Rroxscaffold_2G00120080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006385
Physical Location & Seq
Forward (+)
52159184 .. 52166808
7625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120080.1

Sequence Viewer

Length: 1332 bp
ATGGGACTGAGGACAGCCGCAGCAAGATTAGGGTTACAATTAGCGAGTGCAAGGAAGGATACGAAGCAGAAATTGATGATGATCAAGAAAATTAGCAGCAGCAGCCTTTCGCAGTTTATGAGAAGATCCAGTAGTTTAGCAAGGGCAGCAGATACATGGGAGAAGAAGGGTATGAAATCGGAATCGGAAATGGAATTGGAATTGAATAAGGAGAAGACTGTATACTTATGTTTCTTTGAGTGGAGTCAATGCGGTGCTGTTGAATACCTGAATCAAGTTATTCGCCCCATCAAATTGAGCCAGGTTAATAGTAATGATGATGATGATATTGCCGTGGAGTTGCGCGAAGCAGCTTATAAGAGGTCCAAGGTTAATTTATGGTATTCCAATTGTAGTCTCTTCGGCTCCCAAATTCTTGTCGCCGGCGGCCGTAGAAGGCCCCCGCCAGGCATTATGGGTCCTGCTATTGGCCGTAGAGATATTTATTGGTTTGACACGGCCCAGGGGAAATTCCAGGTTTCCTCCCCAACGAAAACCAAAATCAAGAGCTTCTCAACTGGTAAACAAGCCGTGCTGTTGGGGGAGCTCAACCGCGAACTCTATTGCATCGAGAACGACCCCTTTAGCACGACTCCTCCCTCTCAGCCTTTTCAGGTATTGGACCCCGAAAACGACCTCAAGTGGGAGGACTTCACTGCGTCTTTTTTGGAGGGGAGCAACAAAATCTTAGGTTGGGTGTGGCAGCAGCCTGGAGTGTTTTGCTTTGATGACACCAAGCCTGAAAATGGATGGATAGAGTCACCTTCTTGTCTGGGAAACAGCCTTCCCTTTCTTGACCACGGGCATTTCTTCGTCCGCCACCATCATCCCAATCTTGGTGCTTTCAACATAATGTTTCACTATCATCCTGACAATGCACCTCTCCTTGTATCCACCTTTTTCATGCCAAACAACTGTGATTCTCTCCATCCTATCAACAAACCTCTTCAGCTGCCAGAATTGTGTTATGAATTTCGTGAGGATGAGTATGATGACACCTTCTCTACCGATTTTCAATTTGTCCATCTCGGAGCTCAAAAAGTATGCCTTATTTTGCACAAATTCTTCTGCCCATCAACAGGGGAGGTTCAATGTGGAAACATGGGGAGTCTTCTTCTCATAACATTTGAATATGACGTTATTAGTAATACTACTGTCGACTCCAAATCCACCTTGGAGGTCCAGACCCGCTTGCTCGGGACCCACCGCCGTCGATACTTTACCAGGCAGCTGCGTAAACCAGATTATACATGGACCAACACATCATACCTGACTGGTGCATTTCTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

443

Amino Acids

50.6

Weight (kDa)

