Rh3DG071600

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006425
Physical Location & Seq
Reverse (-)
4871125 .. 4872423
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG071600.1

Sequence Viewer

Length: 1299 bp
ATGGATGATATTGTCAATACCTTTGATGATGCGGGTGTTGTTATTGGAAATATAAAATTCGAGATTCTTAAAGGTGATCTAGCAAAGTTATGGTTCAAGAAGCTGAGTTGTGCCAAAAAAAAGCAGGACAAGATAATTCAACTGGTGGACATAAAGAAAGGGTTGTCTAAGTTGTTTTTCGTGAAAGACATTGAGGAAATTGAGAAGGTCAAGAAAGCCGCCTACTTTTCTACTGATGTGATGAAGAAGTTTATTACGCCGGAGCTTGACAAGATTATGAAAAGGGAGGAGAAGGTCTCTCATTCTTCTCTAAGTAAGGAGACGTTGAAGGCTATACTGCAACCCTCTAAGACTCATCTGAACCATGCAGATGAATTTTCTATCGCTTTTGATCCAGTTTTCCAAAGTGGGGGGCGTTTTGATCTCAAGCCTGGTGTACCTTGCGATAACAAGCTCCTTAAATATAGTTCTCATGATGTTATCATATCCTCTGTTGGAACCAGGTTTGAGAATTACTGCTCTTTTATTGCCAGGACCTTTCTGATTGATCCCATTGACCTTCAGCGCAAGGCATATGAAGTTCTTTACAAGGCCCATGAAGCAGCCATCAGTGAGTTGAAGCCCGGCAACAAAGTTAGTGCTGCTTATGAAGCAGCAGTTTCTGTTGTAAAGAAGGAGGGTCCTGAATTGTCTCGTAAGCTGACAAAACATGCTGGATCAGGCATTGGTCTTGAGCTCTCTGAATCTGGGTTGAATCTTAATGAGAAAAATGGGGAACTGGTGAAAGAAGGCATGGTGTTCAATGTGTCCCTCGGATTTCAGAACCTGCAGAGTAATCTAAAAGGAAAAGAATTCTCTCTGTGGCTAGCAGACACTGTTATTGTAAACAGTGTTAATCCTGAGGTTCTGACAGCGGGGTGTTCTAAAACCATTGAGAATATTAAATTACCCTTAGCTGGAGGAGAAAGGCAGCCAGAGTTCGTGATATTAGAAGATGATGATGATGAAGAAATCTGCAAAAAACAGAAAAGAAGAGAGATGGAGGACTTGGAAGAAGCTTTTAGTATACTGACCCAATTGGATGCAAATATTAATGATACTGAGGCGCTTAATACCAAGTTGGAAGAGATCAATGCTGCAAGAGAAAAAAATAAAAGTGAGGATCAGGAAGACTTGGCAGCTTTACATGTGCATGAGAAAAAGATAAGCGACAGATTGAAGATGATGGAGTTGGCTAGGAAAGCTCAATCAGAATTTGACGAATGTAATTTCCTATTTGACGAATTTGATCACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

432

Amino Acids

48.7

Weight (kDa)

