Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0001087

Physical Location & Seq

Genomic Coordinates Forward (+)

2640728 .. 2644225

Gene Structure 3498 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0036500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGTCGAAGAAGAAAGTGAGCGGAAACACGATGACGCTCAAGGACTTTCACGGCGGTTCTATTCCCTCCGATCTCCCTCTCCCTTCTGCTCCCGGTGTAATTGTTAGGGCTACGGATCGGCCCGGTTACGACCGGCCGGCCGCTTGGGGAAACCCGATGGGGCGGCCCGATCACCGTTCCCGGCCACATACGTCTCCCGCCACCAGGCACTTTGATGACAAGACTCCGTTTCTAGCTCACTCTGTGCACATTGGGCGGAACTTTGATGAGGATGAACGCAAGCCACTGGATGGTGTGTCGGCGCCGCGCCGGATTATAAGCGATGACAGCATTCGGGTGGCCCCAGCCCGTGCCGAGCCGAAACCGGTGTTTGCATCCCCTGGGGGATTATCAGGCGTGCAGGGATGGGCTGCGGCACCGCAGTCCCCCAGGGAAGCGGTGAGTTCTTACTCAGAGAGGGTTAGTGACGCGGTGGGGGTTAATTCTCAGAGTGTAAGTGGTAATGGTGGGCGTGGCGGTGGGAGTGGTGCTCAGCCGAATGCATGGGCAGTTAGGAAGGAGATGGCGGGTGTTACTGAGCCGGTGCAATCTGCATGGTCCGGACAGAGTGCTGTTGTGAAGTTGGCTCATGCTAGTGCACTTGAGAAGGTGTCTTCGGGTAGATGGCAATCAAAGCCTTCAATCCAGTATCAGGCAAATAACATCGAGGTTGCTAGGTCAGCTGAAACAGAGAGTGGTGTACATTCCAAGGGTTATGGTAGTGACGCTTACAAGAGGATGGATGTGATGGTTGAGCGGCAAAACTATGATGCCACCTTGGCAATACAAATTGAAAGAGGTCTACAAATGGATGATGGGATTCAGAATGTTAGGAAGGAGTTGCCGGATTATGAGAGGGCTAGGGGACCCATCATTTCAGAAGTAAAAGAAAGGAGCCCAATGGTTCGGAAGGAGTTACCTGATTATGAGAGGGCTAGGGCCCCCATCGTTTCAGAAGTAAGAGAAAGGAAGCCAACTGTTTATAGTAACAGGGTTCAACCTGCTCAGGCTGATGGCAAATATGGTCAGACTGAAGTGCAGCCCTTTGCTTCTTCAGAACACGTAGAGCGCCCTAAGTTAAAGTTACTTCCAAGAACAAGGCCAGTGGACCATTTGGAAGCTCCAGTTGGTGATCATGCACAGGAGTACCAGCACCCTGTTTATGTTGAAACTGTCAATGAAGTGTATGGAAATATGAATTCTACAAAACCTGGTTCAGCAGGCTCTGAGAGTGGGAAACTGGCCGTGGAGCGTCCAAAATTGAATTTAAAGCCACGGTCTCAGCCTCTTGAACAATTGGATGCAATTGCCGAAAGAGAGAGGAATGCGTTATTTGGTGGTGCTCGCCCACGAGAACTGGTTCTTAAGGAGCGAGGGGTTGATGATGTTGTGATCAACAATGCTGACATAGTTCAGCATTCTGACAAGGTTGAGCATCATGTTGCCAGGGCTGAAAATCATGTTCCAAGGGCTGAACATCTCTCTCCAAAGCCTGATAGAGTTCCTGGGCATGTCAATCCTACTCGCCACCCTGATAAAGCTGAGAATCATCCTTTTGATCAGAGAACTGGAAAGAAATTTGATAGGAGGGATAATCAAGTTGAGAGAGTAGATATGCAGAAGAGGAATTGGCGTAATGATGGGAGGAAGAACAACAGAGAGACTGATAGGCAGCCGCAACAGTCAGAGAGGCCACCATCACCAGAGACCTGGCGGAAGCCTGAACAGCCAAAACCATCATCTCCTGATGGTGCTGGCCTGCATCACGGAAAAGCAGCTTCAGCTCTTGAGCTTGCGCAAGCATTTTCCAGATCAGTCTCAGATCCAAAATTAAACGATCGAATTTCTGGCCAAAGGGGAATTCCTAGCCGCGGTCAAATACCTTTTTCACGGCTGATGGGCCCGAGTCCCAGGCCCCAGATAAATGGTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FA_hydroxylase

