Rmu_sc0002299.1_g000025

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002299.1
OG0003259
Physical Location & Seq
Forward (+)
113899 .. 115915
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
atgtcttggctcttcacctctctccaacccgacgcggaaaacgacgcgtcgtttctcggccaaaccctcacccgccaactccgcggcgtcgccaccttcctcgctccgccgccctcctcctccaagtcccttccctcctccgattctccaccgccgtcgccgtcgccgtcgccggccctcttgggagtccgaaacgatctcgcggaattgggcgggagctttaagaccggcctctcgcttctctccaccggcctctcgcttctctccgtcgccggaatctccaaattggcttcgaacttgctcccggttcggaacggagctccggaagaaggcggcggcggcggtcccgtcccggggattaccgatgagattctcggcttcgtcgctcagatttcgaagcttccggactgttggaccggctttcctataccactggaccaccatgattttagcatgtccaatgctcagagaaaacatgcctccactgtcgaacaattggttccggggtttgctgatctgagagtcagagtttgcagttcgatgagtgaggagcaattttggatgatctatttcttgttgctgcttccaagactaaatgcagatgattttgagcttctgtcaacccccaagattgttgaagcaagaaatgtacttctgcacaagcttcaaaagaacacgcagctatccacctctgaggagtcaactcttcattcaactgaaaaggacacccaggttgagaagacacaagggggagaattctcctctgcagaaaagaaagatctgacagagattgtaaatacagctgaaaggttaagaatcgacgatgaagagagcactgagcaatggtccgaagcagcgagcattagttctggtacttttgtggatgacccaaaaaaacttgaacatgaagacgatatctcattcagcgatttagaggatgacgagaatgacatttctactagatcgtcaggcctaaggccagcaaaggacgttagggaatgttcacctagtggatcaaatcattcaaatgactgggttcggctgaatagaagtgctgcgattgatggtggccatcaaaggactggtacatcgaaagagaaagattcagaaggtgaggagtcgaatgactggcttactgtagataagtttgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

384

Amino Acids

41.74

Weight (kDa)

