Rw1G023910

Lariat debranching

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002005
Physical Location & Seq
Forward (+)
49923155 .. 49931846
8692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023910.1

Sequence Viewer

Length: 984 bp
ATGAGAGCTTCGAGTGAATTCGATTCCATCGGTTTGGCTGTGAGGAATGAGAAGGATTTGGAGAGCTTGAGTGTGCCCCCCAAGTACCGGTCCATGAACTCCTTCTGGAAGTACTATTCCGGCGAGGAGATCGCTCCCTACCCCACCATATTCATCGGTGGCAATCACGAAGCTTCTAATTATTTGTGGGAACTGTACTACGGTGGGTGGGCGGCACCTAATATATACTTCTTGTGGTTTTCTGGGGTAGTCAAGTTCGGAAACCTTCGCATTGGTGGCCTCTCTGGAATTTATAAGTCGCGAGACTTCGTTCAGGACACTATGAGAGGCTCTGTATATCATGTTCGTGAGTATGATGTCCACAAACTCATGCAAGTTGAGGAACCAATCGATATTTTTCTTTCACATGATTGGCCTCTTGGCATCACGGATTGTGGGGACTGGAAGCAACTTGTTCGGTACAAACCTCAGTTTGAGACCGAGGTTCGGGAAACAACCCTGGGAAGTAAACCTGCTGCTCAATTGCTAGAAAAATTGAAACCACCCTATTGGTTTTCACATGGGGAAGATGGTCCCGTGACGAATTTTCTTGCTCTTGATAAGTGTCTTCCTGGAAAGAAGTTTTTGCAGGAGATTCAGTATGATGAAGAATGGCTAGAAATAACTCGAAGGTTTAACCCCATTTCCCCTCTCACAATCAGGAGTGCAAATTTCAGGGGCGTACATATTGACAAGCAAGATTGTCGTCAATGGGTAAGGAGGAAGCTACAAGCAAGAGGAGCCCGACCTTTTGAATTCAGCCAGACAGCTCCATCTTATAATCCCTCTAATTCTGTCTCAAATGGCTCCTCGCCTGGATATACTCGGAATCCTCAAACAGAATCTTTGTTGCAGTTTCTGGAACTCCCTTATCTTCTTGATGACATATGTCAGAATCTTCGGAAGTATCCAGGAGTCCTTTTCCATCAAGCGATAGAGGAGTGA

Protein Analysis

327

Amino Acids

37.88

Weight (kDa)

