Rroxscaffold_4G00293450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003061
Physical Location & Seq
Forward (+)
13727970 .. 13728761
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293450.1

Sequence Viewer

Length: 792 bp
ATGGACCACCACCTTGTCAACCTCTCCGGCGCCGGCCGCCGGACCTCTGATCGGACATGGGAAGATTTACCCAAAGACTGTTTGCTCAATATCTTGGAGAGAGTCGGAACAGAGTCTTTACTAATGTTCGTCCCTTTGGTCTGCAAATCTTGGTACAGAGCAAGCCTCAGTTCTTCTTGCTGGAAACGCCTCTTTTTCCCGGCGGAGATTGAAAAAGGTACTGTCATTTATCCTTGGAGCTTTCCCGTTTCTACTTCCGACGACGATGATCAGAGCACTAGTTTCGATTACGTTATCAGAAGATATGCAAAGGAGTTTGGGATTGAATGGAGTCGTTTCTATGTTTCTAAGTTCATCAAGTTTGCGGTTAATCGTAGCCAAGGGGAGACCCAGTTTCTCAAGGTACCCGGAATTTGCAGCCACTATATGAATGCAATGAGAGAGATACTAGAACACATCGGGAGGAATTGTAGAGGTTTTGAGGGTTTGCATATGTCTCATGCATTGGTTGATGCGGATTTGGCTGAGGACATTGTGAAATATGTGCCACAGATTAAGTACTTGTGCTTGAAGAAGGCCAAGATTGGCCGGATTGAGCTTATTGTGTTGATCAAGGGGTGCAGGGAGCTTAAGGTTTTGGATGTTAAGGACTGTGTGGGTTTTACTGAGGGTGATAGGGAAATAGCAGAGCTTGGTTCTCACATTGGAGAGTTTATGATGGAGGGTTCGACACAATTCAGGAGACGAGATTGGGTTGAGGTTGCTGGGGTTGTTCAGGTTGATGAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

30.26

Weight (kDa)

