Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0001087

Physical Location & Seq

Genomic Coordinates Forward (+)

2645644 .. 2647889

Gene Structure 2246 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0036600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGCATTGTTCTGTGATCTCCAAGTCGATGTCAATGGCGAAGAAGTCTTCATGGTGAACAAGGAAACTTTGGCATCTTTCTCTTGTCGATTAAGCAAACTATTTGGTAAATCAATGGGTACAACGAGCCTCAAAGTGATATTCCATGACTTTCCTGGAGGTGCAGAGGGTTTTGAGCTCATGACAAGGTTTTGTTACAACAATGGAACAACCGAAATAACTCCTTCCAACTTAGTCCTTGTATACTGCATCGCCGATTTCATGGAGATGGATGGTGATATACTATTGCAAGCTCAAAACACTCTTGATGGTATCAGCTCTTGGACGTGGTCTGAGCTTCTAGTAGCTCTGAAGCAGTGCCAAGATTTACTTTCCCCCTCAAATTCTTCATTGATATTGCAGATAGTCATGGACTGCATCATAGGCAAGCTTTCTTGTCCATTTTTTCCAACCCCCTATAATCTTAAAAGCAATTGCTCTATGAAAACATGGTGGTTTGAAGACTTGATGTTCTTAAATACAAAATCGATCGAAATGGTTATAAGAAGCCTGATATCCAAGGAGATTGAACAGTCTACAGTTTTCAGGTTTCTCCTTCGTTATCATCAGTCAAAGTGTTTTGGTGCCAAACCAGAAGAGAAGTACAAGATCACAGAGGTTGTTATCGGTCTACTTTCTCTGCTTGATAGGAGCTTCCTTTCTTTCAAGGGATTATTCAATATATATCAAGCGGCTTTGAACATGAAAGTAGGCAAGAAATACAAAAACAAGTTGGAGATTATGATAGGTTCACAGCTGGATCAAGCAACAATCGATTATTTACTTGTCCCATCTCCCTGTGGGAAAAAGTATGTTTATGATGTGAATTTGATTTTGAGGTTAGGGAAATCATTTCTTCTTCAAGAAGGGGGTCATTTATCCCAGAAGAGTCACTTCACAAAGGTCGCCAAGTTGATGGATTTATACCTTGCAGAAGTGGCTCCAGATTTTCATTTGAAACCTTCAAAGTTTGCAGCATTAGTTATGATGTTGCCAGCGTCTGTGCGAGAATCTCATGACAGAATTTACGATGCCATTGCTGTATATTTTAAGTGCCACCCTGGTCTATCTGAACAAGAGAAGCTGACAATCTGTTGTGCACTGAACTATAAGAAGCTCTCAGCAGGACCTTTGAAACAAGCCTCTCACACAAAGTTCCCTTCCAGAAGAGCAGTTGAAGCTTTCAAGAAACAGCGATCCAAGCTCAGAAGCGTACTCCAGGAAGTCTACTACATCCGAACCAAGGATAAGAAAGAGGAAATTGAGTCAGTCCTACTTGACGCAAAGAGTCTTGATCTCCCAACAGAGGCTCAGAAGCTCAGAGCAGATTTGCAAAGGATGCATTCGAAGGCATTGGAATTGGGGAAGGTTTTCGGGACAATGCAGATACAAGTGACAAGTGTAAACAAATCAAGATTGCCCTTTCAGGCAACCAATACTCGATACTTGCCAAAACTCTTTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FA_hydroxylase

Protein Analysis

501

Amino Acids

56.94

Weight (kDa)

9.04

Isoelectric Point (pI)

