Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0003061

Physical Location & Seq

Genomic Coordinates Forward (+)

42033475 .. 42038266

Gene Structure 4792 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0304700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGTTGGAGAAGATTGGAGAGTTCAACATGGAGAAGGTCATAGAGGATTTTGAAGCAATGACAAGTGATGCGGAGAGGATCCAGAAGGAGACCCTCAAGAAGATTCTTGAACAAAACAGATCCGCGGAGTACTTGCAGCTTCTGGGACTCAATGGAAGAACTGACCTGGAGAGTTTCAAGAGGGTTGTTCCTCTTGTTACTCACACGGATTTGGACCCTTATATTCAGAGAATTGCAGATGGTGATTCCTCTCCAGTTCTCACTGGAAAACCAATCACAACAATCTCACTGAGTTCTGGTACTACTCAAGGAAGGTCAAAGTTTATACCTTTCAATGATGAACTGATGGAAAATACCATGCAGATATATCAAACCTCTTTTGCCTTCAGAAACAGGGAGTTTCCTATTGGGAATGGGAAGGCCTTACAGTTCATCTATGGAAGCAAGCAGTTCCAAACTAAAGGTGGTTTGGCAGCTGGAACTGCCACCACCAATGTTTACCGTTGTTCAAAGTTCAAGGAGACAATGAAGGCAATGCAGACCCCTTGCTGCAGCCCTGATGAAGTAATATTTGGTCTTGAGTTCCACCAATCCCTTTATTGCCATCTGTTATGTGGACTGTTATTCAGTGATGAAGTTCAGTTGGTATCCTCAACATTTGCTCACAGCATAGTCCAAGCCTTCAGAACCTTTGAGCTATTGTGGGAGGAACTATGTGCTGACATTCGAGATGGCAGTCTCAGCAGCCGGATCACAGTCCCATCAATCCGTGCAGCCATGTTGAAATTACTCGAGCCAAACCCGGAATTGGCTGATTCGGTTTACAGAAAATGTTCAGGACTGAGTAACTGGTATGGACTGATACCAGCCCTTTTTCCAAATGCCAAGTACATATATGGTGTTATGACTGGGTCAATGGAGCTTTATTTGAACAAGCTGAGGCACTATGCAGCGGACTTACCTTTGTTGAGTGCTGATTATGGTGCTTCTGAAGGTTGGATTGCAGCAAATGTCAACCCAAAGGTACCAACTGAGATGACCACTTATGTTGTGCTCCCTAATATTGGCTACTTTGAATTCATCCCTGTTAGGGATGGCATTGATTTCAAGCCAGTGGGTCTTACTGAAGTGAAAATTGGTGAAGAGTATGAGGTTGTGGTCACTAATTTTGCAGGTTTGTACCGATATAGACTAGGAGATGTAGTGAAGGTGATGGGGTTCCATAACTCGACACCTGAGCTCAAATTCCTCTGCCGTAGCAATCTTCTGCTCACCATCAACATTGACAAGAACACCGAGAAAGATTTGCAGATATCTGTTGAAGAAGCAGCCAAGCTTTTAGCTGCAGAAAAATTAGAAGTTGTTGATTTTTCAAGCCATGTAGACTTGTCCACAAACCCTGGTCACTATGTGATCTTCTGGGAACTTAGTGGTGAAGCATCCCAAGAGGTTCTCAATGAATGCTGCAACTGTTTGGACAAGTCTTTTGTGGATGCAGGCTACAGGAGCTCAAGGAAAGTCAACGGAATAGGTCCACTCGAGCTCCGAATTCTTCGGAAGGGAACTTTCCAAAAGGTTCTCAACCATTACCTTGCACAAGGATCTGCTGTTAATCAGTTCAAGACCCCAAGATGTGTAGGGGTTAACAACAGCAAGGTTTTGCAAATCCTATGTGGCAATGTAGTTAGGAGCTTCACTAGTACTGCTTATGATATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like

Protein Analysis

572

Amino Acids

64.01

Weight (kDa)

5.76

Isoelectric Point (pI)