Protein Analysis

657

Amino Acids

72.56

Weight (kDa)

9.58

Isoelectric Point (pI)

44.25

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001087)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34770 AT4G20870 AT4G20870
fragaria_vesca	FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10760 FvH4_3g10760 FvH4_3g10760 FvH4_3g10780 FvH4_3g10780 FvH4_3g10780
malus_domestica	MD05G1268900.v1.1
prunus_persica	Prupe.4G095700_v2.0.a1 Prupe.4G095700_v2.0.a1 Prupe.4G095700_v2.0.a1
pyrus_communis	pycom05g25190 pycom10g20540
rosa_chinensis	RchiOBHm_Chr5g0017441 RchiOBHm_Chr5g0017481 RchiOBHm_Chr5g0017491 RchiOBHm_Chr5g0017501
rosa_laevigata	RLG00000032287 RLG00000032288 RLG00000032292 RLG00000032294
rosa_multiflora	Rmu_sc0007392.1_g000004 Rmu_sc0013973.1_g000006
rosa_roxburghii	Rroxscaffold_1G00059930 Rroxscaffold_1G00059950
rosa_rugosa	Rorug05G0035400 Rorug05G0035500 Rorug05G0035800 Rorug05G0035900 Rorug05G0036000 Rorug05G0036100 Rorug05G0036200 Rorug05G0036300 Rorug05G0036400 Rorug05G0036500 Rorug05G0036600
rosa_samantha	Rh5AG130200 Rh5AG130400 Rh5BG128400 Rh5BG128600 Rh5CG140900 Rh5DG130400 Rh5DG130600 Rh5DG130700 Rh5DG130800 Rh7BG398200
rosa_wichuraiana	Rw5G011240 Rw5G011250 Rw5G011260 Rw5G011270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	317
Acc16I	TGCGCA	1 cut(s)	1836
Acc36I	ACCTGC	1 cut(s)	1048
AccB1I	GGYRCC	2 cut(s)	301, 415
AccB7I	CCANNNNNTGG	1 cut(s)	290
AccBSI	CCGCTC	2 cut(s)	21, 796
AccI	GTMKAC	1 cut(s)	841
AccII	CGCG	3 cut(s)	307, 470, 1911
AccIII	TCCGGA	1 cut(s)	599
AclWI	GGATC	2 cut(s)	123, 1856
AcoI	YGGCCR	5 cut(s)	134, 138, 182, 1281, 1888
AcsI	RAATTY	5 cut(s)	1237, 1303, 1616, 1881, 1899
AcuI	CTGAAG	3 cut(s)	1077, 1092, 1803
AcyI	GRCGYC	1 cut(s)	302
AdeI	CACNNNGTG	1 cut(s)	244
AfaI	GTAC	2 cut(s)	741, 1187
AfiI	CCNNNNNNNGG	4 cut(s)	204, 290, 691, 1910
AflII	CTTAAG	1 cut(s)	1403
AflIII	ACRYGT	1 cut(s)	1099
AgeI	ACCGGT	1 cut(s)	364
AgsI	TTSAA	6 cut(s)	681, 833, 1037, 1208, 1303, 1331
AjnI	CCWGG	8 cut(s)	203, 379, 428, 1249, 1484, 1543, 1748, 1949