41.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPH3	PF03000	163 - 388	7.8e-54	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001087)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34770 AT4G20870 AT4G20870
fragaria_vesca	FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10720 FvH4_3g10760 FvH4_3g10760 FvH4_3g10760 FvH4_3g10780 FvH4_3g10780 FvH4_3g10780
malus_domestica	MD05G1268900.v1.1
prunus_persica	Prupe.4G095700_v2.0.a1 Prupe.4G095700_v2.0.a1 Prupe.4G095700_v2.0.a1
pyrus_communis	pycom05g25190 pycom10g20540
rosa_chinensis	RchiOBHm_Chr5g0017441 RchiOBHm_Chr5g0017481 RchiOBHm_Chr5g0017491 RchiOBHm_Chr5g0017501
rosa_laevigata	RLG00000032287 RLG00000032288 RLG00000032292 RLG00000032294
rosa_multiflora	Rmu_sc0007392.1_g000004 Rmu_sc0013973.1_g000006
rosa_roxburghii	Rroxscaffold_1G00059930 Rroxscaffold_1G00059950
rosa_rugosa	Rorug05G0035400 Rorug05G0035500 Rorug05G0035800 Rorug05G0035900 Rorug05G0036000 Rorug05G0036100 Rorug05G0036200 Rorug05G0036300 Rorug05G0036400 Rorug05G0036500 Rorug05G0036600
rosa_samantha	Rh5AG130200 Rh5AG130400 Rh5BG128400 Rh5BG128600 Rh5CG140900 Rh5DG130400 Rh5DG130600 Rh5DG130700 Rh5DG130800 Rh7BG398200
rosa_wichuraiana	Rw5G011240 Rw5G011250 Rw5G011260 Rw5G011270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	542
AccB1I	GGYRCC	1 cut(s)	623
AccI	GTMKAC	4 cut(s)	243, 575, 670, 1266
AciI	CCGC	1 cut(s)	731
AclWI	GGATC	2 cut(s)	807, 1230
AcsI	RAATTY	3 cut(s)	382, 865, 1062
AcuI	CTGAAG	1 cut(s)	371
AfaI	GTAC	3 cut(s)	121, 644, 1254
AfiI	CCNNNNNNNGG	2 cut(s)	1282, 1345
AjiI	CACGTC	1 cut(s)	327
AjnI	CCWGG	3 cut(s)	154, 1099, 1257
Alw21I	GWGCWC	2 cut(s)	180, 1141
Alw44I	GTGCAC	1 cut(s)	1137
AlwI	GGATC	2 cut(s)	807, 1230
AlwNI	CAGNNNCTG	1 cut(s)	1040
ApaLI	GTGCAC	1 cut(s)	1137
ApeKI	GCWGC	1 cut(s)	1013
ApoI	RAATTY	3 cut(s)	382, 865, 1062
Asp700I	GAANNNNTTC	1 cut(s)	1409
AspS9I	GGNCC	1 cut(s)	1166
AsuHPI	GGTGA	2 cut(s)	67, 287
AsuII	TTCGAA	1 cut(s)	1385
AvaII	GGWCC	1 cut(s)	1166
BaeGI	GKGCMC	1 cut(s)	1141
BanI	GGYRCC	1 cut(s)	623
BanII	GRGCYC	1 cut(s)	180
BbsI	GAAGAC	2 cut(s)	40, 507
Bbv12I	GWGCWC	2 cut(s)	180, 1141
BbvI	GCAGC	1 cut(s)	1025
BccI	CCATC	5 cut(s)	262, 266, 302, 838, 949