28.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	14 - 322	8e-111	GH3 family N-terminal domain
GH3_M	PF23571	349 - 419	3.9e-28	GH3 family central domain
GH3_C	PF23572	435 - 546	8.2e-34	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003061)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g12570 FvH4_3g12570 FvH4_6g07650 FvH4_7g19271 FvH4_7g19272
malus_domestica	MD07G1173200.v1.1
prunus_persica	Prupe.2G212400_v2.0.a1 Prupe.5G001500_v2.0.a1
pyrus_communis	pycom07g16790
rosa_chinensis	RchiOBHm_Chr1g0363121 RchiOBHm_Chr1g0363131 RchiOBHm_Chr1g0363141
rosa_laevigata	RLG00000027589 RLG00000027591 RLG00000027592
rosa_multiflora	Rmu_co8316487.1_g000001 Rmu_sc0004472.1_g000010 Rmu_sc0005586.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293420 Rroxscaffold_4G00293430 Rroxscaffold_4G00293450
rosa_rugosa	Rorug01G0304700 Rorug01G0304700 Rorug01G0304800 Rorug01G0304900 Rorug01G0305000
rosa_samantha	Rh1AG313600 Rh1AG313700 Rh1BG277000 Rh1BG277100 Rh1BG277200 Rh1CG293600 Rh1CG293700 Rh1DG307600 Rh1DG307700 Rh1DG307800
rosa_wichuraiana	Rw1G027790 Rw1G027800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1163
Acc65I	GGTACC	1 cut(s)	1024
AccB1I	GGYRCC	1 cut(s)	1024
AccI	GTMKAC	1 cut(s)	1383
AccII	CGCG	1 cut(s)	125
AciI	CCGC	4 cut(s)	71, 123, 125, 953
AclWI	GGATC	5 cut(s)	73, 86, 114, 758, 1609
AcsI	RAATTY	3 cut(s)	1076, 1244, 1548
AcuI	CTGAAG	4 cut(s)	370, 667, 1011, 1146
AdeI	CACNNNGTG	1 cut(s)	1411
AfaI	GTAC	6 cut(s)	131, 301, 890, 1026, 1181, 1702
AfiI	CCNNNNNNNGG	3 cut(s)	808, 1064, 1090
AhlI	ACTAGT	1 cut(s)	1697
AjnI	CCWGG	2 cut(s)	165, 1399
AjuI	GAANNNNNNNTTGG	4 cut(s)	555, 587, 1119, 1151
Alw21I	GWGCWC	4 cut(s)	1056, 1242, 1511, 1545
Alw26I	GTCTC	3 cut(s)	83, 515, 743
AlwI	GGATC	5 cut(s)	73, 86, 114, 758, 1609
AlwNI	CAGNNNCTG	1 cut(s)	142
Ama87I	CYCGRG	2 cut(s)	791, 1538
AoxI	GGCC	1 cut(s)	420
ApoI	RAATTY	3 cut(s)	1076, 1244, 1548
ArsI	GACNNNNNNTTYG	2 cut(s)	1469, 1501
Asp700I	GAANNNNTTC	1 cut(s)	832
Asp718I	GGTACC	1 cut(s)	1024
AspS9I	GGNCC	2 cut(s)	214, 1532
AsuC2I	CCSGG	1 cut(s)	803
AsuHPI	GGTGA	5 cut(s)	254, 1151, 1222, 1264, 1445
AvaI	CYCGRG	2 cut(s)	791, 1538
AvaII	GGWCC	2 cut(s)	214, 1532
BamHI	GGATCC	1 cut(s)	78
BanI	GGYRCC	1 cut(s)	1024
BanII	GRGCYC	3 cut(s)	1242, 1511, 1545
