2 cut(s) 182, 753
EcoICRI GAGCTC 2 cut(s) 551, 1358
EcoNI CCTNNNNNAGG 1 cut(s) 1265
EcoO109I RGGNCCY 1 cut(s) 1398
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 3 cut(s) 813, 1236, 1393
EcoT38I GRGCYC 3 cut(s) 553, 1360, 1402
FaeI CATG 4 cut(s) 163, 617, 1045, 1100
FaqI GGGAC 5 cut(s) 148, 537, 771, 1404, 1474
FatI CATG 4 cut(s) 159, 613, 1041, 1096
FauI CCCGC 1 cut(s) 904
FauNDI CATATG 1 cut(s) 951
FblI GTMKAC 2 cut(s) 93, 177
FokI GGATG 7 cut(s) 25, 560, 860, 978, 1094, 1223, 1301
FriOI GRGCYC 3 cut(s) 553, 1360, 1402
FspBI CTAG 2 cut(s) 414, 1134
GlaI GCGC 2 cut(s) 309, 1353
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 5 cut(s) 171, 971, 1185, 1400, 1453
HapII CCGG 4 cut(s) 10, 45, 57, 242
HhaI GCGC 2 cut(s) 310, 1354
Hin1II CATG 4 cut(s) 163, 617, 1045, 1100
Hin6I GCGC 2 cut(s) 308, 1352
HinP1I GCGC 2 cut(s) 308, 1352
HincII GTYRAC 2 cut(s) 178, 1384
HindII GTYRAC 2 cut(s) 178, 1384
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 7 cut(s) 53, 156, 187, 689, 882, 988, 1046
HpaII CCGG 4 cut(s) 10, 45, 57, 242
HphI GGTGA 4 cut(s) 614, 829, 1225, 1226
Hpy166II GTNNAC 4 cut(s) 94, 178, 605, 1384
Hpy188I TCNGA 5 cut(s) 24, 252, 386, 458, 1198
Hpy188III TCNNGA 2 cut(s) 50, 184
Hpy8I GTNNAC 4 cut(s) 94, 178, 605, 1384
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 5 cut(s) 79, 584, 706, 1016, 1200
HpyCH4III ACNGT 2 cut(s) 835, 912
HpyCH4V TGCA 5 cut(s) 411, 1264, 1328, 1334, 1448
HpyF10VI GCNNNNNNNGC 8 cut(s) 262, 265, 305, 671, 1075, 1331, 1334, 1337
HpyF3I CTNAG 4 cut(s) 552, 666, 703, 1407
Hsp92II CATG 4 cut(s) 163, 617, 1045, 1100
HspAI GCGC 2 cut(s) 308, 1352
Kzo9I GATC 1 cut(s) 504
LmnI GCTCC 3 cut(s) 296, 638, 1363
LweI GCATC 3 cut(s) 3, 83, 268
MaeI CTAG 2 cut(s) 414, 1134
MaeIII GTNAC 3 cut(s) 271, 364, 1414
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MboII GAAGA 6 cut(s) 115, 229, 818, 1253, 1265, 1435
MfeI CAATTG 4 cut(s) 483, 495, 684, 1179
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 5 cut(s) 553, 643, 1116, 1360, 1402
MluI ACGCGT 1 cut(s) 1318
MlyI GAGTC 3 cut(s) 150, 982, 1040
MmeI TCCRAC 3 cut(s) 462, 702, 1150
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 4 cut(s) 146, 557, 650, 1376
MslI CAYNNNNRTG 2 cut(s) 1136, 1164
MspA1I CMGCKG 3 cut(s) 265, 1331, 1337
MspI CCGG 4 cut(s) 10, 45, 57, 242
MspR9I CCNGG 5 cut(s) 10, 58, 815, 1238, 1395
MunI CAATTG 4 cut(s) 483, 495, 684, 1179
MvaI CCWGG 3 cut(s) 815, 1238, 1395
MvnI CGCG 2 cut(s) 1320, 1354
MwoI GCNNNNNNNGC 8 cut(s) 262, 265, 305, 671, 1075, 1331, 1334, 1337
NciI CCSGG 2 cut(s) 10, 58
NdeI CATATG 1 cut(s) 951
NdeII GATC 1 cut(s) 504
