Rmu_sc0006414.1_g000014

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006414.1
OG0007557
Physical Location & Seq
Forward (+)
51031 .. 51552
522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
atgatacttccggtgagcatccgctgcaacacttgtgccaatcgcatgccccaaggcaccactttgaactatgtaagacgacgcccccccctctccgagacttcaatagttacatttttcttcaattgtaccaagtgccgggcactcatccagatgaaggaagacccagagaattcaggccatgttgttgaggatggtgctacagaagttcttgaacatgagaaatcagaacaccttggaaagattcatgatgaagatggtttggatcatgaaacatctgactctaatttgaagaaatcagaacaccttggaaagattcgtgatgaagatggtttggatcctgaaacatctgactctaatttgaagaaatctgggcatcatcgaaagattcatgatgaagctggcttagattatgaaacatccgactctgatttgaagagacaaaagcttatgattgggcttcctagcaacccaacagagtcgttcactctttcggatagctcacgcacagaggtatgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

173

Amino Acids

19.45

Weight (kDa)

5.34

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 1 - 102 2.3e-13 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 1 cut(s) 22
AclWI GGATC 3 cut(s) 273, 332, 345
AcsI RAATTY 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 82
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 138, 157
AgsI TTSAA 7 cut(s) 67, 105, 124, 215, 292, 364, 436
AluBI AGCT 3 cut(s) 401, 448, 501
AluI AGCT 3 cut(s) 401, 448, 501
Alw26I GTCTC 2 cut(s) 92, 433
AlwI GGATC 3 cut(s) 273, 332, 345
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 1 cut(s) 172
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 11
BccI CCATC 3 cut(s) 188, 251, 323
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 92, 433
BfaI CTAG 2 cut(s) 465, 520
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 58, 339
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 27, 385
BpiI GAAGAC 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 52, 235, 307
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 157
BseDI CCNNGG 3 cut(s) 52, 235, 307
BseGI GGATG 4 cut(s) 18, 147, 199, 419
BseLI CCNNNNNNNGG 2 cut(s) 138, 157
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 11, 139
BslI CCNNNNNNNGG 2 cut(s) 138, 157
BsmAI GTCTC 2 cut(s) 92, 433
BsnI GGCC 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 145
Bsp143I GATC 2 cut(s) 265, 337
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 180
BspHI TCATGA 3 cut(s) 247, 268, 391
BspLI GGNNCC 2 cut(s) 58, 339
BspPI GGATC 3 cut(s) 273, 332, 345
BspT107I GGYRCC 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 52, 235, 307
BssMI GATC 2 cut(s) 265, 337
BssNI GRCGYC 1 cut(s) 82
BssT1I CCWWGG 3 cut(s) 52, 235, 307
Bst6I CTCTTC 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 47, 403
BstDEI CTNAG 1 cut(s) 406
BstF5I GGATG 4 cut(s) 18, 147, 199, 419
BstKTI GATC 2 cut(s) 268, 340
BstMAI GTCTC 2 cut(s) 92, 433
BstMBI GATC 2 cut(s) 265, 337
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 11
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 4 cut(s) 18, 147, 199, 419
Cac8I GCNNGC 2 cut(s) 47, 403
CciI TCATGA 3 cut(s) 247, 268, 391
CseI GACGC 1 cut(s) 90
Csp6I GTAC 1 cut(s) 129
CviAII CATG 6 cut(s) 46, 182, 218, 248, 269, 392
CviJI RGCY 6 cut(s) 180, 401, 405, 448, 460, 501
CviKI_1 RGCY 6 cut(s) 180, 401, 405, 448, 460, 501
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 1 cut(s) 406
DpnI GATC 2 cut(s) 267, 339
DpnII GATC 2 cut(s) 265, 337
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 52, 235, 307
EcoRI GAATTC 1 cut(s) 172
EcoT14I CCWWGG 3 cut(s) 52, 235, 307
ErhI CCWWGG 3 cut(s) 52, 235, 307
FaeI CATG 6 cut(s) 49, 185, 221, 251, 272, 395
FatI CATG 6 cut(s) 45, 181, 217, 247, 268, 391
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 4 cut(s) 5, 134, 206, 406
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 2 cut(s) 465, 520
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 1 cut(s) 180
HapII CCGG 2 cut(s) 11, 139
HgaI GACGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 82
Hin1II CATG 6 cut(s) 49, 185, 221, 251, 272, 395
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 7 cut(s) 244, 281, 316, 353, 388, 425, 479
HpaII CCGG 2 cut(s) 11, 139
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 486
Hpy188I TCNGA 8 cut(s) 97, 229, 280, 301, 352, 424, 430, 496
Hpy188III TCNNGA 7 cut(s) 151, 212, 248, 269, 320, 341, 392
Hpy8I GTNNAC 1 cut(s) 486
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 151
HpyCH4V TGCA 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 82
Hsp92II CATG 6 cut(s) 49, 185, 221, 251, 272, 395
Kzo9I GATC 2 cut(s) 265, 337
LpnPI CCDG 8 cut(s) 24, 152, 162, 164, 180, 354, 357, 387
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 2 cut(s) 27, 385
MaeI CTAG 2 cut(s) 465, 520
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 267, 339
MboI GATC 2 cut(s) 265, 337
MboII GAAGA 7 cut(s) 112, 173, 266, 304, 338, 376, 448
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 4 cut(s) 124, 172, 286, 358
MlyI GAGTC 4 cut(s) 275, 347, 419, 488
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 3 cut(s) 101, 184, 505
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 2 cut(s) 11, 139
MspR9I CCNGG 1 cut(s) 140
MunI CAATTG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 1 cut(s) 140
NdeII GATC 2 cut(s) 265, 337
NlaIII CATG 6 cut(s) 49, 185, 221, 251, 272, 395
NlaIV GGNNCC 2 cut(s) 58, 339
NspI RCATGY 1 cut(s) 49
PaeI GCATGC 1 cut(s) 49
PagI TCATGA 3 cut(s) 247, 268, 391
PfeI GAWTC 3 cut(s) 244, 316, 388
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 4 cut(s) 275, 347, 419, 487
PpsI GAGTC 4 cut(s) 275, 347, 419, 487
PspN4I GGNNCC 2 cut(s) 58, 339
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 1 cut(s) 152
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 2 cut(s) 265, 337
SchI GAGTC 4 cut(s) 275, 347, 419, 488
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 145
SetI ASST 6 cut(s) 237, 309, 403, 450, 503, 516
SfaNI GCATC 2 cut(s) 27, 385
SfcI CTRYAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 152
SphI GCATGC 1 cut(s) 49
Sse9I AATT 4 cut(s) 124, 172, 286, 358
SsiI CCGC 1 cut(s) 22
SspMI CTAG 2 cut(s) 465, 520
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 3 cut(s) 52, 235, 307
TaqI TCGA 1 cut(s) 382
TasI AATT 4 cut(s) 124, 172, 286, 358
TfiI GAWTC 3 cut(s) 244, 316, 388
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 8 cut(s) 170, 236, 267, 285, 339, 380, 411, 429
XapI RAATTY 1 cut(s) 172
XceI RCATGY 1 cut(s) 49
XspI CTAG 2 cut(s) 465, 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.