Rmu_sc0002132.1_g000004

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002132.1
OG0002812
Physical Location & Seq
Forward (+)
7978 .. 11314
3337 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
atgatggattcggagccatcacacaccaggaatgccgccaacactagcctgcgtccactatccccatcccaaaatgacaaaagtgttttagaaggttttgctgatattgggtatctctctgacccacacaaagttcacaatggaagccctgatttctctcaaacctgcgatgccaaattggaagtcgttgacgacggtgccggcgccggagccggagccgaatcggtcctcgacgtctcctgccaaagcctggactttccgatcggtgagaactccggtgcgtacaatttactgcctaaatccatcgccggactaaaaggcttgaagacgctgaggtttttcagcaacgagatcaagctgttctcgtcgcccgagatcggcggcttcgtaaggctagaatgcgtgaggatctcgtcgccggagttcgacggtttgccgctgcacaaattcaaaaggatgaaggggcttgagctctccatggtgacatctcggtgctcggcggttcccattttgagcgagatcgccggactcaagctcctccgcacaaagccatcggaattgcctgagggtttgtccagattagctgggttggggaatttggacttgttgcataataggctgacgtcattgggggatgttgattttgggttgaatgttagggtccagaatttgaatctgaagtacaataggcttccgactattttagaagccagagatgggatggatgtaaatgatgtaaatcaactgctggattctgtacaagataatagtggcaactcaaacttagaggctgctcctcagcgacagaaagaagatgctgataatggtgttgaccacacaaagttgacagggaggaagaaaaaatggaaggaattgcagaagaagagcatacatgatacacaagagatggccgaggcaaaatacaagagatggaaaaagaaacctgcaccatgtggctgggatgtttttaattcgaaaacattgttcaatgcacacaaaaaacgaacaaacaagatcgaagttgacatggaagaatacaataagatgaaacaggaaggagcttttagctgcctcgaaaatggaaagactccgaaggtctcggaggagaaagttgacaagatgttgcaggagctcaaggaactcgaagagaagaaaaagaaatttagcaggaggaggaggttccatgaggacaaggatattgactcgatcaatgaccgcaatgagcatttcaacaagaagattgacagagcctttggtaagtacacattggaaatcaaaaacaatctcgagcgaggtactgcgttgcctgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

437

Amino Acids

49.04

Weight (kDa)

8.5

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYF2 PF08231 292 - 430 9.6e-30 SYF2 splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 237, 626
Acc36I ACCTGC 2 cut(s) 173, 952
AccB1I GGYRCC 2 cut(s) 197, 203
AccB7I CCANNNNNTGG 1 cut(s) 250
AciI CCGC 6 cut(s) 36, 381, 437, 500, 541, 1216
AclWI GGATC 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 909
AcsI RAATTY 4 cut(s) 446, 595, 667, 1160
AcuI CTGAAG 1 cut(s) 698
AcyI GRCGYC 3 cut(s) 204, 234, 623
AdeI CACNNNGTG 1 cut(s) 953
AfaI GTAC 5 cut(s) 284, 683, 759, 1262, 1297
AfiI CCNNNNNNNGG 3 cut(s) 250, 377, 717
AgsI TTSAA 6 cut(s) 325, 451, 652, 673, 988, 1231
AjnI CCWGG 2 cut(s) 26, 249
AluBI AGCT 7 cut(s) 358, 472, 535, 584, 1061, 1068, 1132
AluI AGCT 7 cut(s) 358, 472, 535, 584, 1061, 1068, 1132
Alw21I GWGCWC 3 cut(s) 474, 497, 1134
Alw26I GTCTC 2 cut(s) 241, 1102
AlwI GGATC 1 cut(s) 416
Ama87I CYCGRG 2 cut(s) 371, 1286
AoxI GGCC 1 cut(s) 909
ApeKI GCWGC 3 cut(s) 439, 791, 1068
ApoI RAATTY 4 cut(s) 446, 595, 667, 1160
ArsI GACNNNNNNTTYG 4 cut(s) 168, 200, 1235, 1267