BcgI	CGANNNNNNTGC	2 cut(s)	1058, 1092
BciT130I	CCWGG	3 cut(s)	156, 1101, 1259
BfaI	CTAG	1 cut(s)	341
BfmI	CTRYAG	1 cut(s)	576
BisI	GCNGC	2 cut(s)	732, 1014
BlsI	GCNGC	2 cut(s)	733, 1015
Bme1390I	CCNGG	3 cut(s)	156, 1101, 1259
Bme18I	GGWCC	1 cut(s)	1166
BmgBI	CACGTC	1 cut(s)	327
BmgT120I	GGNCC	1 cut(s)	1166
BmiI	GGNNCC	2 cut(s)	625, 981
BmrFI	CCNGG	3 cut(s)	156, 1101, 1259
BmsI	GCATC	5 cut(s)	83, 258, 426, 1060, 1368
BpiI	GAAGAC	2 cut(s)	40, 507
BpmI	CTGGAG	3 cut(s)	177, 966, 1241
Bpu14I	TTCGAA	1 cut(s)	1385
Bsa29I	ATCGAT	2 cut(s)	527, 813
BsaJI	CCNNGG	3 cut(s)	558, 1099, 1281
Bsc4I	CCNNNNNNNGG	2 cut(s)	1282, 1345
Bse3DI	GCAATG	1 cut(s)	1074
BseBI	CCWGG	3 cut(s)	156, 1101, 1259
BseCI	ATCGAT	2 cut(s)	527, 813
BseDI	CCNNGG	3 cut(s)	558, 1099, 1281
BseGI	GGATG	3 cut(s)	277, 1272, 1383
BseLI	CCNNNNNNNGG	2 cut(s)	1282, 1345
BseMI	GCAATG	1 cut(s)	1074
BseMII	CTCAG	5 cut(s)	324, 1173, 1258, 1364, 1372
BseSI	GKGCMC	1 cut(s)	1141
BseXI	GCAGC	1 cut(s)	1025
BsgI	GTGCAG	1 cut(s)	183
Bsh1285I	CGRYCG	1 cut(s)	531
BshNI	GGYRCC	1 cut(s)	623
BshVI	ATCGAT	2 cut(s)	527, 813
BsiEI	CGRYCG	1 cut(s)	531
BsiHKAI	GWGCWC	2 cut(s)	180, 1141
BslFI	GGGAC	2 cut(s)	812, 1429
BslI	CCNNNNNNNGG	2 cut(s)	1282, 1345
BsmFI	GGGAC	2 cut(s)	812, 1429
BsmI	GAATGC	1 cut(s)	1381
Bsp119I	TTCGAA	1 cut(s)	1385
Bsp1286I	GDGCHC	2 cut(s)	180, 1141
Bsp143I	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
BspACI	CCGC	1 cut(s)	731
BspCNI	CTCAG	5 cut(s)	325, 1172, 1257, 1363, 1371
BspDI	ATCGAT	2 cut(s)	527, 813
BspHI	TCATGA	2 cut(s)	180, 1054
BspLI	GGNNCC	2 cut(s)	625, 981
BspPI	GGATC	2 cut(s)	807, 1230
BspQI	GCTCTTC	1 cut(s)	1201
BspT104I	TTCGAA	1 cut(s)	1385
BspT107I	GGYRCC	1 cut(s)	623
BsrDI	GCAATG	1 cut(s)	1074
BssECI	CCNNGG	3 cut(s)	558, 1099, 1281
BssMI	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
BssNAI	GTATAC	1 cut(s)	244
BssT1I	CCWWGG	2 cut(s)	558, 1281
Bst1107I	GTATAC	1 cut(s)	244
Bst2UI	CCWGG	3 cut(s)	156, 1101, 1259
Bst4CI	ACNGT	2 cut(s)	573, 580
Bst6I	CTCTTC	3 cut(s)	630, 920, 1201
BstAPI	GCANNNNNTGC	1 cut(s)	1378
BstBI	TTCGAA	1 cut(s)	1385
BstC8I	GCNNGC	3 cut(s)	291, 428, 1035
BstDEI	CTNAG	6 cut(s)	232, 333, 1159, 1244, 1350, 1358
