Rorug05G0349700

UNC93-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004549
Physical Location & Seq
Reverse (-)
43415937 .. 43441332
25396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0349700.1

Sequence Viewer

Length: 3357 bp
ATGACTGTCATGACCCCTGCCCCTATTGATCAACCAGAGGACGAGGAGATGCTTGTTCCGGCCTCGGATTTGCCTGAAAACAATCACCAGCCCATGGATGTTGTTGCACAGCCTGAAAATGCTAATAATGTGGAGAACCAACCAGTAGAAGATCCTCCTTCATCGAGATTCACATGGAGAATTGACAACTTTTCTAGGATGAATACAAAGAAGCTTTATTCTGACATATTCGTTGTTGGTGGCTATAAATGGCGTGTGCTTATTTTTCCCAAGGGAAACAATGTGGATCATTTGTCTATGTATTTGGATGTTGCGGATTCGTCAAATTTGCCATATGGGTGGAGTAGATATGCACAATTTAGCTTGGGAGTTCTCAATCAAGTTCATACCAAGTATTCTGTACGAAAAGACACACAACATCAGTTCAATGCACGGGAAAGTGATTGGGGTTTCACATCCTTTATGCCTCTTGGTGAACTGTACGATCCCAGCAGAGGTTATCTCGTGAATGATGCGGTTATAATTGAAGCGGAAGTTCTCGTTCGTAGGGTTGTAGATTATTGGACATATGACTCAAAGAAGGAAACGGGGTATGTTGGGCTTAAGAACCAAGGAGCAACATGCTATATGAACTCTCTCCTGCAGACTCTGTATCATATTCCTTATTTTAGAAAGGCTGTTTACCATATGCCCACGACTGAGAATGATTTGCCATCACAAAGCATACCATTGGCTCTTCAGAGTCTATTTTACAAACTCCAGTACAGTGATAATAGTGTTGCAACAAAAGAGCTGACAAAGTCTTTTGGATGGGATACATATGATTCATTTATGCAGCATGATGTGCAAGAACTAAATAGGGTCTTGTGTGAAAAACTGGAGGACAAAATGAAGGGAACTGTTGTAGAGGGAACCATACAGAAGTTATTTGAAGGTCACCATATGAACTACATCGAATGCATTAATGTGGACTACAAATCTACAAGAAAGGAGTCATTTTATGACCTTCAGCTCGATGTCAAAGGGTGTCGTGATGTATATGCTTCTTTTGATAAGTATGTAGAAGTTGAACGTCTTGAGGGGGATAACAAGTATCATGCTGAACAGAATGGATTACAGGATGCTAAGAAAGGGGTTCTCTTCATTGACTTTCCACCTGTTCTTCAACTTCAACTGAAACGGTTTGAGTACGACTTTATGCGAGACACCATGGTGAAGATTAATGACCGTTATGAGTTCCCTTTGCAACTTGACCTTGATCGAGATAATGGAAAATATTTATCACCTGAAGCTGATAGGAGTGTTCGCAACCTTTACACACTTCACAGTGTATTGGTACACAGTGGTGGGGTGCATGGTGGACATTACTACGCCTTTATCAGACCGACGCTTTCTGATCAGTGGTACAAATTTGATGATGAGAGGGTAACAAAAGAAGATATAAAGAGGGCACTGGAGGAGCAGTATGGTGGTGAGGAAGAGCTACCGCAGACAAACCCTGGATTTAATAACACTCCTTTCAAATTTACAAAATACTCAAACGCGTACATGCTGGTGTACATACGTGAAAGTGACAAGGATAAAATAATCTGCAATGTTGATGAGAAGGACATTGCTGAACATCTACGGGAGAGGTTGAAGAAAGAACAAGAAGAAAAGGAGCACAAGAAGAAAGAAAAGGCAGAGGCACACCTATATACTATCATTAAGGTTGCCCGGGACGAGAACCTTCTGGAGCAGATTGGGAAGGATATATATTTTGATCTTGTGGATCATGACAAAGTCAAAAGTTTTCGTATTCAGAAGCAGATGCCTTTCAACCTTTTCAAGGAGGAGGTTGCTAAGGAGTTTGGCATACCAGTGCAATTTCAGCGGTTTTGGCTGTGGGCAAAGCGTCAAAACCATACATATCGTCCAAACCGTCCATTGACACCTGTGGAGGAAACACAATCTGTTGGGCAGTTGAGGGAGGTATCAAACAAGGTTCATAATGCAGAACTGAAGTTGTTCTTGGAAATAGAGCTTGGACCGGATTTACACCCTATTGCTCCACCTGACAAGACAAAGGATGATATTCTGCTTTTCTTTAAGCTTTATGATCCTGAAAAAGAAGAACTACGTTACGTTGGACGGCTTTTTGTGAAGAGTACTGGTAAGCCAGCTGAAATCTTGTCAAAATTAAATGAATTGGCTGGCTATGCCCCTGACGAGGAGATCGACCTTTACGAGGAAATTAAGTATGAGCCCACTGTCATGTGTGAACCCATCGATAAGAAATTCACATTTCGAGCCAGCCAGCTTGAGGATGGAGACATTGTTTGCTTCCAAAAACCAGTTCCGGTTGAAAATGGTCAGCAGTTCCGCTATCCAGACGTCCCATCTTTTCTGGATTTTGTGCACAATCGTCTCGTTGTTCATTTTCGATCGTTTGAGAAACCCAAAGAAGATGATTTCAGCTTAGAATTGTCAAAACTTCATACCTATGATGATGTTGTGGAGCGAGTAGCTCAACAACTTGGTTTGGACGATCCATCCAAAATTAGGCTTACCTCACATAATTGTTACTCCCAGCAACCCAAACCTCAGCCAATTAAGTATCGGGGGGTAGACCGTTTGACTGATATGCTTGTTCACTACAATCAGACTTCGGATATATTGTACTATGAAGTATTAGACATCCCTCTGCCTGAATTACAAGGTTTGAAAACCCTGAAAGTTGCATTCCATCATGCTACCAAGGATGAAGTGGTTATTCACACCATAAGACTGCCAAAACAGAGCACTGTTGGGGATGTAATCAATGATCTTAAAACTAAGGTTGAATTGTCTCATCCAGATGCAGAACTTAGGTTGCTCGAGGTTTTCTATCACAAGATATACAAGGTCTTTCCCCAAAGTGAAAAGATCGAAAACATAAATGATCAATACTGGACGTTACGTGCTGAAGAGGTTCCAGAGGAAGAGAAAAATCTTGGTCCTAATGACCGTGTAATTCATGTCTACCACTTCACTAAAGACACTGCTCAGAATCAAATGCAAATTCAAAACTTCGGGGAACCTTTTTTCTTGGTTATACATGAAGGTGAGACCTTGGCTGAAATTAGAGGTCGGATACAAAAGAAATTACAGGTTGCAGATGAGGAATTTGCGAAGTGGAAGTTTGCATTTCTTTCGCTAGGTCGGCCAGAGTATCTTCAGGACACTGACATCGTCTCTAGTCGGTTCCAGAGAAGAGATGTTTATGGTGCTTGGGAGCAGTATCTTGGGCTGGAGCACTCTGACAGCGCTCCTAAAAGAGCTTATGCAGCTAATCAGAATCGCCACACATTTGAGAAGCCGGTGAAAATCTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UNC-93

Protein Analysis

1118

Amino Acids

130.98

Weight (kDa)

5.52

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 56 - 179 3.8e-39 MATH domain
MATH PF00917 61 - 180 1.2e-17 MATH domain
UCH PF00443 199 - 520 1.6e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 199 - 474 2e-15 Ubiquitin carboxyl-terminal hydrolase
USP7_ICP0_bdg PF12436 624 - 876 3.9e-78 ICP0-binding domain of Ubiquitin-specific protease 7
USP7_C2 PF14533 886 - 1096 1.1e-55 Ubiquitin-specific protease C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 521
AatII GACGTC 1 cut(s) 2376
AccB7I CCANNNNNTGG 1 cut(s) 94
AccI GTMKAC 2 cut(s) 2607, 3000
AccII CGCG 1 cut(s) 1544
AciI CCGC 6 cut(s) 314, 515, 530, 1487, 1873, 2362
AclWI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AcoI YGGCCR 1 cut(s) 3182
AcsI RAATTY 6 cut(s) 325, 1409, 1523, 2276, 3039, 3143
AcuI CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
AcyI GRCGYC 1 cut(s) 2373
AfeI AGCGCT 1 cut(s) 3286
AfiI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
AflII CTTAAG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 1542
