Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0003061

Physical Location & Seq

Genomic Coordinates Forward (+)

42048749 .. 42053962

Gene Structure 5214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0304900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGGGGGATTCTGGTGGAAAAGTGAGGTTGGTTCGGTGCCCAAAGTGTGAGAATCTTCTTCCTGAGCTAGCTGATTACTCTGTGTATCAGTGTGGTGGTTGTGGTGCTGTTCTCAGAGCAAAAAAGAAAAGGCAAGATGGGGATACTTTGTCAGAGAAGTCGGATGAAGAAACGGTGGGAGGAGTTTTTGCTAAATTGGCTGGTTCAGATGATAAAGGATTATTAGTTTTAAGTGATGTTTCTGATACAGATGTTAAGTCAAGTGAAGGCTCTTTGGTTTCTGATCAAAGGGTTTTAGAGAAGGAAGATGCTGAGATATGTAGGAAGCCAACCAAAGCTGCGACTGATAACTGGGCTGTAGATAATGGCCTTGATATGAGCATGAAAAAAGATGTACCGGGGAATGCAGCAAGAAATGCAATGGGAAGAGGACATGGAGATTTGAGTTCTGAATCTGGTTCCACTAGCTTAAGCCGAAGATCAGCATGGATGGCAGCTTGGCAAGCTGAGGAGAATAGTGCAAAAGAGGGGTTTCGAAGAAATCCAAGAATTGATGTGGAAGGTATGAGGTCATCAACTTCCAATTATCCTGAAGAGGGTCCATCAAATTACCAGTTGGGTTCTTCTCACAGGATTGGGGAAGCGTTGAGGAAATCTAATGACCAAGATGGGGCAACAAGAGTTCTGTCCCTTGAACAAGACCGAGCTGAGCTGCTGAGGAAGCTGGATGAGTTGAGGGATCAACTTAGTCGGTCTTGTGATATGGCTGATAAGTCGAAAGAGAAGGCTCCTCTTGATGGGAGAATGGTTCCTCCAGATCCTTATGGTGGTTCTGACACTTCATATCCAGGTGCTTCTTCAGGACCAAGTAGACCTTCTATGCAGTACTTTGGACCTGATAAACATCTTTCAGGACACCCTCATTTTAATCAATATCCTGAGCATTTTCCCTACACCAATGGCCATGAAACTGCTATGCCTAGCTTCACTCCTTCCATGCATAACTCAAACAGTTTTTCGGGTTATGGGGATCCTTACGGACCCCAAATGCTCAGTGGAGCTCCACATCCATTTCCTAGGCAATACCAACAGCCATCTCATCCATACTTTTCTGGACAGTATGCTGAAAATCATCCTGATCCATATGAGCTACATCCACATAATGCAATGCTTCACCAACCTTCTTGCCCTTGTTACTATTGCTATGAAAAACACAGGCGGTCATCAGCACCAGTTCCACCCACTGCTTTTCAAAATAAAAGCTTCCCTGACTTCCCAAGCAATCCCATATTGGCCCATCCTGAAAATCCTGGGTTGATTGGTCCTAATGATCACTACAGACATAGGACTGCAGTTCCCCCGCCCTTTCAAGTTCCACAGCCCCATACAAGATGGCCAAGTGACCTCAACTCCTACACGGGCAGTTTTGCTCATTCCCGTCCTCCAAGGACGGAGCTGGCCAGTGTTGGTCGGCGATGTCGTCCATTTTCTGGTGGTGCTCCATTTGTCACATGCAACAATTGCTTTGAAATTCTGCAGCTGCCCAAAAAAGTATTGCTTATGGAAAAGAGTCAACAGAAAATACGATGTGGCGCTTGTTCTACTGTAGTTGATTTTGCAGTTTCTAATAGGAAAATTGTTCTTTCTCATCATGCTGAAATGAAACAAAATCATTCAGAAGTCAATAATGGATTGAACGAGGTGGTTAGAGACGGTTCTTCACATTCTCATGGTCATGTTTCCCGGATCTATGCTCATTTCTCTTCTGAAGATTATGATAATTCTGGTTATGATTTTCAGTCTATAGATAGAGAACCTGCTTTACCATTTCCAGAACCTAGTTCAACAGCAAGCAAGCCTCATGAGATGCAAAGCTTTCATTCTTCGTCTCCCAGTACCTCTGAGGATGACTGCAACCCACCAGAAGTTCCAATTGCTTCAAGAGATGTCACAAATTCTGTTCAGCAGCCTATTAAAGCCACTTTTTCGCCACCACCTCCTGGTTCACCTCTGCAGGATCATTTTGATTACTCAGGTAATAATGTGGTAAACCGCCTTGGGAAGGGAAACCGAAGTAGTCGCTCAGATCAAGAGAAGGTGAAGCCAAATAAGATTACATCGAGACAAAATTCCTTAAAAGAGACATCATTGGCTACTGAAATGGAGGTATCATTCAATGAGTACTCTAATACTGGGGTTTCCCAAGATTCGTGGGATGCTAGCAAAGAGGAGGATCCGCCTAAAGTCAACAAAGGGGGTGATTCATTCATTGCAAACTTTATTAAGAAGAGCTTTAGAGATCTTTCCAAGTCGAATCAGACAAACGAGCATGGTAGGAGCAGTGTTTCAATTAATGGGCACCCCATACCAGACCGTGTGGTGAAGAAGGCTGAAAAGATTGCTGGAACAGTCCATCCTGGACAATATTGGTATGATTTCCGAGCAGGATTCTGGGGTATCATGGGTGGGCCTGGTCAGGGCATAATTCCTCCATTTATTGAAGAGTTCAATTACCCGATGCCTGAGAACTGTGCTGGTGGAAATACTGGTATTTTTGTAAATGGAAGAGAGCTTCATGAAAGGGATTTGGATTTGTTGGCCAGTAGAGGACTTCCCACTGCCAGAGATAGATCTTACATCATTGAGATCTCAGGGAGAGTCCTAGATGAGGACACTGGTGAAGAACTTGATAGCCTTGGCAAGCTTGCCCCAACGGTTCAGAAGGTGAAACGAGGATTTGGCATGAAGCCTCCAAAAGTACCTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like

Protein Analysis

922

Amino Acids

101.62

Weight (kDa)

6.35

Isoelectric Point (pI)

47.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EDR4-like_1st	PF22910	7 - 40	1.1e-20	Enhanced disease resistance 4-like, N-terminal domain
Zn_ribbon_12	PF11331	498 - 541	1.3e-21	EDR4 zinc-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003061)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g12570 FvH4_3g12570 FvH4_6g07650 FvH4_7g19271 FvH4_7g19272
malus_domestica	MD07G1173200.v1.1
prunus_persica	Prupe.2G212400_v2.0.a1 Prupe.5G001500_v2.0.a1
pyrus_communis	pycom07g16790
rosa_chinensis	RchiOBHm_Chr1g0363121 RchiOBHm_Chr1g0363131 RchiOBHm_Chr1g0363141
rosa_laevigata	RLG00000027589 RLG00000027591 RLG00000027592
rosa_multiflora	Rmu_co8316487.1_g000001 Rmu_sc0004472.1_g000010 Rmu_sc0005586.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293420 Rroxscaffold_4G00293430 Rroxscaffold_4G00293450
rosa_rugosa	Rorug01G0304700 Rorug01G0304700 Rorug01G0304800 Rorug01G0304900 Rorug01G0305000
rosa_samantha	Rh1AG313600 Rh1AG313700 Rh1BG277000 Rh1BG277100 Rh1BG277200 Rh1CG293600 Rh1CG293700 Rh1DG307600 Rh1DG307700 Rh1DG307800
rosa_wichuraiana	Rw1G027790 Rw1G027800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1825
