Rorug06G0488700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008105
Physical Location & Seq
Reverse (-)
60699654 .. 60700211
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0488700.1

Sequence Viewer

Length: 558 bp
ATGACTACTACTACTATTCACAGTCGCAGCAATAGCAAGCACCAGTTCTACTCCAGAGGCACTGTGCCCTTCTCTTGGGAAAACAAGCCCGGAGTCTCCAAGCTGTCACACATCAAAACCGAAGCGAAAACGGAGTACTACTTCATCAAAGACGACGATTACAAACTACCACCACCGCCTAGGGTTTCTTTCCACGGGGACTACCAGCTCCCTCTTCCTCCTCCCTGCACTTTCCAGGCGCCCATTTCTAGAAACTCCTCCATTAAGGGCTTGATCAGCAACAAAGCCGAAACCGAAGACCCTTTTCAAATGGCCATCCTCGAGTGCACCAAGTCGATCCACAATGGTGGTGGCAATGATAGTGGGGATGTTGCTACCAAAGGTAGGAGGAAAACGTCGTTGTCTTTGTCCCCGAAACAGTACGGTGGGTTTGGATCTGGACTGAGAAGTTTGTTCGGGTCCTTATCGTGTAAATGCTCCAGCAGTGTAAGGGATGAAAGTTTGGTTAAGGTCTCTCGGGTTCCATACCAGAGAGATACAGTACTTATTAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.51

Weight (kDa)

9.45

Isoelectric Point (pI)