6.21

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBR1 PF05011 193 - 303 3.6e-18 Lariat debranching enzyme, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 294, 819
Acc36I ACCTGC 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 214
AccII CGCG 1 cut(s) 301
AciI CCGC 1 cut(s) 212
AcsI RAATTY 5 cut(s) 17, 288, 583, 709, 794
AfaI GTAC 5 cut(s) 86, 113, 197, 461, 723
AfiI CCNNNNNNNGG 2 cut(s) 87, 486
AgeI ACCGGT 1 cut(s) 87
AgsI TTSAA 2 cut(s) 538, 794
AjnI CCWGG 4 cut(s) 498, 610, 853, 949
AluBI AGCT 5 cut(s) 8, 66, 173, 766, 809
AluI AGCT 5 cut(s) 8, 66, 173, 766, 809
Alw26I GTCTC 3 cut(s) 297, 470, 841
AlwNI CAGNNNCTG 1 cut(s) 898
AoxI GGCC 2 cut(s) 277, 413
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 5 cut(s) 17, 288, 583, 709, 794
AsiGI ACCGGT 1 cut(s) 87
Asp700I GAANNNNTTC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 90, 572
AvaII GGWCC 2 cut(s) 90, 572
BaeGI GKGCMC 1 cut(s) 78
BanI GGYRCC 1 cut(s) 214
BanII GRGCYC 1 cut(s) 784
BbsI GAAGAC 1 cut(s) 599
BbvI GCAGC 1 cut(s) 502
BccI CCATC 4 cut(s) 35, 563, 820, 972
BcgI CGANNNNNNTGC 4 cut(s) 437, 471, 725, 759
BciT130I CCWGG 4 cut(s) 500, 612, 855, 951
BciVI GTATCC 1 cut(s) 957
BcoDI GTCTC 3 cut(s) 297, 470, 841
BfaI CTAG 2 cut(s) 527, 656
BfuAI ACCTGC 1 cut(s) 520
BfuI GTATCC 1 cut(s) 957
BisI GCNGC 2 cut(s) 213, 516
BlsI GCNGC 2 cut(s) 214, 517
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 4 cut(s) 500, 612, 855, 951
Bme18I GGWCC 2 cut(s) 90, 572
BmgT120I GGNCC 2 cut(s) 90, 572
BmiI GGNNCC 5 cut(s) 216, 384, 574, 781, 847
BmrFI CCNGG 4 cut(s) 500, 612, 855, 951
BmsI GCATC 1 cut(s) 432
BoxI GACNNNNGTC 1 cut(s) 927
BpiI GAAGAC 1 cut(s) 599
BpuEI CTTGAG 1 cut(s) 88
Bsa29I ATCGAT 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 480, 498, 499
BsaWI WCCGGW 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 486
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 446
BseBI CCWGG 4 cut(s) 500, 612, 855, 951
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 480, 498, 499
BseLI CCNNNNNNNGG 2 cut(s) 87, 486
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 446
BseRI GAGGAG 3 cut(s) 140, 792, 838
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 279, 415
BshNI GGYRCC 1 cut(s) 214
BshTI ACCGGT 1 cut(s) 87
BshVI ATCGAT 1 cut(s) 390
BsiSI CCGG 2 cut(s) 88, 120
BslFI GGGAC 2 cut(s) 452, 558
BslI CCNNNNNNNGG 2 cut(s) 87, 486
BsmAI GTCTC 3 cut(s) 297, 470, 841
BsmFI GGGAC 2 cut(s) 452, 558
BsnI GGCC 2 cut(s) 279, 415
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 78, 784
Bsp143I GATC 1 cut(s) 129
Bsp68I TCGCGA 1 cut(s) 301
BspACI CCGC 1 cut(s) 212
BspANI GGCC 2 cut(s) 279, 415
BspCNI CTCAG 1 cut(s) 481
BspDI ATCGAT 1 cut(s) 390
BspFNI CGCG 1 cut(s) 301
BspLI GGNNCC 5 cut(s) 216, 384, 574, 781, 847
BspMI ACCTGC 1 cut(s) 520
BspT107I GGYRCC 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 1 cut(s) 446
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 3 cut(s) 480, 498, 499
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 4 cut(s) 500, 612, 855, 951
Bst4CI ACNGT 2 cut(s) 195, 203
BstDEI CTNAG 1 cut(s) 468
BstFNI CGCG 1 cut(s) 301
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 3 cut(s) 297, 470, 841
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 276, 779
BstNI CCWGG 4 cut(s) 500, 612, 855, 951
BstPAI GACNNNNGTC 1 cut(s) 927
BstSCI CCNGG 4 cut(s) 498, 610, 853, 949
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 599
BstXI CCANNNNNNTGG 2 cut(s) 34, 549
Bsu15I ATCGAT 1 cut(s) 390
BsuI GTATCC 1 cut(s) 957
BsuRI GGCC 2 cut(s) 279, 415
BsuTUI ATCGAT 1 cut(s) 390
BtuMI TCGCGA 1 cut(s) 301
BveI ACCTGC 1 cut(s) 520
CaiI CAGNNNCTG 1 cut(s) 898
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 90, 572
ClaI ATCGAT 1 cut(s) 390
Csp6I GTAC 5 cut(s) 85, 112, 196, 460, 722
CspAI ACCGGT 1 cut(s) 87