4.65

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 149 - 199 2.7e-06 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 35, 47, 84, 203
AccIII TCCGGA 2 cut(s) 322, 403
AclWI GGATC 1 cut(s) 1021
AcoI YGGCCR 2 cut(s) 58, 1069
AcsI RAATTY 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 1010
AfaI GTAC 3 cut(s) 651, 874, 1087
AfiI CCNNNNNNNGG 3 cut(s) 329, 353, 354
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 5 cut(s) 638, 668, 714, 902, 1026
AjnI CCWGG 1 cut(s) 729
AluBI AGCT 7 cut(s) 219, 320, 400, 613, 664, 682, 803
AluI AGCT 7 cut(s) 219, 320, 400, 613, 664, 682, 803
Alw21I GWGCWC 2 cut(s) 322, 836
AlwI GGATC 1 cut(s) 1021
Ama87I CYCGRG 1 cut(s) 352
Aor13HI TCCGGA 2 cut(s) 322, 403
AoxI GGCC 7 cut(s) 58, 174, 229, 250, 970, 977, 1069
ApeKI GCWGC 4 cut(s) 580, 679, 854, 1055
ApoI RAATTY 1 cut(s) 755
AspS9I GGNCC 5 cut(s) 175, 344, 414, 436, 846
AsuC2I CCSGG 4 cut(s) 305, 353, 354, 504
AsuHPI GGTGA 4 cut(s) 7, 61, 996, 1124
AsuII TTCGAA 2 cut(s) 293, 395
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 4 cut(s) 344, 414, 436, 846
AxyI CCTNAGG 1 cut(s) 974
BaeI ACNNNNGTAYC 2 cut(s) 864, 897
BalI TGGCCA 1 cut(s) 1071
BanII GRGCYC 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 746, 915
Bbv12I GWGCWC 2 cut(s) 322, 836
BbvI GCAGC 4 cut(s) 567, 691, 866, 1042
BccI CCATC 2 cut(s) 1058, 1080
BceAI ACGGC 3 cut(s) 139, 145, 151
BciT130I CCWGG 1 cut(s) 731
BcnI CCSGG 4 cut(s) 305, 353, 354, 504
BfaI CTAG 2 cut(s) 960, 1008
BfmI CTRYAG 2 cut(s) 765, 1137
BglII AGATCT 1 cut(s) 778
BisI GCNGC 9 cut(s) 85, 110, 334, 337, 340, 581, 680, 855, 1056
BlsI GCNGC 9 cut(s) 86, 111, 335, 338, 341, 582, 681, 856, 1057
Bme1390I CCNGG 5 cut(s) 305, 353, 354, 504, 731
Bme18I GGWCC 4 cut(s) 344, 414, 436, 846
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 5 cut(s) 175, 344, 414, 436, 846
BmiI GGNNCC 2 cut(s) 346, 501
BmrFI CCNGG 5 cut(s) 305, 353, 354, 504, 731
BmrI ACTGGG 1 cut(s) 1042
BmuI ACTGGG 1 cut(s) 1042
BpiI GAAGAC 2 cut(s) 746, 915
Bpu14I TTCGAA 2 cut(s) 293, 395
BpuMI CCSGG 4 cut(s) 305, 353, 354, 504
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 5 cut(s) 82, 352, 353, 503, 729
BsaWI WCCGGW 2 cut(s) 322, 403
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 353, 354
Bse118I RCCGGY 4 cut(s) 172, 227, 248, 416
Bse1I ACTGG 4 cut(s) 438, 1037, 1087, 1133
Bse21I CCTNAGG 1 cut(s) 974
Bse3DI GCAATG 1 cut(s) 848
BseAI TCCGGA 2 cut(s) 322, 403
BseBI CCWGG 1 cut(s) 731
BseDI CCNNGG 5 cut(s) 82, 352, 353, 503, 729
BseGI GGATG 3 cut(s) 567, 889, 943
BseLI CCNNNNNNNGG 3 cut(s) 329, 353, 354
BseMI GCAATG 1 cut(s) 848
BseMII CTCAG 5 cut(s) 401, 479, 509, 684, 828
BseNI ACTGG 4 cut(s) 438, 1037, 1087, 1133
BseRI GAGGAG 7 cut(s) 106, 109, 127, 563, 710, 751, 1130
BseXI GCAGC 4 cut(s) 567, 691, 866, 1042
BsgI GTGCAG 1 cut(s) 641
Bsh1236I CGCG 4 cut(s) 35, 47, 84, 203
BshFI GGCC 7 cut(s) 60, 176, 231, 252, 972, 979, 1071
BsiHKAI GWGCWC 2 cut(s) 322, 836
BsiHKCI CYCGRG 1 cut(s) 352
BslFI GGGAC 3 cut(s) 112, 330, 335
BslI CCNNNNNNNGG 3 cut(s) 329, 353, 354
BsmFI GGGAC 3 cut(s) 112, 330, 335
BsnI GGCC 7 cut(s) 60, 176, 231, 252, 972, 979, 1071
BsoBI CYCGRG 1 cut(s) 352
Bsp119I TTCGAA 2 cut(s) 293, 395
Bsp1286I GDGCHC 2 cut(s) 322, 836
Bsp13I TCCGGA 2 cut(s) 322, 403
Bsp143I GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
BspANI GGCC 7 cut(s) 60, 176, 231, 252, 972, 979, 1071
BspCNI CTCAG 5 cut(s) 400, 478, 510, 685, 829
BspEI TCCGGA 2 cut(s) 322, 403
BspFNI CGCG 4 cut(s) 35, 47, 84, 203
BspLI GGNNCC 2 cut(s) 346, 501
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 1 cut(s) 1021
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 2 cut(s) 293, 395
