Rmu_sc0005220.1_g000004

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005220.1
OG0008302
Physical Location & Seq
Reverse (-)
22970 .. 23482
513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
atgaacagctggttaaccccaactgtcctcttcctcctcctccagctcatgattggcatcatcttcattgcttccagcctcggcacccagaagccccacaaccaagacaaccaccaccaacaaggcttggcgagatctccctttgttttgcagcggttcaaatccataaactactactcctacagatctctggaaccggccacgaattatgaaaaatcccctgaatccgagacccattttgctttcccgcacgcccaggaggttgaacagcccaagctccccagatctccttccatgctggagcggctccagtccttcaagttccagttccacttccccaaagaatccagcccgctacagccccgagacttcgtagtcccggacccggaaccgaaaccccatttcagtacttacgagcactctcatgaagacgatgaagagtcggaagaggaggccggagagttggatgaggaccaattcgaagccgaagaagaaggtgaagagaaagactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

170

Amino Acids

19.83

Weight (kDa)

4.76

Isoelectric Point (pI)

68.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 1 - 27 2e-07 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 304
AciI CCGC 4 cut(s) 154, 248, 304, 353
AcoI YGGCCR 1 cut(s) 198
AfaI GTAC 1 cut(s) 409
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 3 cut(s) 160, 266, 319
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 3 cut(s) 9, 46, 277
AluI AGCT 3 cut(s) 9, 46, 277
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 2 cut(s) 224, 360
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 198, 453
ApeKI GCWGC 1 cut(s) 151
AspS9I GGNCC 2 cut(s) 382, 472
AsuC2I CCSGG 2 cut(s) 380, 386
AsuHPI GGTGA 1 cut(s) 509
AsuII TTCGAA 1 cut(s) 480
AvaI CYCGRG 1 cut(s) 363
AvaII GGWCC 2 cut(s) 382, 472
BanI GGYRCC 1 cut(s) 83
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 257
BcnI CCSGG 2 cut(s) 380, 386
BcoDI GTCTC 2 cut(s) 224, 360
BfaI CTAG 1 cut(s) 511
BfmI CTRYAG 2 cut(s) 181, 356
BglII AGATCT 3 cut(s) 134, 185, 284
BisI GCNGC 2 cut(s) 152, 305
BlsI GCNGC 2 cut(s) 153, 306
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 3 cut(s) 257, 380, 386
Bme18I GGWCC 2 cut(s) 382, 472
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 382, 472
BmiI GGNNCC 5 cut(s) 85, 195, 308, 384, 390
BmrFI CCNGG 3 cut(s) 257, 380, 386
BmsI GCATC 1 cut(s) 66
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 3 cut(s) 26, 293, 320
Bpu14I TTCGAA 1 cut(s) 480
BpuMI CCSGG 2 cut(s) 380, 386
BsaBI GATNNNNATC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 79, 255
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 2 cut(s) 310, 325
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 56
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 2 cut(s) 79, 255
BseGI GGATG 1 cut(s) 472
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 66
BseNI ACTGG 2 cut(s) 310, 325
BseRI GAGGAG 3 cut(s) 26, 29, 464
BseXI GCAGC 1 cut(s) 163
BshFI GGCC 2 cut(s) 200, 455
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 4 cut(s) 197, 380, 386, 456
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 2 cut(s) 224, 360
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 200, 455
Bso31I GGTCTC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 3 cut(s) 134, 185, 284
BspACI CCGC 4 cut(s) 154, 248, 304, 353
BspANI GGCC 2 cut(s) 200, 455
BspHI TCATGA 2 cut(s) 48, 424
BspLI GGNNCC 5 cut(s) 85, 195, 308, 384, 390
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 2 cut(s) 310, 325
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 79, 255
BssMI GATC 3 cut(s) 134, 185, 284
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 4 cut(s) 35, 432, 441, 495
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 252, 353
BstF5I GGATG 1 cut(s) 472
BstKTI GATC 3 cut(s) 137, 188, 287
BstMAI GTCTC 2 cut(s) 224, 360
BstMBI GATC 3 cut(s) 134, 185, 284
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 3 cut(s) 255, 378, 384
BstSFI CTRYAG 2 cut(s) 181, 356
BstV1I GCAGC 1 cut(s) 163
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 3 cut(s) 134, 185, 284