AjnI CCWGG 1 cut(s) 1498
AjuI GAANNNNNNNTTGG 6 cut(s) 1994, 2007, 2026, 2039, 2319, 2351
AleI CACNNNNGTG 2 cut(s) 1211, 1344
Alw21I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
Alw26I GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
Alw44I GTGCAC 1 cut(s) 2396
AlwI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 2 cut(s) 1716, 2855
Aor51HI AGCGCT 1 cut(s) 3286
AoxI GGCC 2 cut(s) 60, 3182
ApaLI GTGCAC 1 cut(s) 2396
ApeKI GCWGC 2 cut(s) 835, 3305
ApoI RAATTY 6 cut(s) 325, 1409, 1523, 2276, 3039, 3143
AseI ATTAAT 2 cut(s) 963, 1221
Asp700I GAANNNNTTC 2 cut(s) 2006, 2949
AspLEI GCGC 1 cut(s) 3287
AspS9I GGNCC 2 cut(s) 2027, 2975
AsuC2I CCSGG 2 cut(s) 1717, 1718
AsuHPI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
AvaI CYCGRG 2 cut(s) 1716, 2855
AvaII GGWCC 2 cut(s) 2027, 2975
BaeGI GKGCMC 2 cut(s) 1453, 2400
BanII GRGCYC 1 cut(s) 2247
BauI CACGAG 1 cut(s) 503
Bbv12I GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BbvCI CCTCAGC 1 cut(s) 2583
BbvI GCAGC 2 cut(s) 847, 3317
BccI CCATC 7 cut(s) 721, 804, 2273, 2300, 2386, 2539, 2733
BceAI ACGGC 1 cut(s) 2147
BcgI CGANNNNNNTGC 2 cut(s) 3153, 3187
BciT130I CCWGG 1 cut(s) 1500
BciVI GTATCC 2 cut(s) 808, 3105
BclI TGATCA 3 cut(s) 28, 1396, 2920
BcnI CCSGG 2 cut(s) 1717, 1718
BcoDI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BfaI CTAG 3 cut(s) 195, 3176, 3216
BfmI CTRYAG 1 cut(s) 641
BfoI RGCGCY 1 cut(s) 3288
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 2 cut(s) 808, 3105
BisI GCNGC 2 cut(s) 836, 3306
BlsI GCNGC 2 cut(s) 837, 3307
BmcAI AGTACT 1 cut(s) 2149
Bme1390I CCNGG 3 cut(s) 1500, 1717, 1718
Bme18I GGWCC 2 cut(s) 2027, 2975
BmeT110I CYCGRG 2 cut(s) 1716, 2855
BmgT120I GGNCC 2 cut(s) 2027, 2975
BmiI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BmrFI CCNGG 3 cut(s) 1500, 1717, 1718
BmsI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
Bpu10I CCTNAGC 2 cut(s) 1842, 2583
BpuEI CTTGAG 2 cut(s) 1097, 2321
BpuMI CCSGG 2 cut(s) 1717, 1718
Bsa29I ATCGAT 1 cut(s) 2268
BsaAI YACGTR 2 cut(s) 1565, 2939
BsaHI GRCGYC 1 cut(s) 2373
BsaI GGTCTC 1 cut(s) 3080
BsaJI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BsaWI WCCGGW 2 cut(s) 2029, 2338
BsaXI ACNNNNNCTCC 4 cut(s) 1448, 1478, 2301, 2331
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
Bse118I RCCGGY 1 cut(s) 3337