AjuI	GAANNNNNNNTTGG	4 cut(s)	1149, 1181, 1884, 1916
AluBI	AGCT	7 cut(s)	236, 722, 1160, 1580, 1817, 1823, 1831
AluI	AGCT	7 cut(s)	236, 722, 1160, 1580, 1817, 1823, 1831
Alw21I	GWGCWC	4 cut(s)	249, 532, 640, 1384
Alw26I	GTCTC	5 cut(s)	198, 1323, 1694, 1739, 1861
Alw44I	GTGCAC	2 cut(s)	245, 636
AlwI	GGATC	2 cut(s)	123, 1856
AlwNI	CAGNNNCTG	1 cut(s)	1265
Ama87I	CYCGRG	1 cut(s)	1942
Aor13HI	TCCGGA	1 cut(s)	599
ApaI	GGGCCC	2 cut(s)	982, 1943
ApaLI	GTGCAC	2 cut(s)	245, 636
ApeKI	GCWGC	4 cut(s)	410, 1078, 1711, 1814
ApoI	RAATTY	5 cut(s)	1237, 1303, 1616, 1881, 1899
AsiGI	ACCGGT	1 cut(s)	364
Asp700I	GAANNNNTTC	1 cut(s)	1398
AspLEI	GCGC	4 cut(s)	304, 309, 1110, 1837
AsuC2I	CCSGG	3 cut(s)	93, 123, 181
AsuHPI	GGTGA	4 cut(s)	164, 451, 1181, 1731
AvaI	CYCGRG	1 cut(s)	1942
AvaII	GGWCC	3 cut(s)	597, 905, 1147
BaeGI	GKGCMC	4 cut(s)	249, 640, 982, 1943
BaeI	ACNNNNGTAYC	2 cut(s)	1169, 1202
BalI	TGGCCA	1 cut(s)	1890
BanI	GGYRCC	2 cut(s)	301, 415
BanII	GRGCYC	3 cut(s)	938, 982, 1943
BauI	CACGAG	1 cut(s)	1389
BbsI	GAAGAC	1 cut(s)	645
Bbv12I	GWGCWC	4 cut(s)	249, 532, 640, 1384
BbvI	GCAGC	4 cut(s)	397, 1090, 1723, 1826
BceAI	ACGGC	3 cut(s)	67, 1268, 1946
BciT130I	CCWGG	8 cut(s)	205, 381, 430, 1251, 1486, 1545, 1750, 1951
BclI	TGATCA	3 cut(s)	1171, 1431, 1597
BcnI	CCSGG	3 cut(s)	93, 123, 181
BcoDI	GTCTC	5 cut(s)	198, 1323, 1694, 1739, 1861
BfaI	CTAG	7 cut(s)	233, 633, 714, 900, 975, 1905, 1972
BfoI	RGCGCY	2 cut(s)	305, 1111
BfrI	CTTAAG	1 cut(s)	1403
BfuAI	ACCTGC	1 cut(s)	1048
BglI	GCCNNNNNGGC	1 cut(s)	818
BlpI	GCTNAGC	1 cut(s)	531
Bme18I	GGWCC	3 cut(s)	597, 905, 1147
BmeT110I	CYCGRG	1 cut(s)	1942
BmsI	GCATC	5 cut(s)	383, 799, 1330, 1483, 1810
BpiI	GAAGAC	1 cut(s)	645
BplI	GAGNNNNNCTC	2 cut(s)	514, 546
BpmI	CTGGAG	1 cut(s)	1146
Bpu10I	CCTNAGC	1 cut(s)	1044
Bpu1102I	GCTNAGC	1 cut(s)	531
BpuEI	CTTGAG	3 cut(s)	23, 662, 1847
BpuMI	CCSGG	3 cut(s)	93, 123, 181
BsaAI	YACGTR	1 cut(s)	1102
BsaBI	GATNNNNATC	1 cut(s)	667
BsaHI	GRCGYC	1 cut(s)	302
BsaI	GGTCTC	2 cut(s)	1323, 1739
BsaWI	WCCGGW	2 cut(s)	364, 599
Bsc4I	CCNNNNNNNGG	4 cut(s)	204, 290, 691, 1910
Bse118I	RCCGGY	4 cut(s)	132, 136, 364, 580
Bse1I	ACTGG	7 cut(s)	291, 685, 1142, 1163, 1284, 1401, 1612
