Rh2BG253300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006385
Physical Location & Seq
Reverse (-)
26137507 .. 26155997
18491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG253300.1

Sequence Viewer

Length: 1251 bp
ATGTCGGTTGAAGCTTATGCAATGGCAGGGATGGATTATGCGAAGTCCGGGATTGATCTAGAAGAGTTGGAGAATGGTGCTTGGAAAGAGCCTCCATATCTTCTAAGCGAGCAAAGCTTAAGCTATGGAGTTCAGATTCAGAACAATACAGGTAGTAAAGCAGACACTACGATGGCGGACAAAGAAGAAGAGATGAAAGCAAGGATGCGGGCATGGGCAAAGGCTGTTGCTTCCATGAATGAGATTATCATGGCTTTTAGCTCCAAAATAGAGGAATCCCTTGAGGGCTCGCATGGTATTGGACCCCGAAAACGACCTCAAGTGGCGGTCACTGGAAGCGCCTCCCATTATCCGCGGCAGAGAGTCAGAGGACTTCACTGCGTGTTTTTTGGAGGGGAGCAACAAAATCTTAGGTTGGGTGTGGTATCAGCCTGGAGCCCTGGAGTGTTTTGCTTTGATGACACCAAGCCTGAAAATGGATGGATAGAGTCACCTTCTTGTCTGGGAAACAGCCTTCCCTTTCTTGACTATGGGCCTTTCTTCGTCCTCCACCATCAGCCCAATCTTGGTGCTTTCAACCTAATGTTTAACTATGATCCGGACGATGCACCTCTCCTTGTATCCGCCTTTTTCATGCCAAACAACTGTGATTCTTTCCATCCTATCAACAAACCTCTTCAGCTGCCAAAATTATGTTATGAATTTCGAGGGGATGAGTATGATGGCACCTTCTCTACCGATTTTCAATTTGTCCATCTCAGAGCTCAAAAAGTCTGCCTTGTTTTGCACAAATTCTTCTCCCCATCAACAAGGAAGGCTCAATGTGGAAACATGGCGAGTCTTCTTCTCATTATAACATTTGAATATGACATTATTAGTAGTAATACTACTGTCGATTCCAAATCCACCTTGGAGATCCAGACCCGATTGCTCGGGACCCACCGTCGTCGATACTTTACCAGGCAGCTGCGTAAACCAGATGATACATGGACCAACACAGCATACTTGACTGTTTGTAATGAAACAATAGCTATTGGACTACTTCATCTATGGGCTTTTCATGTTAATCTTTATGTTTTCTCATTTGCTGTCACAAAAATAGTCTCATTTCTATATCACAGCTATAGACTGAGAGTGACTTGTTATGCTTACAAAAGACTCTTCAGTCACTCTAAGACTCTTAGACAGAGCACGAGATCTGAGACTGTAATGTTCTTTCTAAGCCAATCGACATGTGCTGCAGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

416

Amino Acids

47.16

Weight (kDa)