Bse1I ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
Bse3DI GCAATG 2 cut(s) 1600, 1611
BseBI CCWGG 1 cut(s) 1500
BseCI ATCGAT 1 cut(s) 2268
BseDI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BseLI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BseMI GCAATG 2 cut(s) 1600, 1611
BseMII CTCAG 3 cut(s) 690, 2597, 3038
BseNI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BseRI GAGGAG 4 cut(s) 59, 1472, 1847, 2225
BseSI GKGCMC 2 cut(s) 1453, 2400
BseXI GCAGC 2 cut(s) 847, 3317
BseYI CCCAGC 2 cut(s) 488, 2568
Bsh1236I CGCG 1 cut(s) 1544
Bsh1285I CGRYCG 1 cut(s) 2426
BshFI GGCC 2 cut(s) 62, 3184
BshVI ATCGAT 1 cut(s) 2268
BsiEI CGRYCG 1 cut(s) 2426
BsiHKAI GWGCWC 4 cut(s) 1665, 2400, 2783, 3276
BsiHKCI CYCGRG 2 cut(s) 1716, 2855
BsiSI CCGG 5 cut(s) 59, 1717, 2030, 2339, 3338
BslFI GGGAC 2 cut(s) 1733, 2360
BslI CCNNNNNNNGG 7 cut(s) 94, 494, 1828, 2209, 2227, 2302, 2541
BsmAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BsmBI CGTCTC 2 cut(s) 2411, 3217
BsmFI GGGAC 2 cut(s) 1733, 2360
BsmI GAATGC 2 cut(s) 962, 2720
BsnI GGCC 2 cut(s) 62, 3184
Bso31I GGTCTC 1 cut(s) 3080
BsoBI CYCGRG 2 cut(s) 1716, 2855
Bsp1286I GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
Bsp1407I TGTACA 1 cut(s) 1557
Bsp19I CCATGG 2 cut(s) 93, 1209
BspACI CCGC 6 cut(s) 314, 515, 530, 1487, 1873, 2362
BspANI GGCC 2 cut(s) 62, 3184
BspCNI CTCAG 3 cut(s) 691, 2596, 3037
BspDI ATCGAT 1 cut(s) 2268
BspFNI CGCG 1 cut(s) 1544
BspHI TCATGA 2 cut(s) 9, 1774
BspLI GGNNCC 4 cut(s) 913, 2952, 3057, 3224
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 6 cut(s) 146, 294, 479, 1779, 2093, 2522
BspQI GCTCTTC 2 cut(s) 741, 1473
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 3080
BsrDI GCAATG 2 cut(s) 1600, 1611
BsrFI RCCGGY 1 cut(s) 3337
BsrGI TGTACA 1 cut(s) 1557
BsrI ACTGG 8 cut(s) 143, 760, 882, 1458, 1859, 2155, 2333, 2933
BssAI RCCGGY 1 cut(s) 3337
BssECI CCNNGG 9 cut(s) 63, 93, 270, 610, 1209, 1498, 1716, 2736, 3090
BssNI GRCGYC 1 cut(s) 2373
BssSI CACGAG 1 cut(s) 503
BssT1I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Bst2BI CACGAG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 1500
Bst6I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
BstACI GRCGYC 1 cut(s) 2373
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 1557
BstBAI YACGTR 2 cut(s) 1565, 2939
BstC8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
