Rmu_sc0003153.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003153.1
OG0009740
Physical Location & Seq
Forward (+)
38733 .. 39690
958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003153.1_g000010.1.cds

Sequence Viewer

Length: 684 bp
atgcgacatcatatggacaaggcacttagattggagtatatgtcaatcaaggatgcaagagagctatgggtagcgctagaagagcattttggcaatgtccaagattccctcctccctgacttgaaggttcaatggaacaatctgcgctttgctgatttcaagtatgttgctgaatataattcagaagctctgcgcgtacagtccatgttgaggttttgtggacaacctgtcacagagcaagagctaattgagaaaactctctccaccttccctgtttcagccattgtggtatcaaaacaataccatactgaagtcaatgctggacggatcggaaggaaaccacagtttggagtggatacacgtggtggcaatgccacacgtaggagagggggtcgtggcaaccctagccgtggtggtggagtcatagctcgtggtggtggcgccatgggtcgtggtggtggcaccatggatcgtggtggtggagccaaccatcctagggaacgaccacaacttgcacctcaattgaagggaggcaaccacaatgatatggcatatagggacctgagggagcaagaagtgtaccttgcagaagaagaagaagaaaatggtggagacgttaatctcaccatagaggacttcaaagctgaagatgaagtgcacaaggatgccgcagactttgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

227

Amino Acids

25.37

Weight (kDa)