AccB1I	GGYRCC	2 cut(s)	36, 2358
AccB7I	CCANNNNNTGG	2 cut(s)	1490, 2000
AccI	GTMKAC	1 cut(s)	871
AciI	CCGC	4 cut(s)	1219, 1361, 2053, 2237
AclWI	GGATC	9 cut(s)	747, 812, 1025, 1038, 1133, 1754, 2025, 2228, 2241
AcoI	YGGCCR	4 cut(s)	961, 1394, 1458, 2598
AcsI	RAATTY	3 cut(s)	1530, 1954, 2128
AcuI	CTGAAG	3 cut(s)	612, 843, 1788
AfaI	GTAC	5 cut(s)	396, 887, 1897, 2183, 2760
AfiI	CCNNNNNNNGG	9 cut(s)	596, 670, 797, 827, 1490, 2000, 2062, 2477, 2668
AflII	CTTAAG	1 cut(s)	469
AjnI	CCWGG	5 cut(s)	847, 1309, 1999, 2416, 2470
AjuI	GAANNNNNNNTTGG	2 cut(s)	859, 891
AloI	GAACNNNNNNTCC	2 cut(s)	2398, 2430
Alw21I	GWGCWC	2 cut(s)	1063, 1501
Alw26I	GTCTC	4 cut(s)	1704, 1893, 2116, 2135
AlwI	GGATC	9 cut(s)	747, 812, 1025, 1038, 1133, 1754, 2025, 2228, 2241
AoxI	GGCC	7 cut(s)	367, 961, 1293, 1394, 1458, 2468, 2598
ApeKI	GCWGC	7 cut(s)	338, 407, 494, 712, 1537, 1540, 1966
ApoI	RAATTY	3 cut(s)	1530, 1954, 2128
AseI	ATTAAT	1 cut(s)	2352
AspA2I	CCTAGG	1 cut(s)	1076
AspLEI	GCGC	1 cut(s)	1595
AspS9I	GGNCC	7 cut(s)	599, 863, 893, 1040, 1294, 1322, 2468
AsuC2I	CCSGG	2 cut(s)	399, 1744
AsuHPI	GGTGA	7 cut(s)	1166, 1998, 2110, 2270, 2392, 2690, 2737
AsuII	TTCGAA	1 cut(s)	535
AsuNHI	GCTAGC	2 cut(s)	67, 2219
AvaII	GGWCC	5 cut(s)	599, 863, 893, 1040, 1322
AvrII	CCTAGG	1 cut(s)	1076
BaeGI	GKGCMC	2 cut(s)	41, 2361
BaeI	ACNNNNGTAYC	2 cut(s)	237, 270
BalI	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
BamHI	GGATCC	2 cut(s)	1030, 2233
BanI	GGYRCC	2 cut(s)	36, 2358
BanII	GRGCYC	1 cut(s)	1063
Bbv12I	GWGCWC	2 cut(s)	1063, 1501
BbvCI	CCTCAGC	2 cut(s)	507, 716
BbvI	GCAGC	7 cut(s)	325, 419, 506, 699, 1527, 1549, 1978
BccI	CCATC	9 cut(s)	131, 484, 610, 662, 791, 1102, 1305, 1386, 2421
BciT130I	CCWGG	5 cut(s)	849, 1311, 2001, 2418, 2472
BciVI	GTATCC	1 cut(s)	136
BclI	TGATCA	2 cut(s)	283, 1330
BcnI	CCSGG	2 cut(s)	399, 1744
BcoDI	GTCTC	4 cut(s)	1704, 1893, 2116, 2135
BfaI	CTAG	7 cut(s)	68, 465, 981, 1077, 1839, 2220, 2663
BfmI	CTRYAG	7 cut(s)	357, 1336, 1350, 1535, 1605, 1803, 2012
BfoI	RGCGCY	1 cut(s)	1596
BfrI	CTTAAG	1 cut(s)	469
BfuAI	ACCTGC	1 cut(s)	1825
BfuI	GTATCC	1 cut(s)	136
BglII	AGATCT	3 cut(s)	2299, 2630, 2646
