Rh5DG023000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006425
Physical Location & Seq
Reverse (-)
1631776 .. 1633845
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG023000.1

Sequence Viewer

Length: 447 bp
ATGGTAGATTCGGCTAAGGAAACGGTACGCCGCCTTAGATCGAAAGGATATTCCAGCTTAGATGAGGATGAAGCAATCACTCTAAAGGACAGCATTCACTTCCTTGGTAGGCTAGAAAGCACCCCCTCTCCCTTTAGGGAGAGGTTTCTTAACTTTGCTGAAGACATTCCAAGCCGTGTCCGCTGCCTCCGTCAAATGCAGGAAACATTGATTGATCAGCCAAATCTTGTAGAGCAAGAGAGACAATGCATAGCCGAAATTCAGAACAGAATGCAAGCCCGCGCTCTAAGAAACCAACAGTTTGATGAGGAAGAAGTGACGGTCATTTGTGAGAGGGCTAAGAGAGAAGAGGAGAAAGAGGTTCTACTCACCAGATTGGAGGACATTCGCTGCAATGTGACTGAAATTTCCACAGCCTCGTCTTATATAGATGGTGGGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.28

Weight (kDa)

5.02

Isoelectric Point (pI)

59.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 3 cut(s) 31, 181, 280
AcsI RAATTY 2 cut(s) 258, 405
AcuI CTGAAG 1 cut(s) 180
AfaI GTAC 1 cut(s) 27
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 183, 390
ApoI RAATTY 2 cut(s) 258, 405
Asp700I GAANNNNTTC 1 cut(s) 165
AspLEI GCGC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 170, 377
BccI CCATC 1 cut(s) 425
BceAI ACGGC 1 cut(s) 159
BclI TGATCA 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 1 cut(s) 113
BisI GCNGC 3 cut(s) 31, 184, 391
BlsI GCNGC 3 cut(s) 32, 185, 392
BpiI GAAGAC 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bse3DI GCAATG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 1 cut(s) 73
BseMI GCAATG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 170, 377
Bsh1236I CGCG 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 235
BsmI GAATGC 2 cut(s) 93, 276
Bsp143I GATC 3 cut(s) 38, 214, 441
BspACI CCGC 3 cut(s) 31, 181, 280
BspFNI CGCG 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 3 cut(s) 38, 214, 441
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 3 cut(s) 25, 300, 322
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 276, 280
BstDEI CTNAG 5 cut(s) 15, 35, 58, 287, 339
BstF5I GGATG 1 cut(s) 73
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 3 cut(s) 41, 217, 444
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 3 cut(s) 38, 214, 441
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 170, 377
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
BtsCI GGATG 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 276, 280
CfoI GCGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 26
CviJI RGCY 9 cut(s) 14, 57, 112, 174, 220, 254, 278, 338, 416
CviKI_1 RGCY 9 cut(s) 14, 57, 112, 174, 220, 254, 278, 338, 416
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 5 cut(s) 15, 35, 58, 287, 339
DpnI GATC 3 cut(s) 40, 216, 443
DpnII GATC 3 cut(s) 38, 214, 441
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 103
FaiI YATR 3 cut(s) 251, 426, 428
FauI CCCGC 1 cut(s) 287
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 31, 184, 391
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 3 cut(s) 31, 184, 391
FspBI CTAG 1 cut(s) 113
GlaI GCGC 1 cut(s) 283
GluI GCNGC 3 cut(s) 31, 184, 391
HhaI GCGC 1 cut(s) 284
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HinfI GANTC 1 cut(s) 8
HphI GGTGA 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 264, 446
HpyCH4III ACNGT 3 cut(s) 25, 300, 322
HpyCH4V TGCA 4 cut(s) 199, 249, 274, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 5 cut(s) 15, 35, 58, 287, 339
HspAI GCGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 3 cut(s) 38, 214, 441
LpnPI CCDG 3 cut(s) 67, 185, 385
Lsp1109I GCAGC 2 cut(s) 170, 377
MaeI CTAG 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 316, 397
MalI GATC 3 cut(s) 40, 216, 443
MboI GATC 3 cut(s) 38, 214, 441
MboII GAAGA 3 cut(s) 173, 323, 359
MflI RGATCY 1 cut(s) 441
MluCI AATT 2 cut(s) 258, 405
Mph1103I ATGCAT 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 150
MspA1I CMGCKG 1 cut(s) 183
Mva1269I GAATGC 2 cut(s) 93, 276
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 180
NdeII GATC 3 cut(s) 38, 214, 441
NmuCI GTSAC 2 cut(s) 316, 397
NsiI ATGCAT 1 cut(s) 251
PctI GAATGC 2 cut(s) 93, 276
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 3 cut(s) 32, 185, 392
PsuI RGATCY 1 cut(s) 441
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 3 cut(s) 31, 184, 391
Sau3AI GATC 3 cut(s) 38, 214, 441
SetI ASST 3 cut(s) 59, 146, 363
Sse9I AATT 2 cut(s) 258, 405
SsiI CCGC 3 cut(s) 31, 181, 280
SspMI CTAG 1 cut(s) 113
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 3 cut(s) 25, 300, 322
TaqI TCGA 1 cut(s) 41
TasI AATT 2 cut(s) 258, 405
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TseFI GTSAC 2 cut(s) 316, 397
TseI GCWGC 2 cut(s) 183, 390
Tsp45I GTSAC 2 cut(s) 316, 397
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 179
XapI RAATTY 2 cut(s) 258, 405
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.