Rroxscaffold_4G00301310

Acyclic terpene utilisation family protein AtuA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002235
Physical Location & Seq
Reverse (-)
21699865 .. 21721649
21785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301310.1

Sequence Viewer

Length: 516 bp
ATGTTTGGAAAAGTATCAGCCAAATGTAATTATAACTTCACGGGTTGCAGATGCTGCCTTATTTTTAGCACCAACGGGGACAAGTCTCGAGACATGTCTTCCTCTCAGCTCCTAGACTTGTCACTTCCTTATGCTGAAATTAGCTCTGATGGAAAAGTATGTGTAGCGAAGGCAGAGGGTAGTGGTGGCCTTTTGAACTTCAGTACATGTGCTCAACAACTTCTTTATGAGATCGGGAAACTGGGTACTTATGCAACTCCTGATGTGATAATTGATATCCGGGATGTTTCATTCTACCCATTATCAAGTTGTAAGGTCCTCTGTGCTGGAGCAAAACCATCTGCAGTATCAGTTCCTGATAAACTCCTGCGGTTGGTTCCAAAGGACTATGGTTGGAAAGGATGGGGTGAAATATCTTATGGAGGATATGAATGTGTTAAACGTGCCAAGATTGCTGAATATCGGAAAGTGGAAGGAGGTTTCGTAAGTTTGGGTTATGTGCCATATTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AtuA

Protein Analysis

171

Amino Acids

18.58

Weight (kDa)

8.52

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtuA PF07287 26 - 153 1e-26 Acyclic terpene utilisation family protein AtuA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 33
AciI CCGC 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 184
AfaI GTAC 2 cut(s) 205, 247
AfiI CCNNNNNNNGG 1 cut(s) 373
AflIII ACRYGT 2 cut(s) 93, 206
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 2 cut(s) 109, 144
AluI AGCT 2 cut(s) 109, 144
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 2 cut(s) 84, 90
AlwNI CAGNNNCTG 2 cut(s) 54, 356
Ama87I CYCGRG 1 cut(s) 87
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 87
AvaII GGWCC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 41
BccI CCATC 3 cut(s) 143, 346, 396
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 84, 90
BfaI CTAG 1 cut(s) 113
BfmI CTRYAG 1 cut(s) 342
BisI GCNGC 1 cut(s) 55
BlsI GCNGC 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 1 cut(s) 281
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 41
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 1 cut(s) 246
BseGI GGATG 2 cut(s) 289, 407
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 246
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 280
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 84, 90
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 378
BspMAI CTGCAG 1 cut(s) 346
BsrI ACTGG 1 cut(s) 246
BssMI GATC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 105
BstF5I GGATG 2 cut(s) 289, 407
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 2 cut(s) 84, 90
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 54, 452
BstNSI RCATGY 2 cut(s) 97, 210
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 90
BsuRI GGCC 1 cut(s) 189
BtsCI GGATG 2 cut(s) 289, 407
CaiI CAGNNNCTG 2 cut(s) 54, 356
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 204, 246
CviAII CATG 2 cut(s) 94, 207
CviJI RGCY 4 cut(s) 20, 109, 144, 189
CviKI_1 RGCY 4 cut(s) 20, 109, 144, 189
CviQI GTAC 2 cut(s) 204, 246
DdeI CTNAG 1 cut(s) 105
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 316
EcoRV GATATC 1 cut(s) 277
FaeI CATG 2 cut(s) 97, 210
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 93, 206
Fnu4HI GCNGC 1 cut(s) 55
FokI GGATG 2 cut(s) 296, 414
Fsp4HI GCNGC 1 cut(s) 55
FspBI CTAG 1 cut(s) 113
GluI GCNGC 1 cut(s) 55
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 189
HapII CCGG 1 cut(s) 280
Hin1II CATG 2 cut(s) 97, 210
HpaII CCGG 1 cut(s) 280
HphI GGTGA 1 cut(s) 419
Hpy188I TCNGA 2 cut(s) 148, 465
Hpy188III TCNNGA 5 cut(s) 87, 89, 235, 260, 356
HpyAV CCTTC 2 cut(s) 163, 467
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 3 cut(s) 48, 254, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 452
HpyF3I CTNAG 1 cut(s) 105
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 2 cut(s) 97, 210
Kzo9I GATC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 114, 329
LpnPI CCDG 6 cut(s) 227, 273, 293, 312, 369, 380
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 41
MaeI CTAG 1 cut(s) 113
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 120
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 28, 138, 270
MmeI TCCRAC 1 cut(s) 374
MnlI CCTC 5 cut(s) 112, 169, 329, 416, 470
MseI TTAA 1 cut(s) 438
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 54, 452
NciI CCSGG 1 cut(s) 281
NdeII GATC 1 cut(s) 231
NlaIII CATG 2 cut(s) 97, 210
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 120
NspI RCATGY 2 cut(s) 97, 210
PaeR7I CTCGAG 1 cut(s) 87
PciI ACATGT 2 cut(s) 93, 206
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 1 cut(s) 56
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 2 cut(s) 93, 206
PsiI TTATAA 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 2 cut(s) 54, 356
RsaI GTAC 2 cut(s) 205, 247
RsaNI GTAC 2 cut(s) 204, 246
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 1 cut(s) 55
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 214
SetI ASST 5 cut(s) 111, 146, 318, 445, 481
SfaNI GCATC 1 cut(s) 41
SfcI CTRYAG 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 87
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 3 cut(s) 28, 138, 270
SsiI CCGC 1 cut(s) 370
SspMI CTAG 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 279
TaiI ACGT 1 cut(s) 445
TaqI TCGA 1 cut(s) 88
TasI AATT 3 cut(s) 28, 138, 270
TatI WGTACW 1 cut(s) 203
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 279, 444
VpaK11BI GGWCC 1 cut(s) 316
XceI RCATGY 2 cut(s) 97, 210
XhoI CTCGAG 1 cut(s) 87
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.