Rmu_sc0005706.1_g000007

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005706.1
OG0008185
Physical Location & Seq
Forward (+)
24813 .. 28299
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
atggggattctgtggattgattcaaccaacttgcggacgcttaaatatttcatgatgttggttgacacgcaaacacgctggatcactcctcggatcatcctattcagtcaattggatttgactgtgctagagaatttacatcgaaagactttcgatggatattgcaatgagactgatgttggacatcatattcccatgtacacacctaaatggtcttgtggaaacgactacgatggtagtccggacattggaacgataggaattgtcgtggcttgttccccactatgtctcatctcgttccctgttaaagtgacgagatcacacaaatatgccgcaaacaagcctgcatggaaggacgtccctacgagaggacgtagcgccaccgtacacggaggtaggcacgacaccaaagccaaagagagtggcactctaatgttacagaccatggccccagctaggatgcatgggattcccgtgggttcgaaggatgctttagcacactccaatcctttgatcatcaacactcaaaatccatctaatgagaatcttccgggttatggcgatagggttgcttggaggactgatgaagaatttgccagagaaatgctaactggagtcaaccctgtaaccatttgtcgcctccaaacgtttccaccagcaagcaaactagatccaaatgtttatggtgatcacagtagtaccataacagaacaccacatcaagaataacttggatgggctaacggtgcatcaggtctccgattaa

Protein Analysis

254

Amino Acids

28.3

Weight (kDa)