7.6

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 854
AasI GACNNNNNNGTC 1 cut(s) 1164
AccB1I GGYRCC 1 cut(s) 725
AccII CGCG 1 cut(s) 355
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 6 cut(s) 176, 208, 326, 353, 355, 624
AclWI GGATC 2 cut(s) 590, 910
AcsI RAATTY 2 cut(s) 701, 791
AcuI CTGAAG 2 cut(s) 662, 1147
AdeI CACNNNGTG 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 476, 566
AflII CTTAAG 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 1232
AgsI TTSAA 4 cut(s) 11, 577, 746, 863
AhdI GACNNNNNGTC 1 cut(s) 942
AjnI CCWGG 3 cut(s) 431, 439, 959
AluBI AGCT 9 cut(s) 14, 117, 123, 261, 682, 764, 967, 1031, 1122
AluI AGCT 9 cut(s) 14, 117, 123, 261, 682, 764, 967, 1031, 1122
Alw21I GWGCWC 2 cut(s) 766, 1193
Alw26I GTCTC 2 cut(s) 1108, 1196
AlwI GGATC 2 cut(s) 590, 910
Ama87I CYCGRG 1 cut(s) 932
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 4 cut(s) 682, 964, 967, 1238
ApoI RAATTY 2 cut(s) 701, 791
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 4 cut(s) 302, 533, 936, 990
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 483
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 3 cut(s) 302, 936, 990
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 3 cut(s) 290, 440, 766
BauI CACGAG 1 cut(s) 1192
BbsI GAAGAC 1 cut(s) 833
Bbv12I GWGCWC 2 cut(s) 766, 1193
BbvI GCAGC 4 cut(s) 669, 954, 976, 1225
BccI CCATC 8 cut(s) 25, 166, 474, 561, 666, 716, 762, 811
BciT130I CCWGG 3 cut(s) 433, 441, 961
BciVI GTATCC 1 cut(s) 631
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 1108, 1196
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 2 cut(s) 1123, 1239
BfoI RGCGCY 1 cut(s) 342
BfrI CTTAAG 1 cut(s) 118
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 1 cut(s) 1196
BisI GCNGC 5 cut(s) 356, 683, 965, 968, 1239
BlsI GCNGC 5 cut(s) 357, 684, 966, 969, 1240
Bme1390I CCNGG 4 cut(s) 49, 433, 441, 961
Bme18I GGWCC 3 cut(s) 302, 936, 990
BmeRI GACNNNNNGTC 1 cut(s) 942
BmeT110I CYCGRG 1 cut(s) 932
BmgT120I GGNCC 4 cut(s) 302, 533, 936, 990
BmiI GGNNCC 5 cut(s) 304, 437, 727, 937, 938
BmrFI CCNGG 4 cut(s) 49, 433, 441, 961
BmsI GCATC 2 cut(s) 195, 595
BpiI GAAGAC 1 cut(s) 833
BpmI CTGGAG 2 cut(s) 454, 462
BpuEI CTTGAG 2 cut(s) 302, 303
BpuMI CCSGG 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 353, 439, 909
BsaWI WCCGGW 1 cut(s) 598
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 566
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 3 cut(s) 433, 441, 961
BseDI CCNNGG 3 cut(s) 353, 439, 909
BseGI GGATG 5 cut(s) 36, 210, 485, 658, 718
BseLI CCNNNNNNNGG 2 cut(s) 476, 566
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 772, 1121, 1191
BseNI ACTGG 1 cut(s) 337
BseXI GCAGC 4 cut(s) 669, 954, 976, 1225
Bsh1236I CGCG 1 cut(s) 355
BshFI GGCC 1 cut(s) 535
BshNI GGYRCC 1 cut(s) 725
BsiHKAI GWGCWC 2 cut(s) 766, 1193
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 2 cut(s) 48, 599
BslFI GGGAC 1 cut(s) 949
BslI CCNNNNNNNGG 2 cut(s) 476, 566
BsmAI GTCTC 2 cut(s) 1108, 1196
BsmFI GGGAC 1 cut(s) 949
BsnI GGCC 1 cut(s) 535
BsoBI CYCGRG 1 cut(s) 932
Bsp1286I GDGCHC 4 cut(s) 290, 440, 766, 1193
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 4 cut(s) 55, 595, 915, 1196
BspACI CCGC 6 cut(s) 176, 208, 326, 353, 355, 624
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 3 cut(s) 771, 1122, 1192
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 1 cut(s) 355
BspLI GGNNCC 5 cut(s) 304, 437, 727, 937, 938
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 2 cut(s) 590, 910
BspT107I GGYRCC 1 cut(s) 725
BspTI CTTAAG 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 353, 439, 909