CviAII CATG 5 cut(s) 94, 341, 370, 407, 560
CviQI GTAC 5 cut(s) 85, 112, 196, 460, 722
DdeI CTNAG 1 cut(s) 468
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 784
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 90, 572
EcoRI GAATTC 2 cut(s) 17, 794
EcoRII CCWGG 4 cut(s) 498, 610, 853, 949
EcoT38I GRGCYC 1 cut(s) 784
FaeI CATG 5 cut(s) 97, 344, 373, 410, 563
FaqI GGGAC 2 cut(s) 452, 558
FatI CATG 5 cut(s) 93, 340, 369, 406, 559
FauNDI CATATG 1 cut(s) 926
Fnu4HI GCNGC 2 cut(s) 213, 516
FriOI GRGCYC 1 cut(s) 784
Fsp4HI GCNGC 2 cut(s) 213, 516
FspBI CTAG 2 cut(s) 527, 656
GluI GCNGC 2 cut(s) 213, 516
HaeIII GGCC 2 cut(s) 279, 415
HapII CCGG 2 cut(s) 88, 120
Hin1II CATG 5 cut(s) 97, 344, 373, 410, 563
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 6 cut(s) 23, 634, 868, 881, 934, 954
HpaII CCGG 2 cut(s) 88, 120
Hpy166II GTNNAC 2 cut(s) 361, 509
Hpy188I TCNGA 4 cut(s) 260, 867, 933, 942
Hpy8I GTNNAC 2 cut(s) 361, 509
HpyAV CCTTC 4 cut(s) 46, 112, 275, 663
HpyCH4III ACNGT 2 cut(s) 195, 203
HpyCH4V TGCA 4 cut(s) 373, 628, 707, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 779
HpyF3I CTNAG 1 cut(s) 468
Hsp92II CATG 5 cut(s) 97, 344, 373, 410, 563
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 4 cut(s) 139, 779, 814, 851
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 1 cut(s) 432
MaeI CTAG 2 cut(s) 527, 656
MaeIII GTNAC 1 cut(s) 577
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 5 cut(s) 578, 599, 659, 905, 929
MfeI CAATTG 1 cut(s) 521
MhlI GDGCHC 2 cut(s) 78, 784
MluCI AATT 9 cut(s) 17, 178, 288, 521, 533, 583, 709, 794, 829
MlyI GAGTC 1 cut(s) 963
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 2 cut(s) 88, 120
MspR9I CCNGG 4 cut(s) 500, 612, 855, 951
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 4 cut(s) 500, 612, 855, 951
MvnI CGCG 1 cut(s) 301
MwoI GCNNNNNNNGC 2 cut(s) 276, 779
NdeI CATATG 1 cut(s) 926
NdeII GATC 1 cut(s) 129
NlaIII CATG 5 cut(s) 97, 344, 373, 410, 563
NlaIV GGNNCC 5 cut(s) 216, 384, 574, 781, 847
NmuCI GTSAC 1 cut(s) 577
NruI TCGCGA 1 cut(s) 301
PasI CCCWGGG 1 cut(s) 499
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 5 cut(s) 23, 634, 868, 881, 934
PfoI TCCNGGA 2 cut(s) 610, 949
PinAI ACCGGT 1 cut(s) 87
PkrI GCNGC 2 cut(s) 214, 517
PleI GAGTC 1 cut(s) 962
PpsI GAGTC 1 cut(s) 962
PshAI GACNNNNGTC 1 cut(s) 927
PsiI TTATAA 2 cut(s) 294, 819
Psp6I CCWGG 4 cut(s) 498, 610, 853, 949
PspGI CCWGG 4 cut(s) 498, 610, 853, 949
PspN4I GGNNCC 5 cut(s) 216, 384, 574, 781, 847
PspPI GGNCC 2 cut(s) 90, 572
PsrI GAACNNNNNNTAC 2 cut(s) 327, 359
PstNI CAGNNNCTG 1 cut(s) 898
RruI TCGCGA 1 cut(s) 301
RsaI GTAC 5 cut(s) 86, 113, 197, 461, 723
RsaNI GTAC 5 cut(s) 85, 112, 196, 460, 722
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 2 cut(s) 213, 516
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 2 cut(s) 90, 572
ScaI AGTACT 1 cut(s) 113
SchI GAGTC 1 cut(s) 963
ScrFI CCNGG 4 cut(s) 500, 612, 855, 951
SduI GDGCHC 2 cut(s) 78, 784
SfaNI GCATC 1 cut(s) 432
SinI GGWCC 2 cut(s) 90, 572
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 9 cut(s) 17, 178, 288, 521, 533, 583, 709, 794, 829
SsiI CCGC 1 cut(s) 212
SspMI CTAG 2 cut(s) 527, 656
StyD4I CCNGG 4 cut(s) 498, 610, 853, 949
TaaI ACNGT 2 cut(s) 195, 203
TaqI TCGA 4 cut(s) 11, 21, 390, 667
TaqII GACCGA 1 cut(s) 494
TasI AATT 9 cut(s) 17, 178, 288, 521, 533, 583, 709, 794, 829
TatI WGTACW 2 cut(s) 111, 195
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 5 cut(s) 23, 634, 868, 881, 934
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 3 cut(s) 110, 142, 660
TspGWI ACGGA 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 90, 572
XapI RAATTY 5 cut(s) 17, 288, 583, 709, 794
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 2 cut(s) 527, 656
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.