Rroxscaffold_4G00288100

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003719
Physical Location & Seq
Reverse (-)
8990680 .. 8991578
899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288100.1

Sequence Viewer

Length: 471 bp
ATGGCTCTCAGTCTCTTTGGCACCGGCCGACGCAGCAACGTCTTCGACCCATTTTCCCTCGACATCTGGGACCCCTTTCAGGGCATAGGCTCTCTCGTCAACTCCTCGTCCACTGCCGGCGACACCTCCGCCTTCGCTCAGACCCGAATCGACTGGAAGGAGACCCCGGAGGCCCACATCTTCAAGGCGGACCTCCCTGGACTGAAGAAGGAAGAAGTGAAGGTTGAGTTGGAGGAAGGGAATGTCCTTCAGATCAGCGGAGAGAGGAGCAGGGAGCAGGAGGAGAAGAATGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAATTCCTGAGGAGGTTCAGGCTGCCGGATAATGCAAAGGTGGATCATGTCAGGGCTACTATGGAGAACGGGGTTCTTACTGTGACTGTGCCCAAAGCAGAGGAGCCGAAGCCCCAGGTCAAGAGCATCGACATTACTGGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

156

Amino Acids

17.48

Weight (kDa)

5.97

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 154 3e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AciI CCGC 4 cut(s) 129, 188, 258, 321
AclWI GGATC 1 cut(s) 375
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 2 cut(s) 224, 233
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 79, 80
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 2 cut(s) 196, 438
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
Alw26I GTCTC 2 cut(s) 17, 155
AlwI GGATC 1 cut(s) 375
AoxI GGCC 2 cut(s) 25, 171
ApeKI GCWGC 3 cut(s) 33, 318, 346
ApoI RAATTY 1 cut(s) 326
AspS9I GGNCC 3 cut(s) 70, 172, 190
AsuC2I CCSGG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 70, 190
AxyI CCTNAGG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 34
BbvI GCAGC 3 cut(s) 45, 330, 333
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 2 cut(s) 198, 440
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 2 cut(s) 17, 155
BisI GCNGC 4 cut(s) 34, 319, 322, 347
BlsI GCNGC 4 cut(s) 35, 320, 323, 348
Bme1390I CCNGG 3 cut(s) 167, 198, 440
Bme18I GGWCC 2 cut(s) 70, 190
BmgT120I GGNCC 3 cut(s) 70, 172, 190
BmiI GGNNCC 5 cut(s) 22, 71, 72, 429, 466
BmrFI CCNGG 3 cut(s) 167, 198, 440
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 165, 196, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 80
Bse118I RCCGGY 2 cut(s) 23, 116
Bse1I ACTGG 2 cut(s) 158, 466
Bse21I CCTNAGG 1 cut(s) 332
BseBI CCWGG 2 cut(s) 198, 440
BseDI CCNNGG 3 cut(s) 165, 196, 438
BseLI CCNNNNNNNGG 2 cut(s) 79, 80
BseMII CTCAG 3 cut(s) 22, 152, 323
BseNI ACTGG 2 cut(s) 158, 466
BseRI GAGGAG 6 cut(s) 94, 280, 296, 328, 349, 440
BseSI GKGCMC 1 cut(s) 417
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 45, 330, 333
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 27, 173
BshNI GGYRCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 4 cut(s) 24, 117, 167, 350
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 79, 80
BsmAI GTCTC 2 cut(s) 17, 155
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 27, 173
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 2 cut(s) 252, 367
BspACI CCGC 4 cut(s) 129, 188, 258, 321
BspANI GGCC 2 cut(s) 27, 173
BspCNI CTCAG 3 cut(s) 21, 151, 324
BspLI GGNNCC 5 cut(s) 22, 71, 72, 429, 466
BspPI GGATC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 155
BsrFI RCCGGY 2 cut(s) 23, 116
BsrI ACTGG 2 cut(s) 158, 466
BssAI RCCGGY 2 cut(s) 23, 116
BssECI CCNNGG 3 cut(s) 165, 196, 438
BssMI GATC 2 cut(s) 252, 367
Bst2UI CCWGG 2 cut(s) 198, 440
Bst4CI ACNGT 2 cut(s) 406, 412
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 3 cut(s) 8, 138, 332
BstKTI GATC 2 cut(s) 255, 370
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 2 cut(s) 252, 367
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 2 cut(s) 198, 440
BstSCI CCNGG 3 cut(s) 165, 196, 438
