Rmu_ssc0000303.1_g000015

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000303.1
OG0002763
Physical Location & Seq
Forward (+)
77427 .. 83984
6558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2571 bp
atggtggtaccaagccaaagggttctcttgatatcttttctacttttgttggtctcgaccacattaatattccatgccaaaggtgacaagccgaaaccagaaggtggtggcgtggctgctggggacggtggggatggcggtgacggagaggttgccggggacactacagatgacggggcagtacagggaggaaagaagcagcttgcacacggtcccgtgacgtatgatgccaggtctttgatcattaatgggaaacatgagcttctattttctggttcaattcattatccacgtagcccggtggaaatgtggcctgatattctgaagaaggctaaacaaggaggtttgaacgtgattcaaacttatgtgttttggaacattcatgagcctgtgcaaggcaagtgggagttcgagggaaactatgatttggtgaagtttattaggttgatccaggagaatggaatgtatgctaccctcagggttggaccattcatccaagcagaatggaaccatggaggtcttccatattggctgagagaggtcccaggaatcactttccgatcagataatgaaccatacaagaaacacatggagcaatatgtgaacaagataatgaagatgatgaaggatgaaaagctattttctccacaaggaggtccaatcatcttagctcagattgagaatgagtacaatcaaatccaacttgcatatagagagttgggagatacatatgtgcagtgggcagcaaagctggcagttgggcagaatattggagtgccatggatcatgtgcaagcaaaaggatgctcctgatccagtgattaatacttgcaacggaaggcattgtggagatactttttcaggacctaacaaacccaacaagcctgctctatggactgagaattggacagctcagtaccgagtatttggtgaccctccttcacaaagagcagcagaagacattgcattttcagttgctcggtttttctccaagggtggggctctaaccaactactatatgtaccatggtggaacgaattttggaagaacaagtgctgttttcacaacaactcgttactacgatgaggctcctctcgatgaatatgctttgcaaagggacccgaaatggagtcacctcaaggacttgcacaaggctataaatctatgcaagaaaactttgcttactggaactcccggagaccaaaggttgggagaagagactgaggttcgtttctatgagaagccagggacagagtgtgctgctttcatagcaaataatcactcgaccatggaagcaaccgttaattggaggggacagaagtttctcctgccaccagcttccattagcatcctccccgactgcaagactgtggtcttcaatactcaacaaattgtatcccaacataattcaaggaactttgttacagctagtaaaaaaatgaactgggaaatgtctcccgaacccattgcaaccgtcgcgcaaatcccatccaatagtagggaaatgcaggagctctatctcttgctaaaagataccacagactatgcatggtacacaactagcattgacctaagtccgtatgacttgccgatgaaggagactatcaggccagttgtacgaattcctagtcttggtcatgcaatggctgtatttgtcaatgacgaatatattggatcgcaacacggtagccacgcagagaaaggtttcgtgttcgaggcacctgctaaaatgaagctaggggcgaaccatatctctctgctttgcattgtagtgggactcccagatagtggagcgtacatggagcacaggtatgcagggcctcgttctgtaacaatcctaggattgaacaccggaacaatcgacctgtccgcaaatggctggggacatagggctggcctgaatggtgaaaagctcgaagtgtacactgagaagggagctcagaaagtgaaatggactaaggcaacaggtaaaggaagggctctcacatggtacaagacaagttttgatgctcctccaggaagagacccttgtgctattcgaatgaccggtatgggcaagggaatggtatgggtgaatggtataagcatcggccgtcattggatgtccttcctctctcctcttggacaacctactcaaatcgagtaccacatcccaagagccttcattaagcctaagaatttccttgttgtattggaggaggagccagcagacccaaaagacattgagatcgtaacagtcaacagagacaccatctgcagccttattaaacaggactatccgggacatgttaagtactgggatagaaaaggcggcaagctcacaacagctcctggaaaaagtctccaaccatcagctctcctcaactgtccaaactacaaaaagatcagtgctattgaattcgcgggctatggcaatcctgacggcttctgtggagcctttaaattgggacaatgcaatgctcctgaagccaagaaggtcgtcgaagatgaaagtaatgcagtccaagcttatgaatcggagcagatagcacccatgaaggtccttggatcagcgcagcccccattgaactga