5.46

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 69 - 296 1.7e-29 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 834
AccI GTMKAC 1 cut(s) 1066
AciI CCGC 3 cut(s) 32, 219, 914
AclWI GGATC 4 cut(s) 386, 542, 724, 1170
AcsI RAATTY 5 cut(s) 56, 374, 851, 1253, 1283
AcuI CTGAAG 1 cut(s) 545
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 2 cut(s) 409, 956
AflIII ACRYGT 1 cut(s) 1186
AgsI TTSAA 7 cut(s) 97, 140, 328, 619, 754, 802, 1219
AjnI CCWGG 3 cut(s) 430, 500, 530
AluBI AGCT 9 cut(s) 103, 265, 454, 700, 736, 956, 1058, 1181, 1244
AluI AGCT 9 cut(s) 103, 265, 454, 700, 736, 956, 1058, 1181, 1244
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 3 cut(s) 301, 314, 696
AlwI GGATC 4 cut(s) 386, 542, 724, 1170
AlwNI CAGNNNCTG 3 cut(s) 662, 826, 875
AoxI GGCC 1 cut(s) 591
ApeKI GCWGC 6 cut(s) 602, 641, 653, 970, 1136, 1178
ApoI RAATTY 5 cut(s) 56, 374, 851, 1253, 1283
AseI ATTAAT 1 cut(s) 1092
AspLEI GCGC 2 cut(s) 567, 1108
AspS9I GGNCC 3 cut(s) 534, 592, 680
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 3 cut(s) 86, 793, 1283
AsuNHI GCTAGC 1 cut(s) 865
AvaII GGWCC 2 cut(s) 534, 680
AxyI CCTNAGG 1 cut(s) 900
BanII GRGCYC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 1176
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 6 cut(s) 614, 628, 665, 982, 1123, 1190
BccI CCATC 3 cut(s) 614, 1033, 1219
BciT130I CCWGG 3 cut(s) 432, 502, 532
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 3 cut(s) 301, 314, 696
BfaI CTAG 3 cut(s) 80, 866, 1236
BfmI CTRYAG 1 cut(s) 827
BfoI RGCGCY 1 cut(s) 1109
BfuAI ACCTGC 1 cut(s) 834
BisI GCNGC 7 cut(s) 219, 603, 642, 654, 971, 1137, 1179
BlsI GCNGC 7 cut(s) 220, 604, 643, 655, 972, 1138, 1180
Bme1390I CCNGG 4 cut(s) 432, 502, 532, 624
Bme18I GGWCC 2 cut(s) 534, 680
BmgT120I GGNCC 3 cut(s) 534, 592, 680
BmiI GGNNCC 2 cut(s) 499, 681
BmrFI CCNGG 4 cut(s) 432, 502, 532, 624
BmsI GCATC 2 cut(s) 19, 1072
BmtI GCTAGC 1 cut(s) 869
BpiI GAAGAC 1 cut(s) 1176
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 1 cut(s) 978
Bpu10I CCTNAGC 1 cut(s) 952
BpuEI CTTGAG 2 cut(s) 410, 752
BpuMI CCSGG 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 811
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 956
Bse1I ACTGG 3 cut(s) 147, 395, 783
Bse21I CCTNAGG 1 cut(s) 900
BseBI CCWGG 3 cut(s) 432, 502, 532
BseDI CCNNGG 1 cut(s) 811
BseGI GGATG 2 cut(s) 10, 1087
BseLI CCNNNNNNNGG 2 cut(s) 409, 956
BseMII CTCAG 3 cut(s) 95, 891, 1092
BseNI ACTGG 3 cut(s) 147, 395, 783
BseRI GAGGAG 2 cut(s) 302, 975
BseXI GCAGC 6 cut(s) 614, 628, 665, 982, 1123, 1190
BshFI GGCC 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 2 cut(s) 260, 624
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 2 cut(s) 409, 956
BsmAI GTCTC 3 cut(s) 301, 314, 696
BsmBI CGTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 1 cut(s) 593
Bso31I GGTCTC 1 cut(s) 301
Bsp1286I GDGCHC 1 cut(s) 738
Bsp143I GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
BspACI CCGC 3 cut(s) 32, 219, 914
BspANI GGCC 1 cut(s) 593
BspCNI CTCAG 3 cut(s) 96, 892, 1093
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 2 cut(s) 499, 681
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 1 cut(s) 834
BspOI GCTAGC 1 cut(s) 869
BspPI GGATC 4 cut(s) 386, 542, 724, 1170
BspTNI GGTCTC 1 cut(s) 301
BsrI ACTGG 3 cut(s) 147, 395, 783
BssECI CCNNGG 1 cut(s) 811
BssMI GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