BstF5I	GGATG	3 cut(s)	277, 1272, 1383
BstKTI	GATC	6 cut(s)	19, 531, 651, 802, 1238, 1336
BstMBI	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
BstMCI	CGRYCG	1 cut(s)	531
BstMWI	GCNNNNNNNGC	5 cut(s)	423, 977, 1217, 1240, 1378
BstNI	CCWGG	3 cut(s)	156, 1101, 1259
BstSCI	CCNGG	3 cut(s)	154, 1099, 1257
BstSFI	CTRYAG	1 cut(s)	576
BstSLI	GKGCMC	1 cut(s)	1141
BstV1I	GCAGC	1 cut(s)	1025
BstV2I	GAAGAC	2 cut(s)	40, 507
BstXI	CCANNNNNNTGG	1 cut(s)	955
BstZ17I	GTATAC	1 cut(s)	244
Bsu15I	ATCGAT	2 cut(s)	527, 813
BsuTUI	ATCGAT	2 cut(s)	527, 813
BtgZI	GCGATG	1 cut(s)	235
BtrI	CACGTC	1 cut(s)	327
BtsCI	GGATG	3 cut(s)	277, 1272, 1383
BtsI	GCAGTG	1 cut(s)	362
BtsIMutI	CAGTG	2 cut(s)	362, 1139
Cac8I	GCNNGC	3 cut(s)	291, 428, 1035
CaiI	CAGNNNCTG	1 cut(s)	1040
CciI	TCATGA	2 cut(s)	180, 1054
Cfr13I	GGNCC	1 cut(s)	1166
ClaI	ATCGAT	2 cut(s)	527, 813
CseI	GACGC	2 cut(s)	1026, 1328
Csp6I	GTAC	3 cut(s)	120, 643, 1253
CviAII	CATG	8 cut(s)	52, 146, 181, 262, 409, 489, 742, 1055
CviQI	GTAC	3 cut(s)	120, 643, 1253
DdeI	CTNAG	6 cut(s)	232, 333, 1159, 1244, 1350, 1358
DpnI	GATC	6 cut(s)	18, 530, 650, 801, 1237, 1335
DpnII	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
Eam1104I	CTCTTC	3 cut(s)	630, 920, 1201
EarI	CTCTTC	3 cut(s)	630, 920, 1201
Ecl136II	GAGCTC	1 cut(s)	178
Eco130I	CCWWGG	2 cut(s)	558, 1281
Eco24I	GRGCYC	1 cut(s)	180
Eco32I	GATATC	1 cut(s)	555
Eco47I	GGWCC	1 cut(s)	1166
Eco53kI	GAGCTC	1 cut(s)	178
Eco57I	CTGAAG	1 cut(s)	371
EcoICRI	GAGCTC	1 cut(s)	178
EcoO109I	RGGNCCY	1 cut(s)	1166
EcoRII	CCWGG	3 cut(s)	154, 1099, 1257
EcoRV	GATATC	1 cut(s)	555
EcoT14I	CCWWGG	2 cut(s)	558, 1281
EcoT22I	ATGCAT	1 cut(s)	1383
EcoT38I	GRGCYC	1 cut(s)	180
ErhI	CCWWGG	2 cut(s)	558, 1281
FaeI	CATG	8 cut(s)	55, 149, 184, 265, 412, 492, 745, 1058
FalI	AAGNNNNNCTT	4 cut(s)	354, 386, 917, 949
FaqI	GGGAC	2 cut(s)	812, 1429
FatI	CATG	8 cut(s)	51, 145, 180, 261, 408, 488, 741, 1054
FblI	GTMKAC	4 cut(s)	243, 575, 670, 1266
Fnu4HI	GCNGC	2 cut(s)	732, 1014
FokI	GGATG	3 cut(s)	284, 1259, 1390
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	2 cut(s)	732, 1014
FspBI	CTAG	1 cut(s)	341
GluI	GCNGC	2 cut(s)	732, 1014
GsuI	CTGGAG	3 cut(s)	177, 966, 1241