Bbv12I	GWGCWC	4 cut(s)	1056, 1242, 1511, 1545
BbvCI	CCTCAGC	1 cut(s)	938
BccI	CCATC	8 cut(s)	233, 340, 612, 725, 769, 1088, 1207, 1283
BceAI	ACGGC	1 cut(s)	1239
BciT130I	CCWGG	2 cut(s)	167, 1401
BciVI	GTATCC	1 cut(s)	658
BcnI	CCSGG	1 cut(s)	803
BcoDI	GTCTC	3 cut(s)	83, 515, 743
BcuI	ACTAGT	1 cut(s)	1697
BfaI	CTAG	2 cut(s)	1193, 1698
BfmI	CTRYAG	3 cut(s)	550, 1344, 1501
BfuAI	ACCTGC	1 cut(s)	1163
BfuI	GTATCC	1 cut(s)	658
BmcAI	AGTACT	2 cut(s)	131, 1702
Bme1390I	CCNGG	3 cut(s)	167, 803, 1401
Bme18I	GGWCC	2 cut(s)	214, 1532
BmeT110I	CYCGRG	2 cut(s)	791, 1538
BmgT120I	GGNCC	2 cut(s)	214, 1532
BmiI	GGNNCC	4 cut(s)	80, 216, 1026, 1220
BmrFI	CCNGG	3 cut(s)	167, 803, 1401
BmrI	ACTGGG	1 cut(s)	918
BmsI	GCATC	3 cut(s)	58, 1448, 1483
BmuI	ACTGGG	1 cut(s)	918
BpmI	CTGGAG	2 cut(s)	188, 237
Bpu10I	CCTNAGC	2 cut(s)	938, 1236
BpuEI	CTTGAG	4 cut(s)	80, 291, 599, 1495
BpuMI	CCSGG	1 cut(s)	803
BsaI	GGTCTC	1 cut(s)	83
BsaJI	CCNNGG	2 cut(s)	123, 1399
BsaXI	ACNNNNNCTCC	4 cut(s)	1188, 1218, 1527, 1557
Bsc4I	CCNNNNNNNGG	3 cut(s)	808, 1064, 1090
Bse1I	ACTGG	5 cut(s)	254, 268, 854, 913, 1112
Bse3DI	GCAATG	3 cut(s)	63, 540, 1684
BseBI	CCWGG	2 cut(s)	167, 1401
BseDI	CCNNGG	2 cut(s)	123, 1399
BseGI	GGATG	4 cut(s)	1080, 1099, 1439, 1498
BseLI	CCNNNNNNNGG	3 cut(s)	808, 1064, 1090
BseMI	GCAATG	3 cut(s)	63, 540, 1684
BseMII	CTCAG	6 cut(s)	281, 754, 833, 929, 1023, 1227
BseNI	ACTGG	5 cut(s)	254, 268, 854, 913, 1112
BsgI	GTGCAG	1 cut(s)	792
Bsh1236I	CGCG	1 cut(s)	125
BshFI	GGCC	1 cut(s)	422
BshNI	GGYRCC	1 cut(s)	1024
BsiHKAI	GWGCWC	4 cut(s)	1056, 1242, 1511, 1545
BsiHKCI	CYCGRG	2 cut(s)	791, 1538
BsiSI	CCGG	2 cut(s)	748, 803
BslFI	GGGAC	2 cut(s)	159, 743
BslI	CCNNNNNNNGG	3 cut(s)	808, 1064, 1090
BsmAI	GTCTC	3 cut(s)	83, 515, 743
BsmFI	GGGAC	2 cut(s)	159, 743
BsmI	GAATGC	1 cut(s)	1466
BsnI	GGCC	1 cut(s)	422
Bso31I	GGTCTC	1 cut(s)	83
BsoBI	CYCGRG	2 cut(s)	791, 1538
Bsp1286I	GDGCHC	4 cut(s)	1056, 1242, 1511, 1545
Bsp143I	GATC	5 cut(s)	78, 119, 750, 1413, 1601
BspACI	CCGC	4 cut(s)	71, 123, 125, 953
BspANI	GGCC	1 cut(s)	422
BspCNI	CTCAG	6 cut(s)	282, 753, 834, 930, 1024, 1228
BspFNI	CGCG	1 cut(s)	125
BspLI	GGNNCC	4 cut(s)	80, 216, 1026, 1220
BspMAI	CTGCAG	2 cut(s)	554, 1348
BspMI	ACCTGC	1 cut(s)	1163
BspPI	GGATC	5 cut(s)	73, 86, 114, 758, 1609
BspT107I	GGYRCC	1 cut(s)	1024