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 45, 330, 333
BstV2I GAAGAC 1 cut(s) 34
BstZI CGGCCG 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 2 cut(s) 27, 173
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 118
Cfr10I RCCGGY 2 cut(s) 23, 116
Cfr13I GGNCC 3 cut(s) 70, 172, 190
CseI GACGC 1 cut(s) 39
CviAII CATG 1 cut(s) 371
CviJI RGCY 9 cut(s) 5, 27, 90, 173, 346, 380, 430, 436, 467
CviKI_1 RGCY 9 cut(s) 5, 27, 90, 173, 346, 380, 430, 436, 467
DdeI CTNAG 3 cut(s) 8, 138, 332
DpnI GATC 2 cut(s) 254, 369
DpnII GATC 2 cut(s) 252, 367
DraIII CACNNNGTG 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
EciI GGCGGA 2 cut(s) 118, 203
EclXI CGGCCG 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 70, 190
Eco52I CGGCCG 1 cut(s) 25
Eco57I CTGAAG 2 cut(s) 224, 233
Eco81I CCTNAGG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 196, 438
FaeI CATG 1 cut(s) 374
FaiI YATR 3 cut(s) 86, 372, 386
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 370
Fnu4HI GCNGC 4 cut(s) 34, 319, 322, 347
Fsp4HI GCNGC 4 cut(s) 34, 319, 322, 347
GluI GCNGC 4 cut(s) 34, 319, 322, 347
HaeIII GGCC 2 cut(s) 27, 173
HapII CCGG 4 cut(s) 24, 117, 167, 350
HgaI GACGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 374
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 147
HpaII CCGG 4 cut(s) 24, 117, 167, 350
Hpy166II GTNNAC 2 cut(s) 100, 111
Hpy188I TCNGA 2 cut(s) 141, 252
Hpy188III TCNNGA 2 cut(s) 331, 445
Hpy8I GTNNAC 2 cut(s) 100, 111
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 6 cut(s) 142, 151, 202, 214, 230, 257
HpyCH4III ACNGT 2 cut(s) 406, 412
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 3 cut(s) 8, 138, 332
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 1 cut(s) 374
KflI GGGWCCC 1 cut(s) 70
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 252, 367
LmnI GCTCC 5 cut(s) 267, 274, 315, 427, 464
Lsp1109I GCAGC 3 cut(s) 45, 330, 333
LweI GCATC 1 cut(s) 459
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 406
MalI GATC 2 cut(s) 254, 369
MboI GATC 2 cut(s) 252, 367
MboII GAAGA 5 cut(s) 34, 172, 217, 224, 298
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 210
MroNI GCCGGC 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 258, 321
MspI CCGG 4 cut(s) 24, 117, 167, 350
MspR9I CCNGG 3 cut(s) 167, 198, 440
MvaI CCWGG 2 cut(s) 198, 440
MwoI GCNNNNNNNGC 1 cut(s) 33
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 167
NdeII GATC 2 cut(s) 252, 367
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 374
NlaIV GGNNCC 5 cut(s) 22, 71, 72, 429, 466
NmuCI GTSAC 1 cut(s) 406
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 4 cut(s) 35, 320, 323, 348
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 196, 438
PspGI CCWGG 2 cut(s) 196, 438
PspN4I GGNNCC 5 cut(s) 22, 71, 72, 429, 466
PspPI GGNCC 3 cut(s) 70, 172, 190
PspPPI RGGWCCY 1 cut(s) 70
SatI GCNGC 4 cut(s) 34, 319, 322, 347
Sau3AI GATC 2 cut(s) 252, 367
Sau96I GGNCC 3 cut(s) 70, 172, 190
ScrFI CCNGG 3 cut(s) 167, 198, 440
SduI GDGCHC 1 cut(s) 417
SetI ASST 7 cut(s) 42, 128, 195, 225, 341, 366, 444
SfaNI GCATC 1 cut(s) 459
SinI GGWCC 2 cut(s) 70, 190
Sse9I AATT 1 cut(s) 326
SsiI CCGC 4 cut(s) 129, 188, 258, 321
StyD4I CCNGG 3 cut(s) 165, 196, 438
TaaI ACNGT 2 cut(s) 406, 412
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 45, 60, 150, 453
TasI AATT 1 cut(s) 326
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 1 cut(s) 147
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 3 cut(s) 33, 318, 346
Tsp45I GTSAC 1 cut(s) 406
TspRI CASTG 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 70, 190
XapI RAATTY 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.