Rh2AG262400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007239
Physical Location & Seq
Forward (+)
30297116 .. 30299542
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG262400.1

Sequence Viewer

Length: 921 bp
ATGGGTGATATAGAAGAGGCGAGATTGATTTTTGATGGTTTAAAAGTTAAAAGTGCTGTTACTTGGACTATTGTTATAGCAGGGTATGCGAAAAGTGGAAGAAGTGAAGCGGCTTTGAAGTCGTTTTATCATATGAAAGACACTGATGTTCTTCCTGATAAATATGTGCTCTCTGCTCTTCTGACCGCATGTTCTGCCCTCAAGTTCATTGGAGGCGGCAAGCAAATTCATGCTCATGTGCTGAGAAGGGGAACAGAAATGGATGTTTCAGTGGTTAATGTACTAATAGATTTCTACACAAAGTGCGGTCAAGTCCAGGCAGGACATAAACTATTTGATAAGATGGTAGATAAGGACCTTATTTCATGGACCACAATGATAGCAGGGTACATGCAAAACTCGATGCACATGGAGGCCGTTAAGCTATTTTCTGAAATGACCAGAATGGGTTGGAAGGCAGATGGATATGGGTGCAGTAGCATTCTTACTTCATGTGGTTCACTTGAGGCTCTGAAACATGGGAGAGAAGTGCACGCTTATACTATCAGGGTTGATCTTGTCTACGAATATTATGTGAAGAATAGTTTGATTGATATGTATGCAAAATGTGATTCCCTGACTGATGCAAGAAGAGTTTTTGATTCCATGGCTGATCATAATGTGGTCTCTTACAATGCAATGATTGAAGGATACTCAAGGCAGGATAAGGTGGCTGAAGCTCTGGATCTTTTCAATCTTATGAGGCTTAAATTGGTCCAGCCTAGTATCTTGACATTTGTCAGTCTTCTTGGTGTCTCAGCTGCTTTATTGACCTTGGAATTAAGCAAGCAAATCCATGGGCTGACCACCAAGTACGGAGAACATGAGACTGTACGATGGGAAAAACATTGGAAAAACCTTATATTGGTAAAATTAGGATGA

Protein Analysis

306

Amino Acids

34.36

Weight (kDa)