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 226, 661
AsuHPI GGTGA 2 cut(s) 278, 493
AsuII TTCGAA 1 cut(s) 974
AvaI CYCGRG 2 cut(s) 371, 1286
AvaII GGWCC 2 cut(s) 226, 661
AxyI CCTNAGG 1 cut(s) 564
BanI GGYRCC 2 cut(s) 197, 203
BanII GRGCYC 2 cut(s) 474, 1134
BbsI GAAGAC 1 cut(s) 332
Bbv12I GWGCWC 3 cut(s) 474, 497, 1134
BbvCI CCTCAGC 2 cut(s) 332, 798
BbvI GCAGC 3 cut(s) 426, 778, 1055
BccI CCATC 8 cut(s) 25, 73, 311, 559, 710, 715, 901, 924
BciT130I CCWGG 2 cut(s) 28, 251
BcoDI GTCTC 2 cut(s) 241, 1102
BfaI CTAG 2 cut(s) 45, 395
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 2 cut(s) 173, 952
BisI GCNGC 6 cut(s) 36, 382, 437, 440, 792, 1069
BlsI GCNGC 6 cut(s) 37, 383, 438, 441, 793, 1070
Bme1390I CCNGG 2 cut(s) 28, 251
Bme18I GGWCC 2 cut(s) 226, 661
BmeT110I CYCGRG 2 cut(s) 371, 1286
BmgT120I GGNCC 2 cut(s) 226, 661
BmiI GGNNCC 8 cut(s) 15, 199, 205, 211, 217, 504, 662, 1181
BmrFI CCNGG 2 cut(s) 28, 251
BmsI GCATC 2 cut(s) 160, 805
BpiI GAAGAC 1 cut(s) 332
Bpu10I CCTNAGC 2 cut(s) 332, 798
Bpu14I TTCGAA 1 cut(s) 974
BpuEI CTTGAG 3 cut(s) 488, 515, 1118
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 3 cut(s) 204, 234, 623
BsaI GGTCTC 1 cut(s) 1102
BsaJI CCNNGG 2 cut(s) 477, 912
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 377, 717
Bse118I RCCGGY 1 cut(s) 200
Bse21I CCTNAGG 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 1225
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 2 cut(s) 28, 251
BseDI CCNNGG 2 cut(s) 477, 912
BseGI GGATG 6 cut(s) 65, 462, 640, 726, 730, 967
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 250, 377, 717
BseMI GCAATG 1 cut(s) 1225
BseMII CTCAG 3 cut(s) 323, 555, 812
BseRI GAGGAG 5 cut(s) 527, 786, 1118, 1186, 1189
BseXI GCAGC 3 cut(s) 426, 778, 1055
BseYI CCCAGC 2 cut(s) 584, 957
BsgI GTGCAG 2 cut(s) 425, 930
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 911
BshNI GGYRCC 2 cut(s) 197, 203
BsiEI CGRYCG 1 cut(s) 264
BsiHKAI GWGCWC 3 cut(s) 474, 497, 1134
BsiHKCI CYCGRG 2 cut(s) 371, 1286
BsiSI CCGG 7 cut(s) 201, 207, 213, 276, 309, 419, 525
BslI CCNNNNNNNGG 3 cut(s) 250, 377, 717
BsmAI GTCTC 2 cut(s) 241, 1102
BsmBI CGTCTC 1 cut(s) 241
BsmI GAATGC 2 cut(s) 37, 404
BsnI GGCC 1 cut(s) 911
Bso31I GGTCTC 1 cut(s) 1102
BsoBI CYCGRG 2 cut(s) 371, 1286
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 3 cut(s) 474, 497, 1134
Bsp1407I TGTACA 1 cut(s) 757
Bsp143I GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 6 cut(s) 36, 381, 437, 500, 541, 1216
BspANI GGCC 1 cut(s) 911
BspCNI CTCAG 3 cut(s) 324, 556, 811
BspLI GGNNCC 8 cut(s) 15, 199, 205, 211, 217, 504, 662, 1181
BspMI ACCTGC 2 cut(s) 173, 952
BspPI GGATC 1 cut(s) 416
BspQI GCTCTTC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 974
BspT107I GGYRCC 2 cut(s) 197, 203
BspTNI GGTCTC 1 cut(s) 1102
BsrDI GCAATG 1 cut(s) 1225
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 757
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 477, 912
BssMI GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
BssNI GRCGYC 3 cut(s) 204, 234, 623
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 28, 251
Bst4CI ACNGT 2 cut(s) 197, 431
Bst6I CTCTTC 2 cut(s) 878, 1140
BstACI GRCGYC 3 cut(s) 204, 234, 623
BstAUI TGTACA 1 cut(s) 757
BstBI TTCGAA 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 50, 202
BstDEI CTNAG 4 cut(s) 332, 564, 784, 798
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 6 cut(s) 65, 462, 640, 726, 730, 967
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 7 cut(s) 264, 354, 378, 411, 522, 1017, 1209
BstMAI GTCTC 2 cut(s) 241, 1102
BstMBI GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 1 cut(s) 616
BstNI CCWGG 2 cut(s) 28, 251
BstSCI CCNGG 2 cut(s) 26, 249
BstV1I GCAGC 3 cut(s) 426, 778, 1055
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 1 cut(s) 408
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 564
BsuRI GGCC 1 cut(s) 911
BtgI CCRYGG 1 cut(s) 477
BtgZI GCGATG 2 cut(s) 183, 289
BtsCI GGATG 6 cut(s) 65, 462, 640, 726, 730, 967
BveI ACCTGC 2 cut(s) 173, 952
Cac8I GCNNGC 2 cut(s) 50, 202
CfoI GCGC 1 cut(s) 206
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 226, 661
CseI GACGC 2 cut(s) 41, 337
Csp6I GTAC 5 cut(s) 283, 682, 758, 1261, 1296
CviAII CATG 5 cut(s) 478, 893, 951, 1027, 1184
CviQI GTAC 5 cut(s) 283, 682, 758, 1261, 1296
DdeI CTNAG 4 cut(s) 332, 564, 784, 798
DinI GGCGCC 1 cut(s) 205
DpnI GATC 7 cut(s) 263, 353, 377, 410, 521, 1016, 1208
DpnII GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
DraIII CACNNNGTG 1 cut(s) 953
EaeI YGGCCR 1 cut(s) 909
Eam1104I CTCTTC 2 cut(s) 878, 1140
EarI CTCTTC 2 cut(s) 878, 1140
Ecl136II GAGCTC 2 cut(s) 472, 1132
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 474, 1134
Eco31I GGTCTC 1 cut(s) 1102
Eco47I GGWCC 2 cut(s) 226, 661
Eco53kI GAGCTC 2 cut(s) 472, 1132
Eco57I CTGAAG 1 cut(s) 698
Eco81I CCTNAGG 1 cut(s) 564
Eco88I CYCGRG 2 cut(s) 371, 1286
EcoICRI GAGCTC 2 cut(s) 472, 1132
EcoRII CCWGG 2 cut(s) 26, 249
EcoT14I CCWWGG 1 cut(s) 477
EcoT38I GRGCYC 2 cut(s) 474, 1134
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 5 cut(s) 481, 896, 954, 1030, 1187
FaiI YATR 7 cut(s) 479, 612, 890, 894, 952, 1028, 1185
FatI CATG 5 cut(s) 477, 892, 950, 1026, 1183
Fnu4HI GCNGC 6 cut(s) 36, 382, 437, 440, 792, 1069
FokI GGATG 6 cut(s) 52, 469, 647, 733, 737, 974
