MD01G1144400.v1.1

kDa class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003719
Physical Location & Seq
Forward (+)
25475100 .. 25479690
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1144400.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGTACCTAATTTTATAATACCAATTTGGAGTCTTTTTATAAGTAACAATTATGTTTTGGGCCGAAAAGGAACAAACCCAAAACAAGGCACTTTCAAGAAGGTGGTAGAAGCTGATGGAACTCTCTGGTTTTTTTTGTTTTATCCCTTCTGGATAGCTGCAGAACTTTCTGTAGTTCTCAGAGAAACCTTTTCTCAGAGAAACCTTCTGTGCCGTCCTATATATATACAAGTCACTCCGGTACCCACCCCATCAGATAGAATCATCCAAAGAAATATAAGCAAAGATCTCTCACTCAAATCCTTCGAATTCACACCCAGCAAAAACCCAACTCTAATCCGGCATCTAATGGCTCTCAGTCTCTTTGGCGGACGACGAAACAACATCTTCGACCCCTTCTCTCTGGACATCTGGGACCCATTCGAGGGCTTCGGCCCTCTGGCCAACTTCCCCTCCTCCGCCCGTGAAACCACCGCCATCGCCAACACCCGCATCGACTGGAAGGAGACCCCGGAGGCTCACATCTTCAAGGGGGACCTGCCGGGGATCAAAAAGGAAGAGGTGAAAGTGGAGGTGGAGGACGGGAGGGTGTTGCAGATCAGCGGGGAGAGGAGCCGGGAGCAGGAGGAGAAGAACGACACGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTTATGAGGAGGTTCAGGCTGCCGGAGAACGCGAAGCTTGATCAGGTGAAGGCGGCGATGGAGAACGGGGTGCTGACTGTAACTGTGCCGAAAGAGGTGAAGAGGCCTGATGTCAAGGCCATTGACATCTCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

270

Amino Acids

30.82

Weight (kDa)

