AT1G53540

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003719
Physical Location & Seq
Forward (+)
19980299 .. 19981199
901 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53540.1

Sequence Viewer

Length: 474 bp
ATGTCTCTAATTCCAAGCATCTTCGGAGGAAGAAGAACAAACGTTTTCGATCCGTTCTCGCTGGATGTTTTCGATCCGTTCGAAGGTTTCTTGACGCCGTCAGGATTGGCAAACGCACCCGCTATGGATGTGGCAGCGTTCACAAACGCTAAAGTGGATTGGAGGGAGACACCTGAGGCGCACGTGTTCAAGGCGGATTTACCGGGACTGAGGAAGGAAGAGGTGAAAGTGGAGGTTGAGGATGGCAACATACTTCAGATAAGTGGAGAGAGGAGCAATGAGAATGAAGAGAAGAATGACAAGTGGCACCGTGTGGAGCGGTCGAGTGGGAAGTTCACGAGGAGGTTTAGGCTGCCGGAGAATGCTAAGATGGAGGAAATCAAAGCCAGTATGGAAAATGGTGTGTTGTCGGTTACCGTGCCCAAGGTGCCAGAGAAAAAGCCAGAGGTTAAGTCCATTGATATCTCTGGTTAA

Protein Analysis

157

Amino Acids

17.6

Weight (kDa)