7.14

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AccI GTMKAC 2 cut(s) 222, 1197
AccII CGCG 2 cut(s) 345, 594
AciI CCGC 8 cut(s) 18, 252, 426, 443, 592, 856, 1228, 1246
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 2 cut(s) 427, 469
AcsI RAATTY 4 cut(s) 411, 509, 1010, 1100
AcuI CTGAAG 1 cut(s) 971
AfiI CCNNNNNNNGG 6 cut(s) 446, 467, 682, 785, 875, 1118
AgsI TTSAA 6 cut(s) 205, 263, 886, 1055, 1130, 1169
AjnI CCWGG 6 cut(s) 300, 445, 501, 513, 748, 1262
AluBI AGCT 6 cut(s) 353, 549, 586, 991, 1073, 1270
AluI AGCT 6 cut(s) 353, 549, 586, 991, 1073, 1270
Alw21I GWGCWC 2 cut(s) 588, 1075
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 1235
AoxI GGCC 4 cut(s) 427, 437, 469, 498
ApoI RAATTY 4 cut(s) 411, 509, 1010, 1100
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 8 cut(s) 363, 438, 458, 499, 661, 1219, 1239, 1293
AsuHPI GGTGA 1 cut(s) 792
AvaI CYCGRG 1 cut(s) 1235
AvaII GGWCC 6 cut(s) 363, 458, 661, 1219, 1239, 1293
BanII GRGCYC 2 cut(s) 588, 1075
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
BbsI GAAGAC 2 cut(s) 221, 1142
Bbv12I GWGCWC 2 cut(s) 588, 1075
BccI CCATC 6 cut(s) 296, 783, 870, 975, 1071, 1120
BceAI ACGGC 6 cut(s) 317, 414, 456, 513, 554, 1233
BciT130I CCWGG 6 cut(s) 302, 447, 503, 515, 750, 1264
BciVI GTATCC 2 cut(s) 52, 940
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 401
BfuI GTATCC 2 cut(s) 52, 940
Bme1390I CCNGG 6 cut(s) 302, 447, 503, 515, 750, 1264
Bme18I GGWCC 6 cut(s) 363, 458, 661, 1219, 1239, 1293
BmeT110I CYCGRG 1 cut(s) 1235
BmgT120I GGNCC 8 cut(s) 363, 438, 458, 499, 661, 1219, 1239, 1293
BmiI GGNNCC 6 cut(s) 406, 440, 459, 663, 1240, 1241
BmrFI CCNGG 6 cut(s) 302, 447, 503, 515, 750, 1264
BmsI GCATC 1 cut(s) 615
BpiI GAAGAC 2 cut(s) 221, 1142
BpmI CTGGAG 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 662
BsaBI GATNNNNATC 1 cut(s) 80
BsaJI CCNNGG 6 cut(s) 333, 366, 501, 502, 838, 1212
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 6 cut(s) 446, 467, 682, 785, 875, 1118
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 3 cut(s) 129, 562, 1318
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 6 cut(s) 302, 447, 503, 515, 750, 1264
BseDI CCNNGG 6 cut(s) 333, 366, 501, 502, 838, 1212
BseGI GGATG 5 cut(s) 794, 865, 904, 967, 1027
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 6 cut(s) 446, 467, 682, 785, 875, 1118
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 3 cut(s) 129, 562, 1318
BseRI GAGGAG 1 cut(s) 624
BseX3I CGGCCG 1 cut(s) 427
Bsh1236I CGCG 2 cut(s) 345, 594
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 4 cut(s) 429, 439, 471, 500
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 588, 1075
BsiHKCI CYCGRG 1 cut(s) 1235
BsiSI CCGG 1 cut(s) 423
BslFI GGGAC 2 cut(s) 18, 1252
BslI CCNNNNNNNGG 6 cut(s) 446, 467, 682, 785, 875, 1118
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 18, 1252
BsnI GGCC 4 cut(s) 429, 439, 471, 500
BsoBI CYCGRG 1 cut(s) 1235
Bsp1286I GDGCHC 2 cut(s) 588, 1075
Bsp143I GATC 3 cut(s) 81, 125, 1326
BspACI CCGC 8 cut(s) 18, 252, 426, 443, 592, 856, 1228, 1246
BspANI GGCC 4 cut(s) 429, 439, 471, 500
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 2 cut(s) 345, 594
BspLI GGNNCC 6 cut(s) 406, 440, 459, 663, 1240, 1241
BspPI GGATC 1 cut(s) 120