6.02

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 20 - 66 5.4e-08 F-box-like
F-box PF00646 20 - 62 1.4e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 2 cut(s) 29, 403
AciI CCGC 4 cut(s) 37, 203, 365, 515
AcoI YGGCCR 2 cut(s) 34, 586
AcsI RAATTY 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 4 cut(s) 155, 220, 405, 560
AfiI CCNNNNNNNGG 2 cut(s) 39, 51
AflII CTTAAG 1 cut(s) 629
AgsI TTSAA 3 cut(s) 212, 326, 571
AhlI ACTAGT 1 cut(s) 278
AluBI AGCT 4 cut(s) 240, 598, 628, 691
AluI AGCT 4 cut(s) 240, 598, 628, 691
Alw21I GWGCWC 1 cut(s) 278
Alw26I GTCTC 3 cut(s) 380, 501, 736
AoxI GGCC 3 cut(s) 34, 576, 586
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 1 cut(s) 411
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 4, 42
AsuC2I CCSGG 2 cut(s) 200, 408
AsuHPI GGTGA 1 cut(s) 683
AvaII GGWCC 2 cut(s) 4, 42
BanI GGYRCC 2 cut(s) 29, 403
Bbv12I GWGCWC 1 cut(s) 278
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 712
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BclI TGATCA 2 cut(s) 268, 609
BcnI CCSGG 2 cut(s) 200, 408
BcoDI GTCTC 3 cut(s) 380, 501, 736
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 2 cut(s) 279, 449
BfoI RGCGCY 1 cut(s) 33
BfrI CTTAAG 1 cut(s) 629
BisI GCNGC 2 cut(s) 37, 418
BlsI GCNGC 2 cut(s) 38, 419
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 2 cut(s) 200, 408
Bme18I GGWCC 2 cut(s) 4, 42
BmgT120I GGNCC 2 cut(s) 4, 42
BmiI GGNNCC 2 cut(s) 31, 405
BmrFI CCNGG 2 cut(s) 200, 408
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 385
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 200, 408
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 233, 379
BsaXI ACNNNNNCTCC 4 cut(s) 377, 407, 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 51
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 441
BseDI CCNNGG 2 cut(s) 233, 379
BseGI GGATG 1 cut(s) 646
BseLI CCNNNNNNNGG 2 cut(s) 39, 51
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 181, 516, 657
BseNI ACTGG 1 cut(s) 391
BseX3I CGGCCG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 764
BsgI GTGCAG 1 cut(s) 640
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 36, 578, 588
BshNI GGYRCC 2 cut(s) 29, 403
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 6 cut(s) 27, 33, 40, 200, 408, 589
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 39, 51
BsmAI GTCTC 3 cut(s) 380, 501, 736
BsmBI CGTCTC 1 cut(s) 736
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 3 cut(s) 36, 578, 588
Bso31I GGTCTC 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 278
Bsp143I GATC 3 cut(s) 49, 268, 609
BspACI CCGC 4 cut(s) 37, 203, 365, 515
BspANI GGCC 3 cut(s) 36, 578, 588
BspCNI CTCAG 3 cut(s) 180, 517, 658
BspLI GGNNCC 2 cut(s) 31, 405
BspT107I GGYRCC 2 cut(s) 29, 403
BspTI CTTAAG 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 233, 379
BssMI GATC 3 cut(s) 49, 268, 609
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 233, 379
Bst4CI ACNGT 3 cut(s) 80, 223, 653
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 34, 163
BstDEI CTNAG 4 cut(s) 167, 348, 525, 666
BstF5I GGATG 1 cut(s) 646
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 3 cut(s) 52, 271, 612
BstMAI GTCTC 3 cut(s) 380, 501, 736
BstMBI GATC 3 cut(s) 49, 268, 609
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 3 cut(s) 36, 186, 521
BstSCI CCNGG 2 cut(s) 198, 406
BstV1I GCAGC 1 cut(s) 429
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 3 cut(s) 36, 578, 588
BtsCI GGATG 1 cut(s) 646
Cac8I GCNNGC 2 cut(s) 34, 163
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 4, 42
Csp6I GTAC 4 cut(s) 154, 219, 404, 559
CviAII CATG 2 cut(s) 57, 500
CviQI GTAC 4 cut(s) 154, 219, 404, 559
DdeI CTNAG 4 cut(s) 167, 348, 525, 666
DinI GGCGCC 1 cut(s) 31
DpnI GATC 3 cut(s) 51, 270, 611
DpnII GATC 3 cut(s) 49, 268, 609
EaeI YGGCCR 2 cut(s) 34, 586
EagI CGGCCG 1 cut(s) 34
EciI GGCGGA 1 cut(s) 218
EclXI CGGCCG 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 233, 379
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 4, 42