7.2

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipoxygenase PF00305 188 - 251 6.9e-23 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 2 cut(s) 34, 333
AclI AACGTT 1 cut(s) 647
AclWI GGATC 3 cut(s) 89, 101, 665
AcsI RAATTY 2 cut(s) 133, 590
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 200, 387, 700
AfiI CCNNNNNNNGG 5 cut(s) 33, 248, 368, 456, 557
AgsI TTSAA 1 cut(s) 24
AluBI AGCT 1 cut(s) 455
AluI AGCT 1 cut(s) 455
Alw26I GTCTC 3 cut(s) 164, 293, 760
AlwI GGATC 3 cut(s) 89, 101, 665
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 1 cut(s) 447
ApoI RAATTY 2 cut(s) 133, 590
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 552
AsuHPI GGTGA 1 cut(s) 698
AsuII TTCGAA 1 cut(s) 482
BaeI ACNNNNGTAYC 2 cut(s) 682, 715
BccI CCATC 4 cut(s) 149, 227, 541, 728
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BclI TGATCA 2 cut(s) 513, 688
BcnI CCSGG 1 cut(s) 552
BcoDI GTCTC 3 cut(s) 164, 293, 760
BfaI CTAG 3 cut(s) 128, 456, 668
BfoI RGCGCY 1 cut(s) 381
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bme1390I CCNGG 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 1 cut(s) 552
BmsI GCATC 3 cut(s) 450, 478, 757
BplI GAGNNNNNCTC 2 cut(s) 412, 444
BpmI CTGGAG 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 760
BsaJI CCNNGG 3 cut(s) 89, 444, 474
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 248, 368, 456, 557
Bse1I ACTGG 1 cut(s) 616
Bse3DI GCAATG 1 cut(s) 172
BseAI TCCGGA 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 89, 444, 474
BseGI GGATG 4 cut(s) 96, 465, 493, 739
BseLI CCNNNNNNNGG 5 cut(s) 33, 248, 368, 456, 557
BseMI GCAATG 1 cut(s) 172
BseNI ACTGG 1 cut(s) 616
BseRI GAGGAG 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 1 cut(s) 449
BsiSI CCGG 2 cut(s) 242, 551
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 5 cut(s) 33, 248, 368, 456, 557
BsmAI GTCTC 3 cut(s) 164, 293, 760
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 1 cut(s) 449
Bso31I GGTCTC 1 cut(s) 760
Bsp119I TTCGAA 1 cut(s) 482
Bsp13I TCCGGA 1 cut(s) 241
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 6 cut(s) 81, 93, 317, 513, 670, 688
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 2 cut(s) 34, 333
BspANI GGCC 1 cut(s) 449
BspEI TCCGGA 1 cut(s) 241
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 450
BspPI GGATC 3 cut(s) 89, 101, 665
BspT104I TTCGAA 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 760
BsrDI GCAATG 1 cut(s) 172
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 3 cut(s) 89, 444, 474
BssMI GATC 6 cut(s) 81, 93, 317, 513, 670, 688
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 444
Bst4CI ACNGT 4 cut(s) 124, 385, 695, 745
BstACI GRCGYC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 2 cut(s) 345, 661
BstDSI CCRYGG 2 cut(s) 444, 474
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 4 cut(s) 96, 465, 493, 739
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 6 cut(s) 84, 96, 320, 516, 673, 691
BstMAI GTCTC 3 cut(s) 164, 293, 760
BstMBI GATC 6 cut(s) 81, 93, 317, 513, 670, 688
BstMWI GCNNNNNNNGC 1 cut(s) 745
BstSCI CCNGG 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 1 cut(s) 449
BtgI CCRYGG 2 cut(s) 444, 474
BtsCI GGATG 4 cut(s) 96, 465, 493, 739
Cac8I GCNNGC 2 cut(s) 345, 661
CciI TCATGA 1 cut(s) 51
CfoI GCGC 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 448
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 199, 386, 699
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 5 cut(s) 52, 196, 348, 445, 464
CviJI RGCY 6 cut(s) 272, 343, 413, 449, 455, 739
CviKI_1 RGCY 6 cut(s) 272, 343, 413, 449, 455, 739
CviQI GTAC 3 cut(s) 199, 386, 699
DpnI GATC 6 cut(s) 83, 95, 319, 515, 672, 690
DpnII GATC 6 cut(s) 81, 93, 317, 513, 670, 688
Eco130I CCWWGG 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 5 cut(s) 55, 199, 351, 448, 467
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 1 cut(s) 344
FatI CATG 5 cut(s) 51, 195, 347, 444, 463
FbaI TGATCA 2 cut(s) 513, 688
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 4 cut(s) 83, 472, 500, 746
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 3 cut(s) 128, 456, 668
GlaI GCGC 1 cut(s) 379
GluI GCNGC 1 cut(s) 333
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 1 cut(s) 449
HapII CCGG 2 cut(s) 242, 551
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 380
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 5 cut(s) 55, 199, 351, 448, 467
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HincII GTYRAC 2 cut(s) 64, 619
HindII GTYRAC 2 cut(s) 64, 619
HinfI GANTC 5 cut(s) 7, 20, 469, 544, 615
HpaII CCGG 2 cut(s) 242, 551
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 4 cut(s) 64, 201, 388, 619
Hpy188I TCNGA 2 cut(s) 93, 760
Hpy188III TCNNGA 3 cut(s) 52, 242, 721
Hpy8I GTNNAC 4 cut(s) 64, 201, 388, 619
HpyAV CCTTC 2 cut(s) 346, 478
HpyCH4III ACNGT 4 cut(s) 124, 385, 695, 745
HpyCH4IV ACGT 3 cut(s) 357, 373, 647
HpyCH4V TGCA 4 cut(s) 165, 347, 463, 748
HpyF10VI GCNNNNNNNGC 1 cut(s) 745
HpySE526I ACGT 3 cut(s) 357, 373, 647
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 5 cut(s) 55, 199, 351, 448, 467
HspAI GCGC 1 cut(s) 378
Kpn2I TCCGGA 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 513, 688
Kzo9I GATC 6 cut(s) 81, 93, 317, 513, 670, 688
LweI GCATC 3 cut(s) 450, 478, 757
MaeI CTAG 3 cut(s) 128, 456, 668
MaeII ACGT 3 cut(s) 357, 373, 647
MaeIII GTNAC 3 cut(s) 310, 435, 625
MalI GATC 6 cut(s) 83, 95, 319, 515, 672, 690
MboI GATC 6 cut(s) 81, 93, 317, 513, 670, 688
MboII GAAGA 2 cut(s) 539, 599
MfeI CAATTG 1 cut(s) 110
MflI RGATCY 1 cut(s) 670
MluCI AATT 4 cut(s) 110, 133, 261, 590
MlyI GAGTC 1 cut(s) 624
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 5 cut(s) 99, 362, 386, 570, 650
Mph1103I ATGCAT 1 cut(s) 465
MroI TCCGGA 1 cut(s) 241
MseI TTAA 3 cut(s) 42, 306, 763
MslI CAYNNNNRTG 3 cut(s) 208, 327, 431
MspI CCGG 2 cut(s) 242, 551
MspR9I CCNGG 1 cut(s) 552
MunI CAATTG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 745
NciI CCSGG 1 cut(s) 552
NcoI CCATGG 1 cut(s) 444
NdeII GATC 6 cut(s) 81, 93, 317, 513, 670, 688
NlaIII CATG 5 cut(s) 55, 199, 351, 448, 467
NlaIV GGNNCC 1 cut(s) 450
NmuCI GTSAC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 465
NspV TTCGAA 1 cut(s) 482
PagI TCATGA 1 cut(s) 51
PfeI GAWTC 4 cut(s) 7, 20, 469, 544
PkrI GCNGC 1 cut(s) 334
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Psp1406I AACGTT 1 cut(s) 647
PspFI CCCAGC 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 1 cut(s) 448
PsuI RGATCY 1 cut(s) 670
RsaI GTAC 3 cut(s) 200, 387, 700
RsaNI GTAC 3 cut(s) 199, 386, 699
RseI CAYNNNNRTG 3 cut(s) 208, 327, 431
SaqAI TTAA 3 cut(s) 42, 306, 763
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 6 cut(s) 81, 93, 317, 513, 670, 688
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 1 cut(s) 624
ScrFI CCNGG 1 cut(s) 552
SetI ASST 7 cut(s) 208, 360, 376, 397, 457, 650, 756
SfaNI GCATC 3 cut(s) 450, 478, 757
SfuI TTCGAA 1 cut(s) 482
SmiMI CAYNNNNRTG 3 cut(s) 208, 327, 431
Sse9I AATT 4 cut(s) 110, 133, 261, 590
SsiI CCGC 2 cut(s) 34, 333
SspI AATATT 1 cut(s) 47
SspMI CTAG 3 cut(s) 128, 456, 668
StyD4I CCNGG 1 cut(s) 550
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 4 cut(s) 124, 385, 695, 745
TaiI ACGT 3 cut(s) 360, 376, 650
TaqI TCGA 3 cut(s) 142, 153, 482
TasI AATT 4 cut(s) 110, 133, 261, 590
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 4 cut(s) 7, 20, 469, 544
Tru1I TTAA 3 cut(s) 42, 306, 763
Tru9I TTAA 3 cut(s) 42, 306, 763
TseFI GTSAC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 40, 600
TspGWI ACGGA 1 cut(s) 405
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 2 cut(s) 133, 590
XspI CTAG 3 cut(s) 128, 456, 668
ZraI GACGTC 1 cut(s) 358
Zsp2I ATGCAT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.