Rh5BG099300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006937
Physical Location & Seq
Forward (+)
9039634 .. 9056689
17056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG099300.1

Sequence Viewer

Length: 765 bp
ATGGACCTCGCTGCTTTCCGATTGGCTGTGCGAGGCGCCATCAACCCCGATGCTAGAGTGGTGATGGTATGCATGGATAATTGGGAGATGAAGAACCTTGGACTCACATGTCTGGAGGAGTTTATAATAGCCGGTTACTATTATGCTCAGGCTTTGGTTTTTAGAGTTAGGTCCAGCACTCTTGGTGGGATAGTTTATGGAACAGGGGTTGGATATTCTGTGATTATCGGAAGTGGAAAGACACATGCTGCCGAGTTTGTTGAAGCTCATAGAAACATTCTACAGCCTCATACCCTTGTGGAGTCGGCCATAGTTGTTGCTTCGGTTAAGGAAAAGTCCACCGGCGGCCGTGTGCAGAGAGCTAAGATTCCGGTAGGTGGCAATCAAGCAATCATTTTTTGGGATCCCCCATTGTCAGTGCTGCAACTATTAGAGAGGGATTTGAATCGATATAGGCAGCACCTTGAGAATTCTTTCTTCTTTGTGTGCCATGGTTTCCCCTACAACGAAGAGTTTGAACCAATACTTTTACAAGCTGTAGTAGGACAATTCCCGAACTCGCGCATCAACATCATTTCTTATCGAAACCTAGTTATCAGAATCATAACTTTTAACAATGATTGGTCTGCCTTTGAAGCTAGCAGTTTGCCAAGAGATGGGATCCAATGGGAAATTGAACTGGTTCACTTCATATCGCTAGAGTTCTTTGACCCTACTGAGGAGCATATCAGGATACTACGAGAAGAAGAGCAGAGAGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

254

Amino Acids

28.87

Weight (kDa)