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 3 cut(s) 129, 562, 1318
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 6 cut(s) 333, 366, 501, 502, 838, 1212
BssMI GATC 3 cut(s) 81, 125, 1326
BssNAI GTATAC 1 cut(s) 223
BssT1I CCWWGG 2 cut(s) 366, 1212
Bst1107I GTATAC 1 cut(s) 223
Bst2UI CCWGG 6 cut(s) 302, 447, 503, 515, 750, 1264
Bst4CI ACNGT 3 cut(s) 220, 956, 1195
Bst6I CTCTTC 2 cut(s) 404, 990
BstC8I GCNNGC 2 cut(s) 424, 1232
BstDEI CTNAG 3 cut(s) 8, 642, 727
BstDSI CCRYGG 2 cut(s) 333, 838
BstF5I GGATG 5 cut(s) 794, 865, 904, 967, 1027
BstFNI CGCG 2 cut(s) 345, 594
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 3 cut(s) 84, 128, 1329
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 3 cut(s) 81, 125, 1326
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 2 cut(s) 102, 146
BstNI CCWGG 6 cut(s) 302, 447, 503, 515, 750, 1264
BstSCI CCNGG 6 cut(s) 300, 445, 501, 513, 748, 1262
BstUI CGCG 2 cut(s) 345, 594
BstV2I GAAGAC 2 cut(s) 221, 1142
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BstZ17I GTATAC 1 cut(s) 223
BstZI CGGCCG 1 cut(s) 427
BsuI GTATCC 2 cut(s) 52, 940
BsuRI GGCC 4 cut(s) 429, 439, 471, 500
BtgI CCRYGG 2 cut(s) 333, 838
BtsCI GGATG 5 cut(s) 794, 865, 904, 967, 1027
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 2 cut(s) 424, 1232
CfoI GCGC 1 cut(s) 345
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 8 cut(s) 363, 438, 458, 499, 661, 1219, 1239, 1293
CseI GACGC 1 cut(s) 687
CviAII CATG 4 cut(s) 156, 943, 1141, 1290
DdeI CTNAG 3 cut(s) 8, 642, 727
DpnI GATC 3 cut(s) 83, 127, 1328
DpnII GATC 3 cut(s) 81, 125, 1326
EaeI YGGCCR 2 cut(s) 427, 469
EagI CGGCCG 1 cut(s) 427
Eam1104I CTCTTC 2 cut(s) 404, 990
EarI CTCTTC 2 cut(s) 404, 990
EciI GGCGGA 1 cut(s) 845
Ecl136II GAGCTC 2 cut(s) 586, 1073
EclXI CGGCCG 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 366, 1212
Eco24I GRGCYC 2 cut(s) 588, 1075
Eco47I GGWCC 6 cut(s) 363, 458, 661, 1219, 1239, 1293
Eco52I CGGCCG 1 cut(s) 427
Eco53kI GAGCTC 2 cut(s) 586, 1073
Eco57I CTGAAG 1 cut(s) 971
Eco88I CYCGRG 1 cut(s) 1235
EcoICRI GAGCTC 2 cut(s) 586, 1073
EcoO109I RGGNCCY 3 cut(s) 438, 458, 1239
EcoRII CCWGG 6 cut(s) 300, 445, 501, 513, 748, 1262
EcoT14I CCWWGG 2 cut(s) 366, 1212
EcoT38I GRGCYC 2 cut(s) 588, 1075
ErhI CCWWGG 2 cut(s) 366, 1212
FaeI CATG 4 cut(s) 159, 946, 1144, 1293
FalI AAGNNNNNCTT 2 cut(s) 1071, 1103
FaqI GGGAC 2 cut(s) 18, 1252
FatI CATG 4 cut(s) 155, 942, 1140, 1289
FauI CCCGC 2 cut(s) 450, 1235
FbaI TGATCA 1 cut(s) 81
FblI GTMKAC 2 cut(s) 222, 1197
FokI GGATG 5 cut(s) 801, 852, 891, 954, 1034
FriOI GRGCYC 2 cut(s) 588, 1075
GlaI GCGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 4 cut(s) 429, 439, 471, 500
HapII CCGG 1 cut(s) 423
HgaI GACGC 1 cut(s) 687
HhaI GCGC 1 cut(s) 345
Hin1II CATG 4 cut(s) 159, 946, 1144, 1293
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HincII GTYRAC 1 cut(s) 1198
HindII GTYRAC 1 cut(s) 1198
HinfI GANTC 8 cut(s) 182, 244, 271, 631, 797, 959, 1147, 1199
HpaII CCGG 1 cut(s) 423
HphI GGTGA 1 cut(s) 792
Hpy166II GTNNAC 4 cut(s) 223, 563, 1198, 1277
Hpy188I TCNGA 4 cut(s) 181, 187, 1070, 1326
Hpy188III TCNNGA 8 cut(s) 85, 544, 610, 833, 908, 1016, 1222, 1237
Hpy8I GTNNAC 4 cut(s) 223, 563, 1198, 1277
Hpy99I CGWCG 1 cut(s) 1254
HpyAV CCTTC 6 cut(s) 49, 160, 429, 813, 833, 1048