BspTNI	GGTCTC	1 cut(s)	83
BsrDI	GCAATG	3 cut(s)	63, 540, 1684
BsrI	ACTGG	5 cut(s)	254, 268, 854, 913, 1112
BssECI	CCNNGG	2 cut(s)	123, 1399
BssMI	GATC	5 cut(s)	78, 119, 750, 1413, 1601
Bst2UI	CCWGG	2 cut(s)	167, 1401
Bst4CI	ACNGT	5 cut(s)	429, 503, 621, 757, 1472
Bst6I	CTCTTC	1 cut(s)	1137
BstC8I	GCNNGC	2 cut(s)	446, 1498
BstDEI	CTNAG	7 cut(s)	290, 740, 842, 938, 1032, 1236, 1427
BstDSI	CCRYGG	1 cut(s)	123
BstF5I	GGATG	4 cut(s)	1080, 1099, 1439, 1498
BstFNI	CGCG	1 cut(s)	125
BstKTI	GATC	5 cut(s)	81, 122, 753, 1416, 1604
BstMAI	GTCTC	3 cut(s)	83, 515, 743
BstMBI	GATC	5 cut(s)	78, 119, 750, 1413, 1601
BstMWI	GCNNNNNNNGC	3 cut(s)	482, 741, 1506
BstNI	CCWGG	2 cut(s)	167, 1401
BstSCI	CCNGG	3 cut(s)	165, 801, 1399
BstSFI	CTRYAG	3 cut(s)	550, 1344, 1501
BstUI	CGCG	1 cut(s)	125
BstX2I	RGATCY	3 cut(s)	78, 119, 1601
BstYI	RGATCY	3 cut(s)	78, 119, 1601
BsuI	GTATCC	1 cut(s)	658
BsuRI	GGCC	1 cut(s)	422
BtgI	CCRYGG	1 cut(s)	123
BtsCI	GGATG	4 cut(s)	1080, 1099, 1439, 1498
BtsIMutI	CAGTG	4 cut(s)	261, 287, 634, 1119
BveI	ACCTGC	1 cut(s)	1163
Cac8I	GCNNGC	2 cut(s)	446, 1498
CaiI	CAGNNNCTG	1 cut(s)	142
Cfr13I	GGNCC	2 cut(s)	214, 1532
Cfr42I	CCGCGG	1 cut(s)	126
Csp6I	GTAC	6 cut(s)	130, 300, 889, 1025, 1180, 1701
CspCI	CAANNNNNGTGG	2 cut(s)	1030, 1065
CviAII	CATG	4 cut(s)	28, 358, 778, 1379
CviQI	GTAC	6 cut(s)	130, 300, 889, 1025, 1180, 1701
DdeI	CTNAG	7 cut(s)	290, 740, 842, 938, 1032, 1236, 1427
DpnI	GATC	5 cut(s)	80, 121, 752, 1415, 1603
DpnII	GATC	5 cut(s)	78, 119, 750, 1413, 1601
DraIII	CACNNNGTG	1 cut(s)	1411
Eam1104I	CTCTTC	1 cut(s)	1137
EarI	CTCTTC	1 cut(s)	1137
Ecl136II	GAGCTC	3 cut(s)	1240, 1509, 1543
Eco147I	AGGCCT	1 cut(s)	422
Eco24I	GRGCYC	3 cut(s)	1242, 1511, 1545
Eco31I	GGTCTC	1 cut(s)	83
Eco32I	GATATC	1 cut(s)	1314
Eco47I	GGWCC	2 cut(s)	214, 1532
Eco53kI	GAGCTC	3 cut(s)	1240, 1509, 1543
Eco57I	CTGAAG	4 cut(s)	370, 667, 1011, 1146
Eco88I	CYCGRG	2 cut(s)	791, 1538
EcoICRI	GAGCTC	3 cut(s)	1240, 1509, 1543
EcoRI	GAATTC	2 cut(s)	1076, 1548
EcoRII	CCWGG	2 cut(s)	165, 1399
EcoRV	GATATC	1 cut(s)	1314
EcoT38I	GRGCYC	3 cut(s)	1242, 1511, 1545
FaeI	CATG	4 cut(s)	31, 361, 781, 1382
FaqI	GGGAC	2 cut(s)	159, 743
FatI	CATG	4 cut(s)	27, 357, 777, 1378
FblI	GTMKAC	1 cut(s)	1383
FokI	GGATG	4 cut(s)	1067, 1106, 1426, 1505
