Rw2G018750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006385
Physical Location & Seq
Reverse (-)
24525903 .. 24529047
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018750.1

Sequence Viewer

Length: 1194 bp
ATGAAATCAGAATCGGAAATGGAATTGGAATTGAATAAAGAGAAGATTCTATACTCATGTTTCTTTGAGTCGAGTCAATGCGGTGCGGTTGAATACCTGAATCAAGTTATTCGCCCCATCAGATTGAGCCAGGTTAATAGTAATGATGATGCTGATGATATTGCCGTGGAGTTGCGCGAGGCAGCTTATAAGAGGTCCAAGGTTGATTTATGGTATTCCAATTGCAGTCGCTTCGGCTCCAAAATTCTTGTCGCCGGTGGCCATATTTATTGGTTTGACACGACCAGGGGGAAATTCGAGGTTTCCTCCCCAACGAAAACCAAAATCAAGAGCTTCTCAACAGGTAAACTATCCGCGCTGTTGGGGGAGCTGAACCGCAAACTCTATTGCATCGACAACAACCCCTTTAGCATGACTCCTCCCTCTCAGCCTTTTCATGCACGGCGGTGCTCTGAATACTCCTCCCCTTTTCAGGTATTGGACCCCGAAAACGACCTCAAGTGGCGGCCTCTGGAAGCCCCTCCCTTTATCCGCGGCCAAGAGTCGGAGGCCTTCACTGCGTGTTTTTTGGAGGGGAGCAACAAAATCTTAGGTTGGGTGTGGCATCAGCCTGGAGTGTTTTGCTTTGATGACACCAAGCCTGAAAATGGATGGATAGAGTCACCTTCTTGTCTGGGAAACAGCCTTCCCTTTCTTGACTATGGGCCTTTCTTCGTCCGCCACCATCATCCCAATCTTGGTGCTTTCAACTTAATGTTTAACTATGAACCTGACGATGTACCTCTCCTTGTATCCGCCTATTTCATGCCGAACAACTGTGATTCTTTCCATCCTATCAACAAACCTCTTCAGCTGCCAAAATTGTGTTATGAATTTCGAGGGAATGAGTATGATGACACCGTCTCTACCGATTTTCAATTTGTCCATCTCGGAGCTCAAAAAGTCTGCCTTGTTTTGCACAAATTCTTCTACCCATCAACAAGGAAGGCTCAATGTGGAAACATGGGGAGTCTTCTTCTCATAACATTTGAATATGACATCATTAGTAATACTACTGTCGACTCCAAATCCACCTTGGAGATCCAGACCCGATTGCTCGGGACCCACCGCCGTCGATACTTTACCAGGCAGCTGCGTAAGCCAGATGCTACATGGACCAACACAGCATGCCTGACTGGTGCATTTCTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

397

Amino Acids

45.5

Weight (kDa)