FriOI GRGCYC 2 cut(s) 474, 1134
Fsp4HI GCNGC 6 cut(s) 36, 382, 437, 440, 792, 1069
FspBI CTAG 2 cut(s) 45, 395
GlaI GCGC 1 cut(s) 205
GluI GCNGC 6 cut(s) 36, 382, 437, 440, 792, 1069
GsaI CCCAGC 2 cut(s) 588, 961
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 1 cut(s) 911
HapII CCGG 7 cut(s) 201, 207, 213, 276, 309, 419, 525
HgaI GACGC 2 cut(s) 41, 337
HhaI GCGC 1 cut(s) 206
Hin1I GRCGYC 3 cut(s) 204, 234, 623
Hin1II CATG 5 cut(s) 481, 896, 954, 1030, 1187
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HincII GTYRAC 5 cut(s) 190, 832, 846, 1024, 1114
HindII GTYRAC 5 cut(s) 190, 832, 846, 1024, 1114
HinfI GANTC 7 cut(s) 8, 221, 528, 673, 752, 1087, 1202
HpaII CCGG 7 cut(s) 201, 207, 213, 276, 309, 419, 525
HphI GGTGA 2 cut(s) 278, 493
Hpy166II GTNNAC 8 cut(s) 56, 136, 190, 832, 846, 1024, 1114, 1263
Hpy188I TCNGA 8 cut(s) 13, 121, 261, 556, 678, 696, 1092, 1102
Hpy188III TCNNGA 3 cut(s) 576, 664, 1286
Hpy8I GTNNAC 8 cut(s) 56, 136, 190, 832, 846, 1024, 1114, 1263
Hpy99I CGWCG 5 cut(s) 197, 236, 370, 418, 431
HpyAV CCTTC 5 cut(s) 86, 454, 862, 1049, 1087
HpyCH4III ACNGT 2 cut(s) 197, 431
HpyCH4IV ACGT 2 cut(s) 234, 623
HpyCH4V TGCA 6 cut(s) 442, 610, 877, 947, 992, 1126
HpyF10VI GCNNNNNNNGC 1 cut(s) 616
HpyF3I CTNAG 4 cut(s) 332, 564, 784, 798
HpySE526I ACGT 2 cut(s) 234, 623
Hsp92I GRCGYC 3 cut(s) 204, 234, 623
Hsp92II CATG 5 cut(s) 481, 896, 954, 1030, 1187
HspAI GCGC 1 cut(s) 204
KasI GGCGCC 1 cut(s) 203
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 7 cut(s) 13, 209, 215, 540, 799, 1058, 1129
Lsp1109I GCAGC 3 cut(s) 426, 778, 1055
LweI GCATC 2 cut(s) 160, 805
MaeI CTAG 2 cut(s) 45, 395
MaeII ACGT 2 cut(s) 234, 623
MaeIII GTNAC 1 cut(s) 481
MalI GATC 7 cut(s) 263, 353, 377, 410, 521, 1016, 1208
MboI GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
MboII GAAGA 9 cut(s) 337, 824, 868, 892, 895, 1043, 1157, 1162, 1249
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 3 cut(s) 474, 497, 1134
MluCI AATT 9 cut(s) 176, 286, 446, 557, 595, 667, 872, 970, 1160
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 3 cut(s) 522, 1081, 1196
MmeI TCCRAC 1 cut(s) 719
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 2 cut(s) 969, 1312
MslI CAYNNNNRTG 1 cut(s) 490
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 7 cut(s) 201, 207, 213, 276, 309, 419, 525
MspR9I CCNGG 2 cut(s) 28, 251
Mva1269I GAATGC 2 cut(s) 37, 404
MvaI CCWGG 2 cut(s) 28, 251
MwoI GCNNNNNNNGC 1 cut(s) 616
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 204
NcoI CCATGG 1 cut(s) 477
NdeII GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 5 cut(s) 481, 896, 954, 1030, 1187
NlaIV GGNNCC 8 cut(s) 15, 199, 205, 211, 217, 504, 662, 1181