BisI	GCNGC	7 cut(s)	339, 408, 495, 713, 1538, 1541, 1967
BlnI	CCTAGG	1 cut(s)	1076
BlpI	GCTNAGC	1 cut(s)	708
BlsI	GCNGC	7 cut(s)	340, 409, 496, 714, 1539, 1542, 1968
BmcAI	AGTACT	2 cut(s)	887, 2183
Bme1390I	CCNGG	7 cut(s)	399, 849, 1311, 1744, 2001, 2418, 2472
Bme18I	GGWCC	5 cut(s)	599, 863, 893, 1040, 1322
BmgT120I	GGNCC	7 cut(s)	599, 863, 893, 1040, 1294, 1322, 2468
BmiI	GGNNCC	9 cut(s)	38, 460, 600, 789, 810, 1032, 1042, 2235, 2360
BmrFI	CCNGG	7 cut(s)	399, 849, 1311, 1744, 2001, 2418, 2472
BmrI	ACTGGG	3 cut(s)	361, 1887, 2202
BmsI	GCATC	4 cut(s)	298, 1857, 2206, 2508
BmtI	GCTAGC	2 cut(s)	71, 2223
BmuI	ACTGGG	3 cut(s)	361, 1887, 2202
BpmI	CTGGAG	1 cut(s)	798
Bpu10I	CCTNAGC	4 cut(s)	63, 507, 716, 939
Bpu1102I	GCTNAGC	1 cut(s)	708
Bpu14I	TTCGAA	1 cut(s)	535
BpuMI	CCSGG	2 cut(s)	399, 1744
BsaBI	GATNNNNATC	1 cut(s)	903
BsaJI	CCNNGG	6 cut(s)	398, 1076, 1310, 1445, 2056, 2695
BsaXI	ACNNNNNCTCC	2 cut(s)	1394, 1424
Bsc4I	CCNNNNNNNGG	9 cut(s)	596, 670, 797, 827, 1490, 2000, 2062, 2477, 2668
Bse1I	ACTGG	9 cut(s)	356, 613, 1232, 1461, 1893, 2197, 2551, 2601, 2680
Bse3DI	GCAATG	3 cut(s)	426, 1173, 2268
Bse8I	GATNNNNATC	1 cut(s)	903
BseBI	CCWGG	5 cut(s)	849, 1311, 2001, 2418, 2472
BseDI	CCNNGG	6 cut(s)	398, 1076, 1310, 1445, 2056, 2695
BseJI	GATNNNNATC	1 cut(s)	903
BseLI	CCNNNNNNNGG	9 cut(s)	596, 670, 797, 827, 1490, 2000, 2062, 2477, 2668
BseMI	GCAATG	3 cut(s)	426, 1173, 2268
BseNI	ACTGG	9 cut(s)	356, 613, 1232, 1461, 1893, 2197, 2551, 2601, 2680
BseRI	GAGGAG	4 cut(s)	195, 524, 780, 2243
BseSI	GKGCMC	2 cut(s)	41, 2361
BseXI	GCAGC	7 cut(s)	325, 419, 506, 699, 1527, 1549, 1978
BshFI	GGCC	7 cut(s)	369, 963, 1295, 1396, 1460, 2470, 2600
BshNI	GGYRCC	2 cut(s)	36, 2358
BsiHKAI	GWGCWC	2 cut(s)	1063, 1501
BsiSI	CCGG	2 cut(s)	398, 1744
BslFI	GGGAC	1 cut(s)	673
BslI	CCNNNNNNNGG	9 cut(s)	596, 670, 797, 827, 1490, 2000, 2062, 2477, 2668
BsmAI	GTCTC	4 cut(s)	1704, 1893, 2116, 2135
BsmBI	CGTCTC	2 cut(s)	1704, 1893
BsmFI	GGGAC	1 cut(s)	673
BsmI	GAATGC	1 cut(s)	409
BsnI	GGCC	7 cut(s)	369, 963, 1295, 1396, 1460, 2470, 2600
Bsp119I	TTCGAA	1 cut(s)	535
Bsp1286I	GDGCHC	4 cut(s)	41, 1063, 1501, 2361
Bsp1720I	GCTNAGC	1 cut(s)	708
BspACI	CCGC	4 cut(s)	1219, 1361, 2053, 2237