BsrDI GCAATG 1 cut(s) 848
BsrFI RCCGGY 4 cut(s) 172, 227, 248, 416
BsrI ACTGG 4 cut(s) 438, 1037, 1087, 1133
BssAI RCCGGY 4 cut(s) 172, 227, 248, 416
BssECI CCNNGG 5 cut(s) 82, 352, 353, 503, 729
BssMI GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 3 cut(s) 410, 487, 1138
Bst6I CTCTTC 3 cut(s) 17, 711, 822
BstACI GRCGYC 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 293, 395
BstC8I GCNNGC 3 cut(s) 174, 859, 981
BstDEI CTNAG 6 cut(s) 387, 465, 518, 693, 837, 974
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 3 cut(s) 567, 889, 943
BstFNI CGCG 4 cut(s) 35, 47, 84, 203
BstKTI GATC 6 cut(s) 199, 517, 567, 781, 965, 1016
BstMBI GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
BstMWI GCNNNNNNNGC 2 cut(s) 81, 339
BstNI CCWGG 1 cut(s) 731
BstNSI RCATGY 2 cut(s) 457, 479
BstSCI CCNGG 5 cut(s) 303, 351, 352, 502, 729
BstSFI CTRYAG 2 cut(s) 765, 1137
BstUI CGCG 4 cut(s) 35, 47, 84, 203
BstV1I GCAGC 4 cut(s) 567, 691, 866, 1042
BstV2I GAAGAC 2 cut(s) 746, 915
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
Bsu36I CCTNAGG 1 cut(s) 974
BsuRI GGCC 7 cut(s) 60, 176, 231, 252, 972, 979, 1071
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 567, 889, 943
BtsIMutI CAGTG 3 cut(s) 431, 483, 834
Cac8I GCNNGC 3 cut(s) 174, 859, 981
Cfr10I RCCGGY 4 cut(s) 172, 227, 248, 416
Cfr13I GGNCC 5 cut(s) 175, 344, 414, 436, 846
Cfr42I CCGCGG 1 cut(s) 85
Cfr9I CCCGGG 1 cut(s) 352
CseI GACGC 4 cut(s) 36, 41, 53, 76
Csp6I GTAC 3 cut(s) 650, 873, 1086
CviAII CATG 4 cut(s) 443, 454, 476, 905
CviQI GTAC 3 cut(s) 650, 873, 1086
DdeI CTNAG 6 cut(s) 387, 465, 518, 693, 837, 974
DpnI GATC 6 cut(s) 198, 516, 566, 780, 964, 1015
DpnII GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
DraIII CACNNNGTG 1 cut(s) 1010
EaeI YGGCCR 2 cut(s) 58, 1069
Eam1104I CTCTTC 3 cut(s) 17, 711, 822
EarI CTCTTC 3 cut(s) 17, 711, 822
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 320
Eco147I AGGCCT 1 cut(s) 972
Eco24I GRGCYC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 916
Eco47I GGWCC 4 cut(s) 344, 414, 436, 846
Eco53kI GAGCTC 1 cut(s) 320
Eco81I CCTNAGG 1 cut(s) 974
Eco88I CYCGRG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 1 cut(s) 729
EcoRV GATATC 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 322
FaeI CATG 4 cut(s) 446, 457, 479, 908
FaiI YATR 5 cut(s) 428, 444, 455, 477, 906
FaqI GGGAC 3 cut(s) 112, 330, 335
FatI CATG 4 cut(s) 442, 453, 475, 904
FauI CCCGC 2 cut(s) 80, 206
Fnu4HI GCNGC 9 cut(s) 85, 110, 334, 337, 340, 581, 680, 855, 1056
FokI GGATG 3 cut(s) 574, 896, 950
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 9 cut(s) 85, 110, 334, 337, 340, 581, 680, 855, 1056
FspBI CTAG 2 cut(s) 960, 1008
GluI GCNGC 9 cut(s) 85, 110, 334, 337, 340, 581, 680, 855, 1056
HaeIII GGCC 7 cut(s) 60, 176, 231, 252, 972, 979, 1071
HgaI GACGC 4 cut(s) 36, 41, 53, 76
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 446, 457, 479, 908
HincII GTYRAC 2 cut(s) 621, 702
HindII GTYRAC 2 cut(s) 621, 702
HindIII AAGCTT 2 cut(s) 398, 662
HinfI GANTC 9 cut(s) 143, 186, 276, 370, 522, 698, 816, 1103, 1118
HphI GGTGA 4 cut(s) 7, 61, 996, 1124
Hpy166II GTNNAC 3 cut(s) 621, 702, 1004
Hpy188III TCNNGA 2 cut(s) 323, 404
Hpy8I GTNNAC 3 cut(s) 621, 702, 1004
HpyAV CCTTC 4 cut(s) 106, 140, 323, 1103
HpyCH4III ACNGT 3 cut(s) 410, 487, 1138
HpyCH4IV ACGT 1 cut(s) 990
HpyCH4V TGCA 4 cut(s) 534, 599, 658, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 339
HpyF3I CTNAG 6 cut(s) 387, 465, 518, 693, 837, 974
HpySE526I ACGT 1 cut(s) 990
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 446, 457, 479, 908
Kpn2I TCCGGA 2 cut(s) 322, 403