Bse8I	GATNNNNATC	1 cut(s)	667
BseAI	TCCGGA	1 cut(s)	599
BseBI	CCWGG	8 cut(s)	205, 381, 430, 1251, 1486, 1545, 1750, 1951
BseGI	GGATG	9 cut(s)	277, 295, 374, 410, 783, 787, 856, 1345, 1588
BseJI	GATNNNNATC	1 cut(s)	667
BseLI	CCNNNNNNNGG	4 cut(s)	204, 290, 691, 1910
BseMII	CTCAG	9 cut(s)	465, 500, 545, 567, 1058, 1257, 1334, 1572, 1872
BseNI	ACTGG	7 cut(s)	291, 685, 1142, 1163, 1284, 1401, 1612
BseSI	GKGCMC	4 cut(s)	249, 640, 982, 1943
BseX3I	CGGCCG	2 cut(s)	134, 138
BseXI	GCAGC	4 cut(s)	397, 1090, 1723, 1826
BseYI	CCCAGC	1 cut(s)	343
BsgI	GTGCAG	2 cut(s)	419, 1097
Bsh1236I	CGCG	3 cut(s)	307, 470, 1911
Bsh1285I	CGRYCG	4 cut(s)	133, 137, 141, 1879
BshNI	GGYRCC	2 cut(s)	301, 415
BshTI	ACCGGT	1 cut(s)	364
BsiEI	CGRYCG	4 cut(s)	133, 137, 141, 1879
BsiHKAI	GWGCWC	4 cut(s)	249, 532, 640, 1384
BsiHKCI	CYCGRG	1 cut(s)	1942
BslFI	GGGAC	3 cut(s)	409, 918, 1932
BslI	CCNNNNNNNGG	4 cut(s)	204, 290, 691, 1910
BsmAI	GTCTC	5 cut(s)	198, 1323, 1694, 1739, 1861
BsmBI	CGTCTC	1 cut(s)	198
BsmFI	GGGAC	3 cut(s)	409, 918, 1932
BsmI	GAATGC	4 cut(s)	330, 544, 1369, 1456
Bso31I	GGTCTC	2 cut(s)	1323, 1739
BsoBI	CYCGRG	1 cut(s)	1942
Bsp120I	GGGCCC	2 cut(s)	978, 1939
Bsp1286I	GDGCHC	7 cut(s)	249, 532, 640, 938, 982, 1384, 1943
Bsp13I	TCCGGA	1 cut(s)	599
Bsp1407I	TGTACA	1 cut(s)	739
Bsp143I	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
Bsp1720I	GCTNAGC	1 cut(s)	531
BspCNI	CTCAG	9 cut(s)	464, 499, 544, 568, 1057, 1258, 1333, 1573, 1871
BspEI	TCCGGA	1 cut(s)	599
BspFNI	CGCG	3 cut(s)	307, 470, 1911
BspMI	ACCTGC	1 cut(s)	1048
BspPI	GGATC	2 cut(s)	123, 1856
BspT107I	GGYRCC	2 cut(s)	301, 415
BspTI	CTTAAG	1 cut(s)	1403
BspTNI	GGTCTC	2 cut(s)	1323, 1739
BsrBI	CCGCTC	2 cut(s)	21, 796
BsrFI	RCCGGY	4 cut(s)	132, 136, 364, 580
BsrGI	TGTACA	1 cut(s)	739
BsrI	ACTGG	7 cut(s)	291, 685, 1142, 1163, 1284, 1401, 1612
BssAI	RCCGGY	4 cut(s)	132, 136, 364, 580
BssMI	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
BssNI	GRCGYC	1 cut(s)	302
BssSI	CACGAG	1 cut(s)	1389
BssT1I	CCWWGG	3 cut(s)	747, 816, 1505
Bst2BI	CACGAG	1 cut(s)	1389
Bst2UI	CCWGG	8 cut(s)	205, 381, 430, 1251, 1486, 1545, 1750, 1951
Bst4CI	ACNGT	5 cut(s)	176, 1018, 1213, 1317, 1722
Bst6I	CTCTTC	1 cut(s)	1655