HpyCH4III ACNGT 3 cut(s) 220, 956, 1195
HpyCH4IV ACGT 1 cut(s) 1176
HpyCH4V TGCA 5 cut(s) 50, 606, 917, 1096, 1319
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 146
HpyF3I CTNAG 3 cut(s) 8, 642, 727
HpySE526I ACGT 1 cut(s) 1176
Hsp92II CATG 4 cut(s) 159, 946, 1144, 1293
HspAI GCGC 1 cut(s) 343
KflI GGGWCCC 1 cut(s) 1239
KroI GCCGGC 1 cut(s) 422
KroNI GCCGGC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 3 cut(s) 81, 125, 1326
LmnI GCTCC 4 cut(s) 410, 583, 714, 1070
LweI GCATC 1 cut(s) 615
MaeII ACGT 1 cut(s) 1176
MaeIII GTNAC 2 cut(s) 33, 798
MalI GATC 3 cut(s) 83, 127, 1328
MboI GATC 3 cut(s) 81, 125, 1326
MboII GAAGA 9 cut(s) 135, 175, 226, 391, 841, 977, 1096, 1142, 1145
MfeI CAATTG 1 cut(s) 388
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 2 cut(s) 588, 1075
MlyI GAGTC 5 cut(s) 253, 625, 806, 1156, 1193
MreI CGCCGGCG 1 cut(s) 422
MroNI GCCGGC 1 cut(s) 422
MseI TTAA 2 cut(s) 306, 372
MspA1I CMGCKG 2 cut(s) 991, 1270
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 6 cut(s) 302, 447, 503, 515, 750, 1264
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 6 cut(s) 302, 447, 503, 515, 750, 1264
MvnI CGCG 2 cut(s) 345, 594
MwoI GCNNNNNNNGC 2 cut(s) 102, 146
NaeI GCCGGC 1 cut(s) 424
NdeII GATC 3 cut(s) 81, 125, 1326
NgoMIV GCCGGC 1 cut(s) 422
NlaIII CATG 4 cut(s) 159, 946, 1144, 1293
NlaIV GGNNCC 6 cut(s) 406, 440, 459, 663, 1240, 1241
NmuCI GTSAC 1 cut(s) 798
PasI CCCWGGG 1 cut(s) 502
PdiI GCCGGC 1 cut(s) 424
PfeI GAWTC 3 cut(s) 182, 271, 959
PleI GAGTC 5 cut(s) 252, 625, 805, 1155, 1193
PpsI GAGTC 5 cut(s) 252, 625, 805, 1155, 1193
PpuMI RGGWCCY 2 cut(s) 458, 1239
PsiI TTATAA 1 cut(s) 357
Psp124BI GAGCTC 2 cut(s) 588, 1075
Psp5II RGGWCCY 2 cut(s) 458, 1239
Psp6I CCWGG 6 cut(s) 300, 445, 501, 513, 748, 1262
PspGI CCWGG 6 cut(s) 300, 445, 501, 513, 748, 1262
PspN4I GGNNCC 6 cut(s) 406, 440, 459, 663, 1240, 1241
PspPI GGNCC 8 cut(s) 363, 438, 458, 499, 661, 1219, 1239, 1293
PspPPI RGGWCCY 2 cut(s) 458, 1239
PsuI RGATCY 1 cut(s) 125
PvuII CAGCTG 2 cut(s) 991, 1270
SacI GAGCTC 2 cut(s) 588, 1075
SalI GTCGAC 1 cut(s) 1196
SaqAI TTAA 2 cut(s) 306, 372
Sau3AI GATC 3 cut(s) 81, 125, 1326
Sau96I GGNCC 8 cut(s) 363, 438, 458, 499, 661, 1219, 1239, 1293
SchI GAGTC 5 cut(s) 253, 625, 806, 1156, 1193
ScrFI CCNGG 6 cut(s) 302, 447, 503, 515, 750, 1264
SduI GDGCHC 2 cut(s) 588, 1075
SfaNI GCATC 1 cut(s) 615
SgrAI CRCCGGYG 1 cut(s) 422
SinI GGWCC 6 cut(s) 363, 458, 661, 1219, 1239, 1293
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
SsiI CCGC 8 cut(s) 18, 252, 426, 443, 592, 856, 1228, 1246
SstI GAGCTC 2 cut(s) 588, 1075
StyD4I CCNGG 6 cut(s) 300, 445, 501, 513, 748, 1262
StyI CCWWGG 2 cut(s) 366, 1212
TaaI ACNGT 3 cut(s) 220, 956, 1195
TaiI ACGT 1 cut(s) 1179
TaqI TCGA 3 cut(s) 609, 1197, 1252
TauI GCSGC 2 cut(s) 20, 429
TfiI GAWTC 3 cut(s) 182, 271, 959
Tru1I TTAA 2 cut(s) 306, 372
Tru9I TTAA 2 cut(s) 306, 372
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 1 cut(s) 798
Tsp45I GTSAC 1 cut(s) 798
TspDTI ATGAA 3 cut(s) 188, 931, 1023
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 6 cut(s) 363, 458, 661, 1219, 1239, 1293
XapI RAATTY 4 cut(s) 411, 509, 1010, 1100
XcmI CCANNNNNNNNNTGG 2 cut(s) 1210, 1287
XmiI GTMKAC 2 cut(s) 222, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.