8.15

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 18 - 64 3.7e-09 PPR repeat family
PPR_2 PF13041 119 - 165 8.8e-09 PPR repeat family
PPR PF01535 121 - 150 2.4e-07 PPR repeat
PPR_2 PF13041 220 - 263 1.2e-11 PPR repeat family
PPR_1 PF12854 220 - 248 4e-06 PPR repeat
PPR_3 PF13812 220 - 265 4.1e-06 Pentatricopeptide repeat domain
PPR PF01535 222 - 248 5.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 561
AciI CCGC 4 cut(s) 110, 186, 216, 306
AclWI GGATC 1 cut(s) 732
AcsI RAATTY 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 735
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 4 cut(s) 282, 389, 854, 873
AfiI CCNNNNNNNGG 1 cut(s) 904
AgsI TTSAA 3 cut(s) 118, 686, 733
AjnI CCWGG 1 cut(s) 315
AluBI AGCT 3 cut(s) 424, 719, 800
AluI AGCT 3 cut(s) 424, 719, 800
Alw21I GWGCWC 2 cut(s) 171, 534
Alw26I GTCTC 3 cut(s) 670, 799, 860
Alw44I GTGCAC 1 cut(s) 530
AlwI GGATC 1 cut(s) 732
AoxI GGCC 1 cut(s) 414
ApaLI GTGCAC 1 cut(s) 530
ApeKI GCWGC 1 cut(s) 800
ApoI RAATTY 1 cut(s) 225
AspS9I GGNCC 3 cut(s) 355, 369, 754
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 3 cut(s) 355, 369, 754
BaeGI GKGCMC 1 cut(s) 534
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 2 cut(s) 171, 534
BbvI GCAGC 1 cut(s) 787
BccI CCATC 4 cut(s) 29, 337, 455, 870
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 317
BciVI GTATCC 1 cut(s) 683
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 670, 799, 860
BfaI CTAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 3 cut(s) 111, 217, 801
BlsI GCNGC 3 cut(s) 112, 218, 802
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 3 cut(s) 355, 369, 754
BmgT120I GGNCC 3 cut(s) 355, 369, 754
BmrFI CCNGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 393, 613
BoxI GACNNNNGTC 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 776
BpuEI CTTGAG 3 cut(s) 185, 524, 679
BsaI GGTCTC 1 cut(s) 670
BsaJI CCNNGG 3 cut(s) 645, 813, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 904
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 3 cut(s) 645, 813, 835
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 1 cut(s) 904
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 233, 810
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 1 cut(s) 787
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 1 cut(s) 416
BsiHKAI GWGCWC 2 cut(s) 171, 534
BslI CCNNNNNNNGG 1 cut(s) 904
BsmAI GTCTC 3 cut(s) 670, 799, 860
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 171, 534
Bsp143I GATC 3 cut(s) 553, 652, 724
Bsp19I CCATGG 2 cut(s) 645, 835
BspACI CCGC 4 cut(s) 110, 186, 216, 306
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 2 cut(s) 234, 809
BspPI GGATC 1 cut(s) 732
BspQI GCTCTTC 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 670
BsrDI GCAATG 1 cut(s) 684
BssECI CCNNGG 3 cut(s) 645, 813, 835
BssMI GATC 3 cut(s) 553, 652, 724
BssT1I CCWWGG 3 cut(s) 645, 813, 835
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 871
Bst6I CTCTTC 3 cut(s) 9, 183, 625
BstAPI GCANNNNNTGC 2 cut(s) 86, 194
BstC8I GCNNGC 3 cut(s) 221, 534, 827
BstDEI CTNAG 2 cut(s) 242, 796
BstDSI CCRYGG 2 cut(s) 645, 835
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 3 cut(s) 556, 655, 727
BstMAI GTCTC 3 cut(s) 670, 799, 860
BstMBI GATC 3 cut(s) 553, 652, 724
BstMWI GCNNNNNNNGC 2 cut(s) 86, 194
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 2 cut(s) 192, 394
BstPAI GACNNNNGTC 1 cut(s) 776
BstSCI CCNGG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 534
BstV1I GCAGC 1 cut(s) 787
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 1 cut(s) 724
BstYI RGATCY 1 cut(s) 724
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 1 cut(s) 416
BtgI CCRYGG 2 cut(s) 645, 835
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 141, 276
Cac8I GCNNGC 3 cut(s) 221, 534, 827
Cfr13I GGNCC 3 cut(s) 355, 369, 754
Csp6I GTAC 4 cut(s) 281, 388, 853, 872