BstACI	GRCGYC	1 cut(s)	302
BstAFI	CTTAAG	1 cut(s)	1403
BstAUI	TGTACA	1 cut(s)	739
BstBAI	YACGTR	1 cut(s)	1102
BstC8I	GCNNGC	9 cut(s)	138, 281, 398, 1261, 1384, 1795, 1799, 1833, 1839
BstDSI	CCRYGG	3 cut(s)	1284, 1313, 1909
BstF5I	GGATG	9 cut(s)	277, 295, 374, 410, 783, 787, 856, 1345, 1588
BstFNI	CGCG	3 cut(s)	307, 470, 1911
BstH2I	RGCGCY	2 cut(s)	305, 1111
BstHHI	GCGC	4 cut(s)	304, 309, 1110, 1837
BstKTI	GATC	9 cut(s)	73, 118, 172, 1174, 1434, 1600, 1853, 1864, 1879
BstMAI	GTCTC	5 cut(s)	198, 1323, 1694, 1739, 1861
BstMBI	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
BstMCI	CGRYCG	4 cut(s)	133, 137, 141, 1879
BstMWI	GCNNNNNNNGC	7 cut(s)	253, 327, 673, 719, 818, 1765, 1820
BstNI	CCWGG	8 cut(s)	205, 381, 430, 1251, 1486, 1545, 1750, 1951
BstNSI	RCATGY	1 cut(s)	1553
BstSLI	GKGCMC	4 cut(s)	249, 640, 982, 1943
BstUI	CGCG	3 cut(s)	307, 470, 1911
BstV1I	GCAGC	4 cut(s)	397, 1090, 1723, 1826
BstV2I	GAAGAC	1 cut(s)	645
BstX2I	RGATCY	1 cut(s)	1861
BstXI	CCANNNNNNTGG	2 cut(s)	1749, 1964
BstYI	RGATCY	1 cut(s)	1861
BstZI	CGGCCG	2 cut(s)	134, 138
BtgI	CCRYGG	3 cut(s)	1284, 1313, 1909
BtgZI	GCGATG	1 cut(s)	336
BtsCI	GGATG	9 cut(s)	277, 295, 374, 410, 783, 787, 856, 1345, 1588
BtsIMutI	CAGTG	2 cut(s)	284, 1149
BveI	ACCTGC	1 cut(s)	1048
Cac8I	GCNNGC	9 cut(s)	138, 281, 398, 1261, 1384, 1795, 1799, 1833, 1839
CaiI	CAGNNNCTG	1 cut(s)	1265
CfoI	GCGC	4 cut(s)	304, 309, 1110, 1837
Cfr10I	RCCGGY	4 cut(s)	132, 136, 364, 580
Cfr42I	CCGCGG	1 cut(s)	1912
CseI	GACGC	4 cut(s)	43, 476, 773, 1280
CsiI	ACCWGGT	1 cut(s)	1249
Csp6I	GTAC	2 cut(s)	740, 1186
CspAI	ACCGGT	1 cut(s)	364
CspCI	CAANNNNNGTGG	2 cut(s)	1125, 1160
CviAII	CATG	7 cut(s)	543, 594, 629, 1175, 1478, 1499, 1550
CviQI	GTAC	2 cut(s)	740, 1186
DinI	GGCGCC	1 cut(s)	303
DpnI	GATC	9 cut(s)	72, 117, 171, 1173, 1433, 1599, 1852, 1863, 1878
DpnII	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
DraI	TTTAAA	1 cut(s)	1308
DraIII	CACNNNGTG	1 cut(s)	244
EaeI	YGGCCR	5 cut(s)	134, 138, 182, 1281, 1888
EagI	CGGCCG	2 cut(s)	134, 138
Eam1104I	CTCTTC	1 cut(s)	1655
EarI	CTCTTC	1 cut(s)	1655
EciI	GGCGGA	2 cut(s)	271, 1768
EclXI	CGGCCG	2 cut(s)	134, 138
Eco130I	CCWWGG	3 cut(s)	747, 816, 1505