BssNAI GTATAC 1 cut(s) 1067
Bst1107I GTATAC 1 cut(s) 1067
Bst2UI CCWGG 3 cut(s) 432, 502, 532
Bst4CI ACNGT 2 cut(s) 877, 890
Bst6I CTCTTC 2 cut(s) 1027, 1119
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 7 cut(s) 104, 168, 311, 348, 900, 952, 1101
BstF5I GGATG 2 cut(s) 10, 1087
BstH2I RGCGCY 1 cut(s) 1109
BstHHI GCGC 2 cut(s) 567, 1108
BstKTI GATC 8 cut(s) 79, 394, 424, 550, 719, 1131, 1165, 1291
BstMAI GTCTC 3 cut(s) 301, 314, 696
BstMBI GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
BstMWI GCNNNNNNNGC 3 cut(s) 599, 650, 1241
BstNI CCWGG 3 cut(s) 432, 502, 532
BstNSI RCATGY 2 cut(s) 713, 1190
BstSCI CCNGG 4 cut(s) 430, 500, 530, 622
BstSFI CTRYAG 1 cut(s) 827
BstV1I GCAGC 6 cut(s) 614, 628, 665, 982, 1123, 1190
BstV2I GAAGAC 1 cut(s) 1176
BstZ17I GTATAC 1 cut(s) 1067
Bsu36I CCTNAGG 1 cut(s) 900
BsuRI GGCC 1 cut(s) 593
BtsCI GGATG 2 cut(s) 10, 1087
BtsIMutI CAGTG 3 cut(s) 616, 873, 895
BveI ACCTGC 1 cut(s) 834
Cac8I GCNNGC 1 cut(s) 867
CaiI CAGNNNCTG 3 cut(s) 662, 826, 875
CciI TCATGA 1 cut(s) 472
CfoI GCGC 2 cut(s) 567, 1108
Cfr13I GGNCC 3 cut(s) 534, 592, 680
CsiI ACCWGGT 1 cut(s) 500
Csp6I GTAC 1 cut(s) 437
CviAII CATG 7 cut(s) 365, 473, 596, 710, 793, 1187, 1193
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 7 cut(s) 104, 168, 311, 348, 900, 952, 1101
DpnI GATC 8 cut(s) 78, 393, 423, 549, 718, 1130, 1164, 1290
DpnII GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
Eam1104I CTCTTC 2 cut(s) 1027, 1119
EarI CTCTTC 2 cut(s) 1027, 1119
Ecl136II GAGCTC 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 738
Eco31I GGTCTC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 534, 680
Eco53kI GAGCTC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 545
Eco81I CCTNAGG 1 cut(s) 900
EcoICRI GAGCTC 1 cut(s) 736
EcoO109I RGGNCCY 2 cut(s) 534, 680
EcoRI GAATTC 1 cut(s) 851
EcoRII CCWGG 3 cut(s) 430, 500, 530
EcoT38I GRGCYC 1 cut(s) 738
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 7 cut(s) 368, 476, 599, 713, 796, 1190, 1196
FaqI GGGAC 1 cut(s) 793
FatI CATG 7 cut(s) 364, 472, 595, 709, 792, 1186, 1192
FauI CCCGC 2 cut(s) 25, 907
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 1 cut(s) 1288
FblI GTMKAC 1 cut(s) 1066
Fnu4HI GCNGC 7 cut(s) 219, 603, 642, 654, 971, 1137, 1179
FokI GGATG 2 cut(s) 17, 1094
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 7 cut(s) 219, 603, 642, 654, 971, 1137, 1179
FspBI CTAG 3 cut(s) 80, 866, 1236
GlaI GCGC 2 cut(s) 566, 1107
GluI GCNGC 7 cut(s) 219, 603, 642, 654, 971, 1137, 1179
GsuI CTGGAG 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 1109
HaeIII GGCC 1 cut(s) 593
HapII CCGG 2 cut(s) 260, 624
HhaI GCGC 2 cut(s) 567, 1108
Hin1II CATG 7 cut(s) 368, 476, 599, 713, 796, 1190, 1196
Hin6I GCGC 2 cut(s) 565, 1106
HinP1I GCGC 2 cut(s) 565, 1106
HindIII AAGCTT 1 cut(s) 1056
HinfI GANTC 4 cut(s) 64, 352, 743, 754
HpaII CCGG 2 cut(s) 260, 624
HphI GGTGA 3 cut(s) 86, 793, 1283
Hpy166II GTNNAC 4 cut(s) 148, 437, 886, 1067
Hpy188I TCNGA 7 cut(s) 360, 543, 742, 815, 822, 909, 1252
Hpy8I GTNNAC 4 cut(s) 148, 437, 886, 1067
HpyAV CCTTC 6 cut(s) 199, 286, 322, 569, 667, 782
HpyCH4III ACNGT 2 cut(s) 877, 890
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 7 cut(s) 340, 368, 829, 1017, 1085, 1139, 1192
HpyF10VI GCNNNNNNNGC 3 cut(s) 599, 650, 1241
HpyF3I CTNAG 7 cut(s) 104, 168, 311, 348, 900, 952, 1101