Eco52I CGGCCG 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 233, 379
EcoT22I ATGCAT 1 cut(s) 505
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 2 cut(s) 233, 379
Esp3I CGTCTC 1 cut(s) 736
FaeI CATG 2 cut(s) 60, 503
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 56, 499
FauNDI CATATG 1 cut(s) 492
FbaI TGATCA 2 cut(s) 268, 609
Fnu4HI GCNGC 2 cut(s) 37, 418
FokI GGATG 1 cut(s) 653
Fsp4HI GCNGC 2 cut(s) 37, 418
FspBI CTAG 2 cut(s) 279, 449
GlaI GCGC 1 cut(s) 31
GluI GCNGC 2 cut(s) 37, 418
GsaI CCCAGC 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 3 cut(s) 36, 578, 588
HapII CCGG 6 cut(s) 27, 33, 40, 200, 408, 589
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 2 cut(s) 60, 503
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 3 cut(s) 102, 113, 331
HpaII CCGG 6 cut(s) 27, 33, 40, 200, 408, 589
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 6 cut(s) 49, 54, 107, 259, 273, 299
Hpy188III TCNNGA 2 cut(s) 460, 739
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 2 cut(s) 263, 266
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 3 cut(s) 80, 223, 653
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 7 cut(s) 144, 308, 417, 434, 490, 503, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 186, 521
HpyF3I CTNAG 4 cut(s) 167, 348, 525, 666
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 2 cut(s) 60, 503
HspAI GCGC 1 cut(s) 30
KasI GGCGCC 1 cut(s) 29
KpnI GGTACC 1 cut(s) 407
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Ksp22I TGATCA 2 cut(s) 268, 609
Kzo9I GATC 3 cut(s) 49, 268, 609
LmnI GCTCC 2 cut(s) 237, 625
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 1 cut(s) 502
MaeI CTAG 2 cut(s) 279, 449
MaeII ACGT 1 cut(s) 291
MalI GATC 3 cut(s) 51, 270, 611
MboI GATC 3 cut(s) 49, 268, 609
MboII GAAGA 4 cut(s) 74, 165, 312, 583
MhlI GDGCHC 1 cut(s) 278
MluCI AATT 3 cut(s) 411, 466, 734
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 3 cut(s) 111, 122, 340
MmeI TCCRAC 2 cut(s) 85, 282
Mph1103I ATGCAT 1 cut(s) 505
MroNI GCCGGC 1 cut(s) 32
MseI TTAA 4 cut(s) 369, 555, 630, 645
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 6 cut(s) 27, 33, 40, 200, 408, 589
MspR9I CCNGG 2 cut(s) 200, 408
Mva1269I GAATGC 1 cut(s) 436
MwoI GCNNNNNNNGC 3 cut(s) 36, 186, 521
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 2 cut(s) 200, 408
NdeI CATATG 1 cut(s) 492
NdeII GATC 3 cut(s) 49, 268, 609
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 2 cut(s) 60, 503
NlaIV GGNNCC 2 cut(s) 31, 405
NsiI ATGCAT 1 cut(s) 505
PctI GAATGC 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 34
PkrI GCNGC 2 cut(s) 38, 419
PleI GAGTC 3 cut(s) 110, 121, 339
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 3 cut(s) 110, 121, 339
PspFI CCCAGC 1 cut(s) 764
PspN4I GGNNCC 2 cut(s) 31, 405
PspPI GGNCC 2 cut(s) 4, 42
RsaI GTAC 4 cut(s) 155, 220, 405, 560
RsaNI GTAC 4 cut(s) 154, 219, 404, 559
SaqAI TTAA 4 cut(s) 369, 555, 630, 645
SatI GCNGC 2 cut(s) 37, 418
Sau3AI GATC 3 cut(s) 49, 268, 609
Sau96I GGNCC 2 cut(s) 4, 42
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 3 cut(s) 111, 122, 340
ScrFI CCNGG 2 cut(s) 200, 408
SduI GDGCHC 1 cut(s) 278
SfaNI GCATC 1 cut(s) 502
SfoI GGCGCC 1 cut(s) 31
SinI GGWCC 2 cut(s) 4, 42
SmlI CTYRAG 2 cut(s) 398, 629
SmoI CTYRAG 2 cut(s) 398, 629
SpeI ACTAGT 1 cut(s) 278
Sse9I AATT 3 cut(s) 411, 466, 734
SsiI CCGC 4 cut(s) 37, 203, 365, 515
SspDI GGCGCC 1 cut(s) 29
SspMI CTAG 2 cut(s) 279, 449
StyD4I CCNGG 2 cut(s) 198, 406
StyI CCWWGG 2 cut(s) 233, 379
TaaI ACNGT 3 cut(s) 80, 223, 653
TaiI ACGT 1 cut(s) 294
TaqI TCGA 2 cut(s) 285, 728
TasI AATT 3 cut(s) 411, 466, 734
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 4 cut(s) 369, 555, 630, 645
Tru9I TTAA 4 cut(s) 369, 555, 630, 645
TseI GCWGC 1 cut(s) 417
TspDTI ATGAA 2 cut(s) 343, 443
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 4, 42
XapI RAATTY 1 cut(s) 411
XspI CTAG 2 cut(s) 279, 449
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.