5.68

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 125
AasI GACNNNNNNGTC 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 1 cut(s) 562
AciI CCGC 1 cut(s) 345
AclWI GGATC 4 cut(s) 398, 411, 655, 668
AcoI YGGCCR 2 cut(s) 306, 346
AcsI RAATTY 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 36
AfiI CCNNNNNNNGG 3 cut(s) 377, 656, 718
AflIII ACRYGT 1 cut(s) 107
AgsI TTSAA 5 cut(s) 263, 445, 518, 635, 677
AluBI AGCT 4 cut(s) 266, 362, 536, 638
AluI AGCT 4 cut(s) 266, 362, 536, 638
AlwI GGATC 4 cut(s) 398, 411, 655, 668
AoxI GGCC 2 cut(s) 306, 346
ApeKI GCWGC 4 cut(s) 11, 248, 421, 457
ApoI RAATTY 1 cut(s) 469
Asp700I GAANNNNTTC 2 cut(s) 473, 681
AspLEI GCGC 2 cut(s) 38, 564
AspS9I GGNCC 2 cut(s) 4, 171
AsuHPI GGTGA 1 cut(s) 73
AsuNHI GCTAGC 1 cut(s) 638
AvaII GGWCC 2 cut(s) 4, 171
BamHI GGATCC 2 cut(s) 403, 660
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 235, 408, 469
BccI CCATC 3 cut(s) 47, 58, 650
BceAI ACGGC 1 cut(s) 333
BciVI GTATCC 1 cut(s) 726
BfaI CTAG 4 cut(s) 54, 590, 639, 698
BfmI CTRYAG 2 cut(s) 281, 537
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 1 cut(s) 726
BisI GCNGC 5 cut(s) 12, 249, 346, 422, 458
BlsI GCNGC 5 cut(s) 13, 250, 347, 423, 459
Bme18I GGWCC 2 cut(s) 4, 171
BmgT120I GGNCC 2 cut(s) 4, 171
BmiI GGNNCC 3 cut(s) 37, 405, 662
BmsI GCATC 2 cut(s) 40, 573
BmtI GCTAGC 1 cut(s) 642
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 485
Bsa29I ATCGAT 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 97, 490
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 656, 718
Bse118I RCCGGY 2 cut(s) 131, 341
Bse1I ACTGG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 444
BseCI ATCGAT 1 cut(s) 448
BseDI CCNNGG 2 cut(s) 97, 490
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 377, 656, 718
BseMII CTCAG 2 cut(s) 161, 708
BseNI ACTGG 1 cut(s) 684
BseRI GAGGAG 2 cut(s) 131, 734
BseX3I CGGCCG 1 cut(s) 346
BseXI GCAGC 3 cut(s) 235, 408, 469
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 562
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 2 cut(s) 308, 348
BshNI GGYRCC 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 448
BsiEI CGRYCG 1 cut(s) 349
BsiSI CCGG 3 cut(s) 132, 342, 371
BslI CCNNNNNNNGG 3 cut(s) 377, 656, 718
BsnI GGCC 2 cut(s) 308, 348
Bsp143I GATC 2 cut(s) 403, 660
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 1 cut(s) 345
BspANI GGCC 2 cut(s) 308, 348
BspCNI CTCAG 2 cut(s) 160, 709
BspDI ATCGAT 1 cut(s) 448
BspFNI CGCG 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 37, 405, 662
BspOI GCTAGC 1 cut(s) 642
BspPI GGATC 4 cut(s) 398, 411, 655, 668
BspQI GCTCTTC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 131, 341
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 2 cut(s) 131, 341
BssECI CCNNGG 2 cut(s) 97, 490
BssMI GATC 2 cut(s) 403, 660
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 97, 490
Bst6I CTCTTC 2 cut(s) 504, 741
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 640
BstDEI CTNAG 3 cut(s) 147, 363, 717
BstDSI CCRYGG 1 cut(s) 490
BstFNI CGCG 1 cut(s) 562
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 564
BstKTI GATC 2 cut(s) 406, 663
BstMBI GATC 2 cut(s) 403, 660
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 1 cut(s) 635
BstNSI RCATGY 2 cut(s) 111, 248
BstSFI CTRYAG 2 cut(s) 281, 537
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 3 cut(s) 235, 408, 469
BstX2I RGATCY 2 cut(s) 403, 660
BstYI RGATCY 2 cut(s) 403, 660
BstZI CGGCCG 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 448
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 2 cut(s) 308, 348
BsuTUI ATCGAT 1 cut(s) 448
BtgI CCRYGG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 1 cut(s) 640
CfoI GCGC 2 cut(s) 38, 564
Cfr10I RCCGGY 2 cut(s) 131, 341
Cfr13I GGNCC 2 cut(s) 4, 171
ClaI ATCGAT 1 cut(s) 448
CviAII CATG 4 cut(s) 73, 108, 245, 491
DdeI CTNAG 3 cut(s) 147, 363, 717
DinI GGCGCC 1 cut(s) 37
DpnI GATC 2 cut(s) 405, 662
DpnII GATC 2 cut(s) 403, 660
DrdI GACNNNNNNGTC 1 cut(s) 108
DseDI GACNNNNNNGTC 1 cut(s) 108
EaeI YGGCCR 2 cut(s) 306, 346
EagI CGGCCG 1 cut(s) 346