6.38

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 71 - 248 9.1e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 189
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 3 cut(s) 177, 356, 534
AclWI GGATC 1 cut(s) 1075
AcoI YGGCCR 2 cut(s) 259, 535
AcsI RAATTY 4 cut(s) 243, 293, 872, 962
AcuI CTGAAG 1 cut(s) 833
AdeI CACNNNGTG 1 cut(s) 561
AfaI GTAC 1 cut(s) 780
AfiI CCNNNNNNNGG 4 cut(s) 472, 544, 647, 737
AgsI TTSAA 5 cut(s) 34, 92, 748, 917, 1031
AjnI CCWGG 4 cut(s) 129, 284, 610, 1124
AleI CACNNNNGTG 1 cut(s) 445
AluBI AGCT 6 cut(s) 185, 333, 370, 853, 935, 1132
AluI AGCT 6 cut(s) 185, 333, 370, 853, 935, 1132
Alw21I GWGCWC 2 cut(s) 452, 937
Alw26I GTCTC 1 cut(s) 907
AlwI GGATC 1 cut(s) 1075
Ama87I CYCGRG 1 cut(s) 1097
AoxI GGCC 5 cut(s) 259, 506, 535, 549, 704
ApeKI GCWGC 4 cut(s) 182, 853, 1129, 1132
ApoI RAATTY 4 cut(s) 243, 293, 872, 962
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
AspLEI GCGC 2 cut(s) 177, 358
AspS9I GGNCC 5 cut(s) 195, 481, 704, 1101, 1155
AsuHPI GGTGA 1 cut(s) 654
AvaI CYCGRG 1 cut(s) 1097
AvaII GGWCC 4 cut(s) 195, 481, 1101, 1155
BalI TGGCCA 1 cut(s) 261
BanII GRGCYC 1 cut(s) 937
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
BbsI GAAGAC 1 cut(s) 1004
Bbv12I GWGCWC 2 cut(s) 452, 937
BbvI GCAGC 4 cut(s) 194, 840, 1119, 1141
BccI CCATC 6 cut(s) 125, 645, 732, 837, 933, 982
BceAI ACGGC 3 cut(s) 149, 458, 1095
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 4 cut(s) 131, 286, 612, 1126
BciVI GTATCC 1 cut(s) 802
BcoDI GTCTC 1 cut(s) 907
BfuI GTATCC 1 cut(s) 802
BisI GCNGC 6 cut(s) 183, 506, 535, 854, 1130, 1133
BlsI GCNGC 6 cut(s) 184, 507, 536, 855, 1131, 1134
Bme1390I CCNGG 4 cut(s) 131, 286, 612, 1126
Bme18I GGWCC 4 cut(s) 195, 481, 1101, 1155
BmeT110I CYCGRG 1 cut(s) 1097
BmgT120I GGNCC 5 cut(s) 195, 481, 704, 1101, 1155
BmiI GGNNCC 4 cut(s) 238, 483, 1102, 1103
BmrFI CCNGG 4 cut(s) 131, 286, 612, 1126
BmsI GCATC 4 cut(s) 139, 399, 613, 1135
BpiI GAAGAC 1 cut(s) 1004
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 482
BsaJI CCNNGG 5 cut(s) 165, 198, 285, 532, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 472, 544, 647, 737
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 1180
BseBI CCWGG 4 cut(s) 131, 286, 612, 1126
BseDI CCNNGG 5 cut(s) 165, 198, 285, 532, 1074
BseGI GGATG 3 cut(s) 656, 727, 829
BseLI CCNNNNNNNGG 4 cut(s) 472, 544, 647, 737
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 1180
BseRI GAGGAG 2 cut(s) 408, 451
BseXI GCAGC 4 cut(s) 194, 840, 1119, 1141
Bsh1236I CGCG 3 cut(s) 177, 356, 534
BshFI GGCC 5 cut(s) 261, 508, 537, 551, 706
BsiHKAI GWGCWC 2 cut(s) 452, 937
BsiHKCI CYCGRG 1 cut(s) 1097
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 1114
BslI CCNNNNNNNGG 4 cut(s) 472, 544, 647, 737
BsmAI GTCTC 1 cut(s) 907
BsmBI CGTCTC 1 cut(s) 907
BsmFI GGGAC 1 cut(s) 1114
BsnI GGCC 5 cut(s) 261, 508, 537, 551, 706
BsoBI CYCGRG 1 cut(s) 1097
Bsp1286I GDGCHC 2 cut(s) 452, 937
Bsp143I GATC 2 cut(s) 1080, 1188
BspANI GGCC 5 cut(s) 261, 508, 537, 551, 706
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 3 cut(s) 177, 356, 534
BspLI GGNNCC 4 cut(s) 238, 483, 1102, 1103
BspPI GGATC 1 cut(s) 1075
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 1 cut(s) 1180
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 5 cut(s) 165, 198, 285, 532, 1074
BssMI GATC 2 cut(s) 1080, 1188
BssT1I CCWWGG 2 cut(s) 198, 1074
Bst2UI CCWGG 4 cut(s) 131, 286, 612, 1126