BssMI GATC 4 cut(s) 55, 595, 915, 1196
BssSI CACGAG 1 cut(s) 1192
BssT1I CCWWGG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 1192
Bst2UI CCWGG 3 cut(s) 433, 441, 961
Bst4CI ACNGT 5 cut(s) 647, 892, 944, 1012, 1207
Bst6I CTCTTC 4 cut(s) 57, 183, 681, 1166
BstAFI CTTAAG 1 cut(s) 118
BstC8I GCNNGC 3 cut(s) 110, 210, 290
BstDEI CTNAG 8 cut(s) 104, 410, 758, 1130, 1173, 1181, 1200, 1220
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 5 cut(s) 36, 210, 485, 658, 718
BstFNI CGCG 1 cut(s) 355
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 4 cut(s) 58, 598, 918, 1199
BstMAI GTCTC 2 cut(s) 1108, 1196
BstMBI GATC 4 cut(s) 55, 595, 915, 1196
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 3 cut(s) 433, 441, 961
BstNSI RCATGY 1 cut(s) 1236
BstSCI CCNGG 4 cut(s) 47, 431, 439, 959
BstSFI CTRYAG 2 cut(s) 1123, 1239
BstUI CGCG 1 cut(s) 355
BstV1I GCAGC 4 cut(s) 669, 954, 976, 1225
BstV2I GAAGAC 1 cut(s) 833
BstX2I RGATCY 2 cut(s) 915, 1196
BstYI RGATCY 2 cut(s) 915, 1196
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 1 cut(s) 535
BtgI CCRYGG 1 cut(s) 353
BtsCI GGATG 5 cut(s) 36, 210, 485, 658, 718
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 330, 376
Cac8I GCNNGC 3 cut(s) 110, 210, 290
CfoI GCGC 1 cut(s) 341
Cfr13I GGNCC 4 cut(s) 302, 533, 936, 990
Cfr42I CCGCGG 1 cut(s) 356
CviAII CATG 9 cut(s) 213, 235, 250, 293, 634, 832, 987, 1061, 1233
DdeI CTNAG 8 cut(s) 104, 410, 758, 1130, 1173, 1181, 1200, 1220
DpnI GATC 4 cut(s) 57, 597, 917, 1198
DpnII GATC 4 cut(s) 55, 595, 915, 1196
DraIII CACNNNGTG 1 cut(s) 382
DrdI GACNNNNNNGTC 1 cut(s) 1164
DriI GACNNNNNGTC 1 cut(s) 942
DseDI GACNNNNNNGTC 1 cut(s) 1164
Eam1104I CTCTTC 4 cut(s) 57, 183, 681, 1166
Eam1105I GACNNNNNGTC 1 cut(s) 942
EarI CTCTTC 4 cut(s) 57, 183, 681, 1166
EciI GGCGGA 2 cut(s) 191, 613
Ecl136II GAGCTC 1 cut(s) 764
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 3 cut(s) 290, 440, 766
Eco47I GGWCC 3 cut(s) 302, 936, 990
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 2 cut(s) 662, 1147
Eco88I CYCGRG 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 764
EcoO109I RGGNCCY 1 cut(s) 936
EcoRII CCWGG 3 cut(s) 431, 439, 959
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 3 cut(s) 290, 440, 766
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 9 cut(s) 216, 238, 253, 296, 637, 835, 990, 1064, 1236
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 1 cut(s) 949
FatI CATG 9 cut(s) 212, 234, 249, 292, 633, 831, 986, 1060, 1232
FauI CCCGC 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 356, 683, 965, 968, 1239
FokI GGATG 5 cut(s) 43, 217, 492, 645, 725
FriOI GRGCYC 3 cut(s) 290, 440, 766
Fsp4HI GCNGC 5 cut(s) 356, 683, 965, 968, 1239
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 340
GluI GCNGC 5 cut(s) 356, 683, 965, 968, 1239
GsuI CTGGAG 2 cut(s) 454, 462
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 1 cut(s) 535
HapII CCGG 2 cut(s) 48, 599
HhaI GCGC 1 cut(s) 341
Hin1II CATG 9 cut(s) 216, 238, 253, 296, 637, 835, 990, 1064, 1236
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HindIII AAGCTT 2 cut(s) 12, 115
HinfI GANTC 9 cut(s) 136, 275, 363, 488, 650, 838, 896, 1158, 1177
HpaII CCGG 2 cut(s) 48, 599
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 1 cut(s) 974
Hpy188I TCNGA 5 cut(s) 135, 141, 368, 761, 1201
Hpy188III TCNNGA 5 cut(s) 59, 524, 599, 919, 934
Hpy8I GTNNAC 1 cut(s) 974
Hpy99I CGWCG 2 cut(s) 948, 951
HpyAV CCTTC 4 cut(s) 504, 524, 739, 808
HpyCH4III ACNGT 5 cut(s) 647, 892, 944, 1012, 1207
HpyCH4V TGCA 4 cut(s) 20, 608, 787, 1241