FriOI	GRGCYC	3 cut(s)	1242, 1511, 1545
FspBI	CTAG	2 cut(s)	1193, 1698
GsuI	CTGGAG	2 cut(s)	188, 237
HaeIII	GGCC	1 cut(s)	422
HapII	CCGG	2 cut(s)	748, 803
Hin1II	CATG	4 cut(s)	31, 361, 781, 1382
HincII	GTYRAC	3 cut(s)	1015, 1522, 1645
HindII	GTYRAC	3 cut(s)	1015, 1522, 1645
HindIII	AAGCTT	1 cut(s)	1334
HinfI	GANTC	4 cut(s)	103, 147, 245, 815
HpaI	GTTAAC	1 cut(s)	1645
HpaII	CCGG	2 cut(s)	748, 803
HphI	GGTGA	5 cut(s)	254, 1151, 1222, 1264, 1445
Hpy166II	GTNNAC	9 cut(s)	499, 617, 823, 1015, 1384, 1392, 1522, 1535, 1645
Hpy188I	TCNGA	6 cut(s)	228, 389, 686, 991, 1547, 1557
Hpy188III	TCNNGA	8 cut(s)	82, 97, 107, 178, 578, 728, 837, 1621
Hpy8I	GTNNAC	9 cut(s)	499, 617, 823, 1015, 1384, 1392, 1522, 1535, 1645
HpyCH4III	ACNGT	5 cut(s)	429, 503, 621, 757, 1472
HpyF10VI	GCNNNNNNNGC	3 cut(s)	482, 741, 1506
HpyF3I	CTNAG	7 cut(s)	290, 740, 842, 938, 1032, 1236, 1427
Hsp92II	CATG	4 cut(s)	31, 361, 781, 1382
KpnI	GGTACC	1 cut(s)	1028
KspAI	GTTAAC	1 cut(s)	1645
KspI	CCGCGG	1 cut(s)	126
Kzo9I	GATC	5 cut(s)	78, 119, 750, 1413, 1601
LmnI	GCTCC	5 cut(s)	919, 1059, 1506, 1548, 1689
LweI	GCATC	3 cut(s)	58, 1448, 1483
MaeI	CTAG	2 cut(s)	1193, 1698
MaeIII	GTNAC	4 cut(s)	196, 845, 1159, 1403
MalI	GATC	5 cut(s)	80, 121, 752, 1415, 1603
MboI	GATC	5 cut(s)	78, 119, 750, 1413, 1601
MboII	GAAGA	8 cut(s)	22, 112, 168, 1154, 1256, 1334, 1408, 1544
MflI	RGATCY	3 cut(s)	78, 119, 1601
MhlI	GDGCHC	4 cut(s)	1056, 1242, 1511, 1545
MluCI	AATT	9 cut(s)	231, 785, 806, 1076, 1134, 1165, 1244, 1352, 1548
MlyI	GAGTC	1 cut(s)	141
MmeI	TCCRAC	1 cut(s)	977
MroXI	GAANNNNTTC	1 cut(s)	832
MseI	TTAA	2 cut(s)	1611, 1644
MspA1I	CMGCKG	3 cut(s)	125, 476, 953
MspI	CCGG	2 cut(s)	748, 803
MspR9I	CCNGG	3 cut(s)	167, 803, 1401
Mva1269I	GAATGC	1 cut(s)	1466
MvaI	CCWGG	2 cut(s)	167, 1401
MvnI	CGCG	1 cut(s)	125
MwoI	GCNNNNNNNGC	3 cut(s)	482, 741, 1506
NciI	CCSGG	1 cut(s)	803
NdeII	GATC	5 cut(s)	78, 119, 750, 1413, 1601
NlaIII	CATG	4 cut(s)	31, 361, 781, 1382
NlaIV	GGNNCC	4 cut(s)	80, 216, 1026, 1220
NmuCI	GTSAC	2 cut(s)	1159, 1403
PaeR7I	CTCGAG	2 cut(s)	791, 1538
PceI	AGGCCT	1 cut(s)	422
PctI	GAATGC	1 cut(s)	1466
PdmI	GAANNNNTTC	1 cut(s)	832
PfeI	GAWTC	3 cut(s)	103, 245, 815
PflFI	GACNNNGTC	1 cut(s)	910
PleI	GAGTC	1 cut(s)	141
PpsI	GAGTC	1 cut(s)	141
Psp124BI	GAGCTC	3 cut(s)	1242, 1511, 1545
