Rroxscaffold_7G00194680

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003159
Physical Location & Seq
Forward (+)
36680598 .. 36685019
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00194680.1

Sequence Viewer

Length: 606 bp
ATGGGGTTTGGTCAATTGAGAGGAGCCGAATCCACATGGCAGTGGGTTGACTCAAATCGGACCGGAAGTGTGAGAACGAACGAGCACGATCAACATGCGATCTTCTCGATGTTGGGTTGGCAGCAAGACCGTGGCTGGATTTGTAACAATCAAGTCGGTCATTGGGGGAGAAAATCGAAGGAGTCGAAGTCGGAGTTGGGGGAGGAACATGATGACCGACAAGAATTGCCTTGGCACCTGTCGTGCTGCTCAGTGCTACGTGTGTTTGCTTTACGCAACGACAGCACGGGTGAGAGGGAGATTCAGTTTACACCACATAATAAACATACCGGGTGGCTTTCTGGCAGGTATAGAGACAAAAGGATGAGGATAGACTACTTCATAGCTGCAGAGCAACTCAAAGATAGGATTGTTTCATGTGAGATGCACGGGCAAGGGATGAATTACAGGAAGGACTACTTTAAACTTCTACGTTATCAAGAGTTGCATTTTGATGATTGTGGAAATCGTGTTTCTCAGTTGTTACATGTTGAATTAGCTGCCCCAGCTTCACAGTTTCAAAACAATGATATTCTAGAAGACCTCATGATTCAAATTTGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

201

Amino Acids

23.63

Weight (kDa)