Protein Analysis

856

Amino Acids

95.05

Weight (kDa)

8.05

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 79 - 385 2.6e-116 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 100 - 232 4.2e-06 Beta-galactosidase
GHD PF17834 409 - 464 4.2e-20 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 661 - 725 8.8e-22 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1729
Acc36I ACCTGC 1 cut(s) 1729
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 2 cut(s) 7, 1717
AccB7I CCANNNNNTGG 3 cut(s) 104, 1207, 1787
AccII CGCG 2 cut(s) 1478, 2402
AciI CCGC 4 cut(s) 138, 1869, 2310, 2402
AclWI GGATC 5 cut(s) 442, 791, 806, 1681, 2554
AcoI YGGCCR 1 cut(s) 2089
AcsI RAATTY 4 cut(s) 1036, 1620, 2176, 2396
AcuI CTGAAG 2 cut(s) 344, 2484
AfiI CCNNNNNNNGG 9 cut(s) 104, 395, 653, 996, 1207, 1305, 1497, 1631, 1787
AflIII ACRYGT 1 cut(s) 2284
AgeI ACCGGT 1 cut(s) 2045
AgsI TTSAA 8 cut(s) 279, 349, 359, 1378, 1410, 1846, 2396, 2566
AjnI CCWGG 6 cut(s) 230, 450, 544, 1243, 2014, 2329
Alw21I GWGCWC 3 cut(s) 1515, 1806, 1939
Alw26I GTCTC 8 cut(s) 58, 1191, 1211, 1458, 1592, 2016, 2238, 2345
AlwI GGATC 5 cut(s) 442, 791, 806, 1681, 2554
AlwNI CAGNNNCTG 1 cut(s) 2330
AoxI GGCC 5 cut(s) 311, 1607, 1817, 1894, 2089
ApeKI GCWGC 7 cut(s) 116, 199, 743, 950, 1259, 2256, 2554
ApoI RAATTY 4 cut(s) 1036, 1620, 2176, 2396
ArsI GACNNNNNNTTYG 2 cut(s) 1612, 1644
AseI ATTAAT 3 cut(s) 65, 246, 822
AsiGI ACCGGT 1 cut(s) 2045
Asp700I GAANNNNTTC 1 cut(s) 1703
Asp718I GGTACC 1 cut(s) 7
AspA2I CCTAGG 1 cut(s) 1837
AspLEI GCGC 2 cut(s) 1480, 2554
AspS9I GGNCC 8 cut(s) 212, 485, 541, 656, 863, 1117, 1817, 2539
AsuC2I CCSGG 4 cut(s) 157, 299, 1194, 2280
AsuHPI GGTGA 7 cut(s) 95, 152, 442, 941, 1124, 1916, 2083
AsuII TTCGAA 1 cut(s) 2038
AvaII GGWCC 7 cut(s) 212, 485, 541, 656, 863, 1117, 2539
AvrII CCTAGG 1 cut(s) 1837
AxyI CCTNAGG 1 cut(s) 476
BaeI ACNNNNGTAYC 2 cut(s) 717, 750
BanI GGYRCC 2 cut(s) 7, 1717
BanII GRGCYC 4 cut(s) 1003, 1515, 1939, 1981
BbsI GAAGAC 3 cut(s) 512, 963, 1366
Bbv12I GWGCWC 3 cut(s) 1515, 1806, 1939
BbvI GCAGC 7 cut(s) 103, 211, 755, 962, 1246, 2268, 2566
BccI CCATC 4 cut(s) 128, 1495, 2258, 2356
