Rroxscaffold_2G00116070

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005556
Physical Location & Seq
Reverse (-)
42793124 .. 42796570
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116070.1

Sequence Viewer

Length: 552 bp
ATGACTCAAGAAAGTGCTCTTGCTTCCACCACGGATAGGTCTATTTCCATCACTTTGACCGACCTGGATGTGCTTGATTGTCCCATTTGCTATGAATCCTTGACCATCCCCGTCTTTCAGGAGCATCTGCTCGAAGATGAGGATATTGTTCAGATTATCAAAAAGGCTTTATCAGTTTTGAAATTGCAGAAGGGCAATCAAATGCCCGAAGATTACCCCAGATCTTTGAGAGAAGCAGCCGCAGTTTTCCAGCACGCTCAAGTATACGACTCTAACAAGAAAATTGTCAAGCATATGAGACCCATACCACAAGAGCTTGTGGAGTCTCTTGATGGAGAACTTGATTTCCTTGGATCAGAAATCCCTTCATCAATAGCCATTGCAATTGCTGAAGGAAAATTGGACCCCATAAGTATGGAGGCCTTTGATCATTTCCCTAGTTCCTTTTCTAGACATCGGCTCACTGGCCATTCTATTTTCTATGACCACTTCTATGATTCGGGTGATTCCATTCATGATCAGATTGTGAAACTGGTACAGCAGTACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

183

Amino Acids

20.66

Weight (kDa)