NlaIII CATG 4 cut(s) 163, 617, 1045, 1100
NlaIV GGNNCC 5 cut(s) 436, 1186, 1392, 1399, 1400
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 271, 364
PaeR7I CTCGAG 1 cut(s) 182
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 180
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 4 cut(s) 53, 187, 689, 882
PflFI GACNNNGTC 1 cut(s) 762
PleI GAGTC 3 cut(s) 150, 982, 1040
PpsI GAGTC 3 cut(s) 150, 982, 1040
PshAI GACNNNNGTC 2 cut(s) 160, 857
Psp124BI GAGCTC 2 cut(s) 553, 1360
Psp6I CCWGG 3 cut(s) 813, 1236, 1393
PspGI CCWGG 3 cut(s) 813, 1236, 1393
PspN4I GGNNCC 5 cut(s) 436, 1186, 1392, 1399, 1400
PspOMI GGGCCC 1 cut(s) 1398
PspPI GGNCC 5 cut(s) 118, 1184, 1391, 1398, 1399
PstI CTGCAG 3 cut(s) 1266, 1330, 1336
PsuI RGATCY 1 cut(s) 504
PsyI GACNNNGTC 1 cut(s) 762
PvuII CAGCTG 3 cut(s) 265, 1331, 1337
RsaI GTAC 3 cut(s) 205, 837, 1305
RsaNI GTAC 3 cut(s) 204, 836, 1304
RseI CAYNNNNRTG 2 cut(s) 1136, 1164
SacI GAGCTC 2 cut(s) 553, 1360
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 4 cut(s) 146, 557, 650, 1376
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 5 cut(s) 118, 1184, 1391, 1398, 1399
SbfI CCTGCAGG 1 cut(s) 1266
SchI GAGTC 3 cut(s) 150, 982, 1040
ScrFI CCNGG 5 cut(s) 10, 58, 815, 1238, 1395
SdaI CCTGCAGG 1 cut(s) 1266
SduI GDGCHC 5 cut(s) 553, 643, 1116, 1360, 1402
SfaNI GCATC 3 cut(s) 3, 83, 268
SfcI CTRYAG 4 cut(s) 191, 1262, 1326, 1332
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 2 cut(s) 118, 1391
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 1136, 1164
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SpeI ACTAGT 1 cut(s) 1133
Sse8387I CCTGCAGG 1 cut(s) 1266
SsiI CCGC 3 cut(s) 600, 897, 1369
SspI AATATT 1 cut(s) 799
SspMI CTAG 2 cut(s) 414, 1134
SstI GAGCTC 2 cut(s) 553, 1360
StyD4I CCNGG 5 cut(s) 8, 56, 813, 1236, 1393
TaaI ACNGT 2 cut(s) 835, 912
TaqI TCGA 5 cut(s) 77, 177, 183, 766, 1278
TatI WGTACW 1 cut(s) 1303
TfiI GAWTC 4 cut(s) 53, 187, 689, 882
Tru1I TTAA 4 cut(s) 146, 557, 650, 1376
Tru9I TTAA 4 cut(s) 146, 557, 650, 1376
TscAI CASTG 6 cut(s) 648, 838, 868, 915, 1353, 1371
TseFI GTSAC 2 cut(s) 271, 364
Tsp45I GTSAC 2 cut(s) 271, 364
TspDTI ATGAA 7 cut(s) 1035, 1154, 1182, 1296, 1305, 1332, 1452
TspGWI ACGGA 3 cut(s) 866, 1023, 1301
TspRI CASTG 6 cut(s) 648, 838, 868, 915, 1353, 1371
Tth111I GACNNNGTC 1 cut(s) 762
VpaK11BI GGWCC 2 cut(s) 118, 1391
XagI CCTNNNNNAGG 1 cut(s) 1265
XapI RAATTY 2 cut(s) 459, 787
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 2 cut(s) 93, 177
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 2 cut(s) 414, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.