Eco24I	GRGCYC	3 cut(s)	938, 982, 1943
Eco31I	GGTCTC	2 cut(s)	1323, 1739
Eco47I	GGWCC	3 cut(s)	597, 905, 1147
Eco52I	CGGCCG	2 cut(s)	134, 138
Eco57I	CTGAAG	3 cut(s)	1077, 1092, 1803
Eco88I	CYCGRG	1 cut(s)	1942
EcoO109I	RGGNCCY	4 cut(s)	905, 978, 979, 1953
EcoRI	GAATTC	2 cut(s)	1237, 1899
EcoRII	CCWGG	8 cut(s)	203, 379, 428, 1249, 1484, 1543, 1748, 1949
EcoT14I	CCWWGG	3 cut(s)	747, 816, 1505
EcoT22I	ATGCAT	1 cut(s)	544
EcoT38I	GRGCYC	3 cut(s)	938, 982, 1943
EgeI	GGCGCC	1 cut(s)	303
EheI	GGCGCC	1 cut(s)	303
ErhI	CCWWGG	3 cut(s)	747, 816, 1505
Esp3I	CGTCTC	1 cut(s)	198
FaeI	CATG	7 cut(s)	546, 597, 632, 1178, 1481, 1502, 1553
FaqI	GGGAC	3 cut(s)	409, 918, 1932
FatI	CATG	7 cut(s)	542, 593, 628, 1174, 1477, 1498, 1549
FauI	CCCGC	2 cut(s)	205, 559
FbaI	TGATCA	3 cut(s)	1171, 1431, 1597
FblI	GTMKAC	1 cut(s)	841
FokI	GGATG	9 cut(s)	284, 302, 361, 417, 790, 794, 863, 1352, 1575
FriOI	GRGCYC	3 cut(s)	938, 982, 1943
FseI	GGCCGGCC	1 cut(s)	140
FspBI	CTAG	7 cut(s)	233, 633, 714, 900, 975, 1905, 1972
FspI	TGCGCA	1 cut(s)	1836
GlaI	GCGC	4 cut(s)	303, 308, 1109, 1836
GsaI	CCCAGC	1 cut(s)	347
GsuI	CTGGAG	1 cut(s)	1146
HaeII	RGCGCY	2 cut(s)	305, 1111
HgaI	GACGC	4 cut(s)	43, 476, 773, 1280
HhaI	GCGC	4 cut(s)	304, 309, 1110, 1837
Hin1I	GRCGYC	1 cut(s)	302
Hin1II	CATG	7 cut(s)	546, 597, 632, 1178, 1481, 1502, 1553
Hin6I	GCGC	4 cut(s)	302, 307, 1108, 1835
HinP1I	GCGC	4 cut(s)	302, 307, 1108, 1835
HinfI	GANTC	4 cut(s)	223, 859, 1585, 1945
HphI	GGTGA	4 cut(s)	164, 451, 1181, 1731
Hpy166II	GTNNAC	5 cut(s)	247, 638, 740, 842, 1147
Hpy188III	TCNNGA	5 cut(s)	600, 1328, 1784, 1826, 1848
Hpy8I	GTNNAC	5 cut(s)	247, 638, 740, 842, 1147
HpyAV	CCTTC	6 cut(s)	93, 550, 640, 687, 868, 943
HpyCH4III	ACNGT	5 cut(s)	176, 1018, 1213, 1317, 1722
HpyCH4IV	ACGT	2 cut(s)	191, 1101
HpyF10VI	GCNNNNNNNGC	7 cut(s)	253, 327, 673, 719, 818, 1765, 1820
HpySE526I	ACGT	2 cut(s)	191, 1101
Hsp92I	GRCGYC	1 cut(s)	302
Hsp92II	CATG	7 cut(s)	546, 597, 632, 1178, 1481, 1502, 1553
HspAI	GCGC	4 cut(s)	302, 307, 1108, 1835
KasI	GGCGCC	1 cut(s)	301
KflI	GGGWCCC	1 cut(s)	905
Kpn2I	TCCGGA	1 cut(s)	599
KroI	GCCGGC	1 cut(s)	136
KroNI	GCCGGC	1 cut(s)	138
Ksp22I	TGATCA	3 cut(s)	1171, 1431, 1597