5.86

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 440, 461
AccB7I CCANNNNNTGG 1 cut(s) 347
AccII CGCG 1 cut(s) 195
AciI CCGC 1 cut(s) 669
AclWI GGATC 2 cut(s) 335, 477
AcuI CTGAAG 2 cut(s) 330, 666
AcvI CACGTG 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 441
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 2 cut(s) 198, 581
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 5 cut(s) 210, 347, 381, 410, 496
AflIII ACRYGT 2 cut(s) 359, 377
AgsI TTSAA 5 cut(s) 124, 131, 160, 526, 640
AhdI GACNNNNNGTC 1 cut(s) 227
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 5 cut(s) 64, 188, 244, 428, 644
AluI AGCT 5 cut(s) 64, 188, 244, 428, 644
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 656
AlwI GGATC 2 cut(s) 335, 477
Aor51HI AGCGCT 1 cut(s) 75
ApaLI GTGCAC 1 cut(s) 656
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 4 cut(s) 76, 147, 195, 443
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 559
AvrII CCTAGG 1 cut(s) 494
AxyI CCTNAGG 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 660
BanI GGYRCC 2 cut(s) 440, 461
BauI CACGAG 1 cut(s) 429
BbrPI CACGTG 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 660
BccI CCATC 1 cut(s) 498
BceAI ACGGC 1 cut(s) 393
BciVI GTATCC 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 3 cut(s) 77, 405, 495
BfoI RGCGCY 2 cut(s) 77, 444
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 1 cut(s) 669
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 1 cut(s) 670
Bme18I GGWCC 1 cut(s) 559
BmeRI GACNNNNNGTC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 4 cut(s) 442, 463, 484, 560
BmsI GCATC 2 cut(s) 43, 655
BsaAI YACGTR 2 cut(s) 362, 380
BsaHI GRCGYC 1 cut(s) 441
BsaJI CCNNGG 4 cut(s) 409, 444, 465, 494
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 376, 406
Bsc4I CCNNNNNNNGG 5 cut(s) 210, 347, 381, 410, 496
Bse21I CCTNAGG 1 cut(s) 563
Bse3DI GCAATG 2 cut(s) 100, 376
BseDI CCNNGG 4 cut(s) 409, 444, 465, 494
BseGI GGATG 3 cut(s) 58, 490, 670
BseLI CCNNNNNNNGG 5 cut(s) 210, 347, 381, 410, 496
BseMI GCAATG 2 cut(s) 100, 376
BseMII CTCAG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 660
Bsh1236I CGCG 1 cut(s) 195
BshNI GGYRCC 2 cut(s) 440, 461
BsiHKAI GWGCWC 1 cut(s) 660
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 5 cut(s) 210, 347, 381, 410, 496
BsmAI GTCTC 1 cut(s) 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 572
Bso31I GGTCTC 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 2 cut(s) 327, 469
Bsp19I CCATGG 2 cut(s) 444, 465
BspACI CCGC 1 cut(s) 669
BspCNI CTCAG 1 cut(s) 555
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 4 cut(s) 442, 463, 484, 560
BspPI GGATC 2 cut(s) 335, 477
BspQI GCTCTTC 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 440, 461
BsrDI GCAATG 2 cut(s) 100, 376
BssECI CCNNGG 4 cut(s) 409, 444, 465, 494
BssMI GATC 2 cut(s) 327, 469
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 3 cut(s) 444, 465, 494
Bst2BI CACGAG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 201, 345
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 441
BstBAI YACGTR 2 cut(s) 362, 380
BstDEI CTNAG 2 cut(s) 26, 563
BstDSI CCRYGG 3 cut(s) 409, 444, 465
BstF5I GGATG 3 cut(s) 58, 490, 670
BstFNI CGCG 1 cut(s) 195
BstH2I RGCGCY 2 cut(s) 77, 444
BstHHI GCGC 4 cut(s) 76, 147, 195, 443
BstKTI GATC 2 cut(s) 330, 472
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 2 cut(s) 327, 469
BstMWI GCNNNNNNNGC 2 cut(s) 82, 405
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 1 cut(s) 195
Bsu36I CCTNAGG 1 cut(s) 563
BsuI GTATCC 1 cut(s) 349
BtgI CCRYGG 3 cut(s) 409, 444, 465
BtsCI GGATG 3 cut(s) 58, 490, 670
CfoI GCGC 4 cut(s) 76, 147, 195, 443
Cfr13I GGNCC 1 cut(s) 559
Csp6I GTAC 2 cut(s) 197, 580
CviAII CATG 3 cut(s) 205, 445, 466
CviJI RGCY 8 cut(s) 64, 188, 244, 281, 408, 428, 485, 644
CviKI_1 RGCY 8 cut(s) 64, 188, 244, 281, 408, 428, 485, 644
CviQI GTAC 2 cut(s) 197, 580
DdeI CTNAG 2 cut(s) 26, 563
DinI GGCGCC 1 cut(s) 442
DpnI GATC 2 cut(s) 329, 471
DpnII GATC 2 cut(s) 327, 469
DraIII CACNNNGTG 1 cut(s) 365