BspANI	GGCC	7 cut(s)	369, 963, 1295, 1396, 1460, 2470, 2600
BspHI	TCATGA	2 cut(s)	1861, 2575
BspLI	GGNNCC	9 cut(s)	38, 460, 600, 789, 810, 1032, 1042, 2235, 2360
BspMAI	CTGCAG	3 cut(s)	1354, 1539, 2016
BspMI	ACCTGC	1 cut(s)	1825
BspOI	GCTAGC	2 cut(s)	71, 2223
BspPI	GGATC	9 cut(s)	747, 812, 1025, 1038, 1133, 1754, 2025, 2228, 2241
BspQI	GCTCTTC	1 cut(s)	2282
BspT104I	TTCGAA	1 cut(s)	535
BspT107I	GGYRCC	2 cut(s)	36, 2358
BspTI	CTTAAG	1 cut(s)	469
BsrDI	GCAATG	3 cut(s)	426, 1173, 2268
BsrI	ACTGG	9 cut(s)	356, 613, 1232, 1461, 1893, 2197, 2551, 2601, 2680
BssECI	CCNNGG	6 cut(s)	398, 1076, 1310, 1445, 2056, 2695
BssT1I	CCWWGG	4 cut(s)	1076, 1445, 2056, 2695
Bst2UI	CCWGG	5 cut(s)	849, 1311, 2001, 2418, 2472
Bst4CI	ACNGT	9 cut(s)	175, 1013, 1119, 1606, 1715, 2375, 2410, 2531, 2716
Bst6I	CTCTTC	6 cut(s)	421, 588, 1768, 2282, 2496, 2560
BstAFI	CTTAAG	1 cut(s)	469
BstAPI	GCANNNNNTGC	2 cut(s)	416, 1521
BstBI	TTCGAA	1 cut(s)	535
BstC8I	GCNNGC	8 cut(s)	69, 504, 1458, 1852, 1856, 2221, 2702, 2706
BstENI	CCTNNNNNAGG	2 cut(s)	2060, 2666
BstH2I	RGCGCY	1 cut(s)	1596
BstHHI	GCGC	1 cut(s)	1595
BstMAI	GTCTC	4 cut(s)	1704, 1893, 2116, 2135
BstMWI	GCNNNNNNNGC	6 cut(s)	197, 416, 491, 503, 721, 1521
BstNI	CCWGG	5 cut(s)	849, 1311, 2001, 2418, 2472
BstNSI	RCATGY	1 cut(s)	1515
BstSCI	CCNGG	7 cut(s)	397, 847, 1309, 1742, 1999, 2416, 2470
BstSFI	CTRYAG	7 cut(s)	357, 1336, 1350, 1535, 1605, 1803, 2012
BstSLI	GKGCMC	2 cut(s)	41, 2361
BstV1I	GCAGC	7 cut(s)	325, 419, 506, 699, 1527, 1549, 1978
BstX2I	RGATCY	7 cut(s)	817, 1030, 1746, 2233, 2299, 2630, 2646
BstYI	RGATCY	7 cut(s)	817, 1030, 1746, 2233, 2299, 2630, 2646
BsuI	GTATCC	1 cut(s)	136
BsuRI	GGCC	7 cut(s)	369, 963, 1295, 1396, 1460, 2470, 2600
BtgZI	GCGATG	1 cut(s)	1489
BtsI	GCAGTG	3 cut(s)	1242, 2347, 2616
BtsIMutI	CAGTG	7 cut(s)	95, 1060, 1242, 1468, 2347, 2616, 2673
BveI	ACCTGC	1 cut(s)	1825
Cac8I	GCNNGC	8 cut(s)	69, 504, 1458, 1852, 1856, 2221, 2702, 2706
CciI	TCATGA	2 cut(s)	1861, 2575
CfoI	GCGC	1 cut(s)	1595
Cfr13I	GGNCC	7 cut(s)	599, 863, 893, 1040, 1294, 1322, 2468
Csp6I	GTAC	5 cut(s)	395, 886, 1896, 2182, 2759
CspCI	CAANNNNNGTGG	2 cut(s)	2192, 2227
CviQI	GTAC	5 cut(s)	395, 886, 1896, 2182, 2759