BceAI ACGGC 2 cut(s) 2076, 2437
BcgI CGANNNNNNTGC 6 cut(s) 1085, 1119, 1448, 1482, 1703, 1737
BciT130I CCWGG 6 cut(s) 232, 452, 546, 1245, 2016, 2331
BciVI GTATCC 1 cut(s) 1405
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 4 cut(s) 157, 299, 1194, 2280
BcoDI GTCTC 8 cut(s) 58, 1191, 1211, 1458, 1592, 2016, 2238, 2345
BfaI CTAG 5 cut(s) 1428, 1560, 1626, 1736, 1838
BfmI CTRYAG 2 cut(s) 165, 2254
BfuAI ACCTGC 1 cut(s) 1729
BfuI GTATCC 1 cut(s) 1405
BisI GCNGC 8 cut(s) 117, 200, 744, 951, 1260, 2257, 2311, 2555
BlnI CCTAGG 1 cut(s) 1837
BlsI GCNGC 8 cut(s) 118, 201, 745, 952, 1261, 2258, 2312, 2556
BmcAI AGTACT 1 cut(s) 2294
Bme18I GGWCC 7 cut(s) 212, 485, 541, 656, 863, 1117, 2539
BmgT120I GGNCC 8 cut(s) 212, 485, 541, 656, 863, 1117, 1817, 2539
BmrI ACTGGG 2 cut(s) 1453, 2305
BmsI GCATC 5 cut(s) 217, 793, 1356, 1996, 2094
BmuI ACTGGG 2 cut(s) 1453, 2305
BoxI GACNNNNGTC 2 cut(s) 1370, 1572
BpiI GAAGAC 3 cut(s) 512, 963, 1366
BpmI CTGGAG 1 cut(s) 1998
Bpu14I TTCGAA 1 cut(s) 2038
BpuEI CTTGAG 1 cut(s) 1121
BpuMI CCSGG 4 cut(s) 157, 299, 1194, 2280
BsaAI YACGTR 1 cut(s) 293
BsaI GGTCTC 3 cut(s) 58, 1191, 2016
BsaWI WCCGGW 2 cut(s) 1850, 2045
BsaXI ACNNNNNCTCC 6 cut(s) 1174, 1204, 1793, 1823, 2311, 2341
Bsc4I CCNNNNNNNGG 9 cut(s) 104, 395, 653, 996, 1207, 1305, 1497, 1631, 1787
Bse118I RCCGGY 1 cut(s) 2045
Bse1I ACTGG 5 cut(s) 815, 1189, 1448, 1610, 2300
Bse21I CCTNAGG 1 cut(s) 476
Bse3DI GCAATG 4 cut(s) 960, 1464, 1647, 2461
BseBI CCWGG 6 cut(s) 232, 452, 546, 1245, 2016, 2331
BseGI GGATG 8 cut(s) 139, 492, 634, 808, 1347, 1487, 2106, 2148
BseLI CCNNNNNNNGG 9 cut(s) 104, 395, 653, 996, 1207, 1305, 1497, 1631, 1787
BseMI GCAATG 4 cut(s) 960, 1464, 1647, 2461
BseMII CTCAG 8 cut(s) 490, 524, 686, 888, 926, 1212, 1917, 1952
BseNI ACTGG 5 cut(s) 815, 1189, 1448, 1610, 2300
BseRI GAGGAG 6 cut(s) 1080, 2001, 2106, 2210, 2213, 2348
BseX3I CGGCCG 1 cut(s) 2089
BseXI GCAGC 7 cut(s) 103, 211, 755, 962, 1246, 2268, 2566
BseYI CCCAGC 2 cut(s) 119, 1878
BsgI GTGCAG 1 cut(s) 755
Bsh1236I CGCG 2 cut(s) 1478, 2402
Bsh1285I CGRYCG 1 cut(s) 2092
BshFI GGCC 5 cut(s) 313, 1609, 1819, 1896, 2091
BshNI GGYRCC 2 cut(s) 7, 1717
BshTI ACCGGT 1 cut(s) 2045
BsiEI CGRYCG 1 cut(s) 2092
BsiHKAI GWGCWC 3 cut(s) 1515, 1806, 1939
BsiSI CCGG 6 cut(s) 156, 299, 1194, 1851, 2046, 2279
BslI CCNNNNNNNGG 9 cut(s) 104, 395, 653, 996, 1207, 1305, 1497, 1631, 1787
BsmAI GTCTC 8 cut(s) 58, 1191, 1211, 1458, 1592, 2016, 2238, 2345