Bst4CI ACNGT 3 cut(s) 818, 901, 1057
Bst6I CTCTTC 1 cut(s) 852
BstC8I GCNNGC 1 cut(s) 1168
BstDEI CTNAG 2 cut(s) 426, 589
BstDSI CCRYGG 2 cut(s) 165, 532
BstF5I GGATG 3 cut(s) 656, 727, 829
BstFNI CGCG 3 cut(s) 177, 356, 534
BstHHI GCGC 2 cut(s) 177, 358
BstKTI GATC 2 cut(s) 1083, 1191
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 2 cut(s) 1080, 1188
BstMWI GCNNNNNNNGC 2 cut(s) 557, 1138
BstNI CCWGG 4 cut(s) 131, 286, 612, 1126
BstNSI RCATGY 1 cut(s) 1170
BstSCI CCNGG 4 cut(s) 129, 284, 610, 1124
BstUI CGCG 3 cut(s) 177, 356, 534
BstV1I GCAGC 4 cut(s) 194, 840, 1119, 1141
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 1 cut(s) 1080
BstYI RGATCY 1 cut(s) 1080
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 5 cut(s) 261, 508, 537, 551, 706
BtgI CCRYGG 2 cut(s) 165, 532
BtsCI GGATG 3 cut(s) 656, 727, 829
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 1 cut(s) 1168
CfoI GCGC 2 cut(s) 177, 358
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 5 cut(s) 195, 481, 704, 1101, 1155
Cfr42I CCGCGG 1 cut(s) 535
Csp6I GTAC 1 cut(s) 779
CviAII CATG 7 cut(s) 57, 412, 437, 805, 1003, 1152, 1167
CviQI GTAC 1 cut(s) 779
DdeI CTNAG 2 cut(s) 426, 589
DpnI GATC 2 cut(s) 1082, 1190
DpnII GATC 2 cut(s) 1080, 1188
DraIII CACNNNGTG 1 cut(s) 561
EaeI YGGCCR 2 cut(s) 259, 535
Eam1104I CTCTTC 1 cut(s) 852
EarI CTCTTC 1 cut(s) 852
EciI GGCGGA 2 cut(s) 707, 784
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 2 cut(s) 198, 1074
Eco147I AGGCCT 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 937
Eco47I GGWCC 4 cut(s) 195, 481, 1101, 1155
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 1097
EcoICRI GAGCTC 1 cut(s) 935
EcoO109I RGGNCCY 1 cut(s) 1101
EcoRII CCWGG 4 cut(s) 129, 284, 610, 1124
EcoT14I CCWWGG 2 cut(s) 198, 1074
EcoT38I GRGCYC 1 cut(s) 937
ErhI CCWWGG 2 cut(s) 198, 1074
Esp3I CGTCTC 1 cut(s) 907
FaeI CATG 7 cut(s) 60, 415, 440, 808, 1006, 1155, 1170
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 1 cut(s) 1114
FatI CATG 7 cut(s) 56, 411, 436, 804, 1002, 1151, 1166
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 6 cut(s) 183, 506, 535, 854, 1130, 1133
FokI GGATG 3 cut(s) 663, 714, 816
FriOI GRGCYC 1 cut(s) 937
Fsp4HI GCNGC 6 cut(s) 183, 506, 535, 854, 1130, 1133
GlaI GCGC 2 cut(s) 176, 357
GluI GCNGC 6 cut(s) 183, 506, 535, 854, 1130, 1133
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 5 cut(s) 261, 508, 537, 551, 706
HapII CCGG 1 cut(s) 255
HhaI GCGC 2 cut(s) 177, 358
Hin1II CATG 7 cut(s) 60, 415, 440, 808, 1006, 1155, 1170
Hin6I GCGC 2 cut(s) 175, 356
HinP1I GCGC 2 cut(s) 175, 356
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HpaII CCGG 1 cut(s) 255
HphI GGTGA 1 cut(s) 654
Hpy166II GTNNAC 2 cut(s) 347, 1060
Hpy188I TCNGA 7 cut(s) 10, 16, 122, 454, 547, 932, 1188
Hpy188III TCNNGA 5 cut(s) 328, 512, 695, 1084, 1099
Hpy8I GTNNAC 2 cut(s) 347, 1060
Hpy99I CGWCG 1 cut(s) 1116
HpyAV CCTTC 4 cut(s) 562, 675, 695, 979
HpyCH4III ACNGT 3 cut(s) 818, 901, 1057
HpyCH4V TGCA 5 cut(s) 225, 390, 440, 958, 1181
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 1138
HpyF3I CTNAG 2 cut(s) 426, 589
Hsp92II CATG 7 cut(s) 60, 415, 440, 808, 1006, 1155, 1170
HspAI GCGC 2 cut(s) 175, 356
KflI GGGWCCC 1 cut(s) 1101
KspI CCGCGG 1 cut(s) 535
Kzo9I GATC 2 cut(s) 1080, 1188
LmnI GCTCC 4 cut(s) 242, 367, 576, 932
Lsp1109I GCAGC 4 cut(s) 194, 840, 1119, 1141
LweI GCATC 4 cut(s) 139, 399, 613, 1135
