Rorug01G0240300

Acyclic terpene utilisation family protein AtuA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002235
Physical Location & Seq
Forward (+)
34567870 .. 34568831
962 bp
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UTR
Exon/CDS
Intron
Rorug01G0240300.1

Sequence Viewer

Length: 306 bp
ATGACTTCGTTACGAAGATCATCCATGTCCTTCAGGAGGCAAGGCTCTTCCGGCCGCATTTGGGAAGATAGGCTTCAAATAGTAGAACATAAGTCTGGCCACGCTCAGGCCATCTTTTCCGGTGACAAAAGCAACGACGAGGATCTCCGAAATCTTGATGGAAGATTCCAAGACAGACGGCATGTTGATTTAGGTTCTCCATCGAGTCCGTCTACGCCTAATTCGAAGCAAGAACAAAAGGCAGCTCAAAGGTGTGCTCTTTCTTCCATTTTTGGGCGATGTATGGGGTCTCCTACGGCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AtuA

Protein Analysis

101

Amino Acids

11.21

Weight (kDa)

10.02

Isoelectric Point (pI)

83.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 52 1.7e-08 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 212
AciI CCGC 1 cut(s) 55
AclWI GGATC 1 cut(s) 150
AcoI YGGCCR 2 cut(s) 52, 97
AcuI CTGAAG 1 cut(s) 16
AfiI CCNNNNNNNGG 4 cut(s) 36, 61, 106, 273
AgsI TTSAA 1 cut(s) 77
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 150
AoxI GGCC 3 cut(s) 52, 97, 108
ApeKI GCWGC 2 cut(s) 242, 299
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 224
BalI TGGCCA 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 254
BccI CCATC 3 cut(s) 119, 152, 208
BceAI ACGGC 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 1 cut(s) 304
BisI GCNGC 3 cut(s) 55, 243, 300
BlsI GCNGC 3 cut(s) 56, 244, 301
Bpu10I CCTNAGC 1 cut(s) 105
Bpu14I TTCGAA 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 61, 106, 273
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 4 cut(s) 36, 61, 106, 273
BseMII CTCAG 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 54, 99, 110
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 2 cut(s) 51, 120
BslI CCNNNNNNNGG 4 cut(s) 36, 61, 106, 273
BsmAI GTCTC 1 cut(s) 294
BsnI GGCC 3 cut(s) 54, 99, 110
Bso31I GGTCTC 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 2 cut(s) 17, 142
BspACI CCGC 1 cut(s) 55
BspANI GGCC 3 cut(s) 54, 99, 110
BspCNI CTCAG 1 cut(s) 118
BspPI GGATC 1 cut(s) 150
BspQI GCTCTTC 1 cut(s) 52
BspT104I TTCGAA 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 294
BssMI GATC 2 cut(s) 17, 142
Bst6I CTCTTC 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 105
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 20
BstKTI GATC 2 cut(s) 20, 145
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 17, 142
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNSI RCATGY 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BstZI CGGCCG 1 cut(s) 52
BsuRI GGCC 3 cut(s) 54, 99, 110
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 20
CviAII CATG 2 cut(s) 25, 182
CviJI RGCY 7 cut(s) 45, 54, 73, 99, 110, 245, 302
CviKI_1 RGCY 7 cut(s) 45, 54, 73, 99, 110, 245, 302
DdeI CTNAG 1 cut(s) 105
DpnI GATC 2 cut(s) 19, 144
DpnII GATC 2 cut(s) 17, 142
EaeI YGGCCR 2 cut(s) 52, 97
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 16
EcoNI CCTNNNNNAGG 1 cut(s) 34
FaeI CATG 2 cut(s) 28, 185
FaiI YATR 4 cut(s) 26, 90, 183, 284
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FatI CATG 2 cut(s) 24, 181
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 55, 243, 300
FokI GGATG 1 cut(s) 7
Fsp4HI GCNGC 3 cut(s) 55, 243, 300
FspBI CTAG 1 cut(s) 304
GluI GCNGC 3 cut(s) 55, 243, 300
HaeIII GGCC 3 cut(s) 54, 99, 110
HapII CCGG 2 cut(s) 51, 120
Hin1II CATG 2 cut(s) 28, 185
HinfI GANTC 2 cut(s) 165, 205
HpaII CCGG 2 cut(s) 51, 120
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 1 cut(s) 149
Hpy188III TCNNGA 2 cut(s) 34, 155
Hpy8I GTNNAC 1 cut(s) 213
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 2 cut(s) 28, 185
Kzo9I GATC 2 cut(s) 17, 142
LguI GCTCTTC 1 cut(s) 52
LpnPI CCDG 5 cut(s) 19, 64, 81, 92, 133
Lsp1109I GCAGC 1 cut(s) 254
MaeI CTAG 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 9, 122
MalI GATC 2 cut(s) 19, 144
MboI GATC 2 cut(s) 17, 142
MboII GAAGA 5 cut(s) 27, 39, 77, 174, 255
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 1 cut(s) 220
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 1 cut(s) 214
MnlI CCTC 2 cut(s) 30, 133
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 99
MspI CCGG 2 cut(s) 51, 120
MwoI GCNNNNNNNGC 1 cut(s) 51
NdeII GATC 2 cut(s) 17, 142
NlaIII CATG 2 cut(s) 28, 185
NmuCI GTSAC 1 cut(s) 122
NspI RCATGY 1 cut(s) 185
NspV TTCGAA 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PfeI GAWTC 1 cut(s) 165
PkrI GCNGC 3 cut(s) 56, 244, 301
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PsuI RGATCY 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 52
SatI GCNGC 3 cut(s) 55, 243, 300
Sau3AI GATC 2 cut(s) 17, 142
SchI GAGTC 1 cut(s) 214
SduI GDGCHC 1 cut(s) 259
SetI ASST 3 cut(s) 196, 247, 254
SfuI TTCGAA 1 cut(s) 224
Sse9I AATT 1 cut(s) 220
SsiI CCGC 1 cut(s) 55
SspMI CTAG 1 cut(s) 304
TaqI TCGA 2 cut(s) 203, 224
TasI AATT 1 cut(s) 220
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 1 cut(s) 165
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 2 cut(s) 242, 299
Tsp45I GTSAC 1 cut(s) 122
TspGWI ACGGA 1 cut(s) 198
XagI CCTNNNNNAGG 1 cut(s) 34
XceI RCATGY 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 212
XspI CTAG 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.