HgaI	GACGC	2 cut(s)	1026, 1328
Hin1II	CATG	8 cut(s)	55, 149, 184, 265, 412, 492, 745, 1058
HindIII	AAGCTT	2 cut(s)	428, 1218
HinfI	GANTC	4 cut(s)	928, 1049, 1304, 1327
HphI	GGTGA	2 cut(s)	67, 287
Hpy166II	GTNNAC	8 cut(s)	58, 244, 576, 671, 791, 1139, 1267, 1444
Hpy188I	TCNGA	7 cut(s)	334, 351, 1111, 1247, 1277, 1353, 1361
Hpy8I	GTNNAC	8 cut(s)	58, 244, 576, 671, 791, 1139, 1267, 1444
HpyAV	CCTTC	7 cut(s)	234, 605, 899, 1011, 1209, 1381, 1399
HpyCH4III	ACNGT	2 cut(s)	573, 580
HpyCH4IV	ACGT	1 cut(s)	326
HpyF10VI	GCNNNNNNNGC	5 cut(s)	423, 977, 1217, 1240, 1378
HpyF3I	CTNAG	6 cut(s)	232, 333, 1159, 1244, 1350, 1358
HpySE526I	ACGT	1 cut(s)	326
Hsp92II	CATG	8 cut(s)	55, 149, 184, 265, 412, 492, 745, 1058
Kzo9I	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
LguI	GCTCTTC	1 cut(s)	1201
LmnI	GCTCC	2 cut(s)	690, 985
Lsp1109I	GCAGC	1 cut(s)	1025
LweI	GCATC	5 cut(s)	83, 258, 426, 1060, 1368
MaeI	CTAG	1 cut(s)	341
MaeII	ACGT	1 cut(s)	326
MaeIII	GTNAC	3 cut(s)	194, 929, 1432
MalI	GATC	6 cut(s)	18, 530, 650, 801, 1237, 1335
MboI	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
MboII	GAAGA	9 cut(s)	40, 53, 378, 512, 647, 887, 890, 937, 1218
MfeI	CAATTG	1 cut(s)	472
MhlI	GDGCHC	2 cut(s)	180, 1141
MluCI	AATT	6 cut(s)	382, 472, 865, 1062, 1299, 1397
MlyI	GAGTC	3 cut(s)	937, 1313, 1336
MmeI	TCCRAC	3 cut(s)	252, 473, 753
MnlI	CCTC	9 cut(s)	140, 152, 160, 388, 649, 870, 1192, 1288, 1339
Mph1103I	ATGCAT	1 cut(s)	1383
MroXI	GAANNNNTTC	1 cut(s)	1409
MseI	TTAA	4 cut(s)	92, 465, 515, 1089
MslI	CAYNNNNRTG	1 cut(s)	266
MspA1I	CMGCKG	1 cut(s)	796
MspR9I	CCNGG	3 cut(s)	156, 1101, 1259
MunI	CAATTG	1 cut(s)	472
Mva1269I	GAATGC	1 cut(s)	1381
MvaI	CCWGG	3 cut(s)	156, 1101, 1259
MwoI	GCNNNNNNNGC	5 cut(s)	423, 977, 1217, 1240, 1378
NdeII	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
NlaIII	CATG	8 cut(s)	55, 149, 184, 265, 412, 492, 745, 1058
NlaIV	GGNNCC	2 cut(s)	625, 981
NmuCI	GTSAC	2 cut(s)	929, 1432
NsiI	ATGCAT	1 cut(s)	1383
NspV	TTCGAA	1 cut(s)	1385
PagI	TCATGA	2 cut(s)	180, 1054
PciSI	GCTCTTC	1 cut(s)	1201
PctI	GAATGC	1 cut(s)	1381
PdmI	GAANNNNTTC	1 cut(s)	1409
PfeI	GAWTC	1 cut(s)	1049
PflFI	GACNNNGTC	1 cut(s)	328