KspI	CCGCGG	1 cut(s)	1912
Kzo9I	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
LmnI	GCTCC	5 cut(s)	94, 933, 1165, 1288, 1408
Lsp1109I	GCAGC	4 cut(s)	397, 1090, 1723, 1826
LweI	GCATC	5 cut(s)	383, 799, 1330, 1483, 1810
MabI	ACCWGGT	1 cut(s)	1249
MaeI	CTAG	7 cut(s)	233, 633, 714, 900, 975, 1905, 1972
MaeII	ACGT	2 cut(s)	191, 1101
MaeIII	GTNAC	8 cut(s)	125, 464, 571, 761, 954, 1025, 1122, 1967
MalI	GATC	9 cut(s)	72, 117, 171, 1173, 1433, 1599, 1852, 1863, 1878
MbiI	CCGCTC	2 cut(s)	21, 796
MboI	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
MboII	GAAGA	6 cut(s)	19, 22, 645, 1083, 1672, 1699
MfeI	CAATTG	2 cut(s)	1334, 1344
MflI	RGATCY	1 cut(s)	1861
MhlI	GDGCHC	7 cut(s)	249, 532, 640, 938, 982, 1384, 1943
MlsI	TGGCCA	1 cut(s)	1890
MluNI	TGGCCA	1 cut(s)	1890
Mly113I	GGCGCC	1 cut(s)	302
MlyI	GAGTC	2 cut(s)	217, 1954
Mox20I	TGGCCA	1 cut(s)	1890
Mph1103I	ATGCAT	1 cut(s)	544
MroI	TCCGGA	1 cut(s)	599
MroNI	GCCGGC	1 cut(s)	136
MroXI	GAANNNNTTC	1 cut(s)	1398
MscI	TGGCCA	1 cut(s)	1890
MseI	TTAA	5 cut(s)	480, 1118, 1307, 1404, 1871
MslI	CAYNNNNRTG	3 cut(s)	213, 335, 633
Msp20I	TGGCCA	1 cut(s)	1890
MspA1I	CMGCKG	2 cut(s)	722, 1911
MspCI	CTTAAG	1 cut(s)	1403
MteI	GCGCNGCGC	1 cut(s)	305
MunI	CAATTG	2 cut(s)	1334, 1344
Mva1269I	GAATGC	4 cut(s)	330, 544, 1369, 1456
MvaI	CCWGG	8 cut(s)	205, 381, 430, 1251, 1486, 1545, 1750, 1951
MvnI	CGCG	3 cut(s)	307, 470, 1911
MwoI	GCNNNNNNNGC	7 cut(s)	253, 327, 673, 719, 818, 1765, 1820
NaeI	GCCGGC	1 cut(s)	138
NarI	GGCGCC	1 cut(s)	302
NciI	CCSGG	3 cut(s)	93, 123, 181
NdeII	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
NgoMIV	GCCGGC	1 cut(s)	136
NlaIII	CATG	7 cut(s)	546, 597, 632, 1178, 1481, 1502, 1553
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	2 cut(s)	464, 761
NsbI	TGCGCA	1 cut(s)	1836
NsiI	ATGCAT	1 cut(s)	544
NspI	RCATGY	1 cut(s)	1553
PasI	CCCWGGG	2 cut(s)	380, 429
PctI	GAATGC	4 cut(s)	330, 544, 1369, 1456
PdiI	GCCGGC	1 cut(s)	138
PdmI	GAANNNNTTC	1 cut(s)	1398
PfeI	GAWTC	2 cut(s)	859, 1585
PflMI	CCANNNNNTGG	1 cut(s)	290
PinAI	ACCGGT	1 cut(s)	364
Ple19I	CGATCG	1 cut(s)	1879
PleI	GAGTC	2 cut(s)	217, 1953
PluTI	GGCGCC	1 cut(s)	305
PpsI	GAGTC	2 cut(s)	217, 1953
Ppu21I	YACGTR	1 cut(s)	1102