CviQI GTAC 4 cut(s) 281, 388, 853, 872
DdeI CTNAG 2 cut(s) 242, 796
DpnI GATC 3 cut(s) 555, 654, 726
DpnII GATC 3 cut(s) 553, 652, 724
DraI TTTAAA 1 cut(s) 42
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 3 cut(s) 9, 183, 625
EarI CTCTTC 3 cut(s) 9, 183, 625
Eco130I CCWWGG 3 cut(s) 645, 813, 835
Eco31I GGTCTC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 355, 369, 754
Eco57I CTGAAG 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 315
EcoT14I CCWWGG 3 cut(s) 645, 813, 835
ErhI CCWWGG 3 cut(s) 645, 813, 835
FauNDI CATATG 1 cut(s) 132
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 111, 217, 801
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 3 cut(s) 111, 217, 801
FspBI CTAG 1 cut(s) 762
GluI GCNGC 3 cut(s) 111, 217, 801
HaeIII GGCC 1 cut(s) 416
HinfI GANTC 2 cut(s) 611, 641
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 3 cut(s) 500, 532, 562
Hpy188I TCNGA 3 cut(s) 183, 433, 513
Hpy188III TCNNGA 3 cut(s) 155, 722, 769
Hpy8I GTNNAC 3 cut(s) 500, 532, 562
HpyAV CCTTC 3 cut(s) 240, 448, 680
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4V TGCA 7 cut(s) 394, 406, 474, 532, 602, 626, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 194
HpyF3I CTNAG 2 cut(s) 242, 796
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 3 cut(s) 553, 652, 724
LguI GCTCTTC 1 cut(s) 183
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 2 cut(s) 393, 613
MaeI CTAG 1 cut(s) 762
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 555, 654, 726
MboI GATC 3 cut(s) 553, 652, 724
MboII GAAGA 7 cut(s) 26, 111, 143, 170, 589, 642, 776
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 2 cut(s) 171, 534
MluCI AATT 4 cut(s) 225, 749, 818, 911
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 6 cut(s) 10, 206, 209, 406, 499, 735
MseI TTAA 6 cut(s) 41, 48, 276, 420, 747, 821
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 800
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 480
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 86, 194
NcoI CCATGG 2 cut(s) 645, 835
NdeI CATATG 1 cut(s) 132
NdeII GATC 3 cut(s) 553, 652, 724
NspI RCATGY 2 cut(s) 192, 394
PciSI GCTCTTC 1 cut(s) 183
PctI GAATGC 1 cut(s) 480
PfeI GAWTC 2 cut(s) 611, 641
PkrI GCNGC 3 cut(s) 112, 218, 802
PpuMI RGGWCCY 1 cut(s) 355
PshAI GACNNNNGTC 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 315
PspGI CCWGG 1 cut(s) 315
PspPI GGNCC 3 cut(s) 355, 369, 754
PspPPI RGGWCCY 1 cut(s) 355
PsuI RGATCY 1 cut(s) 724
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 4 cut(s) 282, 389, 854, 873
RsaNI GTAC 4 cut(s) 281, 388, 853, 872
RseI CAYNNNNRTG 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 6 cut(s) 41, 48, 276, 420, 747, 821
SatI GCNGC 3 cut(s) 111, 217, 801
Sau3AI GATC 3 cut(s) 553, 652, 724
Sau96I GGNCC 3 cut(s) 355, 369, 754
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 2 cut(s) 171, 534
SetI ASST 7 cut(s) 360, 426, 711, 721, 802, 815, 900
SfaNI GCATC 2 cut(s) 393, 613
SinI GGWCC 3 cut(s) 355, 369, 754
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 200, 503, 694
SmoI CTYRAG 3 cut(s) 200, 503, 694
Sse9I AATT 4 cut(s) 225, 749, 818, 911
SsiI CCGC 4 cut(s) 110, 186, 216, 306
SspI AATATT 1 cut(s) 569
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 3 cut(s) 645, 813, 835
TaaI ACNGT 1 cut(s) 871
TaqI TCGA 1 cut(s) 401
TasI AATT 4 cut(s) 225, 749, 818, 911
TatI WGTACW 1 cut(s) 280
TauI GCSGC 2 cut(s) 113, 219
TfiI GAWTC 2 cut(s) 611, 641
Tru1I TTAA 6 cut(s) 41, 48, 276, 420, 747, 821
Tru9I TTAA 6 cut(s) 41, 48, 276, 420, 747, 821
TscAI CASTG 2 cut(s) 148, 276
TseI GCWGC 1 cut(s) 800
TspDTI ATGAA 5 cut(s) 149, 196, 218, 354, 480
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 2 cut(s) 148, 276
VneI GTGCAC 1 cut(s) 530
VpaK11BI GGWCC 3 cut(s) 355, 369, 754
XapI RAATTY 1 cut(s) 225
XceI RCATGY 2 cut(s) 192, 394
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.