BsnI GGCC 5 cut(s) 313, 1609, 1819, 1896, 2091
Bso31I GGTCTC 3 cut(s) 58, 1191, 2016
Bsp119I TTCGAA 1 cut(s) 2038
Bsp1286I GDGCHC 5 cut(s) 1003, 1515, 1806, 1939, 1981
Bsp1407I TGTACA 1 cut(s) 1920
Bsp143I GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
Bsp19I CCATGG 4 cut(s) 511, 779, 1024, 1287
BspACI CCGC 4 cut(s) 138, 1869, 2310, 2402
BspANI GGCC 5 cut(s) 313, 1609, 1819, 1896, 2091
BspCNI CTCAG 8 cut(s) 489, 525, 685, 889, 925, 1213, 1918, 1951
BspFNI CGCG 2 cut(s) 1478, 2402
BspHI TCATGA 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 2258
BspMI ACCTGC 1 cut(s) 1729
BspPI GGATC 5 cut(s) 442, 791, 806, 1681, 2554
BspT104I TTCGAA 1 cut(s) 2038
BspT107I GGYRCC 2 cut(s) 7, 1717
BspTNI GGTCTC 3 cut(s) 58, 1191, 2016
BsrDI GCAATG 4 cut(s) 960, 1464, 1647, 2461
BsrFI RCCGGY 1 cut(s) 2045
BsrGI TGTACA 1 cut(s) 1920
BsrI ACTGG 5 cut(s) 815, 1189, 1448, 1610, 2300
BssAI RCCGGY 1 cut(s) 2045
BssMI GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
BssT1I CCWWGG 7 cut(s) 511, 779, 990, 1024, 1287, 1837, 2542
Bst2UI CCWGG 6 cut(s) 232, 452, 546, 1245, 2016, 2331
Bst4CI ACNGT 8 cut(s) 128, 212, 1300, 1369, 1474, 1685, 2236, 2366
Bst6I CTCTTC 2 cut(s) 1209, 2014
BstAUI TGTACA 1 cut(s) 1920
BstBAI YACGTR 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 2038
BstC8I GCNNGC 8 cut(s) 204, 753, 794, 885, 1894, 2205, 2315, 2404
BstDSI CCRYGG 4 cut(s) 511, 779, 1024, 1287
BstEII GGTNACC 1 cut(s) 929
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 8 cut(s) 139, 492, 634, 808, 1347, 1487, 2106, 2148
BstFNI CGCG 2 cut(s) 1478, 2402
BstHHI GCGC 2 cut(s) 1480, 2554
BstKTI GATC 9 cut(s) 243, 450, 563, 786, 814, 1676, 2229, 2385, 2549
BstMAI GTCTC 8 cut(s) 58, 1191, 1211, 1458, 1592, 2016, 2238, 2345
BstMBI GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
BstMCI CGRYCG 1 cut(s) 2092
BstMWI GCNNNNNNNGC 5 cut(s) 752, 1268, 1475, 2465, 2504
BstNI CCWGG 6 cut(s) 232, 452, 546, 1245, 2016, 2331
BstNSI RCATGY 1 cut(s) 2288
BstPAI GACNNNNGTC 2 cut(s) 1370, 1572
BstPI GGTNACC 1 cut(s) 929
BstSFI CTRYAG 2 cut(s) 165, 2254
BstUI CGCG 2 cut(s) 1478, 2402
BstV1I GCAGC 7 cut(s) 103, 211, 755, 962, 1246, 2268, 2566
BstV2I GAAGAC 3 cut(s) 512, 963, 1366
BstXI CCANNNNNNTGG 1 cut(s) 458
BstZI CGGCCG 1 cut(s) 2089
Bsu36I CCTNAGG 1 cut(s) 476
BsuI GTATCC 1 cut(s) 1405
BsuRI GGCC 5 cut(s) 313, 1609, 1819, 1896, 2091
BtgI CCRYGG 4 cut(s) 511, 779, 1024, 1287
BtsCI GGATG 8 cut(s) 139, 492, 634, 808, 1347, 1487, 2106, 2148