62.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AciI CCGC 1 cut(s) 176
AclWI GGATC 2 cut(s) 331, 442
AcoI YGGCCR 1 cut(s) 312
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 3 cut(s) 137, 422, 543
AfiI CCNNNNNNNGG 2 cut(s) 75, 384
AgsI TTSAA 1 cut(s) 308
AjnI CCWGG 1 cut(s) 234
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 2 cut(s) 103, 208
AluI AGCT 2 cut(s) 103, 208
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 100, 517
Alw44I GTGCAC 1 cut(s) 325
AlwI GGATC 2 cut(s) 331, 442
Ama87I CYCGRG 2 cut(s) 320, 516
AoxI GGCC 1 cut(s) 312
ApaLI GTGCAC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 27
AseI ATTAAT 1 cut(s) 549
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 459
AsuC2I CCSGG 1 cut(s) 90
AvaI CYCGRG 2 cut(s) 320, 516
AvaII GGWCC 1 cut(s) 459
AvrII CCTAGG 1 cut(s) 179
BaeGI GKGCMC 2 cut(s) 69, 329
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 39
BccI CCATC 1 cut(s) 323
BciT130I CCWGG 1 cut(s) 236
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 100, 517
BfaI CTAG 2 cut(s) 180, 249
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 1 cut(s) 28
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 1 cut(s) 29
BmcAI AGTACT 2 cut(s) 137, 543
Bme1390I CCNGG 2 cut(s) 90, 236
Bme18I GGWCC 1 cut(s) 459
BmeT110I CYCGRG 2 cut(s) 320, 516
BmgT120I GGNCC 1 cut(s) 459
BmiI GGNNCC 3 cut(s) 240, 460, 522
BmrFI CCNGG 2 cut(s) 90, 236
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 2 cut(s) 37, 463
BpuMI CCSGG 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 517
BsaJI CCNNGG 2 cut(s) 179, 193
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 384
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 361
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 179, 193
BseGI GGATG 3 cut(s) 315, 373, 499
BseLI CCNNNNNNNGG 2 cut(s) 75, 384
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 2 cut(s) 210, 247
BseSI GKGCMC 2 cut(s) 69, 329
BseXI GCAGC 1 cut(s) 39
BsgI GTGCAG 1 cut(s) 211
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 2 cut(s) 320, 516
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 212, 394
BslI CCNNNNNNNGG 2 cut(s) 75, 384
BsmAI GTCTC 2 cut(s) 100, 517
BsmFI GGGAC 2 cut(s) 212, 394
BsnI GGCC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 517
BsoBI CYCGRG 2 cut(s) 320, 516
Bsp1286I GDGCHC 2 cut(s) 69, 329
Bsp143I GATC 3 cut(s) 273, 336, 434
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 240, 460, 522
BspPI GGATC 2 cut(s) 331, 442
BspT107I GGYRCC 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 361
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 179, 193
BssMI GATC 3 cut(s) 273, 336, 434
BssNI GRCGYC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 5 cut(s) 23, 64, 420, 425, 541
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 3 cut(s) 315, 373, 499
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 276, 339, 437
BstMAI GTCTC 2 cut(s) 100, 517
BstMBI GATC 3 cut(s) 273, 336, 434
BstMWI GCNNNNNNNGC 2 cut(s) 33, 276
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 88, 234
BstSLI GKGCMC 2 cut(s) 69, 329
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 3 cut(s) 315, 373, 499
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 2 cut(s) 60, 490
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 459
Csp6I GTAC 3 cut(s) 136, 421, 542
CspCI CAANNNNNGTGG 4 cut(s) 331, 343, 366, 378
CviJI RGCY 6 cut(s) 88, 103, 208, 270, 287, 314
CviKI_1 RGCY 6 cut(s) 88, 103, 208, 270, 287, 314
CviQI GTAC 3 cut(s) 136, 421, 542
DdeI CTNAG 1 cut(s) 443
DinI GGCGCC 1 cut(s) 240
DpnI GATC 3 cut(s) 275, 338, 436
DpnII GATC 3 cut(s) 273, 336, 434
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 179
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 320, 516
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 179
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 179
FaiI YATR 1 cut(s) 526
FaqI GGGAC 2 cut(s) 212, 394
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 3 cut(s) 302, 380, 506
Fsp4HI GCNGC 1 cut(s) 28
FspBI CTAG 2 cut(s) 180, 249
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 28
GsuI CTGGAG 2 cut(s) 37, 463
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 1 cut(s) 314
HapII CCGG 1 cut(s) 90
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 239
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 1 cut(s) 93
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 1 cut(s) 327
Hpy188III TCNNGA 3 cut(s) 54, 249, 438
Hpy8I GTNNAC 1 cut(s) 327
Hpy99I CGWCG 2 cut(s) 158, 400
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 5 cut(s) 23, 64, 420, 425, 541
HpyCH4IV ACGT 1 cut(s) 395
HpyCH4V TGCA 2 cut(s) 228, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 276
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 1 cut(s) 395
Hsp92I GRCGYC 1 cut(s) 239
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 3 cut(s) 273, 336, 434
LmnI GCTCC 2 cut(s) 213, 482
Lsp1109I GCAGC 1 cut(s) 39
MaeI CTAG 2 cut(s) 180, 249
MaeII ACGT 1 cut(s) 395
MaeIII GTNAC 1 cut(s) 105
MalI GATC 3 cut(s) 275, 338, 436
MboI GATC 3 cut(s) 273, 336, 434
MboII GAAGA 2 cut(s) 206, 308
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 2 cut(s) 69, 329
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 1 cut(s) 550
MluNI TGGCCA 1 cut(s) 314
Mly113I GGCGCC 1 cut(s) 239
MlyI GAGTC 1 cut(s) 102
MnlI CCTC 7 cut(s) 50, 222, 228, 231, 268, 329, 381
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 4 cut(s) 264, 507, 549, 556
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 90, 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 33, 276
NarI GGCGCC 1 cut(s) 239
NciI CCSGG 1 cut(s) 90
NdeII GATC 3 cut(s) 273, 336, 434
NlaIV GGNNCC 3 cut(s) 240, 460, 522
NmuCI GTSAC 1 cut(s) 105
OliI CACNNNNGTG 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 320
PkrI GCNGC 1 cut(s) 29
PleI GAGTC 1 cut(s) 101
PluTI GGCGCC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 240, 460, 522
PspPI GGNCC 1 cut(s) 459
PspPPI RGGWCCY 1 cut(s) 459
PspXI VCTCGAGB 1 cut(s) 320
PsuI RGATCY 1 cut(s) 434
RsaI GTAC 3 cut(s) 137, 422, 543
RsaNI GTAC 3 cut(s) 136, 421, 542
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 4 cut(s) 264, 507, 549, 556
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 3 cut(s) 273, 336, 434
Sau96I GGNCC 1 cut(s) 459
ScaI AGTACT 2 cut(s) 137, 543
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 2 cut(s) 90, 236
SduI GDGCHC 2 cut(s) 69, 329
SetI ASST 5 cut(s) 105, 210, 385, 398, 513
SfoI GGCGCC 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 1 cut(s) 459
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 550
SsiI CCGC 1 cut(s) 176
SspDI GGCGCC 1 cut(s) 238
SspMI CTAG 2 cut(s) 180, 249
StyD4I CCNGG 2 cut(s) 88, 234
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 5 cut(s) 23, 64, 420, 425, 541
TaiI ACGT 1 cut(s) 398
TaqI TCGA 2 cut(s) 321, 335
TasI AATT 1 cut(s) 550
TatI WGTACW 2 cut(s) 135, 541
Tru1I TTAA 4 cut(s) 264, 507, 549, 556
Tru9I TTAA 4 cut(s) 264, 507, 549, 556
TscAI CASTG 2 cut(s) 67, 490
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 2 cut(s) 133, 510
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 2 cut(s) 67, 490
VneI GTGCAC 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 459
VspI ATTAAT 1 cut(s) 549
XbaI TCTAGA 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XhoI CTCGAG 1 cut(s) 320
XmaJI CCTAGG 1 cut(s) 179
XspI CTAG 2 cut(s) 180, 249
ZrmI AGTACT 2 cut(s) 137, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.