Rmu_sc0001940.1_g000003

Cysteine desulfurase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001940.1
OG0002608
Physical Location & Seq
Forward (+)
10743 .. 11724
982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 822 bp
atgagactccacagtcccaaaaaccaccccgacaccgtctaccacaaacccaccaaaaaccatgggattgtggaagccactctcactcgatctactcttcccttctcagtgcgattctctctccacacccaccaaacattcatcaccacccagacggagcacaagtgcgtcctcgactcttgcctccatctccagcaggagggtttcgacatcacctaccttcctgtgaaggccgacgggatcatcgacctgaaccagctccgggtcgagatccggccctataccggactggtctcagttatggcagtgaacaatgaaatcggtgtgattcaacctatggaggaaatcggagatatctgcaaggagttcaagattcagtttcacactgatgctgctcaggcgttagggaagattccgattgatgtggataggtggaatgtgagcttgatgtcgttgagtgggcacaagatgtatgggcctaagggagttggggcattgtacatgaggcggcggctgaggattcgggtggagcctcagatgaatggaggtgggcaggagagggggataaggagcgggacggttccgacgccgttggttgtggggatggggactgcttgcgagattgcgaagaaggagatggagtatgatcagaagaggattagtcagctgcaggagaggatgctgagtgggattagggagcaactggatgaggttggtggtgttggtggtgttgaagctttgctggtgttgaaggttttgctggttggttctatatttaatcccgctggcaccaaacatgggtcaaggcactccatagcataa

Protein Analysis

273

Amino Acids

30.32

Weight (kDa)