9.38

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 166 - 267 2.1e-30 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 17, 41
Acc36I ACCTGC 1 cut(s) 546
Acc65I GGTACC 2 cut(s) 4, 241
AccB1I GGYRCC 2 cut(s) 4, 241
AccII CGCG 1 cut(s) 704
AciI CCGC 7 cut(s) 369, 459, 474, 490, 603, 666, 725
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 308
AcvI CACGTG 1 cut(s) 642
AdeI CACNNNGTG 1 cut(s) 652
AfaI GTAC 2 cut(s) 6, 243
AfiI CCNNNNNNNGG 4 cut(s) 86, 424, 425, 622
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 2 cut(s) 97, 529
AluBI AGCT 3 cut(s) 113, 158, 709
AluI AGCT 3 cut(s) 113, 158, 709
Alw26I GTCTC 2 cut(s) 365, 500
AlwI GGATC 1 cut(s) 554
AoxI GGCC 5 cut(s) 61, 433, 441, 776, 789
ApeKI GCWGC 3 cut(s) 158, 663, 691
ApoI RAATTY 1 cut(s) 308
Asp718I GGTACC 2 cut(s) 4, 241
AspS9I GGNCC 4 cut(s) 61, 415, 434, 535
AsuC2I CCSGG 3 cut(s) 512, 543, 617
AsuHPI GGTGA 3 cut(s) 574, 730, 781
AsuII TTCGAA 1 cut(s) 306
AvaII GGWCC 2 cut(s) 415, 535
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 2 cut(s) 4, 241
BbrPI CACGTG 1 cut(s) 642
BbvI GCAGC 3 cut(s) 145, 675, 678
BccI CCATC 4 cut(s) 110, 259, 485, 724
BceAI ACGGC 1 cut(s) 198
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 3 cut(s) 512, 543, 617
BcoDI GTCTC 2 cut(s) 365, 500
BfmI CTRYAG 2 cut(s) 159, 171
BfuAI ACCTGC 1 cut(s) 546
BglII AGATCT 1 cut(s) 286
BisI GCNGC 5 cut(s) 159, 664, 667, 692, 726
BlsI GCNGC 5 cut(s) 160, 665, 668, 693, 727
Bme1390I CCNGG 3 cut(s) 512, 543, 617
Bme18I GGWCC 2 cut(s) 415, 535
BmgT120I GGNCC 4 cut(s) 61, 415, 434, 535
BmiI GGNNCC 6 cut(s) 6, 243, 416, 417, 536, 614
BmrFI CCNGG 3 cut(s) 512, 543, 617
BmsI GCATC 2 cut(s) 352, 501
Bpu14I TTCGAA 1 cut(s) 306
BpuMI CCSGG 3 cut(s) 512, 543, 617
BsaAI YACGTR 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 510, 542
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 424, 425, 622
Bse1I ACTGG 1 cut(s) 503
BseDI CCNNGG 2 cut(s) 510, 542
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 4 cut(s) 86, 424, 425, 622
BseMII CTCAG 3 cut(s) 193, 209, 370
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 5 cut(s) 445, 625, 641, 673, 694
BseXI GCAGC 3 cut(s) 145, 675, 678
BseYI CCCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 5 cut(s) 63, 435, 443, 778, 791
BshNI GGYRCC 2 cut(s) 4, 241
BsiSI CCGG 7 cut(s) 239, 340, 512, 542, 616, 695, 804
BslFI GGGAC 2 cut(s) 428, 548
BslI CCNNNNNNNGG 4 cut(s) 86, 424, 425, 622
BsmAI GTCTC 2 cut(s) 365, 500
BsmFI GGGAC 2 cut(s) 428, 548
BsnI GGCC 5 cut(s) 63, 435, 443, 778, 791
Bso31I GGTCTC 1 cut(s) 500
Bsp119I TTCGAA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 286, 546, 597, 712
BspACI CCGC 7 cut(s) 369, 459, 474, 490, 603, 666, 725
BspANI GGCC 5 cut(s) 63, 435, 443, 778, 791
BspCNI CTCAG 3 cut(s) 192, 208, 369
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 6 cut(s) 6, 243, 416, 417, 536, 614
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 1 cut(s) 554
BspT104I TTCGAA 1 cut(s) 306
BspT107I GGYRCC 2 cut(s) 4, 241
BspTNI GGTCTC 1 cut(s) 500
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 2 cut(s) 510, 542
BssMI GATC 4 cut(s) 286, 546, 597, 712
Bst4CI ACNGT 2 cut(s) 751, 757
Bst6I CTCTTC 2 cut(s) 552, 767
BstBAI YACGTR 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 179, 195, 356
BstF5I GGATG 1 cut(s) 264
BstFNI CGCG 1 cut(s) 704
BstKTI GATC 4 cut(s) 289, 549, 600, 715
BstMAI GTCTC 2 cut(s) 365, 500
BstMBI GATC 4 cut(s) 286, 546, 597, 712
BstSCI CCNGG 3 cut(s) 510, 541, 615
BstSFI CTRYAG 2 cut(s) 159, 171
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 3 cut(s) 145, 675, 678
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuRI GGCC 5 cut(s) 63, 435, 443, 778, 791
BtgZI GCGATG 2 cut(s) 463, 743
BtsCI GGATG 1 cut(s) 264
BveI ACCTGC 1 cut(s) 546
Cfr13I GGNCC 4 cut(s) 61, 415, 434, 535
Csp6I GTAC 2 cut(s) 5, 242
CviQI GTAC 2 cut(s) 5, 242
