Rh5BG024600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006425
Physical Location & Seq
Reverse (-)
1747090 .. 1748106
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG024600.1

Sequence Viewer

Length: 570 bp
ATGGCTTCATCATCGAGGACGAGGGATCGGTTTAGTATTGAATTTCAACAAGTTGAGCAAAGTATGATTGACCAAGAGGTGTTGAAGGAGTGCGTTGATGGAGTTGGAAACCCAACACCTGAGGTAGAAAACCAGCTCAAGACCAATTTGTCCTACCAGCGGGCCCTTCAGGCACGCCAGGGAGTGCCATATGGTGGTCTAATCCGCGTTCAATTGTTTCCTCCTCCTCCGCCCCGTGATGATTATAGGGCAACTGTGAGGGAGAGGTTTCTTAACTTTGCTGAAGACATTCCAAGCCGTGTCCGCTGCCTCCGTCAAATGCAGGAAACATTGATTGATCAGCCAAATCTTGTAGAGCAAGAGAGACAATGCATAGCCGAAATTCAGAACAGAATGCAAGCCCGTGCTCTAAGAAACCAACAGTTAGATGAGGAAGAAGTGACGGTCACTTGTGAGAGGGCTAAGAGAGAAGAGGAGAAAGAGGTTCTAATCACCAGATTGGAGGACGTTCGCTGCAATGTGACTGAAATTTCCACAGCCTCGTCTTATATAGATGGTGGGAGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.89

Weight (kDa)

4.99

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccB7I CCANNNNNTGG 1 cut(s) 194
AccII CGCG 1 cut(s) 207
AciI CCGC 4 cut(s) 160, 205, 230, 304
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 3 cut(s) 41, 381, 528
AcuI CTGAAG 2 cut(s) 152, 303
AfiI CCNNNNNNNGG 2 cut(s) 159, 194
AgsI TTSAA 4 cut(s) 41, 47, 85, 212
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 1 cut(s) 33
AoxI GGCC 1 cut(s) 162
ApaI GGGCCC 1 cut(s) 166
ApeKI GCWGC 2 cut(s) 306, 513
ApoI RAATTY 3 cut(s) 41, 381, 528
Asp700I GAANNNNTTC 1 cut(s) 288
AspS9I GGNCC 2 cut(s) 162, 163
AsuHPI GGTGA 1 cut(s) 484
AxyI CCTNAGG 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 166
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 293, 500
BccI CCATC 2 cut(s) 92, 548
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 2 cut(s) 307, 514
BlsI GCNGC 2 cut(s) 308, 515
Bme1390I CCNGG 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 162, 163
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 178
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 194
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 159, 194
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 1 cut(s) 111
BseRI GAGGAG 3 cut(s) 213, 216, 488
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 293, 500
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 409
BslI CCNNNNNNNGG 2 cut(s) 159, 194
BsmAI GTCTC 1 cut(s) 358
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 1 cut(s) 164
Bsp120I GGGCCC 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 166, 409
Bsp143I GATC 3 cut(s) 25, 337, 564
BspACI CCGC 4 cut(s) 160, 205, 230, 304
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 1 cut(s) 112
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 523
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 3 cut(s) 25, 337, 564
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 256, 423, 445
Bst6I CTCTTC 1 cut(s) 465
BstC8I GCNNGC 3 cut(s) 162, 175, 399
BstDEI CTNAG 3 cut(s) 120, 410, 462
BstFNI CGCG 1 cut(s) 207
BstKTI GATC 3 cut(s) 28, 340, 567
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 25, 337, 564
BstMWI GCNNNNNNNGC 2 cut(s) 170, 303
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 293, 500
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 162, 175, 399
Cfr13I GGNCC 2 cut(s) 162, 163
CviJI RGCY 9 cut(s) 5, 136, 164, 297, 343, 377, 401, 461, 539
CviKI_1 RGCY 9 cut(s) 5, 136, 164, 297, 343, 377, 401, 461, 539
DdeI CTNAG 3 cut(s) 120, 410, 462
DpnI GATC 3 cut(s) 27, 339, 566
DpnII GATC 3 cut(s) 25, 337, 564
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 152, 303
Eco81I CCTNAGG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 166
FaiI YATR 7 cut(s) 65, 190, 192, 246, 374, 549, 551
FauI CCCGC 1 cut(s) 153
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 2 cut(s) 307, 514
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 2 cut(s) 307, 514
GluI GCNGC 2 cut(s) 307, 514
HaeIII GGCC 1 cut(s) 164
HphI GGTGA 1 cut(s) 484
Hpy188I TCNGA 2 cut(s) 387, 569
Hpy188III TCNNGA 1 cut(s) 139
HpyAV CCTTC 2 cut(s) 79, 176
HpyCH4III ACNGT 3 cut(s) 256, 423, 445
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 322, 372, 397, 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 303
HpyF3I CTNAG 3 cut(s) 120, 410, 462
HpySE526I ACGT 1 cut(s) 507
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 3 cut(s) 25, 337, 564
LpnPI CCDG 8 cut(s) 132, 146, 155, 164, 170, 191, 308, 508
Lsp1109I GCAGC 2 cut(s) 293, 500
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 439, 445, 520
MalI GATC 3 cut(s) 27, 339, 566
MboI GATC 3 cut(s) 25, 337, 564
MboII GAAGA 3 cut(s) 296, 446, 482
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 166, 409
MluCI AATT 5 cut(s) 41, 145, 212, 381, 528
MmeI TCCRAC 1 cut(s) 85
Mph1103I ATGCAT 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 160, 306
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 170, 303
NdeI CATATG 1 cut(s) 190
NdeII GATC 3 cut(s) 25, 337, 564
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 3 cut(s) 439, 445, 520
NsiI ATGCAT 1 cut(s) 374
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 288
PflMI CCANNNNNTGG 1 cut(s) 194
PkrI GCNGC 2 cut(s) 308, 515
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 164
PspOMI GGGCCC 1 cut(s) 162
PspPI GGNCC 2 cut(s) 162, 163
PsuI RGATCY 1 cut(s) 564
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 2 cut(s) 307, 514
Sau3AI GATC 3 cut(s) 25, 337, 564
Sau96I GGNCC 2 cut(s) 162, 163
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 166, 409
SetI ASST 7 cut(s) 81, 121, 126, 138, 269, 486, 510
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 5 cut(s) 41, 145, 212, 381, 528
SsiI CCGC 4 cut(s) 160, 205, 230, 304
StyD4I CCNGG 1 cut(s) 177
TaaI ACNGT 3 cut(s) 256, 423, 445
TaiI ACGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 14
TasI AATT 5 cut(s) 41, 145, 212, 381, 528
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseFI GTSAC 3 cut(s) 439, 445, 520
TseI GCWGC 2 cut(s) 306, 513
Tsp45I GTSAC 3 cut(s) 439, 445, 520
TspGWI ACGGA 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 194
XapI RAATTY 3 cut(s) 41, 381, 528
XmnI GAANNNNTTC 1 cut(s) 288
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.