8.84

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 35 - 233 1e-47 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 788
AccBSI CCGCTC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 39
AciI CCGC 4 cut(s) 508, 511, 573, 783
AclWI GGATC 2 cut(s) 248, 265
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 4 cut(s) 199, 262, 284, 798
AgsI TTSAA 4 cut(s) 332, 370, 734, 751
AluBI AGCT 4 cut(s) 259, 444, 667, 737
AluI AGCT 4 cut(s) 259, 444, 667, 737
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 2 cut(s) 248, 265
AoxI GGCC 3 cut(s) 231, 275, 476
ApeKI GCWGC 2 cut(s) 392, 667
AspS9I GGNCC 2 cut(s) 276, 476
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 136, 205
AxyI CCTNAGG 1 cut(s) 480
BaeGI GKGCMC 1 cut(s) 465
BanI GGYRCC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 515
BbvI GCAGC 2 cut(s) 379, 654
BccI CCATC 3 cut(s) 195, 598, 631
BceAI ACGGC 1 cut(s) 574
BclI TGATCA 1 cut(s) 646
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 298
BfmI CTRYAG 1 cut(s) 668
BisI GCNGC 4 cut(s) 393, 509, 512, 668
BlsI GCNGC 4 cut(s) 394, 510, 513, 669
Bme1390I CCNGG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 276, 476
BmiI GGNNCC 3 cut(s) 531, 582, 790
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 2 cut(s) 379, 669
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 2 cut(s) 396, 515
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 626, 656
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 262, 284, 798
Bse1I ACTGG 2 cut(s) 294, 708
Bse21I CCTNAGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 3 cut(s) 609, 684, 712
BseLI CCNNNNNNNGG 4 cut(s) 199, 262, 284, 798
BseMII CTCAG 6 cut(s) 120, 309, 410, 506, 548, 674
BseNI ACTGG 2 cut(s) 294, 708
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 2 cut(s) 379, 654
BshFI GGCC 3 cut(s) 233, 277, 478
BshNI GGYRCC 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 3 cut(s) 262, 274, 285
BslFI GGGAC 2 cut(s) 589, 622
BslI CCNNNNNNNGG 4 cut(s) 199, 262, 284, 798
BsmAI GTCTC 1 cut(s) 298
BsmFI GGGAC 2 cut(s) 589, 622
BsnI GGCC 3 cut(s) 233, 277, 478
Bso31I GGTCTC 1 cut(s) 298
Bsp1286I GDGCHC 2 cut(s) 162, 465
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 4 cut(s) 89, 240, 270, 646
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 4 cut(s) 508, 511, 573, 783
BspANI GGCC 3 cut(s) 233, 277, 478
BspCNI CTCAG 6 cut(s) 119, 308, 409, 507, 547, 675
BspLI GGNNCC 3 cut(s) 531, 582, 790
BspMAI CTGCAG 1 cut(s) 672
BspPI GGATC 2 cut(s) 248, 265
BspT107I GGYRCC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 298
BsrBI CCGCTC 1 cut(s) 573
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 294, 708
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 4 cut(s) 89, 240, 270, 646
BssNI GRCGYC 1 cut(s) 587
BssT1I CCWWGG 1 cut(s) 61
Bst4CI ACNGT 3 cut(s) 14, 37, 580
Bst6I CTCTTC 2 cut(s) 102, 647
BstACI GRCGYC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 616, 787
BstDEI CTNAG 7 cut(s) 106, 295, 396, 480, 515, 534, 683
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 3 cut(s) 609, 684, 712
BstKTI GATC 4 cut(s) 92, 243, 273, 649
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 4 cut(s) 89, 240, 270, 646
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 668
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 2 cut(s) 379, 654
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 480
BsuRI GGCC 3 cut(s) 233, 277, 478
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 3 cut(s) 609, 684, 712
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 114, 312, 384
Cac8I GCNNGC 2 cut(s) 616, 787
Cfr13I GGNCC 2 cut(s) 276, 476
CseI GACGC 2 cut(s) 157, 595
Csp6I GTAC 1 cut(s) 499
CviAII CATG 3 cut(s) 62, 502, 797
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 7 cut(s) 106, 295, 396, 480, 515, 534, 683
DpnI GATC 4 cut(s) 91, 242, 272, 648
DpnII GATC 4 cut(s) 89, 240, 270, 646
DrdI GACNNNNNNGTC 1 cut(s) 12
DseDI GACNNNNNNGTC 1 cut(s) 12
Eam1104I CTCTTC 2 cut(s) 102, 647
EarI CTCTTC 2 cut(s) 102, 647
Eco130I CCWWGG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 298
Eco32I GATATC 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 3 cut(s) 65, 505, 800
FaqI GGGAC 2 cut(s) 589, 622
FatI CATG 3 cut(s) 61, 501, 796
FauI CCCGC 2 cut(s) 566, 790
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 393, 509, 512, 668
FokI GGATG 3 cut(s) 616, 691, 719
Fsp4HI GCNGC 4 cut(s) 393, 509, 512, 668
GluI GCNGC 4 cut(s) 393, 509, 512, 668
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 3 cut(s) 233, 277, 478
HapII CCGG 3 cut(s) 262, 274, 285
HgaI GACGC 2 cut(s) 157, 595
Hin1I GRCGYC 1 cut(s) 587
Hin1II CATG 3 cut(s) 65, 505, 800
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 7 cut(s) 6, 114, 176, 328, 373, 412, 520
HpaII CCGG 3 cut(s) 262, 274, 285
HphI GGTGA 2 cut(s) 136, 205
Hpy166II GTNNAC 2 cut(s) 40, 310
Hpy188I TCNGA 5 cut(s) 350, 417, 537, 585, 651
Hpy188III TCNNGA 2 cut(s) 268, 370
Hpy8I GTNNAC 2 cut(s) 40, 310
Hpy99I CGWCG 2 cut(s) 239, 589
HpyAV CCTTC 5 cut(s) 112, 223, 230, 625, 745
HpyCH4III ACNGT 3 cut(s) 14, 37, 580
HpyCH4V TGCA 2 cut(s) 360, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 7 cut(s) 106, 295, 396, 480, 515, 534, 683
Hsp92I GRCGYC 1 cut(s) 587
Hsp92II CATG 3 cut(s) 65, 505, 800
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 4 cut(s) 89, 240, 270, 646
LmnI GCTCC 5 cut(s) 157, 264, 529, 570, 697
Lsp1109I GCAGC 2 cut(s) 379, 654
LweI GCATC 2 cut(s) 379, 669
MalI GATC 4 cut(s) 91, 242, 272, 648
MbiI CCGCTC 1 cut(s) 573
MboI GATC 4 cut(s) 89, 240, 270, 646
MboII GAAGA 4 cut(s) 89, 421, 640, 664
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 2 cut(s) 162, 465
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 608
MseI TTAA 1 cut(s) 777
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 2 cut(s) 667, 785
MspI CCGG 3 cut(s) 262, 274, 285
MspR9I CCNGG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 263
NcoI CCATGG 1 cut(s) 61
NdeII GATC 4 cut(s) 89, 240, 270, 646
NlaIII CATG 3 cut(s) 65, 505, 800
NlaIV GGNNCC 3 cut(s) 531, 582, 790
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 5 cut(s) 114, 328, 373, 412, 520
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 4 cut(s) 394, 510, 513, 669
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
PspN4I GGNNCC 3 cut(s) 531, 582, 790
PspPI GGNCC 2 cut(s) 276, 476
PstI CTGCAG 1 cut(s) 672
PsuI RGATCY 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 4 cut(s) 393, 509, 512, 668
Sau3AI GATC 4 cut(s) 89, 240, 270, 646
Sau96I GGNCC 2 cut(s) 276, 476
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 2 cut(s) 162, 465
SfaNI GCATC 2 cut(s) 379, 669
SfcI CTRYAG 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 387
SsiI CCGC 4 cut(s) 508, 511, 573, 783
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 3 cut(s) 14, 37, 580
TaqI TCGA 5 cut(s) 88, 174, 207, 246, 267
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 511, 514
TfiI GAWTC 5 cut(s) 114, 328, 373, 412, 520
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 3 cut(s) 114, 312, 391
TseI GCWGC 2 cut(s) 392, 667
TspDTI ATGAA 3 cut(s) 130, 330, 554
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 114, 312, 391
Tth111I GACNNNGTC 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.