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 4 cut(s) 743, 822, 1923, 2392
BveI ACCTGC 1 cut(s) 1729
Cac8I GCNNGC 8 cut(s) 204, 753, 794, 885, 1894, 2205, 2315, 2404
CaiI CAGNNNCTG 1 cut(s) 2330
CciI TCATGA 1 cut(s) 382
CfoI GCGC 2 cut(s) 1480, 2554
Cfr10I RCCGGY 1 cut(s) 2045
Cfr13I GGNCC 8 cut(s) 212, 485, 541, 656, 863, 1117, 1817, 2539
CspAI ACCGGT 1 cut(s) 2045
CspCI CAANNNNNGTGG 2 cut(s) 383, 418
DpnI GATC 9 cut(s) 242, 449, 562, 785, 813, 1675, 2228, 2384, 2548
DpnII GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
DraI TTTAAA 1 cut(s) 2440
EaeI YGGCCR 1 cut(s) 2089
EagI CGGCCG 1 cut(s) 2089
Eam1104I CTCTTC 2 cut(s) 1209, 2014
EarI CTCTTC 2 cut(s) 1209, 2014
Ecl136II GAGCTC 2 cut(s) 1513, 1937
EclXI CGGCCG 1 cut(s) 2089
Eco130I CCWWGG 7 cut(s) 511, 779, 990, 1024, 1287, 1837, 2542
Eco24I GRGCYC 4 cut(s) 1003, 1515, 1939, 1981
Eco31I GGTCTC 3 cut(s) 58, 1191, 2016
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 7 cut(s) 212, 485, 541, 656, 863, 1117, 2539
Eco52I CGGCCG 1 cut(s) 2089
Eco53kI GAGCTC 2 cut(s) 1513, 1937
Eco57I CTGAAG 2 cut(s) 344, 2484
Eco81I CCTNAGG 1 cut(s) 476
Eco91I GGTNACC 1 cut(s) 929
EcoICRI GAGCTC 2 cut(s) 1513, 1937
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 5 cut(s) 541, 863, 1117, 1817, 2539
EcoO65I GGTNACC 1 cut(s) 929
EcoRI GAATTC 2 cut(s) 1620, 2396
EcoRII CCWGG 6 cut(s) 230, 450, 544, 1243, 2014, 2329
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 7 cut(s) 511, 779, 990, 1024, 1287, 1837, 2542
EcoT22I ATGCAT 1 cut(s) 1549
EcoT38I GRGCYC 4 cut(s) 1003, 1515, 1939, 1981
ErhI CCWWGG 7 cut(s) 511, 779, 990, 1024, 1287, 1837, 2542
FalI AAGNNNNNCTT 2 cut(s) 2011, 2043
FauI CCCGC 1 cut(s) 2395
FauNDI CATATG 1 cut(s) 730
FbaI TGATCA 1 cut(s) 240
Fnu4HI GCNGC 8 cut(s) 117, 200, 744, 951, 1260, 2257, 2311, 2555
FokI GGATG 8 cut(s) 146, 479, 641, 815, 1334, 1474, 2113, 2135
FriOI GRGCYC 4 cut(s) 1003, 1515, 1939, 1981
Fsp4HI GCNGC 8 cut(s) 117, 200, 744, 951, 1260, 2257, 2311, 2555
FspBI CTAG 5 cut(s) 1428, 1560, 1626, 1736, 1838
GlaI GCGC 2 cut(s) 1479, 2553
GluI GCNGC 8 cut(s) 117, 200, 744, 951, 1260, 2257, 2311, 2555
GsaI CCCAGC 2 cut(s) 123, 1882
GsuI CTGGAG 1 cut(s) 1998
HaeIII GGCC 5 cut(s) 313, 1609, 1819, 1896, 2091
HapII CCGG 6 cut(s) 156, 299, 1194, 1851, 2046, 2279
HhaI GCGC 2 cut(s) 1480, 2554
Hin6I GCGC 2 cut(s) 1478, 2552
HinP1I GCGC 2 cut(s) 1478, 2552
HincII GTYRAC 1 cut(s) 2239
HindII GTYRAC 1 cut(s) 2239
HindIII AAGCTT 1 cut(s) 2505