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 8 cut(s) 104, 410, 758, 1130, 1173, 1181, 1200, 1220
Hsp92II CATG 9 cut(s) 216, 238, 253, 296, 637, 835, 990, 1064, 1236
HspAI GCGC 1 cut(s) 339
KflI GGGWCCC 1 cut(s) 936
Kpn2I TCCGGA 1 cut(s) 598
KspI CCGCGG 1 cut(s) 356
Kzo9I GATC 4 cut(s) 55, 595, 915, 1196
LmnI GCTCC 3 cut(s) 266, 397, 435
Lsp1109I GCAGC 4 cut(s) 669, 954, 976, 1225
LweI GCATC 2 cut(s) 195, 595
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 5 cut(s) 328, 489, 1090, 1135, 1166
MalI GATC 4 cut(s) 57, 597, 917, 1198
MboI GATC 4 cut(s) 55, 595, 915, 1196
MflI RGATCY 2 cut(s) 915, 1196
MhlI GDGCHC 4 cut(s) 290, 440, 766, 1193
MluCI AATT 4 cut(s) 689, 701, 746, 791
MlyI GAGTC 5 cut(s) 372, 497, 847, 1152, 1171
MmeI TCCRAC 1 cut(s) 48
MroI TCCGGA 1 cut(s) 598
MseI TTAA 3 cut(s) 119, 588, 1065
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 3 cut(s) 355, 682, 967
MspCI CTTAAG 1 cut(s) 118
MspI CCGG 2 cut(s) 48, 599
MspR9I CCNGG 4 cut(s) 49, 433, 441, 961
MvaI CCWGG 3 cut(s) 433, 441, 961
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 49
NdeII GATC 4 cut(s) 55, 595, 915, 1196
NlaIII CATG 9 cut(s) 216, 238, 253, 296, 637, 835, 990, 1064, 1236
NlaIV GGNNCC 5 cut(s) 304, 437, 727, 937, 938
NmuCI GTSAC 5 cut(s) 328, 489, 1090, 1135, 1166
NspI RCATGY 1 cut(s) 1236
PciI ACATGT 1 cut(s) 1232
PfeI GAWTC 4 cut(s) 136, 275, 650, 896
PfoI TCCNGGA 1 cut(s) 47
PkrI GCNGC 5 cut(s) 357, 684, 966, 969, 1240
PleI GAGTC 5 cut(s) 371, 496, 846, 1152, 1171
PpsI GAGTC 5 cut(s) 371, 496, 846, 1152, 1171
PpuMI RGGWCCY 1 cut(s) 936
PscI ACATGT 1 cut(s) 1232
PsiI TTATAA 1 cut(s) 854
Psp124BI GAGCTC 1 cut(s) 766
Psp5II RGGWCCY 1 cut(s) 936
Psp6I CCWGG 3 cut(s) 431, 439, 959
PspGI CCWGG 3 cut(s) 431, 439, 959
PspN4I GGNNCC 5 cut(s) 304, 437, 727, 937, 938
PspPI GGNCC 4 cut(s) 302, 533, 936, 990
PspPPI RGGWCCY 1 cut(s) 936
PstI CTGCAG 1 cut(s) 1243
PsuI RGATCY 2 cut(s) 915, 1196
PvuII CAGCTG 2 cut(s) 682, 967
RseI CAYNNNNRTG 1 cut(s) 170
SacI GAGCTC 1 cut(s) 766
SacII CCGCGG 1 cut(s) 356
SaqAI TTAA 3 cut(s) 119, 588, 1065
SatI GCNGC 5 cut(s) 356, 683, 965, 968, 1239
Sau3AI GATC 4 cut(s) 55, 595, 915, 1196
Sau96I GGNCC 4 cut(s) 302, 533, 936, 990
SchI GAGTC 5 cut(s) 372, 497, 847, 1152, 1171
ScrFI CCNGG 4 cut(s) 49, 433, 441, 961
SduI GDGCHC 4 cut(s) 290, 440, 766, 1193
SfaNI GCATC 2 cut(s) 195, 595
SfcI CTRYAG 2 cut(s) 1123, 1239
Sfr303I CCGCGG 1 cut(s) 356
SgrBI CCGCGG 1 cut(s) 356
SinI GGWCC 3 cut(s) 302, 936, 990
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 3 cut(s) 118, 281, 318
SmoI CTYRAG 3 cut(s) 118, 281, 318
Sse9I AATT 4 cut(s) 689, 701, 746, 791
SsiI CCGC 6 cut(s) 176, 208, 326, 353, 355, 624
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 766
StyD4I CCNGG 4 cut(s) 47, 431, 439, 959
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 5 cut(s) 647, 892, 944, 1012, 1207
TaqI TCGA 4 cut(s) 706, 894, 949, 1229
TasI AATT 4 cut(s) 689, 701, 746, 791
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 4 cut(s) 136, 275, 650, 896
Tru1I TTAA 3 cut(s) 119, 588, 1065
Tru9I TTAA 3 cut(s) 119, 588, 1065
TscAI CASTG 2 cut(s) 337, 383
TseFI GTSAC 5 cut(s) 328, 489, 1090, 1135, 1166
TseI GCWGC 4 cut(s) 682, 964, 967, 1238
Tsp45I GTSAC 5 cut(s) 328, 489, 1090, 1135, 1166
TspDTI ATGAA 7 cut(s) 209, 251, 622, 714, 1034, 1035, 1049
TspRI CASTG 2 cut(s) 337, 383
Vha464I CTTAAG 1 cut(s) 118
VpaK11BI GGWCC 3 cut(s) 302, 936, 990
XapI RAATTY 2 cut(s) 701, 791
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 1 cut(s) 1236
XcmI CCANNNNNNNNNTGG 2 cut(s) 907, 984
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.