NmeAIII GCCGAG 2 cut(s) 476, 937
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 974
PaeR7I CTCGAG 1 cut(s) 1286
PciSI GCTCTTC 1 cut(s) 878
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PctI GAATGC 2 cut(s) 37, 404
PdiI GCCGGC 1 cut(s) 202
PfeI GAWTC 4 cut(s) 8, 221, 673, 752
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 6 cut(s) 37, 383, 438, 441, 793, 1070
Ple19I CGATCG 1 cut(s) 264
PleI GAGTC 3 cut(s) 522, 1081, 1196
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 3 cut(s) 522, 1081, 1196
Psp124BI GAGCTC 2 cut(s) 474, 1134
Psp6I CCWGG 2 cut(s) 26, 249
PspFI CCCAGC 2 cut(s) 584, 957
PspGI CCWGG 2 cut(s) 26, 249
PspN4I GGNNCC 8 cut(s) 15, 199, 205, 211, 217, 504, 662, 1181
PspPI GGNCC 2 cut(s) 226, 661
PsuI RGATCY 1 cut(s) 408
PvuI CGATCG 1 cut(s) 264
RsaI GTAC 5 cut(s) 284, 683, 759, 1262, 1297
RsaNI GTAC 5 cut(s) 283, 682, 758, 1261, 1296
RseI CAYNNNNRTG 1 cut(s) 490
SacI GAGCTC 2 cut(s) 474, 1134
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 2 cut(s) 969, 1312
SatI GCNGC 6 cut(s) 36, 382, 437, 440, 792, 1069
Sau3AI GATC 7 cut(s) 261, 351, 375, 408, 519, 1014, 1206
Sau96I GGNCC 2 cut(s) 226, 661
SchI GAGTC 3 cut(s) 522, 1081, 1196
ScrFI CCNGG 2 cut(s) 28, 251
SduI GDGCHC 3 cut(s) 474, 497, 1134
SfaNI GCATC 2 cut(s) 160, 805
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 1286
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 2 cut(s) 226, 661
SlaI CTCGAG 1 cut(s) 1286
SmiMI CAYNNNNRTG 1 cut(s) 490
SmlI CTYRAG 4 cut(s) 467, 530, 1133, 1286
SmoI CTYRAG 4 cut(s) 467, 530, 1133, 1286
Sse9I AATT 9 cut(s) 176, 286, 446, 557, 595, 667, 872, 970, 1160
SsiI CCGC 6 cut(s) 36, 381, 437, 500, 541, 1216
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 2 cut(s) 45, 395
SstI GAGCTC 2 cut(s) 474, 1134
StyD4I CCNGG 2 cut(s) 26, 249
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 2 cut(s) 197, 431
TaiI ACGT 2 cut(s) 237, 626
TaqI TCGA 8 cut(s) 231, 426, 974, 1017, 1074, 1143, 1205, 1287
TaqII GACCGA 1 cut(s) 214
TasI AATT 9 cut(s) 176, 286, 446, 557, 595, 667, 872, 970, 1160
TatI WGTACW 3 cut(s) 681, 757, 1260
TauI GCSGC 3 cut(s) 38, 384, 439
TfiI GAWTC 4 cut(s) 8, 221, 673, 752
Tru1I TTAA 2 cut(s) 969, 1312
Tru9I TTAA 2 cut(s) 969, 1312
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 3 cut(s) 439, 791, 1068
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 473, 1061
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 226, 661
XapI RAATTY 4 cut(s) 446, 595, 667, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XhoI CTCGAG 1 cut(s) 1286
XspI CTAG 2 cut(s) 45, 395
ZraI GACGTC 2 cut(s) 235, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.