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 7 cut(s) 368, 476, 599, 713, 796, 1190, 1196
HspAI GCGC 2 cut(s) 565, 1106
Ksp22I TGATCA 1 cut(s) 1288
Kzo9I GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
LmnI GCTCC 2 cut(s) 262, 459
Lsp1109I GCAGC 6 cut(s) 614, 628, 665, 982, 1123, 1190
LweI GCATC 2 cut(s) 19, 1072
MabI ACCWGGT 1 cut(s) 500
MaeI CTAG 3 cut(s) 80, 866, 1236
MaeII ACGT 1 cut(s) 323
MalI GATC 8 cut(s) 78, 393, 423, 549, 718, 1130, 1164, 1290
MboI GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
MboII GAAGA 9 cut(s) 256, 297, 1004, 1019, 1044, 1064, 1136, 1181, 1231
MfeI CAATTG 1 cut(s) 1076
MhlI GDGCHC 1 cut(s) 738
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 2 cut(s) 475, 1101
MseI TTAA 7 cut(s) 69, 459, 759, 894, 942, 1092, 1110
MslI CAYNNNNRTG 2 cut(s) 369, 1191
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 2 cut(s) 260, 624
MspR9I CCNGG 4 cut(s) 432, 502, 532, 624
MunI CAATTG 1 cut(s) 1076
MvaI CCWGG 3 cut(s) 432, 502, 532
MwoI GCNNNNNNNGC 3 cut(s) 599, 650, 1241
NciI CCSGG 1 cut(s) 624
NdeI CATATG 1 cut(s) 574
NdeII GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
NheI GCTAGC 1 cut(s) 865
NlaIII CATG 7 cut(s) 368, 476, 599, 713, 796, 1190, 1196
NlaIV GGNNCC 2 cut(s) 499, 681
NspI RCATGY 2 cut(s) 713, 1190
PagI TCATGA 1 cut(s) 472
PciI ACATGT 1 cut(s) 1186
PfeI GAWTC 3 cut(s) 64, 743, 754
PkrI GCNGC 7 cut(s) 220, 604, 643, 655, 972, 1138, 1180
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PpuMI RGGWCCY 2 cut(s) 534, 680
PscI ACATGT 1 cut(s) 1186
PshBI ATTAAT 1 cut(s) 1092
Psp124BI GAGCTC 1 cut(s) 738
Psp5II RGGWCCY 2 cut(s) 534, 680
Psp6I CCWGG 3 cut(s) 430, 500, 530
PspGI CCWGG 3 cut(s) 430, 500, 530
PspN4I GGNNCC 2 cut(s) 499, 681
PspPI GGNCC 3 cut(s) 534, 592, 680
PspPPI RGGWCCY 2 cut(s) 534, 680
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 3 cut(s) 662, 826, 875
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 2 cut(s) 369, 1191
SacI GAGCTC 1 cut(s) 738
SaqAI TTAA 7 cut(s) 69, 459, 759, 894, 942, 1092, 1110
SatI GCNGC 7 cut(s) 219, 603, 642, 654, 971, 1137, 1179
Sau3AI GATC 8 cut(s) 76, 391, 421, 547, 716, 1128, 1162, 1288
Sau96I GGNCC 3 cut(s) 534, 592, 680
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 4 cut(s) 432, 502, 532, 624
SduI GDGCHC 1 cut(s) 738
SexAI ACCWGGT 1 cut(s) 500
SfaNI GCATC 2 cut(s) 19, 1072
SfcI CTRYAG 1 cut(s) 827
SinI GGWCC 2 cut(s) 534, 680
SmiMI CAYNNNNRTG 2 cut(s) 369, 1191
SmlI CTYRAG 2 cut(s) 425, 731
SmoI CTYRAG 2 cut(s) 425, 731
SsiI CCGC 3 cut(s) 32, 219, 914
SspI AATATT 2 cut(s) 940, 1090
SspMI CTAG 3 cut(s) 80, 866, 1236
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 4 cut(s) 430, 500, 530, 622
TaaI ACNGT 2 cut(s) 877, 890
TaiI ACGT 1 cut(s) 326
TaqI TCGA 1 cut(s) 60
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 3 cut(s) 64, 743, 754
Tru1I TTAA 7 cut(s) 69, 459, 759, 894, 942, 1092, 1110
Tru9I TTAA 7 cut(s) 69, 459, 759, 894, 942, 1092, 1110
TscAI CASTG 3 cut(s) 616, 880, 895
TseI GCWGC 6 cut(s) 602, 641, 653, 970, 1136, 1178
TspDTI ATGAA 7 cut(s) 257, 293, 387, 591, 612, 663, 1020
TspRI CASTG 3 cut(s) 616, 880, 895
VpaK11BI GGWCC 2 cut(s) 534, 680
VspI ATTAAT 1 cut(s) 1092
XapI RAATTY 5 cut(s) 56, 374, 851, 1253, 1283
XceI RCATGY 2 cut(s) 713, 1190
XmiI GTMKAC 1 cut(s) 1066
XspI CTAG 3 cut(s) 80, 866, 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.