MaeIII GTNAC 1 cut(s) 660
MalI GATC 2 cut(s) 1082, 1190
MboI GATC 2 cut(s) 1080, 1188
MboII GAAGA 6 cut(s) 55, 703, 839, 958, 1004, 1007
MfeI CAATTG 1 cut(s) 220
MflI RGATCY 1 cut(s) 1080
MhlI GDGCHC 2 cut(s) 452, 937
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 9 cut(s) 23, 29, 220, 243, 293, 860, 872, 917, 962
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 7 cut(s) 77, 82, 409, 551, 668, 1018, 1055
MmeI TCCRAC 1 cut(s) 525
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 3 cut(s) 135, 752, 759
MslI CAYNNNNRTG 1 cut(s) 445
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 3 cut(s) 534, 853, 1132
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 4 cut(s) 131, 286, 612, 1126
MunI CAATTG 1 cut(s) 220
MvaI CCWGG 4 cut(s) 131, 286, 612, 1126
MvnI CGCG 3 cut(s) 177, 356, 534
MwoI GCNNNNNNNGC 2 cut(s) 557, 1138
NdeII GATC 2 cut(s) 1080, 1188
NlaIII CATG 7 cut(s) 60, 415, 440, 808, 1006, 1155, 1170
NlaIV GGNNCC 4 cut(s) 238, 483, 1102, 1103
NmuCI GTSAC 1 cut(s) 660
NspI RCATGY 1 cut(s) 1170
OliI CACNNNNGTG 1 cut(s) 445
PaeI GCATGC 1 cut(s) 1170
PceI AGGCCT 1 cut(s) 551
PfeI GAWTC 4 cut(s) 11, 46, 100, 821
PflFI GACNNNGTC 1 cut(s) 899
PkrI GCNGC 6 cut(s) 184, 507, 536, 855, 1131, 1134
PleI GAGTC 7 cut(s) 76, 81, 409, 550, 667, 1017, 1055
PpsI GAGTC 7 cut(s) 76, 81, 409, 550, 667, 1017, 1055
PpuMI RGGWCCY 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 937
Psp5II RGGWCCY 1 cut(s) 1101
Psp6I CCWGG 4 cut(s) 129, 284, 610, 1124
PspGI CCWGG 4 cut(s) 129, 284, 610, 1124
PspN4I GGNNCC 4 cut(s) 238, 483, 1102, 1103
PspPI GGNCC 5 cut(s) 195, 481, 704, 1101, 1155
PspPPI RGGWCCY 1 cut(s) 1101
PsuI RGATCY 1 cut(s) 1080
PsyI GACNNNGTC 1 cut(s) 899
PvuII CAGCTG 2 cut(s) 853, 1132
RsaI GTAC 1 cut(s) 780
RsaNI GTAC 1 cut(s) 779
RseI CAYNNNNRTG 1 cut(s) 445
SacI GAGCTC 1 cut(s) 937
SacII CCGCGG 1 cut(s) 535
SalI GTCGAC 1 cut(s) 1058
SaqAI TTAA 3 cut(s) 135, 752, 759
SatI GCNGC 6 cut(s) 183, 506, 535, 854, 1130, 1133
Sau3AI GATC 2 cut(s) 1080, 1188
Sau96I GGNCC 5 cut(s) 195, 481, 704, 1101, 1155
SchI GAGTC 7 cut(s) 77, 82, 409, 551, 668, 1018, 1055
ScrFI CCNGG 4 cut(s) 131, 286, 612, 1126
SduI GDGCHC 2 cut(s) 452, 937
SfaNI GCATC 4 cut(s) 139, 399, 613, 1135
Sfr303I CCGCGG 1 cut(s) 535
SgrAI CRCCGGYG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 535
SinI GGWCC 4 cut(s) 195, 481, 1101, 1155
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SphI GCATGC 1 cut(s) 1170
Sse9I AATT 9 cut(s) 23, 29, 220, 243, 293, 860, 872, 917, 962
SseBI AGGCCT 1 cut(s) 551
SstI GAGCTC 1 cut(s) 937
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 4 cut(s) 129, 284, 610, 1124
StyI CCWWGG 2 cut(s) 198, 1074
TaaI ACNGT 3 cut(s) 818, 901, 1057
TaqI TCGA 6 cut(s) 71, 297, 393, 877, 1059, 1114
TasI AATT 9 cut(s) 23, 29, 220, 243, 293, 860, 872, 917, 962
TauI GCSGC 2 cut(s) 508, 537
TfiI GAWTC 4 cut(s) 11, 46, 100, 821
Tru1I TTAA 3 cut(s) 135, 752, 759
Tru9I TTAA 3 cut(s) 135, 752, 759
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 1 cut(s) 660
TseI GCWGC 4 cut(s) 182, 853, 1129, 1132
Tsp45I GTSAC 1 cut(s) 660
TspDTI ATGAA 5 cut(s) 17, 425, 780, 793, 885
TspRI CASTG 1 cut(s) 562
Tth111I GACNNNGTC 1 cut(s) 899
VpaK11BI GGWCC 4 cut(s) 195, 481, 1101, 1155
XapI RAATTY 4 cut(s) 243, 293, 872, 962
XceI RCATGY 1 cut(s) 1170
XcmI CCANNNNNNNNNTGG 2 cut(s) 1072, 1149
XmiI GTMKAC 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.