5.36

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 53 - 156 1.9e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 306, 427
AccBSI CCGCTC 1 cut(s) 319
AciI CCGC 3 cut(s) 120, 194, 319
AclI AACGTT 1 cut(s) 42
AclWI GGATC 2 cut(s) 44, 68
AcuI CTGAAG 1 cut(s) 239
AcvI CACGTG 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 95
AdeI CACNNNGTG 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 9, 161
AlwI GGATC 2 cut(s) 44, 68
ApeKI GCWGC 2 cut(s) 134, 352
AspLEI GCGC 1 cut(s) 181
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 1 cut(s) 235
AsuII TTCGAA 1 cut(s) 81
AxyI CCTNAGG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 423
BanI GGYRCC 2 cut(s) 306, 427
BauI CACGAG 1 cut(s) 337
BbrPI CACGTG 1 cut(s) 184
BbvI GCAGC 2 cut(s) 146, 339
BccI CCATC 2 cut(s) 236, 364
BceAI ACGGC 1 cut(s) 82
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 2 cut(s) 9, 161
BisI GCNGC 2 cut(s) 135, 353
BlsI GCNGC 2 cut(s) 136, 354
Bme1390I CCNGG 1 cut(s) 204
BmiI GGNNCC 2 cut(s) 308, 429
BmrFI CCNGG 1 cut(s) 204
BmsI GCATC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 387
Bse21I CCTNAGG 1 cut(s) 174
Bse3DI GCAATG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 3 cut(s) 70, 133, 247
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 165, 200
BseNI ACTGG 1 cut(s) 387
BseRI GAGGAG 2 cut(s) 286, 355
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 2 cut(s) 146, 339
Bsh1285I CGRYCG 1 cut(s) 323
BshNI GGYRCC 2 cut(s) 306, 427
BsiEI CGRYCG 1 cut(s) 323
BsiSI CCGG 2 cut(s) 203, 356
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 9, 161
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 2 cut(s) 49, 73
BspACI CCGC 3 cut(s) 120, 194, 319
BspCNI CTCAG 2 cut(s) 166, 201
BspLI GGNNCC 2 cut(s) 308, 429
BspPI GGATC 2 cut(s) 44, 68
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 306, 427
BsrBI CCGCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 2 cut(s) 49, 73
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 311, 418
Bst6I CTCTTC 2 cut(s) 213, 282
BstACI GRCGYC 1 cut(s) 95
BstBAI YACGTR 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 81
BstDEI CTNAG 3 cut(s) 174, 209, 366
BstEII GGTNACC 1 cut(s) 412
BstF5I GGATG 3 cut(s) 70, 133, 247
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 2 cut(s) 52, 76
BstMAI GTCTC 2 cut(s) 9, 161
BstMBI GATC 2 cut(s) 49, 73
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 202
BstSLI GKGCMC 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 146, 339
Bsu36I CCTNAGG 1 cut(s) 174
BtsCI GGATG 3 cut(s) 70, 133, 247
CfoI GCGC 1 cut(s) 181
CseI GACGC 1 cut(s) 103
CviJI RGCY 3 cut(s) 352, 386, 442
CviKI_1 RGCY 3 cut(s) 352, 386, 442
DdeI CTNAG 3 cut(s) 174, 209, 366
DpnI GATC 2 cut(s) 51, 75
DpnII GATC 2 cut(s) 49, 73
DraIII CACNNNGTG 1 cut(s) 313
Eam1104I CTCTTC 2 cut(s) 213, 282
EarI CTCTTC 2 cut(s) 213, 282
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 423
Eco32I GATATC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 239
Eco72I CACGTG 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 412
EcoO65I GGTNACC 1 cut(s) 412
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
FaiI YATR 3 cut(s) 125, 251, 392
FaqI GGGAC 1 cut(s) 219
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 135, 353
FokI GGATG 3 cut(s) 77, 140, 254
Fsp4HI GCNGC 2 cut(s) 135, 353
GlaI GCGC 1 cut(s) 180
GluI GCNGC 2 cut(s) 135, 353
HapII CCGG 2 cut(s) 203, 356
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 181
Hin1I GRCGYC 1 cut(s) 95
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HpaII CCGG 2 cut(s) 203, 356
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 141, 336
Hpy188I TCNGA 2 cut(s) 26, 258
Hpy188III TCNNGA 3 cut(s) 91, 102, 337
Hpy8I GTNNAC 2 cut(s) 141, 336
HpyAV CCTTC 2 cut(s) 77, 208
HpyCH4III ACNGT 2 cut(s) 311, 418
HpyCH4IV ACGT 2 cut(s) 42, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpyF3I CTNAG 3 cut(s) 174, 209, 366
HpySE526I ACGT 2 cut(s) 42, 183
Hsp92I GRCGYC 1 cut(s) 95
HspAI GCGC 1 cut(s) 179
Kzo9I GATC 2 cut(s) 49, 73
LmnI GCTCC 2 cut(s) 273, 316
LpnPI CCDG 9 cut(s) 47, 87, 186, 216, 369, 400, 444, 453, 456
Lsp1109I GCAGC 2 cut(s) 146, 339
LweI GCATC 1 cut(s) 27
MaeII ACGT 2 cut(s) 42, 183
MaeIII GTNAC 1 cut(s) 412
MalI GATC 2 cut(s) 51, 75
MbiI CCGCTC 1 cut(s) 319
MboI GATC 2 cut(s) 49, 73
MboII GAAGA 6 cut(s) 13, 42, 45, 230, 299, 304
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 1 cut(s) 9
MseI TTAA 2 cut(s) 450, 472
MspI CCGG 2 cut(s) 203, 356
MspR9I CCNGG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 427
NciI CCSGG 1 cut(s) 204
NdeII GATC 2 cut(s) 49, 73
NlaIV GGNNCC 2 cut(s) 308, 429
NspV TTCGAA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 1 cut(s) 367
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 136, 354
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 184
Psp1406I AACGTT 1 cut(s) 42
PspCI CACGTG 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 308, 429
PsyI GACNNNGTC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 450, 472
SatI GCNGC 2 cut(s) 135, 353
Sau3AI GATC 2 cut(s) 49, 73
ScrFI CCNGG 1 cut(s) 204
SduI GDGCHC 1 cut(s) 423
SetI ASST 9 cut(s) 45, 88, 175, 186, 225, 237, 347, 429, 450
SfaNI GCATC 1 cut(s) 27
SfuI TTCGAA 1 cut(s) 81
Sse9I AATT 1 cut(s) 9
SsiI CCGC 3 cut(s) 120, 194, 319
StyD4I CCNGG 1 cut(s) 202
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 311, 418
TaiI ACGT 2 cut(s) 45, 186
TaqI TCGA 4 cut(s) 48, 72, 81, 323
TasI AATT 1 cut(s) 9
Tru1I TTAA 2 cut(s) 450, 472
Tru9I TTAA 2 cut(s) 450, 472
TseI GCWGC 2 cut(s) 134, 352
TspDTI ATGAA 1 cut(s) 300
TspGWI ACGGA 2 cut(s) 42, 66
Tth111I GACNNNGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.