BstDEI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
BstDSI CCRYGG 2 cut(s) 93, 1209
BstEII GGTNACC 1 cut(s) 935
BstENI CCTNNNNNAGG 2 cut(s) 1826, 2225
BstFNI CGCG 1 cut(s) 1544
BstH2I RGCGCY 1 cut(s) 3288
BstHHI GCGC 1 cut(s) 3287
BstMAI GTCTC 6 cut(s) 1197, 2304, 2411, 2832, 3080, 3217
BstMCI CGRYCG 1 cut(s) 2426
BstMWI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
BstNI CCWGG 1 cut(s) 1500
BstNSI RCATGY 2 cut(s) 624, 1552
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 3 cut(s) 1498, 1715, 1716
BstSFI CTRYAG 1 cut(s) 641
BstSLI GKGCMC 2 cut(s) 1453, 2400
BstUI CGCG 1 cut(s) 1544
BstV1I GCAGC 2 cut(s) 847, 3317
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 2268
BsuI GTATCC 2 cut(s) 808, 3105
BsuRI GGCC 2 cut(s) 62, 3184
BsuTUI ATCGAT 1 cut(s) 2268
BtgI CCRYGG 2 cut(s) 93, 1209
BtsI GCAGTG 1 cut(s) 3018
Cac8I GCNNGC 4 cut(s) 2160, 2194, 2293, 2297
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 2 cut(s) 9, 1774
CfoI GCGC 1 cut(s) 3287
Cfr10I RCCGGY 1 cut(s) 3337
Cfr13I GGNCC 2 cut(s) 2027, 2975
Cfr9I CCCGGG 1 cut(s) 1716
ClaI ATCGAT 1 cut(s) 2268
CseI GACGC 2 cut(s) 1396, 1883
DdeI CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
EaeI YGGCCR 1 cut(s) 3182
Eam1104I CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
EarI CTCTTC 7 cut(s) 741, 1145, 1473, 2138, 2940, 2955, 3226
Eco130I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Eco24I GRGCYC 1 cut(s) 2247
Eco31I GGTCTC 1 cut(s) 3080
Eco47I GGWCC 2 cut(s) 2027, 2975
Eco47III AGCGCT 1 cut(s) 3286
Eco57I CTGAAG 6 cut(s) 722, 992, 1308, 2021, 2964, 3179
Eco88I CYCGRG 2 cut(s) 1716, 2855
Eco91I GGTNACC 1 cut(s) 935
EcoNI CCTNNNNNAGG 2 cut(s) 1826, 2225
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 1498
EcoT14I CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
EcoT22I ATGCAT 1 cut(s) 962
EcoT38I GRGCYC 1 cut(s) 2247
ErhI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
Esp3I CGTCTC 2 cut(s) 2411, 3217
FalI AAGNNNNNCTT 2 cut(s) 1994, 2026
FaqI GGGAC 2 cut(s) 1733, 2360
FauNDI CATATG 5 cut(s) 334, 568, 687, 820, 942
FbaI TGATCA 3 cut(s) 28, 1396, 2920
FblI GTMKAC 2 cut(s) 2607, 3000
Fnu4HI GCNGC 2 cut(s) 836, 3306
FriOI GRGCYC 1 cut(s) 2247
Fsp4HI GCNGC 2 cut(s) 836, 3306
FspBI CTAG 3 cut(s) 195, 3176, 3216
GlaI GCGC 1 cut(s) 3286
GluI GCNGC 2 cut(s) 836, 3306
GsaI CCCAGC 2 cut(s) 492, 2572
GsuI CTGGAG 5 cut(s) 743, 899, 1475, 1754, 3290