DdeI CTNAG 3 cut(s) 179, 195, 356
DpnI GATC 4 cut(s) 288, 548, 599, 714
DpnII GATC 4 cut(s) 286, 546, 597, 712
DraIII CACNNNGTG 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 552, 767
EarI CTCTTC 2 cut(s) 552, 767
EciI GGCGGA 2 cut(s) 384, 448
Eco147I AGGCCT 1 cut(s) 778
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 415, 535
Eco72I CACGTG 1 cut(s) 642
EcoO109I RGGNCCY 2 cut(s) 415, 535
EcoRI GAATTC 1 cut(s) 308
FaiI YATR 9 cut(s) 17, 41, 54, 221, 223, 225, 227, 278, 677
FaqI GGGAC 2 cut(s) 428, 548
FauI CCCGC 2 cut(s) 497, 596
FbaI TGATCA 1 cut(s) 712
Fnu4HI GCNGC 5 cut(s) 159, 664, 667, 692, 726
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 5 cut(s) 159, 664, 667, 692, 726
GluI GCNGC 5 cut(s) 159, 664, 667, 692, 726
GsaI CCCAGC 1 cut(s) 321
HaeIII GGCC 5 cut(s) 63, 435, 443, 778, 791
HapII CCGG 7 cut(s) 239, 340, 512, 542, 616, 695, 804
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 2 cut(s) 31, 261
HpaII CCGG 7 cut(s) 239, 340, 512, 542, 616, 695, 804
HphI GGTGA 3 cut(s) 574, 730, 781
Hpy188I TCNGA 3 cut(s) 182, 198, 256
Hpy188III TCNNGA 3 cut(s) 97, 151, 404
Hpy99I CGWCG 1 cut(s) 378
HpyAV CCTTC 7 cut(s) 94, 157, 215, 313, 406, 496, 715
HpyCH4III ACNGT 2 cut(s) 751, 757
HpyCH4IV ACGT 1 cut(s) 641
HpyCH4V TGCA 2 cut(s) 161, 595
HpyF3I CTNAG 3 cut(s) 179, 195, 356
HpySE526I ACGT 1 cut(s) 641
KflI GGGWCCC 1 cut(s) 415
KpnI GGTACC 2 cut(s) 8, 245
Ksp22I TGATCA 1 cut(s) 712
Kzo9I GATC 4 cut(s) 286, 546, 597, 712
LmnI GCTCC 3 cut(s) 612, 619, 660
Lsp1109I GCAGC 3 cut(s) 145, 675, 678
LweI GCATC 2 cut(s) 352, 501
MaeII ACGT 1 cut(s) 641
MaeIII GTNAC 3 cut(s) 44, 232, 751
MalI GATC 4 cut(s) 288, 548, 599, 714
MboI GATC 4 cut(s) 286, 546, 597, 712
MboII GAAGA 5 cut(s) 379, 517, 569, 643, 784
MflI RGATCY 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 4 cut(s) 10, 24, 49, 308
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 1 cut(s) 40
Mox20I TGGCCA 1 cut(s) 443
MscI TGGCCA 1 cut(s) 443
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 2 cut(s) 603, 666
MspI CCGG 7 cut(s) 239, 340, 512, 542, 616, 695, 804
MspR9I CCNGG 3 cut(s) 512, 543, 617
MvnI CGCG 1 cut(s) 704
NciI CCSGG 3 cut(s) 512, 543, 617
NdeII GATC 4 cut(s) 286, 546, 597, 712
NlaIV GGNNCC 6 cut(s) 6, 243, 416, 417, 536, 614
NmuCI GTSAC 1 cut(s) 232
NspV TTCGAA 1 cut(s) 306
PceI AGGCCT 1 cut(s) 778
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 5 cut(s) 160, 665, 668, 693, 727
PleI GAGTC 1 cut(s) 39
PmaCI CACGTG 1 cut(s) 642
PmlI CACGTG 1 cut(s) 642
PpsI GAGTC 1 cut(s) 39
Ppu21I YACGTR 1 cut(s) 642
PpuMI RGGWCCY 2 cut(s) 415, 535
PsiI TTATAA 2 cut(s) 17, 41
Psp5II RGGWCCY 2 cut(s) 415, 535
PspCI CACGTG 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 317
PspN4I GGNNCC 6 cut(s) 6, 243, 416, 417, 536, 614
PspPI GGNCC 4 cut(s) 61, 415, 434, 535
PspPPI RGGWCCY 2 cut(s) 415, 535
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 2 cut(s) 6, 243
RsaNI GTAC 2 cut(s) 5, 242
SatI GCNGC 5 cut(s) 159, 664, 667, 692, 726
Sau3AI GATC 4 cut(s) 286, 546, 597, 712
Sau96I GGNCC 4 cut(s) 61, 415, 434, 535
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 3 cut(s) 512, 543, 617
SfaNI GCATC 2 cut(s) 352, 501
SfcI CTRYAG 2 cut(s) 159, 171
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 2 cut(s) 415, 535
Sse9I AATT 4 cut(s) 10, 24, 49, 308
SseBI AGGCCT 1 cut(s) 778
SsiI CCGC 7 cut(s) 369, 459, 474, 490, 603, 666, 725
StuI AGGCCT 1 cut(s) 778
StyD4I CCNGG 3 cut(s) 510, 541, 615
TaaI ACNGT 2 cut(s) 751, 757
TaiI ACGT 1 cut(s) 644
TaqI TCGA 4 cut(s) 306, 390, 423, 495
TasI AATT 4 cut(s) 10, 24, 49, 308
TauI GCSGC 2 cut(s) 669, 728
TfiI GAWTC 1 cut(s) 261
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 3 cut(s) 158, 663, 691
Tsp45I GTSAC 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 415, 535
XapI RAATTY 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.