6.36

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 234
AcsI RAATTY 1 cut(s) 594
AflIII ACRYGT 2 cut(s) 259, 526
AgsI TTSAA 3 cut(s) 533, 560, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 3 cut(s) 386, 539, 548
AluI AGCT 3 cut(s) 386, 539, 548
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 121, 246, 386, 539
ApoI RAATTY 1 cut(s) 594
AspS9I GGNCC 1 cut(s) 60
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 1 cut(s) 302
AvaII GGWCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 4 cut(s) 133, 233, 373, 526
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 1 cut(s) 575
BfmI CTRYAG 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 336
BisI GCNGC 4 cut(s) 122, 247, 387, 540
BlsI GCNGC 4 cut(s) 123, 248, 388, 541
Bme1390I CCNGG 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 25, 236
BmrFI CCNGG 1 cut(s) 331
BmsI GCATC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 585
BpuMI CCSGG 1 cut(s) 331
BsaAI YACGTR 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 130, 230
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 290, 320
BseDI CCNNGG 2 cut(s) 130, 230
BseGI GGATG 2 cut(s) 369, 444
BseMII CTCAG 2 cut(s) 264, 530
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 4 cut(s) 133, 233, 373, 526
BseYI CCCAGC 1 cut(s) 544
BshNI GGYRCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 2 cut(s) 63, 330
BsmAI GTCTC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 2 cut(s) 88, 99
BspCNI CTCAG 2 cut(s) 263, 529
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 25, 236
BspMAI CTGCAG 1 cut(s) 391
BspMI ACCTGC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 234
BssECI CCNNGG 2 cut(s) 130, 230
BssMI GATC 2 cut(s) 88, 99
BssT1I CCWWGG 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 131, 555
BstBAI YACGTR 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 250, 516
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 369, 444
BstKTI GATC 2 cut(s) 91, 102
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 2 cut(s) 88, 99
BstMWI GCNNNNNNNGC 2 cut(s) 282, 545
BstNSI RCATGY 2 cut(s) 98, 530
BstSCI CCNGG 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 133, 233, 373, 526
BstV2I GAAGAC 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 2 cut(s) 369, 444
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 2 cut(s) 47, 258
BveI ACCTGC 1 cut(s) 336
CciI TCATGA 1 cut(s) 585
Cfr13I GGNCC 1 cut(s) 60
CpoI CGGWCCG 1 cut(s) 60
CspI CGGWCCG 1 cut(s) 60
CviAII CATG 6 cut(s) 36, 95, 209, 417, 527, 586
CviJI RGCY 6 cut(s) 26, 135, 337, 386, 539, 548
CviKI_1 RGCY 6 cut(s) 26, 135, 337, 386, 539, 548
DdeI CTNAG 2 cut(s) 250, 516
DpnI GATC 2 cut(s) 90, 101
DpnII GATC 2 cut(s) 88, 99
DraI TTTAAA 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 6 cut(s) 39, 98, 212, 420, 530, 589
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 6 cut(s) 35, 94, 208, 416, 526, 585
Fnu4HI GCNGC 4 cut(s) 122, 247, 387, 540
FokI GGATG 2 cut(s) 376, 451
Fsp4HI GCNGC 4 cut(s) 122, 247, 387, 540
FspBI CTAG 1 cut(s) 575
GluI GCNGC 4 cut(s) 122, 247, 387, 540
GsaI CCCAGC 1 cut(s) 548
HapII CCGG 2 cut(s) 63, 330
Hin1II CATG 6 cut(s) 39, 98, 212, 420, 530, 589
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 5 cut(s) 29, 50, 182, 301, 589
HpaII CCGG 2 cut(s) 63, 330
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 2 cut(s) 49, 309
Hpy188I TCNGA 2 cut(s) 60, 193
Hpy188III TCNNGA 4 cut(s) 106, 479, 575, 586
Hpy8I GTNNAC 2 cut(s) 49, 309
HpyAV CCTTC 2 cut(s) 172, 445
HpyCH4III ACNGT 2 cut(s) 131, 555
HpyCH4IV ACGT 2 cut(s) 259, 472
HpyCH4V TGCA 3 cut(s) 389, 427, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 545
HpyF3I CTNAG 2 cut(s) 250, 516
HpySE526I ACGT 2 cut(s) 259, 472
Hsp92II CATG 6 cut(s) 39, 98, 212, 420, 530, 589
Kzo9I GATC 2 cut(s) 88, 99
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 8 cut(s) 76, 121, 251, 327, 331, 343, 433, 558
Lsp1109I GCAGC 4 cut(s) 133, 233, 373, 526
LweI GCATC 1 cut(s) 414
MaeI CTAG 1 cut(s) 575
MaeII ACGT 2 cut(s) 259, 472
MaeIII GTNAC 2 cut(s) 143, 522
MalI GATC 2 cut(s) 90, 101
MboI GATC 2 cut(s) 88, 99
MboII GAAGA 2 cut(s) 94, 590
MfeI CAATTG 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 5 cut(s) 14, 224, 442, 533, 594
MlyI GAGTC 2 cut(s) 44, 191
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 5 cut(s) 14, 196, 288, 360, 593
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 2 cut(s) 40, 492
MspI CCGG 2 cut(s) 63, 330
MspR9I CCNGG 1 cut(s) 331
MunI CAATTG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 282, 545
NciI CCSGG 1 cut(s) 331
NdeII GATC 2 cut(s) 88, 99
NlaIII CATG 6 cut(s) 39, 98, 212, 420, 530, 589
NlaIV GGNNCC 2 cut(s) 25, 236
NspI RCATGY 2 cut(s) 98, 530
PagI TCATGA 1 cut(s) 585
PciI ACATGT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PfeI GAWTC 3 cut(s) 29, 301, 589
PkrI GCNGC 4 cut(s) 123, 248, 388, 541
PleI GAGTC 2 cut(s) 44, 190
PpsI GAGTC 2 cut(s) 44, 190
Ppu21I YACGTR 1 cut(s) 260
PscI ACATGT 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 544
PspN4I GGNNCC 2 cut(s) 25, 236
PspPI GGNCC 1 cut(s) 60
PstI CTGCAG 1 cut(s) 391
RseI CAYNNNNRTG 2 cut(s) 40, 492
Rsr2I CGGWCCG 1 cut(s) 60
RsrII CGGWCCG 1 cut(s) 60
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 4 cut(s) 122, 247, 387, 540
Sau3AI GATC 2 cut(s) 88, 99
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 2 cut(s) 44, 191
ScrFI CCNGG 1 cut(s) 331
SduI GDGCHC 1 cut(s) 87
SetI ASST 8 cut(s) 240, 262, 350, 388, 475, 541, 550, 585
SfaNI GCATC 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 40, 492
Sse9I AATT 5 cut(s) 14, 224, 442, 533, 594
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 1 cut(s) 329
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 2 cut(s) 131, 555
TaiI ACGT 2 cut(s) 262, 475
TaqI TCGA 3 cut(s) 107, 176, 185
TaqII GACCGA 2 cut(s) 146, 231
TasI AATT 5 cut(s) 14, 224, 442, 533, 594
TfiI GAWTC 3 cut(s) 29, 301, 589
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 2 cut(s) 47, 258
TseI GCWGC 4 cut(s) 121, 246, 386, 539
TspDTI ATGAA 3 cut(s) 370, 405, 455
TspRI CASTG 2 cut(s) 47, 258
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 594
XbaI TCTAGA 1 cut(s) 574
XceI RCATGY 2 cut(s) 98, 530
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.