EaeI	YGGCCR	4 cut(s)	961, 1394, 1458, 2598
Eam1104I	CTCTTC	6 cut(s)	421, 588, 1768, 2282, 2496, 2560
EarI	CTCTTC	6 cut(s)	421, 588, 1768, 2282, 2496, 2560
EciI	GGCGGA	1 cut(s)	2226
Ecl136II	GAGCTC	1 cut(s)	1061
Eco130I	CCWWGG	4 cut(s)	1076, 1445, 2056, 2695
Eco24I	GRGCYC	1 cut(s)	1063
Eco47I	GGWCC	5 cut(s)	599, 863, 893, 1040, 1322
Eco53kI	GAGCTC	1 cut(s)	1061
Eco57I	CTGAAG	3 cut(s)	612, 843, 1788
EcoICRI	GAGCTC	1 cut(s)	1061
EcoNI	CCTNNNNNAGG	2 cut(s)	2060, 2666
EcoRII	CCWGG	5 cut(s)	847, 1309, 1999, 2416, 2470
EcoT14I	CCWWGG	4 cut(s)	1076, 1445, 2056, 2695
EcoT22I	ATGCAT	1 cut(s)	1002
EcoT38I	GRGCYC	1 cut(s)	1063
ErhI	CCWWGG	4 cut(s)	1076, 1445, 2056, 2695
Esp3I	CGTCTC	2 cut(s)	1704, 1893
FalI	AAGNNNNNCTT	6 cut(s)	859, 891, 1542, 1574, 2276, 2308
FaqI	GGGAC	1 cut(s)	673
FauI	CCCGC	1 cut(s)	1368
FauNDI	CATATG	1 cut(s)	1144
FbaI	TGATCA	2 cut(s)	283, 1330
FblI	GTMKAC	1 cut(s)	871
Fnu4HI	GCNGC	7 cut(s)	339, 408, 495, 713, 1538, 1541, 1967
FriOI	GRGCYC	1 cut(s)	1063
Fsp4HI	GCNGC	7 cut(s)	339, 408, 495, 713, 1538, 1541, 1967
FspBI	CTAG	7 cut(s)	68, 465, 981, 1077, 1839, 2220, 2663
GlaI	GCGC	1 cut(s)	1594
GluI	GCNGC	7 cut(s)	339, 408, 495, 713, 1538, 1541, 1967
GsuI	CTGGAG	1 cut(s)	798
HaeII	RGCGCY	1 cut(s)	1596
HaeIII	GGCC	7 cut(s)	369, 963, 1295, 1396, 1460, 2470, 2600
HapII	CCGG	2 cut(s)	398, 1744
HhaI	GCGC	1 cut(s)	1595
Hin6I	GCGC	1 cut(s)	1593
HinP1I	GCGC	1 cut(s)	1593
HincII	GTYRAC	2 cut(s)	1574, 2248
HindII	GTYRAC	2 cut(s)	1574, 2248
HindIII	AAGCTT	3 cut(s)	1261, 1873, 2702
HinfI	GANTC	9 cut(s)	8, 52, 452, 1570, 2207, 2261, 2314, 2448, 2658
HpaII	CCGG	2 cut(s)	398, 1744
HphI	GGTGA	7 cut(s)	1166, 1998, 2110, 2270, 2392, 2690, 2737
Hpy166II	GTNNAC	5 cut(s)	872, 1574, 2006, 2050, 2248
Hpy8I	GTNNAC	5 cut(s)	872, 1574, 2006, 2050, 2248
HpyCH4III	ACNGT	9 cut(s)	175, 1013, 1119, 1606, 1715, 2375, 2410, 2531, 2716
HpyF10VI	GCNNNNNNNGC	6 cut(s)	197, 416, 491, 503, 721, 1521
HspAI	GCGC	1 cut(s)	1593
Ksp22I	TGATCA	2 cut(s)	283, 1330
LguI	GCTCTTC	1 cut(s)	2282
LmnI	GCTCC	6 cut(s)	793, 1058, 1066, 1453, 1504, 2337
Lsp1109I	GCAGC	7 cut(s)	325, 419, 506, 699, 1527, 1549, 1978
LweI	GCATC	4 cut(s)	298, 1857, 2206, 2508