PfoI	TCCNGGA	2 cut(s)	154, 1257
PkrI	GCNGC	2 cut(s)	733, 1015
Ple19I	CGATCG	1 cut(s)	531
PleI	GAGTC	3 cut(s)	936, 1312, 1335
PpsI	GAGTC	3 cut(s)	936, 1312, 1335
PpuMI	RGGWCCY	1 cut(s)	1166
PsiI	TTATAA	1 cut(s)	542
Psp124BI	GAGCTC	1 cut(s)	180
Psp5II	RGGWCCY	1 cut(s)	1166
Psp6I	CCWGG	3 cut(s)	154, 1099, 1257
PspGI	CCWGG	3 cut(s)	154, 1099, 1257
PspN4I	GGNNCC	2 cut(s)	625, 981
PspPI	GGNCC	1 cut(s)	1166
PspPPI	RGGWCCY	1 cut(s)	1166
PstNI	CAGNNNCTG	1 cut(s)	1040
PsyI	GACNNNGTC	1 cut(s)	328
PvuI	CGATCG	1 cut(s)	531
PvuII	CAGCTG	1 cut(s)	796
RsaI	GTAC	3 cut(s)	121, 644, 1254
RsaNI	GTAC	3 cut(s)	120, 643, 1253
RseI	CAYNNNNRTG	1 cut(s)	266
SacI	GAGCTC	1 cut(s)	180
SapI	GCTCTTC	1 cut(s)	1201
SaqAI	TTAA	4 cut(s)	92, 465, 515, 1089
SatI	GCNGC	2 cut(s)	732, 1014
Sau3AI	GATC	6 cut(s)	16, 528, 648, 799, 1235, 1333
Sau96I	GGNCC	1 cut(s)	1166
SchI	GAGTC	3 cut(s)	937, 1313, 1336
ScrFI	CCNGG	3 cut(s)	156, 1101, 1259
SduI	GDGCHC	2 cut(s)	180, 1141
SfaNI	GCATC	5 cut(s)	83, 258, 426, 1060, 1368
SfcI	CTRYAG	1 cut(s)	576
SfuI	TTCGAA	1 cut(s)	1385
SinI	GGWCC	1 cut(s)	1166
SmiMI	CAYNNNNRTG	1 cut(s)	266
Sse9I	AATT	6 cut(s)	382, 472, 865, 1062, 1299, 1397
SsiI	CCGC	1 cut(s)	731
SspMI	CTAG	1 cut(s)	341
SstI	GAGCTC	1 cut(s)	180
StyD4I	CCNGG	3 cut(s)	154, 1099, 1257
StyI	CCWWGG	2 cut(s)	558, 1281
TaaI	ACNGT	2 cut(s)	573, 580
TaiI	ACGT	1 cut(s)	329
TaqI	TCGA	7 cut(s)	27, 88, 527, 531, 813, 1385, 1480
TaqII	GACCGA	1 cut(s)	656
TasI	AATT	6 cut(s)	382, 472, 865, 1062, 1299, 1397
TatI	WGTACW	1 cut(s)	642
TauI	GCSGC	1 cut(s)	734
TfiI	GAWTC	1 cut(s)	1049
Tru1I	TTAA	4 cut(s)	92, 465, 515, 1089
Tru9I	TTAA	4 cut(s)	92, 465, 515, 1089
TscAI	CASTG	2 cut(s)	362, 1146
TseFI	GTSAC	2 cut(s)	929, 1432
TseI	GCWGC	1 cut(s)	1013
Tsp45I	GTSAC	2 cut(s)	929, 1432
TspDTI	ATGAA	6 cut(s)	40, 250, 378, 497, 758, 980
TspRI	CASTG	2 cut(s)	362, 1146
Tth111I	GACNNNGTC	1 cut(s)	328
VneI	GTGCAC	1 cut(s)	1137
VpaK11BI	GGWCC	1 cut(s)	1166
XapI	RAATTY	3 cut(s)	382, 865, 1062
XcmI	CCANNNNNNNNNTGG	1 cut(s)	152
XmiI	GTMKAC	4 cut(s)	243, 575, 670, 1266
XmnI	GAANNNNTTC	1 cut(s)	1409
XspI	CTAG	1 cut(s)	341
Zsp2I	ATGCAT	1 cut(s)	1383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.