Eam1104I CTCTTC 2 cut(s) 504, 741
EarI CTCTTC 2 cut(s) 504, 741
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 2 cut(s) 97, 490
Eco47I GGWCC 2 cut(s) 4, 171
Eco52I CGGCCG 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 469
EcoT14I CCWWGG 2 cut(s) 97, 490
EcoT22I ATGCAT 1 cut(s) 74
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 97, 490
FaeI CATG 4 cut(s) 76, 111, 248, 494
FatI CATG 4 cut(s) 72, 107, 244, 490
Fnu4HI GCNGC 5 cut(s) 12, 249, 346, 422, 458
Fsp4HI GCNGC 5 cut(s) 12, 249, 346, 422, 458
FspBI CTAG 4 cut(s) 54, 590, 639, 698
GlaI GCGC 2 cut(s) 37, 563
GluI GCNGC 5 cut(s) 12, 249, 346, 422, 458
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 308, 348
HapII CCGG 3 cut(s) 132, 342, 371
HhaI GCGC 2 cut(s) 38, 564
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 4 cut(s) 76, 111, 248, 494
Hin6I GCGC 2 cut(s) 36, 562
HinP1I GCGC 2 cut(s) 36, 562
HinfI GANTC 5 cut(s) 102, 302, 367, 445, 600
HpaII CCGG 3 cut(s) 132, 342, 371
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 339, 685
Hpy188I TCNGA 3 cut(s) 20, 230, 599
Hpy188III TCNNGA 3 cut(s) 113, 553, 730
Hpy8I GTNNAC 2 cut(s) 339, 685
HpyCH4V TGCA 3 cut(s) 72, 355, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 635
HpyF3I CTNAG 3 cut(s) 147, 363, 717
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 4 cut(s) 76, 111, 248, 494
HspAI GCGC 2 cut(s) 36, 562
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 403, 660
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 1 cut(s) 721
LpnPI CCDG 9 cut(s) 98, 134, 145, 187, 189, 355, 384, 665, 715
Lsp1109I GCAGC 3 cut(s) 235, 408, 469
LweI GCATC 2 cut(s) 40, 573
MaeI CTAG 4 cut(s) 54, 590, 639, 698
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 405, 662
MboI GATC 2 cut(s) 403, 660
MboII GAAGA 5 cut(s) 103, 469, 521, 755, 758
MflI RGATCY 2 cut(s) 403, 660
MluCI AATT 4 cut(s) 79, 469, 548, 672
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 2 cut(s) 96, 311
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 6 cut(s) 17, 26, 109, 297, 429, 712
Mph1103I ATGCAT 1 cut(s) 74
MroXI GAANNNNTTC 2 cut(s) 473, 681
MseI TTAA 2 cut(s) 327, 612
MspI CCGG 3 cut(s) 132, 342, 371
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 1 cut(s) 635
NarI GGCGCC 1 cut(s) 36
NcoI CCATGG 1 cut(s) 490
NdeII GATC 2 cut(s) 403, 660
NheI GCTAGC 1 cut(s) 638
NlaIII CATG 4 cut(s) 76, 111, 248, 494
NlaIV GGNNCC 3 cut(s) 37, 405, 662
NmeAIII GCCGAG 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 2 cut(s) 111, 248
PciI ACATGT 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 741
PdmI GAANNNNTTC 2 cut(s) 473, 681
PfeI GAWTC 3 cut(s) 367, 445, 600
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 5 cut(s) 13, 250, 347, 423, 459
PleI GAGTC 2 cut(s) 96, 310
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 2 cut(s) 96, 310
PscI ACATGT 1 cut(s) 107
PsiI TTATAA 1 cut(s) 125
PspN4I GGNNCC 3 cut(s) 37, 405, 662
PspPI GGNCC 2 cut(s) 4, 171
PsuI RGATCY 2 cut(s) 403, 660
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 2 cut(s) 327, 612
SatI GCNGC 5 cut(s) 12, 249, 346, 422, 458
Sau3AI GATC 2 cut(s) 403, 660
Sau96I GGNCC 2 cut(s) 4, 171
SchI GAGTC 2 cut(s) 96, 311
SfaNI GCATC 2 cut(s) 40, 573
SfcI CTRYAG 2 cut(s) 281, 537
SfoI GGCGCC 1 cut(s) 37
SgrAI CRCCGGYG 1 cut(s) 341
SinI GGWCC 2 cut(s) 4, 171
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 4 cut(s) 79, 469, 548, 672
SsiI CCGC 1 cut(s) 345
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 4 cut(s) 54, 590, 639, 698
StyI CCWWGG 2 cut(s) 97, 490
TaqI TCGA 2 cut(s) 448, 583
TasI AATT 4 cut(s) 79, 469, 548, 672
TauI GCSGC 1 cut(s) 348
TfiI GAWTC 3 cut(s) 367, 445, 600
Tru1I TTAA 2 cut(s) 327, 612
Tru9I TTAA 2 cut(s) 327, 612
TscAI CASTG 1 cut(s) 423
TseI GCWGC 4 cut(s) 11, 248, 421, 457
TspDTI ATGAA 2 cut(s) 104, 679
TspRI CASTG 1 cut(s) 423
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 4, 171
XapI RAATTY 1 cut(s) 469
XceI RCATGY 2 cut(s) 111, 248
XmnI GAANNNNTTC 2 cut(s) 473, 681
XspI CTAG 4 cut(s) 54, 590, 639, 698
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.