HinfI GANTC 5 cut(s) 355, 549, 1129, 1776, 2513
HpaII CCGG 6 cut(s) 156, 299, 1194, 1851, 2046, 2279
HphI GGTGA 7 cut(s) 95, 152, 442, 941, 1124, 1916, 2083
Hpy166II GTNNAC 5 cut(s) 604, 1554, 1921, 1923, 2239
Hpy188I TCNGA 6 cut(s) 324, 560, 565, 675, 1941, 2518
Hpy188III TCNNGA 9 cut(s) 28, 55, 383, 809, 861, 1094, 1457, 2417, 2462
Hpy8I GTNNAC 5 cut(s) 604, 1554, 1921, 1923, 2239
Hpy99I CGWCG 2 cut(s) 1478, 2483
HpyCH4III ACNGT 8 cut(s) 128, 212, 1300, 1369, 1474, 1685, 2236, 2366
HpyCH4IV ACGT 3 cut(s) 221, 292, 351
HpyF10VI GCNNNNNNNGC 5 cut(s) 752, 1268, 1475, 2465, 2504
HpySE526I ACGT 3 cut(s) 221, 292, 351
HspAI GCGC 2 cut(s) 1478, 2552
KflI GGGWCCC 1 cut(s) 1117
KpnI GGTACC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
Lsp1109I GCAGC 7 cut(s) 103, 211, 755, 962, 1246, 2268, 2566
LweI GCATC 5 cut(s) 217, 793, 1356, 1996, 2094
MaeI CTAG 5 cut(s) 1428, 1560, 1626, 1736, 1838
MaeII ACGT 3 cut(s) 221, 292, 351
MaeIII GTNAC 9 cut(s) 83, 140, 217, 929, 1073, 1130, 1420, 1828, 2230
MalI GATC 9 cut(s) 242, 449, 562, 785, 813, 1675, 2228, 2384, 2548
MboI GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
MboII GAAGA 9 cut(s) 337, 512, 628, 968, 1056, 1226, 1366, 2031, 2495
MhlI GDGCHC 5 cut(s) 1003, 1515, 1806, 1939, 1981
MlyI GAGTC 2 cut(s) 1138, 1770
MmeI TCCRAC 3 cut(s) 463, 724, 2368
Mph1103I ATGCAT 1 cut(s) 1549
MroXI GAANNNNTTC 1 cut(s) 1703
MseI TTAA 8 cut(s) 65, 246, 822, 1302, 2166, 2265, 2289, 2439
MslI CAYNNNNRTG 2 cut(s) 1769, 1809
MspI CCGG 6 cut(s) 156, 299, 1194, 1851, 2046, 2279
MvaI CCWGG 6 cut(s) 232, 452, 546, 1245, 2016, 2331
MvnI CGCG 2 cut(s) 1478, 2402
MwoI GCNNNNNNNGC 5 cut(s) 752, 1268, 1475, 2465, 2504
NciI CCSGG 4 cut(s) 157, 299, 1194, 2280
NcoI CCATGG 4 cut(s) 511, 779, 1024, 1287
NdeI CATATG 1 cut(s) 730
NdeII GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
NmuCI GTSAC 5 cut(s) 83, 140, 217, 929, 1130
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 1 cut(s) 2288
NspV TTCGAA 1 cut(s) 2038
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 1729
PciI ACATGT 1 cut(s) 2284
PdmI GAANNNNTTC 1 cut(s) 1703
PfeI GAWTC 3 cut(s) 355, 549, 2513
PflMI CCANNNNNTGG 3 cut(s) 104, 1207, 1787
PfoI TCCNGGA 5 cut(s) 450, 1192, 2014, 2278, 2329
PinAI ACCGGT 1 cut(s) 2045
PkrI GCNGC 8 cut(s) 118, 201, 745, 952, 1261, 2258, 2312, 2556
PleI GAGTC 2 cut(s) 1137, 1770
PpsI GAGTC 2 cut(s) 1137, 1770
Ppu21I YACGTR 1 cut(s) 293
PpuMI RGGWCCY 4 cut(s) 541, 863, 1117, 2539