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 6 cut(s) 109, 216, 306, 317, 325, 550
Lsp1109I GCAGC 4 cut(s) 567, 691, 866, 1042
MaeI CTAG 2 cut(s) 960, 1008
MaeII ACGT 1 cut(s) 990
MalI GATC 6 cut(s) 198, 516, 566, 780, 964, 1015
MboI GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
MboII GAAGA 6 cut(s) 4, 338, 698, 751, 839, 920
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 2 cut(s) 322, 836
MlsI TGGCCA 1 cut(s) 1071
MluCI AATT 5 cut(s) 206, 284, 494, 554, 755
MluI ACGCGT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 1071
MlyI GAGTC 4 cut(s) 195, 531, 707, 1127
MmeI TCCRAC 2 cut(s) 49, 392
Mox20I TGGCCA 1 cut(s) 1071
MroI TCCGGA 2 cut(s) 322, 403
MroNI GCCGGC 1 cut(s) 172
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 2 cut(s) 222, 812
MslI CAYNNNNRTG 1 cut(s) 1026
Msp20I TGGCCA 1 cut(s) 1071
MspA1I CMGCKG 2 cut(s) 84, 803
MspR9I CCNGG 5 cut(s) 305, 353, 354, 504, 731
MunI CAATTG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 731
MvnI CGCG 4 cut(s) 35, 47, 84, 203
MwoI GCNNNNNNNGC 2 cut(s) 81, 339
NaeI GCCGGC 1 cut(s) 174
NciI CCSGG 4 cut(s) 305, 353, 354, 504
NdeII GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
NgoMIV GCCGGC 1 cut(s) 172
NlaIII CATG 4 cut(s) 446, 457, 479, 908
NlaIV GGNNCC 2 cut(s) 346, 501
NmeAIII GCCGAG 2 cut(s) 36, 354
NspI RCATGY 2 cut(s) 457, 479
NspV TTCGAA 2 cut(s) 293, 395
PceI AGGCCT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 164
PdiI GCCGGC 1 cut(s) 174
PfeI GAWTC 5 cut(s) 143, 276, 370, 816, 1103
PkrI GCNGC 9 cut(s) 86, 111, 335, 338, 341, 582, 681, 856, 1057
PleI GAGTC 4 cut(s) 194, 530, 706, 1126
PpsI GAGTC 4 cut(s) 194, 530, 706, 1126
Psp124BI GAGCTC 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 729
PspGI CCWGG 1 cut(s) 729
PspN4I GGNNCC 2 cut(s) 346, 501
PspPI GGNCC 5 cut(s) 175, 344, 414, 436, 846
PstI CTGCAG 1 cut(s) 769
PsuI RGATCY 1 cut(s) 778
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 3 cut(s) 651, 874, 1087
RsaNI GTAC 3 cut(s) 650, 873, 1086
RseI CAYNNNNRTG 1 cut(s) 1026
SacI GAGCTC 1 cut(s) 322
SacII CCGCGG 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 222, 812
SatI GCNGC 9 cut(s) 85, 110, 334, 337, 340, 581, 680, 855, 1056
Sau3AI GATC 6 cut(s) 196, 514, 564, 778, 962, 1013
Sau96I GGNCC 5 cut(s) 175, 344, 414, 436, 846
SchI GAGTC 4 cut(s) 195, 531, 707, 1127
ScrFI CCNGG 5 cut(s) 305, 353, 354, 504, 731
SduI GDGCHC 2 cut(s) 322, 836
SfcI CTRYAG 2 cut(s) 765, 1137
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 293, 395
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 4 cut(s) 344, 414, 436, 846
SmaI CCCGGG 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 1026
Sse9I AATT 5 cut(s) 206, 284, 494, 554, 755
SseBI AGGCCT 1 cut(s) 972
SspMI CTAG 2 cut(s) 960, 1008
SstI GAGCTC 1 cut(s) 322
StuI AGGCCT 1 cut(s) 972
StyD4I CCNGG 5 cut(s) 303, 351, 352, 502, 729
TaaI ACNGT 3 cut(s) 410, 487, 1138
TaiI ACGT 1 cut(s) 993
TaqI TCGA 7 cut(s) 293, 395, 489, 539, 819, 1091, 1121
TasI AATT 5 cut(s) 206, 284, 494, 554, 755
TatI WGTACW 1 cut(s) 649
TauI GCSGC 5 cut(s) 87, 112, 336, 339, 342
TfiI GAWTC 5 cut(s) 143, 276, 370, 816, 1103
Tru1I TTAA 2 cut(s) 222, 812
Tru9I TTAA 2 cut(s) 222, 812
TscAI CASTG 3 cut(s) 438, 490, 841
TseI GCWGC 4 cut(s) 580, 679, 854, 1055
TspDTI ATGAA 3 cut(s) 698, 840, 921
TspGWI ACGGA 2 cut(s) 256, 330
TspMI CCCGGG 1 cut(s) 352
TspRI CASTG 3 cut(s) 438, 490, 841
VpaK11BI GGWCC 4 cut(s) 344, 414, 436, 846
XapI RAATTY 1 cut(s) 755
XceI RCATGY 2 cut(s) 457, 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XmaI CCCGGG 1 cut(s) 352
XspI CTAG 2 cut(s) 960, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.