BstYI RGATCY 3 cut(s) 134, 185, 284
BsuRI GGCC 2 cut(s) 200, 455
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 252, 353
CciI TCATGA 2 cut(s) 48, 424
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 382, 472
Csp6I GTAC 1 cut(s) 408
CviAII CATG 3 cut(s) 49, 295, 425
CviQI GTAC 1 cut(s) 408
DpnI GATC 3 cut(s) 136, 187, 286
DpnII GATC 3 cut(s) 134, 185, 284
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 4 cut(s) 35, 432, 441, 495
EarI CTCTTC 4 cut(s) 35, 432, 441, 495
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 382, 472
Eco88I CYCGRG 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 255
FaeI CATG 3 cut(s) 52, 298, 428
FaiI YATR 5 cut(s) 50, 167, 210, 296, 426
FaqI GGGAC 1 cut(s) 362
FatI CATG 3 cut(s) 48, 294, 424
FauI CCCGC 2 cut(s) 255, 360
Fnu4HI GCNGC 2 cut(s) 152, 305
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 2 cut(s) 152, 305
FspBI CTAG 1 cut(s) 511
GluI GCNGC 2 cut(s) 152, 305
GsuI CTGGAG 3 cut(s) 26, 293, 320
HaeIII GGCC 2 cut(s) 200, 455
HapII CCGG 4 cut(s) 197, 380, 386, 456
Hin1II CATG 3 cut(s) 52, 298, 428
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 3 cut(s) 224, 344, 440
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 4 cut(s) 197, 380, 386, 456
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 2 cut(s) 229, 445
Hpy188III TCNNGA 3 cut(s) 49, 191, 425
Hpy8I GTNNAC 1 cut(s) 15
HpyAV CCTTC 3 cut(s) 300, 325, 488
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
Hsp92II CATG 3 cut(s) 52, 298, 428
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 3 cut(s) 134, 185, 284
LmnI GCTCC 3 cut(s) 282, 301, 312
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 66
MaeI CTAG 1 cut(s) 511
MalI GATC 3 cut(s) 136, 187, 286
MbiI CCGCTC 1 cut(s) 304
MboI GATC 3 cut(s) 134, 185, 284
MboII GAAGA 8 cut(s) 22, 55, 440, 449, 458, 500, 503, 512
MflI RGATCY 3 cut(s) 134, 185, 284
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 2 cut(s) 205, 476
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 423, 444
MnlI CCTC 9 cut(s) 38, 44, 47, 50, 89, 253, 442, 445, 463
MseI TTAA 1 cut(s) 14
MslI CAYNNNNRTG 1 cut(s) 423
MspA1I CMGCKG 2 cut(s) 9, 154
MspI CCGG 4 cut(s) 197, 380, 386, 456
MspR9I CCNGG 3 cut(s) 257, 380, 386
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 304
NciI CCSGG 2 cut(s) 380, 386
NdeII GATC 3 cut(s) 134, 185, 284
NlaIII CATG 3 cut(s) 52, 298, 428
NlaIV GGNNCC 5 cut(s) 85, 195, 308, 384, 390
NmeAIII GCCGAG 1 cut(s) 60
NspV TTCGAA 1 cut(s) 480
PagI TCATGA 2 cut(s) 48, 424
PfeI GAWTC 2 cut(s) 224, 344
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 153, 306
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 5 cut(s) 85, 195, 308, 384, 390
PspPI GGNCC 2 cut(s) 382, 472
PsuI RGATCY 3 cut(s) 134, 185, 284
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
RseI CAYNNNNRTG 1 cut(s) 423
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 152, 305
Sau3AI GATC 3 cut(s) 134, 185, 284
Sau96I GGNCC 2 cut(s) 382, 472
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 257, 380, 386
SduI GDGCHC 1 cut(s) 420
SetI ASST 5 cut(s) 11, 48, 264, 279, 499
SfaNI GCATC 1 cut(s) 66
SfcI CTRYAG 2 cut(s) 181, 356
SfuI TTCGAA 1 cut(s) 480
SinI GGWCC 2 cut(s) 382, 472
SmiMI CAYNNNNRTG 1 cut(s) 423
Sse9I AATT 2 cut(s) 205, 476
SsiI CCGC 4 cut(s) 154, 248, 304, 353
SspMI CTAG 1 cut(s) 511
StyD4I CCNGG 3 cut(s) 255, 378, 384
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 480
TasI AATT 2 cut(s) 205, 476
TatI WGTACW 1 cut(s) 407
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 2 cut(s) 224, 344
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TseI GCWGC 1 cut(s) 151
TspDTI ATGAA 5 cut(s) 17, 55, 225, 441, 450
VpaK11BI GGWCC 2 cut(s) 382, 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XspI CTAG 1 cut(s) 511
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.