HaeII RGCGCY 1 cut(s) 3288
HaeIII GGCC 2 cut(s) 62, 3184
HapII CCGG 5 cut(s) 59, 1717, 2030, 2339, 3338
HgaI GACGC 2 cut(s) 1396, 1883
HhaI GCGC 1 cut(s) 3287
Hin1I GRCGYC 1 cut(s) 2373
Hin6I GCGC 1 cut(s) 3285
HinP1I GCGC 1 cut(s) 3285
HindIII AAGCTT 2 cut(s) 212, 2090
HinfI GANTC 9 cut(s) 168, 317, 572, 646, 742, 824, 992, 3028, 3316
HpaII CCGG 5 cut(s) 59, 1717, 2030, 2339, 3338
HphI GGTGA 8 cut(s) 77, 485, 929, 1225, 1275, 1484, 3095, 3352
Hpy99I CGWCG 1 cut(s) 1390
HpyAV CCTTC 9 cut(s) 168, 574, 886, 926, 1016, 1600, 1739, 1741, 3074
HpyCH4IV ACGT 7 cut(s) 1072, 1564, 2119, 2124, 2373, 2933, 2938
HpyF10VI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
HpyF3I CTNAG 8 cut(s) 699, 1125, 1842, 2458, 2583, 2814, 2846, 3024
HpySE526I ACGT 7 cut(s) 1072, 1564, 2119, 2124, 2373, 2933, 2938
Hsp92I GRCGYC 1 cut(s) 2373
HspAI GCGC 1 cut(s) 3285
Ksp22I TGATCA 3 cut(s) 28, 1396, 2920
LguI GCTCTTC 2 cut(s) 741, 1473
LmnI GCTCC 9 cut(s) 614, 1459, 1660, 1735, 2053, 2497, 3253, 3271, 3292
Lsp1109I GCAGC 2 cut(s) 847, 3317
LweI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
MaeI CTAG 3 cut(s) 195, 3176, 3216
MaeII ACGT 7 cut(s) 1072, 1564, 2119, 2124, 2373, 2933, 2938
MaeIII GTNAC 6 cut(s) 935, 1426, 1571, 2120, 2561, 2934
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
MluI ACGCGT 1 cut(s) 1542
MlyI GAGTC 4 cut(s) 566, 640, 751, 1001
MmeI TCCRAC 2 cut(s) 2107, 3089
Mph1103I ATGCAT 1 cut(s) 962
MroXI GAANNNNTTC 2 cut(s) 2006, 2949
MspA1I CMGCKG 2 cut(s) 1873, 2162
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 5 cut(s) 59, 1717, 2030, 2339, 3338
MspR9I CCNGG 3 cut(s) 1500, 1717, 1718
Mva1269I GAATGC 2 cut(s) 962, 2720
MvaI CCWGG 1 cut(s) 1500
MvnI CGCG 1 cut(s) 1544
MwoI GCNNNNNNNGC 6 cut(s) 844, 1870, 1879, 2198, 3181, 3305
NciI CCSGG 2 cut(s) 1717, 1718
NcoI CCATGG 2 cut(s) 93, 1209
NdeI CATATG 5 cut(s) 334, 568, 687, 820, 942
NlaIV GGNNCC 4 cut(s) 913, 2952, 3057, 3224
NmuCI GTSAC 2 cut(s) 935, 1571
NsiI ATGCAT 1 cut(s) 962
NspI RCATGY 2 cut(s) 624, 1552
OliI CACNNNNGTG 2 cut(s) 1211, 1344
PaeR7I CTCGAG 1 cut(s) 2855
PagI TCATGA 2 cut(s) 9, 1774
PciSI GCTCTTC 2 cut(s) 741, 1473
PcsI WCGNNNNNNNCGW 3 cut(s) 1918, 2214, 2223
PctI GAATGC 2 cut(s) 962, 2720
PdmI GAANNNNTTC 2 cut(s) 2006, 2949
PfeI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
PflFI GACNNNGTC 3 cut(s) 799, 1781, 3209