4.8

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 264
AciI CCGC 1 cut(s) 240
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 2 cut(s) 537, 545
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 63
AloI GAACNNNNNNTCC 2 cut(s) 330, 362
AluBI AGCT 1 cut(s) 316
AluI AGCT 1 cut(s) 316
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 292, 330
AlwI GGATC 1 cut(s) 361
AoxI GGCC 2 cut(s) 420, 466
ApeKI GCWGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 403
BaeI ACNNNNGTAYC 1 cut(s) 527
BalI TGGCCA 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 248
BccI CCATC 3 cut(s) 56, 113, 326
BciT130I CCWGG 1 cut(s) 65
BclI TGATCA 2 cut(s) 427, 517
BcoDI GTCTC 2 cut(s) 292, 330
BfaI CTAG 3 cut(s) 438, 450, 550
BglII AGATCT 1 cut(s) 221
BisI GCNGC 2 cut(s) 237, 240
BlsI GCNGC 2 cut(s) 238, 241
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 30, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 2 cut(s) 469, 537
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 30, 349
BseGI GGATG 2 cut(s) 73, 105
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 469, 537
BseXI GCAGC 1 cut(s) 248
BshFI GGCC 2 cut(s) 422, 468
BsiHKAI GWGCWC 1 cut(s) 19
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 2 cut(s) 292, 330
BsmFI GGGAC 1 cut(s) 66
BsnI GGCC 2 cut(s) 422, 468
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 4 cut(s) 221, 353, 427, 517
BspACI CCGC 1 cut(s) 240
BspANI GGCC 2 cut(s) 422, 468
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 2 cut(s) 469, 537
BssECI CCNNGG 2 cut(s) 30, 349
BssMI GATC 4 cut(s) 221, 353, 427, 517
BssNAI GTATAC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 349
Bst1107I GTATAC 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 255
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 73, 105
BstKTI GATC 4 cut(s) 224, 356, 430, 520
BstMAI GTCTC 2 cut(s) 292, 330
BstMBI GATC 4 cut(s) 221, 353, 427, 517
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 1 cut(s) 221
BstZ17I GTATAC 1 cut(s) 265
BsuRI GGCC 2 cut(s) 422, 468
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 2 cut(s) 73, 105
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 255
CciI TCATGA 1 cut(s) 514
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 536, 544
CviAII CATG 1 cut(s) 515
CviJI RGCY 7 cut(s) 167, 239, 316, 377, 422, 460, 468
CviKI_1 RGCY 7 cut(s) 167, 239, 316, 377, 422, 460, 468
CviQI GTAC 2 cut(s) 536, 544
DpnI GATC 4 cut(s) 223, 355, 429, 519
DpnII GATC 4 cut(s) 221, 353, 427, 517
EaeI YGGCCR 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 1 cut(s) 518
FaqI GGGAC 1 cut(s) 66
FatI CATG 1 cut(s) 514
FauNDI CATATG 1 cut(s) 294
FbaI TGATCA 2 cut(s) 427, 517
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 237, 240
FokI GGATG 2 cut(s) 80, 92
Fsp4HI GCNGC 2 cut(s) 237, 240
FspBI CTAG 3 cut(s) 438, 450, 550
GluI GCNGC 2 cut(s) 237, 240
HaeIII GGCC 2 cut(s) 422, 468
Hin1II CATG 1 cut(s) 518
HinfI GANTC 6 cut(s) 4, 95, 269, 323, 497, 506
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 2 cut(s) 265, 546
Hpy188I TCNGA 3 cut(s) 153, 358, 522
Hpy188III TCNNGA 5 cut(s) 8, 119, 329, 450, 515
Hpy8I GTNNAC 2 cut(s) 265, 546
HpyAV CCTTC 3 cut(s) 184, 375, 386
HpyCH4V TGCA 2 cut(s) 187, 383
Hsp92II CATG 1 cut(s) 518
Ksp22I TGATCA 2 cut(s) 427, 517
Kzo9I GATC 4 cut(s) 221, 353, 427, 517
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 7 cut(s) 50, 77, 104, 232, 263, 450, 518
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 1 cut(s) 133
MaeI CTAG 3 cut(s) 438, 450, 550
MalI GATC 4 cut(s) 223, 355, 429, 519
MboI GATC 4 cut(s) 221, 353, 427, 517
MboII GAAGA 2 cut(s) 146, 221
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 468
MluCI AATT 4 cut(s) 182, 282, 384, 398
MluNI TGGCCA 1 cut(s) 468
MlyI GAGTC 2 cut(s) 263, 332
MnlI CCTC 2 cut(s) 133, 412
Mox20I TGGCCA 1 cut(s) 468
MscI TGGCCA 1 cut(s) 468
MslI CAYNNNNRTG 2 cut(s) 413, 492
Msp20I TGGCCA 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 65
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 294
NdeII GATC 4 cut(s) 221, 353, 427, 517
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 1 cut(s) 405
PagI TCATGA 1 cut(s) 514
PceI AGGCCT 1 cut(s) 422
PfeI GAWTC 3 cut(s) 95, 497, 506
PkrI GCNGC 2 cut(s) 238, 241
PleI GAGTC 2 cut(s) 263, 331
PpsI GAGTC 2 cut(s) 263, 331
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 403
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 537, 545
RsaNI GTAC 2 cut(s) 536, 544
RseI CAYNNNNRTG 2 cut(s) 413, 492
SatI GCNGC 2 cut(s) 237, 240
Sau3AI GATC 4 cut(s) 221, 353, 427, 517
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 2 cut(s) 263, 332
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 1 cut(s) 19
SetI ASST 4 cut(s) 41, 66, 318, 551
SfaNI GCATC 1 cut(s) 133
SinI GGWCC 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 413, 492
SmlI CTYRAG 2 cut(s) 6, 258
SmoI CTYRAG 2 cut(s) 6, 258
Sse9I AATT 4 cut(s) 182, 282, 384, 398
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 1 cut(s) 240
SspMI CTAG 3 cut(s) 438, 450, 550
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 349
TaqI TCGA 1 cut(s) 132
TaqII GACCGA 1 cut(s) 74
TasI AATT 4 cut(s) 182, 282, 384, 398
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 3 cut(s) 95, 497, 506
TscAI CASTG 1 cut(s) 469
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 3 cut(s) 108, 357, 503
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 403
XbaI TCTAGA 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 3 cut(s) 438, 450, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.