Psp6I	CCWGG	2 cut(s)	165, 1399
PspGI	CCWGG	2 cut(s)	165, 1399
PspN4I	GGNNCC	4 cut(s)	80, 216, 1026, 1220
PspPI	GGNCC	2 cut(s)	214, 1532
PspXI	VCTCGAGB	2 cut(s)	791, 1538
PstI	CTGCAG	2 cut(s)	554, 1348
PstNI	CAGNNNCTG	1 cut(s)	142
PsuI	RGATCY	3 cut(s)	78, 119, 1601
PsyI	GACNNNGTC	1 cut(s)	910
PvuII	CAGCTG	1 cut(s)	476
RsaI	GTAC	6 cut(s)	131, 301, 890, 1026, 1181, 1702
RsaNI	GTAC	6 cut(s)	130, 300, 889, 1025, 1180, 1701
SacI	GAGCTC	3 cut(s)	1242, 1511, 1545
SacII	CCGCGG	1 cut(s)	126
SaqAI	TTAA	2 cut(s)	1611, 1644
Sau3AI	GATC	5 cut(s)	78, 119, 750, 1413, 1601
Sau96I	GGNCC	2 cut(s)	214, 1532
ScaI	AGTACT	2 cut(s)	131, 1702
SchI	GAGTC	1 cut(s)	141
ScrFI	CCNGG	3 cut(s)	167, 803, 1401
SduI	GDGCHC	4 cut(s)	1056, 1242, 1511, 1545
SfaNI	GCATC	3 cut(s)	58, 1448, 1483
SfcI	CTRYAG	3 cut(s)	550, 1344, 1501
Sfr274I	CTCGAG	2 cut(s)	791, 1538
Sfr303I	CCGCGG	1 cut(s)	126
SgrBI	CCGCGG	1 cut(s)	126
SinI	GGWCC	2 cut(s)	214, 1532
SlaI	CTCGAG	2 cut(s)	791, 1538
SmlI	CTYRAG	6 cut(s)	95, 306, 578, 791, 1510, 1538
SmoI	CTYRAG	6 cut(s)	95, 306, 578, 791, 1510, 1538
SpeI	ACTAGT	1 cut(s)	1697
Sse9I	AATT	9 cut(s)	231, 785, 806, 1076, 1134, 1165, 1244, 1352, 1548
SseBI	AGGCCT	1 cut(s)	422
SsiI	CCGC	4 cut(s)	71, 123, 125, 953
SspI	AATATT	2 cut(s)	570, 1063
SspMI	CTAG	2 cut(s)	1193, 1698
SstI	GAGCTC	3 cut(s)	1242, 1511, 1545
StuI	AGGCCT	1 cut(s)	422
StyD4I	CCNGG	3 cut(s)	165, 801, 1399
TaaI	ACNGT	5 cut(s)	429, 503, 621, 757, 1472
TaqI	TCGA	4 cut(s)	727, 792, 1229, 1539
TasI	AATT	9 cut(s)	231, 785, 806, 1076, 1134, 1165, 1244, 1352, 1548
TatI	WGTACW	3 cut(s)	129, 888, 1700
TfiI	GAWTC	3 cut(s)	103, 245, 815
Tru1I	TTAA	2 cut(s)	1611, 1644
Tru9I	TTAA	2 cut(s)	1611, 1644
TscAI	CASTG	4 cut(s)	268, 294, 634, 1119
TseFI	GTSAC	2 cut(s)	1159, 1403
Tsp45I	GTSAC	2 cut(s)	1159, 1403
TspDTI	ATGAA	7 cut(s)	354, 421, 542, 576, 648, 1069, 1473
TspGWI	ACGGA	3 cut(s)	221, 758, 1539
TspRI	CASTG	4 cut(s)	268, 294, 634, 1119
Tth111I	GACNNNGTC	1 cut(s)	910
VpaK11BI	GGWCC	2 cut(s)	214, 1532
XapI	RAATTY	3 cut(s)	1076, 1244, 1548
XcmI	CCANNNNNNNNNTGG	2 cut(s)	461, 611
XhoI	CTCGAG	2 cut(s)	791, 1538
XmiI	GTMKAC	1 cut(s)	1383
XmnI	GAANNNNTTC	1 cut(s)	832
XspI	CTAG	2 cut(s)	1193, 1698
ZrmI	AGTACT	2 cut(s)	131, 1702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.