MaeI	CTAG	7 cut(s)	68, 465, 981, 1077, 1839, 2220, 2663
MaeIII	GTNAC	4 cut(s)	1193, 1400, 1507, 1948
MfeI	CAATTG	2 cut(s)	1519, 1932
MflI	RGATCY	7 cut(s)	817, 1030, 1746, 2233, 2299, 2630, 2646
MhlI	GDGCHC	4 cut(s)	41, 1063, 1501, 2361
MlsI	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
MluNI	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
MlyI	GAGTC	2 cut(s)	1579, 2667
MmeI	TCCRAC	1 cut(s)	141
Mox20I	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
Mph1103I	ATGCAT	1 cut(s)	1002
MscI	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
MseI	TTAA	8 cut(s)	230, 255, 470, 927, 1974, 2135, 2283, 2352
MslI	CAYNNNNRTG	2 cut(s)	1728, 1734
Msp20I	TGGCCA	4 cut(s)	963, 1396, 1460, 2600
MspA1I	CMGCKG	1 cut(s)	1540
MspCI	CTTAAG	1 cut(s)	469
MspI	CCGG	2 cut(s)	398, 1744
MspR9I	CCNGG	7 cut(s)	399, 849, 1311, 1744, 2001, 2418, 2472
MunI	CAATTG	2 cut(s)	1519, 1932
Mva1269I	GAATGC	1 cut(s)	409
MvaI	CCWGG	5 cut(s)	849, 1311, 2001, 2418, 2472
MwoI	GCNNNNNNNGC	6 cut(s)	197, 416, 491, 503, 721, 1521
NciI	CCSGG	2 cut(s)	399, 1744
NdeI	CATATG	1 cut(s)	1144
NheI	GCTAGC	2 cut(s)	67, 2219
NlaIV	GGNNCC	9 cut(s)	38, 460, 600, 789, 810, 1032, 1042, 2235, 2360
NmuCI	GTSAC	3 cut(s)	1400, 1507, 1948
NsiI	ATGCAT	1 cut(s)	1002
NspI	RCATGY	1 cut(s)	1515
NspV	TTCGAA	1 cut(s)	535
PagI	TCATGA	2 cut(s)	1861, 2575
PciSI	GCTCTTC	1 cut(s)	2282
PcsI	WCGNNNNNNNCGW	1 cut(s)	1477
PctI	GAATGC	1 cut(s)	409
PfeI	GAWTC	7 cut(s)	8, 52, 452, 2207, 2261, 2314, 2448
PflMI	CCANNNNNTGG	2 cut(s)	1490, 2000
PfoI	TCCNGGA	2 cut(s)	1742, 2416
PkrI	GCNGC	7 cut(s)	340, 409, 496, 714, 1539, 1542, 1968
PleI	GAGTC	2 cut(s)	1578, 2666
PpsI	GAGTC	2 cut(s)	1578, 2666
PshBI	ATTAAT	1 cut(s)	2352
Psp124BI	GAGCTC	1 cut(s)	1063
Psp6I	CCWGG	5 cut(s)	847, 1309, 1999, 2416, 2470
PspGI	CCWGG	5 cut(s)	847, 1309, 1999, 2416, 2470
PspN4I	GGNNCC	9 cut(s)	38, 460, 600, 789, 810, 1032, 1042, 2235, 2360
PspPI	GGNCC	7 cut(s)	599, 863, 893, 1040, 1294, 1322, 2468
PsrI	GAACNNNNNNTAC	2 cut(s)	1806, 1838
PstI	CTGCAG	3 cut(s)	1354, 1539, 2016
PsuI	RGATCY	7 cut(s)	817, 1030, 1746, 2233, 2299, 2630, 2646
PvuII	CAGCTG	1 cut(s)	1540
RsaI	GTAC	5 cut(s)	396, 887, 1897, 2183, 2760