DriI GACNNNNNGTC 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 75
Eam1105I GACNNNNNGTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 3 cut(s) 444, 465, 494
Eco47I GGWCC 1 cut(s) 559
Eco47III AGCGCT 1 cut(s) 75
Eco57I CTGAAG 2 cut(s) 330, 666
Eco72I CACGTG 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 559
EcoT14I CCWWGG 3 cut(s) 444, 465, 494
EgeI GGCGCC 1 cut(s) 442
EheI GGCGCC 1 cut(s) 442
ErhI CCWWGG 3 cut(s) 444, 465, 494
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 3 cut(s) 208, 448, 469
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FaqI GGGAC 1 cut(s) 572
FatI CATG 3 cut(s) 204, 444, 465
FauNDI CATATG 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 669
FokI GGATG 3 cut(s) 65, 477, 677
Fsp4HI GCNGC 1 cut(s) 669
FspBI CTAG 3 cut(s) 77, 405, 495
GlaI GCGC 4 cut(s) 75, 146, 194, 442
GluI GCNGC 1 cut(s) 669
HaeII RGCGCY 2 cut(s) 77, 444
HhaI GCGC 4 cut(s) 76, 147, 195, 443
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 3 cut(s) 208, 448, 469
Hin6I GCGC 4 cut(s) 74, 145, 193, 441
HinP1I GCGC 4 cut(s) 74, 145, 193, 441
HinfI GANTC 2 cut(s) 104, 420
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 3 cut(s) 221, 580, 658
Hpy188I TCNGA 2 cut(s) 184, 332
Hpy8I GTNNAC 3 cut(s) 221, 580, 658
HpyAV CCTTC 4 cut(s) 118, 277, 327, 520
HpyCH4III ACNGT 2 cut(s) 201, 345
HpyCH4IV ACGT 3 cut(s) 361, 379, 615
HpyCH4V TGCA 4 cut(s) 56, 515, 587, 658
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 405
HpyF3I CTNAG 2 cut(s) 26, 563
HpySE526I ACGT 3 cut(s) 361, 379, 615
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 3 cut(s) 208, 448, 469
HspAI GCGC 4 cut(s) 74, 145, 193, 441
KasI GGCGCC 1 cut(s) 440
Kzo9I GATC 2 cut(s) 327, 469
LguI GCTCTTC 1 cut(s) 75
LmnI GCTCC 2 cut(s) 482, 568
LpnPI CCDG 5 cut(s) 129, 240, 285, 306, 575
LweI GCATC 2 cut(s) 43, 655
MaeI CTAG 3 cut(s) 77, 405, 495
MaeII ACGT 3 cut(s) 361, 379, 615
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 329, 471
MboI GATC 2 cut(s) 327, 469
MboII GAAGA 6 cut(s) 92, 602, 605, 608, 611, 659
MfeI CAATTG 1 cut(s) 521
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 3 cut(s) 178, 246, 521
Mly113I GGCGCC 1 cut(s) 441
MlyI GAGTC 1 cut(s) 429
MnlI CCTC 8 cut(s) 119, 122, 204, 380, 524, 528, 558, 625
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 663
MunI CAATTG 1 cut(s) 521
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 2 cut(s) 82, 405
NarI GGCGCC 1 cut(s) 441
NcoI CCATGG 2 cut(s) 444, 465
NdeI CATATG 1 cut(s) 12
NdeII GATC 2 cut(s) 327, 469
NlaIII CATG 3 cut(s) 208, 448, 469
NlaIV GGNNCC 4 cut(s) 442, 463, 484, 560
NmuCI GTSAC 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 104
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 1 cut(s) 670
PleI GAGTC 1 cut(s) 428
PluTI GGCGCC 1 cut(s) 444
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 2 cut(s) 362, 380
PpuMI RGGWCCY 1 cut(s) 559
Psp5II RGGWCCY 1 cut(s) 559
PspCI CACGTG 1 cut(s) 362
PspN4I GGNNCC 4 cut(s) 442, 463, 484, 560
PspPI GGNCC 1 cut(s) 559
PspPPI RGGWCCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 198, 581
RsaNI GTAC 2 cut(s) 197, 580
RseI CAYNNNNRTG 1 cut(s) 663
SapI GCTCTTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 1 cut(s) 669
Sau3AI GATC 2 cut(s) 327, 469
Sau96I GGNCC 1 cut(s) 559
SchI GAGTC 1 cut(s) 429
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 2 cut(s) 43, 655
SfoI GGCGCC 1 cut(s) 442
SinI GGWCC 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 663
Sse9I AATT 3 cut(s) 178, 246, 521
SsiI CCGC 1 cut(s) 669
SspDI GGCGCC 1 cut(s) 440
SspMI CTAG 3 cut(s) 77, 405, 495
StyI CCWWGG 3 cut(s) 444, 465, 494
TaaI ACNGT 2 cut(s) 201, 345
TaiI ACGT 3 cut(s) 364, 382, 618
TasI AATT 3 cut(s) 178, 246, 521
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 666
TspGWI ACGGA 1 cut(s) 340
Van91I CCANNNNNTGG 1 cut(s) 347
VneI GTGCAC 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 559
XmaJI CCTAGG 1 cut(s) 494
XspI CTAG 3 cut(s) 77, 405, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.