Rroxscaffold_1G00063300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006937
Physical Location & Seq
Forward (+)
85195207 .. 85198454
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063300.1

Sequence Viewer

Length: 435 bp
ATGGCTGTATATTTTAGCGCGCAGTGTCTTGACATTTCTGATTGGAACATGTTTGTGTTTTCTAAACCACTTCAAGACCCCGGTTACTGTAAAATCTACTCGTCACAACACCTTCCCCGGTGGGAGGATCGAGGAGCCGCACGACATCATAATTCCTGCTTTGTGTGCCATGGTTTCGACTACACCGAACAGAATGAACCAATGCTTTTACAAGCTCTAGCAGGGCGATTCCCAGGCACTCGCACCCACGTCATTTCTCGTCGAAATCTAGTCATCAGAATTATAACTTTTAACGATGCTTGGTCTGCCATAGTAGCTTGGGAAGATACTAGAGATGGGATGGAATTGGCTATCGGACCGCAGAGGTACACACTAGAGTTCTTTGAGCCTACTCCTCAGCACATCGGGATACTACGGTATGAAGAGCAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.84

Weight (kDa)

6.04

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 138, 359
AclWI GGATC 1 cut(s) 135
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 124
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 1 cut(s) 74
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 2 cut(s) 215, 317
AluI AGCT 2 cut(s) 215, 317
AlwI GGATC 1 cut(s) 135
AspLEI GCGC 2 cut(s) 20, 22
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 2 cut(s) 81, 118
AvaII GGWCC 1 cut(s) 356
BaeI ACNNNNGTAYC 2 cut(s) 401, 434
BbvCI CCTCAGC 1 cut(s) 396
BccI CCATC 2 cut(s) 329, 334
BciT130I CCWGG 1 cut(s) 234
BciVI GTATCC 1 cut(s) 402
BcnI CCSGG 2 cut(s) 81, 118
BfaI CTAG 4 cut(s) 218, 269, 330, 374
BfuI GTATCC 1 cut(s) 402
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 3 cut(s) 81, 118, 234
Bme18I GGWCC 1 cut(s) 356
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 3 cut(s) 81, 118, 234
BmsI GCATC 1 cut(s) 286
Bpu10I CCTNAGC 1 cut(s) 396
BpuMI CCSGG 2 cut(s) 81, 118
BsaJI CCNNGG 4 cut(s) 79, 116, 169, 232
Bsc4I CCNNNNNNNGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 4 cut(s) 79, 116, 169, 232
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 410
BsePI GCGCGC 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 147, 384
Bsh1236I CGCG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 81, 118
BslI CCNNNNNNNGG 1 cut(s) 124
Bsp143I GATC 1 cut(s) 127
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 2 cut(s) 138, 359
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 136
BspPI GGATC 1 cut(s) 135
BspQI GCTCTTC 1 cut(s) 417
BssECI CCNNGG 4 cut(s) 79, 116, 169, 232
BssHII GCGCGC 1 cut(s) 18
BssMI GATC 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 89, 417
Bst6I CTCTTC 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 2 cut(s) 20, 22
BstKTI GATC 1 cut(s) 130
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 3 cut(s) 165, 305, 314
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 3 cut(s) 79, 116, 232
BstUI CGCG 1 cut(s) 20
BsuI GTATCC 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 169
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 345
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 20
CfoI GCGC 2 cut(s) 20, 22
Cfr13I GGNCC 1 cut(s) 356
CpoI CGGWCCG 1 cut(s) 356
Csp6I GTAC 1 cut(s) 367
CspI CGGWCCG 1 cut(s) 356
CviAII CATG 2 cut(s) 49, 170
CviJI RGCY 6 cut(s) 5, 137, 215, 317, 350, 388
CviKI_1 RGCY 6 cut(s) 5, 137, 215, 317, 350, 388
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 1 cut(s) 396
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 2 cut(s) 52, 173
FaiI YATR 7 cut(s) 10, 50, 150, 171, 284, 311, 420
FatI CATG 2 cut(s) 48, 169
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 4 cut(s) 218, 269, 330, 374
GlaI GCGC 2 cut(s) 19, 21
GluI GCNGC 1 cut(s) 138
HapII CCGG 2 cut(s) 81, 118
HhaI GCGC 2 cut(s) 20, 22
Hin1II CATG 2 cut(s) 52, 173
Hin6I GCGC 2 cut(s) 18, 20
HinP1I GCGC 2 cut(s) 18, 20
HinfI GANTC 1 cut(s) 228
HpaII CCGG 2 cut(s) 81, 118
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 3 cut(s) 40, 278, 356
Hpy188III TCNNGA 3 cut(s) 29, 74, 406
Hpy8I GTNNAC 1 cut(s) 369
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 2 cut(s) 89, 417
HpyCH4IV ACGT 1 cut(s) 249
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 305, 314
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 2 cut(s) 52, 173
HspAI GCGC 2 cut(s) 18, 20
Kzo9I GATC 1 cut(s) 127
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 6 cut(s) 94, 131, 169, 207, 219, 246
LweI GCATC 1 cut(s) 286
MaeI CTAG 4 cut(s) 218, 269, 330, 374
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 2 cut(s) 83, 102
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 2 cut(s) 335, 434
MluCI AATT 3 cut(s) 151, 279, 344
MnlI CCTC 4 cut(s) 118, 125, 357, 405
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 53
MspI CCGG 2 cut(s) 81, 118
MspR9I CCNGG 3 cut(s) 81, 118, 234
MvaI CCWGG 1 cut(s) 234
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 3 cut(s) 165, 305, 314
NciI CCSGG 2 cut(s) 81, 118
NcoI CCATGG 1 cut(s) 169
NdeII GATC 1 cut(s) 127
NlaIII CATG 2 cut(s) 52, 173
NlaIV GGNNCC 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 102
NspI RCATGY 1 cut(s) 52
PauI GCGCGC 1 cut(s) 18
PciI ACATGT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 1 cut(s) 228
PkrI GCNGC 1 cut(s) 139
PscI ACATGT 1 cut(s) 48
PsiI TTATAA 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 1 cut(s) 356
PteI GCGCGC 1 cut(s) 18
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 53
Rsr2I CGGWCCG 1 cut(s) 356
RsrII CGGWCCG 1 cut(s) 356
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 3 cut(s) 81, 118, 234
SetI ASST 5 cut(s) 114, 217, 252, 319, 368
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 3 cut(s) 151, 279, 344
SsiI CCGC 2 cut(s) 138, 359
SspMI CTAG 4 cut(s) 218, 269, 330, 374
StyD4I CCNGG 3 cut(s) 79, 116, 232
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 2 cut(s) 89, 417
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 130, 177, 262
TasI AATT 3 cut(s) 151, 279, 344
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 1 cut(s) 102
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 2 cut(s) 210, 435
TspRI CASTG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 356
XceI RCATGY 1 cut(s) 52
XspI CTAG 4 cut(s) 218, 269, 330, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.