PpuMI	RGGWCCY	1 cut(s)	905
PsiI	TTATAA	1 cut(s)	317
Psp5II	RGGWCCY	1 cut(s)	905
Psp6I	CCWGG	8 cut(s)	203, 379, 428, 1249, 1484, 1543, 1748, 1949
PspFI	CCCAGC	1 cut(s)	343
PspGI	CCWGG	8 cut(s)	203, 379, 428, 1249, 1484, 1543, 1748, 1949
PspOMI	GGGCCC	2 cut(s)	978, 1939
PspPPI	RGGWCCY	1 cut(s)	905
PstNI	CAGNNNCTG	1 cut(s)	1265
PsuI	RGATCY	1 cut(s)	1861
PvuI	CGATCG	1 cut(s)	1879
PvuII	CAGCTG	1 cut(s)	722
RigI	GGCCGGCC	1 cut(s)	140
RsaI	GTAC	2 cut(s)	741, 1187
RsaNI	GTAC	2 cut(s)	740, 1186
RseI	CAYNNNNRTG	3 cut(s)	213, 335, 633
SacII	CCGCGG	1 cut(s)	1912
SaqAI	TTAA	5 cut(s)	480, 1118, 1307, 1404, 1871
Sau3AI	GATC	9 cut(s)	70, 115, 169, 1171, 1431, 1597, 1850, 1861, 1876
SchI	GAGTC	2 cut(s)	217, 1954
SduI	GDGCHC	7 cut(s)	249, 532, 640, 938, 982, 1384, 1943
SexAI	ACCWGGT	1 cut(s)	1249
SfaNI	GCATC	5 cut(s)	383, 799, 1330, 1483, 1810
SfoI	GGCGCC	1 cut(s)	303
Sfr303I	CCGCGG	1 cut(s)	1912
SgrBI	CCGCGG	1 cut(s)	1912
SinI	GGWCC	3 cut(s)	597, 905, 1147
SmiMI	CAYNNNNRTG	3 cut(s)	213, 335, 633
SmlI	CTYRAG	4 cut(s)	38, 641, 1403, 1826
SmoI	CTYRAG	4 cut(s)	38, 641, 1403, 1826
SspDI	GGCGCC	1 cut(s)	301
SspMI	CTAG	7 cut(s)	233, 633, 714, 900, 975, 1905, 1972
StyI	CCWWGG	3 cut(s)	747, 816, 1505
TaaI	ACNGT	5 cut(s)	176, 1018, 1213, 1317, 1722
TaiI	ACGT	2 cut(s)	194, 1104
TaqI	TCGA	3 cut(s)	5, 705, 1879
TatI	WGTACW	1 cut(s)	739
TauI	GCSGC	7 cut(s)	143, 166, 307, 416, 799, 1717, 1911
TfiI	GAWTC	2 cut(s)	859, 1585
Tru1I	TTAA	5 cut(s)	480, 1118, 1307, 1404, 1871
Tru9I	TTAA	5 cut(s)	480, 1118, 1307, 1404, 1871
TscAI	CASTG	2 cut(s)	291, 1149
TseFI	GTSAC	2 cut(s)	464, 761
TseI	GCWGC	4 cut(s)	410, 1078, 1711, 1814
Tsp45I	GTSAC	2 cut(s)	464, 761
TspDTI	ATGAA	3 cut(s)	288, 1233, 1250
TspGWI	ACGGA	3 cut(s)	128, 216, 1821
TspRI	CASTG	2 cut(s)	291, 1149
Van91I	CCANNNNNTGG	1 cut(s)	290
Vha464I	CTTAAG	1 cut(s)	1403
VneI	GTGCAC	2 cut(s)	245, 636
VpaK11BI	GGWCC	3 cut(s)	597, 905, 1147
XapI	RAATTY	5 cut(s)	1237, 1303, 1616, 1881, 1899
XceI	RCATGY	1 cut(s)	1553
XmiI	GTMKAC	1 cut(s)	841
XmnI	GAANNNNTTC	1 cut(s)	1398
XspI	CTAG	7 cut(s)	233, 633, 714, 900, 975, 1905, 1972
Zsp2I	ATGCAT	1 cut(s)	544

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.