RsaNI	GTAC	5 cut(s)	395, 886, 1896, 2182, 2759
RseI	CAYNNNNRTG	2 cut(s)	1728, 1734
SacI	GAGCTC	1 cut(s)	1063
SapI	GCTCTTC	1 cut(s)	2282
SaqAI	TTAA	8 cut(s)	230, 255, 470, 927, 1974, 2135, 2283, 2352
SatI	GCNGC	7 cut(s)	339, 408, 495, 713, 1538, 1541, 1967
Sau96I	GGNCC	7 cut(s)	599, 863, 893, 1040, 1294, 1322, 2468
ScaI	AGTACT	2 cut(s)	887, 2183
SchI	GAGTC	2 cut(s)	1579, 2667
ScrFI	CCNGG	7 cut(s)	399, 849, 1311, 1744, 2001, 2418, 2472
SduI	GDGCHC	4 cut(s)	41, 1063, 1501, 2361
SfaNI	GCATC	4 cut(s)	298, 1857, 2206, 2508
SfcI	CTRYAG	7 cut(s)	357, 1336, 1350, 1535, 1605, 1803, 2012
SfuI	TTCGAA	1 cut(s)	535
SinI	GGWCC	5 cut(s)	599, 863, 893, 1040, 1322
SmiMI	CAYNNNNRTG	2 cut(s)	1728, 1734
SmlI	CTYRAG	1 cut(s)	469
SmoI	CTYRAG	1 cut(s)	469
SsiI	CCGC	4 cut(s)	1219, 1361, 2053, 2237
SspI	AATATT	1 cut(s)	2426
SspMI	CTAG	7 cut(s)	68, 465, 981, 1077, 1839, 2220, 2663
SstI	GAGCTC	1 cut(s)	1063
StyD4I	CCNGG	7 cut(s)	397, 847, 1309, 1742, 1999, 2416, 2470
StyI	CCWWGG	4 cut(s)	1076, 1445, 2056, 2695
TaaI	ACNGT	9 cut(s)	175, 1013, 1119, 1606, 1715, 2375, 2410, 2531, 2716
TaqI	TCGA	4 cut(s)	535, 776, 2120, 2312
TaqII	GACCGA	2 cut(s)	717, 741
TatI	WGTACW	2 cut(s)	885, 2181
TfiI	GAWTC	7 cut(s)	8, 52, 452, 2207, 2261, 2314, 2448
Tru1I	TTAA	8 cut(s)	230, 255, 470, 927, 1974, 2135, 2283, 2352
Tru9I	TTAA	8 cut(s)	230, 255, 470, 927, 1974, 2135, 2283, 2352
TscAI	CASTG	7 cut(s)	95, 1060, 1249, 1468, 2347, 2623, 2680
TseFI	GTSAC	3 cut(s)	1400, 1507, 1948
TseI	GCWGC	7 cut(s)	338, 407, 494, 712, 1537, 1540, 1966
Tsp45I	GTSAC	3 cut(s)	1400, 1507, 1948
TspGWI	ACGGA	2 cut(s)	1053, 1466
TspRI	CASTG	7 cut(s)	95, 1060, 1249, 1468, 2347, 2623, 2680
Van91I	CCANNNNNTGG	2 cut(s)	1490, 2000
Vha464I	CTTAAG	1 cut(s)	469
VpaK11BI	GGWCC	5 cut(s)	599, 863, 893, 1040, 1322
VspI	ATTAAT	1 cut(s)	2352
XagI	CCTNNNNNAGG	2 cut(s)	2060, 2666
XapI	RAATTY	3 cut(s)	1530, 1954, 2128
XceI	RCATGY	1 cut(s)	1515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1052
XmaJI	CCTAGG	1 cut(s)	1076
XmiI	GTMKAC	1 cut(s)	871
XspI	CTAG	7 cut(s)	68, 465, 981, 1077, 1839, 2220, 2663
ZrmI	AGTACT	2 cut(s)	887, 2183
Zsp2I	ATGCAT	1 cut(s)	1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.