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 2 cut(s) 837, 3307
Ple19I CGATCG 1 cut(s) 2426
PleI GAGTC 4 cut(s) 566, 640, 750, 1000
PpsI GAGTC 4 cut(s) 566, 640, 750, 1000
Ppu21I YACGTR 2 cut(s) 1565, 2939
PshBI ATTAAT 2 cut(s) 963, 1221
PsiI TTATAA 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 1498
PspEI GGTNACC 1 cut(s) 935
PspFI CCCAGC 2 cut(s) 488, 2568
PspGI CCWGG 1 cut(s) 1498
PspN4I GGNNCC 4 cut(s) 913, 2952, 3057, 3224
PspPI GGNCC 2 cut(s) 2027, 2975
PspXI VCTCGAGB 1 cut(s) 2855
PstI CTGCAG 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 3 cut(s) 799, 1781, 3209
PvuI CGATCG 1 cut(s) 2426
PvuII CAGCTG 1 cut(s) 2162
SapI GCTCTTC 2 cut(s) 741, 1473
SatI GCNGC 2 cut(s) 836, 3306
Sau96I GGNCC 2 cut(s) 2027, 2975
ScaI AGTACT 1 cut(s) 2149
SchI GAGTC 4 cut(s) 566, 640, 751, 1001
ScrFI CCNGG 3 cut(s) 1500, 1717, 1718
SduI GDGCHC 6 cut(s) 1453, 1665, 2247, 2400, 2783, 3276
SfaNI GCATC 5 cut(s) 39, 502, 1111, 1800, 2827
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 2855
SinI GGWCC 2 cut(s) 2027, 2975
SlaI CTCGAG 1 cut(s) 2855
SmaI CCCGGG 1 cut(s) 1718
SmlI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SmoI CTYRAG 4 cut(s) 602, 1076, 2300, 2855
SsiI CCGC 6 cut(s) 314, 515, 530, 1487, 1873, 2362
SspI AATATT 1 cut(s) 1277
SspMI CTAG 3 cut(s) 195, 3176, 3216
StyD4I CCNGG 3 cut(s) 1498, 1715, 1716
StyI CCWWGG 6 cut(s) 93, 270, 610, 1209, 2736, 3090
TaiI ACGT 7 cut(s) 1075, 1567, 2122, 2127, 2376, 2936, 2941
TaqII GACCGA 1 cut(s) 1399
TatI WGTACW 4 cut(s) 762, 1557, 2147, 2658
TfiI GAWTC 5 cut(s) 168, 317, 824, 3028, 3316
TseFI GTSAC 2 cut(s) 935, 1571
TseI GCWGC 2 cut(s) 835, 3305
Tsp45I GTSAC 2 cut(s) 935, 1571
TspMI CCCGGG 1 cut(s) 1716
Tth111I GACNNNGTC 3 cut(s) 799, 1781, 3209
Van91I CCANNNNNTGG 1 cut(s) 94
Vha464I CTTAAG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 2396
VpaK11BI GGWCC 2 cut(s) 2027, 2975
VspI ATTAAT 2 cut(s) 963, 1221
XagI CCTNNNNNAGG 2 cut(s) 1826, 2225
XapI RAATTY 6 cut(s) 325, 1409, 1523, 2276, 3039, 3143
XceI RCATGY 2 cut(s) 624, 1552
XcmI CCANNNNNNNNNTGG 2 cut(s) 2303, 2743
XhoI CTCGAG 1 cut(s) 2855
XmaI CCCGGG 1 cut(s) 1716
XmiI GTMKAC 2 cut(s) 2607, 3000
XmnI GAANNNNTTC 2 cut(s) 2006, 2949
XspI CTAG 3 cut(s) 195, 3176, 3216
ZraI GACGTC 1 cut(s) 2374
ZrmI AGTACT 1 cut(s) 2149
Zsp2I ATGCAT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.