Rh2AG120600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009740
Physical Location & Seq
Reverse (-)
10848349 .. 10907606
59258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG120600.1

Sequence Viewer

Length: 294 bp
ATGGCATATGGATCGGGTCCTTGGTCCGCCCAGCTCTTGGGATTGCAGCGTCGCTATTATTTCTGGTCTCGATCGGTTGAATTCGAAATTGGATTTGTCATCGCCGTTGACAACTACAGTGGAGTAGAGGTCGAATTTCGGCGGGATTTTTCAGTGGATGGTGCCGGTCGGATCCTTTTCTGTACATGCAGAGCCGCCGAGCACCAAGCTCTAGATCCGACGGCAGTCCACGGCTTCAAAGAATTGAGTAGATGCAAATGTTATATTATTTTATTGTTGTATGGTGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

11.1

Weight (kDa)

6.06

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 37
AciI CCGC 3 cut(s) 27, 142, 195
AclWI GGATC 4 cut(s) 19, 166, 179, 209
AcsI RAATTY 2 cut(s) 80, 134
AfaI GTAC 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 80, 238
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 2 cut(s) 34, 209
AluI AGCT 2 cut(s) 34, 209
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 4 cut(s) 19, 166, 179, 209
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 2 cut(s) 80, 134
AspS9I GGNCC 2 cut(s) 17, 24
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 2 cut(s) 17, 24
BamHI GGATCC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 58
BccI CCATC 1 cut(s) 152
BceAI ACGGC 3 cut(s) 89, 237, 247
BcgI CGANNNNNNTGC 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 2 cut(s) 47, 195
BlsI GCNGC 2 cut(s) 48, 196
Bme18I GGWCC 2 cut(s) 17, 24
BmgT120I GGNCC 2 cut(s) 17, 24
BmiI GGNNCC 3 cut(s) 18, 163, 173
BmsI GCATC 1 cut(s) 242
BoxI GACNNNNGTC 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 20, 229
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 20, 229
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 37
BseXI GCAGC 1 cut(s) 58
BseYI CCCAGC 1 cut(s) 30
Bsh1285I CGRYCG 2 cut(s) 74, 169
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 74, 169
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 182
Bsp143I GATC 4 cut(s) 11, 71, 171, 214
BspACI CCGC 3 cut(s) 27, 142, 195
BspLI GGNNCC 3 cut(s) 18, 163, 173
BspPI GGATC 4 cut(s) 19, 166, 179, 209
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 20, 229
BssMI GATC 4 cut(s) 11, 71, 171, 214
BssT1I CCWWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 119
BstAUI TGTACA 1 cut(s) 182
BstBI TTCGAA 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 4 cut(s) 14, 74, 174, 217
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 4 cut(s) 11, 71, 171, 214
BstMCI CGRYCG 2 cut(s) 74, 169
BstNSI RCATGY 1 cut(s) 189
BstPAI GACNNNNGTC 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 2 cut(s) 171, 214
BstYI RGATCY 2 cut(s) 171, 214
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 2 cut(s) 124, 159
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 17, 24
CseI GACGC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 183
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 1 cut(s) 186
CviJI RGCY 4 cut(s) 34, 194, 209, 234
CviKI_1 RGCY 4 cut(s) 34, 194, 209, 234
CviQI GTAC 1 cut(s) 183
DpnI GATC 4 cut(s) 13, 73, 173, 216
DpnII GATC 4 cut(s) 11, 71, 171, 214
EciI GGCGGA 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 17, 24
EcoO109I RGGNCCY 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 1 cut(s) 189
FaiI YATR 5 cut(s) 7, 9, 187, 264, 282
FatI CATG 1 cut(s) 185
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 7
Fnu4HI GCNGC 2 cut(s) 47, 195
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 47, 195
FspBI CTAG 1 cut(s) 212
GluI GCNGC 2 cut(s) 47, 195
GsaI CCCAGC 1 cut(s) 34
HapII CCGG 1 cut(s) 165
HgaI GACGC 1 cut(s) 38
Hin1II CATG 1 cut(s) 189
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HpaII CCGG 1 cut(s) 165
Hpy166II GTNNAC 2 cut(s) 109, 229
Hpy188I TCNGA 2 cut(s) 171, 219
Hpy188III TCNNGA 2 cut(s) 69, 212
Hpy8I GTNNAC 2 cut(s) 109, 229
Hpy99I CGWCG 2 cut(s) 54, 223
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 46, 189, 255
Hsp92II CATG 1 cut(s) 189
Kzo9I GATC 4 cut(s) 11, 71, 171, 214
LpnPI CCDG 3 cut(s) 44, 49, 178
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 1 cut(s) 242
MaeI CTAG 1 cut(s) 212
MalI GATC 4 cut(s) 13, 73, 173, 216
MboI GATC 4 cut(s) 11, 71, 171, 214
MflI RGATCY 2 cut(s) 171, 214
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 4 cut(s) 80, 87, 134, 242
MmeI TCCRAC 2 cut(s) 149, 242
MnlI CCTC 1 cut(s) 121
MspI CCGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 7
NdeII GATC 4 cut(s) 11, 71, 171, 214
NlaIII CATG 1 cut(s) 189
NlaIV GGNNCC 3 cut(s) 18, 163, 173
NmeAIII GCCGAG 1 cut(s) 223
NspI RCATGY 1 cut(s) 189
NspV TTCGAA 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 2 cut(s) 48, 196
Ple19I CGATCG 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 17
PshAI GACNNNNGTC 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 17
PspFI CCCAGC 1 cut(s) 30
PspN4I GGNNCC 3 cut(s) 18, 163, 173
PspPI GGNCC 2 cut(s) 17, 24
PspPPI RGGWCCY 1 cut(s) 17
PsuI RGATCY 2 cut(s) 171, 214
PvuI CGATCG 1 cut(s) 74
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SatI GCNGC 2 cut(s) 47, 195
Sau3AI GATC 4 cut(s) 11, 71, 171, 214
Sau96I GGNCC 2 cut(s) 17, 24
SduI GDGCHC 1 cut(s) 204
SetI ASST 3 cut(s) 36, 132, 211
SfaNI GCATC 1 cut(s) 242
SfcI CTRYAG 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 2 cut(s) 17, 24
Sse9I AATT 4 cut(s) 80, 87, 134, 242
SsiI CCGC 3 cut(s) 27, 142, 195
SspMI CTAG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 119
TaqI TCGA 3 cut(s) 70, 84, 132
TasI AATT 4 cut(s) 80, 87, 134, 242
TatI WGTACW 1 cut(s) 182
TauI GCSGC 1 cut(s) 197
TscAI CASTG 2 cut(s) 124, 159
TseI GCWGC 1 cut(s) 46
TspRI CASTG 2 cut(s) 124, 159
Van91I CCANNNNNTGG 1 cut(s) 37
VpaK11BI GGWCC 2 cut(s) 17, 24
XapI RAATTY 2 cut(s) 80, 134
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 189
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.