PscI ACATGT 1 cut(s) 2284
PshAI GACNNNNGTC 2 cut(s) 1370, 1572
PshBI ATTAAT 3 cut(s) 65, 246, 822
Psp124BI GAGCTC 2 cut(s) 1515, 1939
Psp5II RGGWCCY 4 cut(s) 541, 863, 1117, 2539
Psp6I CCWGG 6 cut(s) 230, 450, 544, 1243, 2014, 2329
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 2 cut(s) 119, 1878
PspGI CCWGG 6 cut(s) 230, 450, 544, 1243, 2014, 2329
PspPI GGNCC 8 cut(s) 212, 485, 541, 656, 863, 1117, 1817, 2539
PspPPI RGGWCCY 4 cut(s) 541, 863, 1117, 2539
PstI CTGCAG 1 cut(s) 2258
PstNI CAGNNNCTG 1 cut(s) 2330
RseI CAYNNNNRTG 2 cut(s) 1769, 1809
SacI GAGCTC 2 cut(s) 1515, 1939
SaqAI TTAA 8 cut(s) 65, 246, 822, 1302, 2166, 2265, 2289, 2439
SatI GCNGC 8 cut(s) 117, 200, 744, 951, 1260, 2257, 2311, 2555
Sau3AI GATC 9 cut(s) 240, 447, 560, 783, 811, 1673, 2226, 2382, 2546
Sau96I GGNCC 8 cut(s) 212, 485, 541, 656, 863, 1117, 1817, 2539
ScaI AGTACT 1 cut(s) 2294
SchI GAGTC 2 cut(s) 1138, 1770
SduI GDGCHC 5 cut(s) 1003, 1515, 1806, 1939, 1981
SfaNI GCATC 5 cut(s) 217, 793, 1356, 1996, 2094
SfcI CTRYAG 2 cut(s) 165, 2254
SfuI TTCGAA 1 cut(s) 2038
SinI GGWCC 7 cut(s) 212, 485, 541, 656, 863, 1117, 2539
SmiMI CAYNNNNRTG 2 cut(s) 1769, 1809
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
SsiI CCGC 4 cut(s) 138, 1869, 2310, 2402
SspI AATATT 2 cut(s) 69, 769
SspMI CTAG 5 cut(s) 1428, 1560, 1626, 1736, 1838
SstI GAGCTC 2 cut(s) 1515, 1939
StyI CCWWGG 7 cut(s) 511, 779, 990, 1024, 1287, 1837, 2542
TaaI ACNGT 8 cut(s) 128, 212, 1300, 1369, 1474, 1685, 2236, 2366
TaiI ACGT 3 cut(s) 224, 295, 354
TatI WGTACW 4 cut(s) 181, 687, 1920, 2292
TauI GCSGC 1 cut(s) 2313
TfiI GAWTC 3 cut(s) 355, 549, 2513
Tru1I TTAA 8 cut(s) 65, 246, 822, 1302, 2166, 2265, 2289, 2439
Tru9I TTAA 8 cut(s) 65, 246, 822, 1302, 2166, 2265, 2289, 2439
TscAI CASTG 4 cut(s) 743, 822, 1930, 2392
TseFI GTSAC 5 cut(s) 83, 140, 217, 929, 1130
TseI GCWGC 7 cut(s) 116, 199, 743, 950, 1259, 2256, 2554
Tsp45I GTSAC 5 cut(s) 83, 140, 217, 929, 1130
TspGWI ACGGA 3 cut(s) 159, 849, 1566
TspRI CASTG 4 cut(s) 743, 822, 1930, 2392
Van91I CCANNNNNTGG 3 cut(s) 104, 1207, 1787
VpaK11BI GGWCC 7 cut(s) 212, 485, 541, 656, 863, 1117, 2539
VspI ATTAAT 3 cut(s) 65, 246, 822
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 4 cut(s) 1036, 1620, 2176, 2396
XceI RCATGY 1 cut(s) 2288
XmaJI CCTAGG 1 cut(s) 1837
XmnI GAANNNNTTC 1 cut(s) 1703
XspI CTAG 5 cut(s) 1428, 1560, 1626, 1736, 1838
ZrmI AGTACT 1 cut(s) 2294
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.