pycom01g16380

kDa class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003719
Physical Location & Seq
Forward (+)
15972005 .. 15972472
468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16380.1

Sequence Viewer

Length: 468 bp
ATGGCTCTCAGTCTCTTTGGCAACGGCCGACAAAGCAACTTCTTCGACCCCTTCTCTCTGGAAATCTGGGATCCATTCGAGGGCTTCGGCACTCTGGCCAACATCCCCACCTCCGCCCGTGAAACCACCGCCATTGCCAACACCCGCATCGACTGGAAGGAGACACCGGAGGCCCACATCTTCAAGGCGGACCTCCCGGGGATAAAAAAGGAGGAGGTGAAAGTGGAGGTGGAGGACGGGAGGGTCCTGCAGATCAGCGGCGAGAGGAGCCGGGAGCAGGAGGAGAAGAACGACAAGTGGCACAGGGTGGAGAGGAGCAGTGGCAAGTTTATGAGGAGGTTCAGGCTGCCGGAGAACGCGAAGATCGATCAGGTGAAGGCATCGATGGAGAATGGAGTCCTGACTGTGACTGTGCCGAAAGAGGAGGAGAAGAAGCCTGCTGTCAAGGCCATTGACATCTCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

156

Amino Acids

17.6

Weight (kDa)

5.83

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 154 7.8e-33 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccII CGCG 1 cut(s) 359
AciI CCGC 5 cut(s) 114, 129, 145, 188, 258
AclWI GGATC 2 cut(s) 65, 78
AcoI YGGCCR 2 cut(s) 25, 96
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 80, 277
AgsI TTSAA 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 17, 155
AlwI GGATC 2 cut(s) 65, 78
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 4 cut(s) 25, 96, 171, 447
ApeKI GCWGC 1 cut(s) 346
AspS9I GGNCC 3 cut(s) 172, 190, 244
AsuC2I CCSGG 3 cut(s) 197, 198, 272
AsuHPI GGTGA 2 cut(s) 229, 385
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 2 cut(s) 190, 244
BalI TGGCCA 1 cut(s) 98
BamHI GGATCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 379
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BcnI CCSGG 3 cut(s) 197, 198, 272
BcoDI GTCTC 2 cut(s) 17, 155
BfmI CTRYAG 1 cut(s) 248
BisI GCNGC 2 cut(s) 259, 347
BlsI GCNGC 2 cut(s) 260, 348
Bme1390I CCNGG 3 cut(s) 197, 198, 272
Bme18I GGWCC 2 cut(s) 190, 244
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 172, 190, 244
BmiI GGNNCC 3 cut(s) 72, 245, 269
BmrFI CCNGG 3 cut(s) 197, 198, 272
BmsI GCATC 2 cut(s) 156, 389
BpuMI CCSGG 3 cut(s) 197, 198, 272
Bsa29I ATCGAT 2 cut(s) 366, 383
BsaJI CCNNGG 2 cut(s) 196, 197
BsaWI WCCGGW 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 277
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 132
BseCI ATCGAT 2 cut(s) 366, 383
BseDI CCNNGG 2 cut(s) 196, 197
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 80, 277
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 7 cut(s) 227, 280, 296, 328, 349, 437, 440
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 4 cut(s) 27, 98, 173, 449
BshVI ATCGAT 2 cut(s) 366, 383
BsiEI CGRYCG 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 5 cut(s) 167, 197, 271, 350, 462
BslI CCNNNNNNNGG 2 cut(s) 80, 277
BsmAI GTCTC 2 cut(s) 17, 155
BsnI GGCC 4 cut(s) 27, 98, 173, 449
BsoBI CYCGRG 1 cut(s) 196
Bsp143I GATC 4 cut(s) 70, 252, 363, 367
BspACI CCGC 5 cut(s) 114, 129, 145, 188, 258
BspANI GGCC 4 cut(s) 27, 98, 173, 449
BspCNI CTCAG 1 cut(s) 21
BspDI ATCGAT 2 cut(s) 366, 383
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 3 cut(s) 72, 245, 269
BspMAI CTGCAG 1 cut(s) 252
BspPI GGATC 2 cut(s) 65, 78
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 196, 197
BssMI GATC 4 cut(s) 70, 252, 363, 367
Bst4CI ACNGT 2 cut(s) 406, 412
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 4 cut(s) 73, 255, 366, 370
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 4 cut(s) 70, 252, 363, 367
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 3 cut(s) 33, 267, 446
BstSCI CCNGG 3 cut(s) 195, 196, 270
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BstZI CGGCCG 1 cut(s) 25
Bsu15I ATCGAT 2 cut(s) 366, 383
BsuRI GGCC 4 cut(s) 27, 98, 173, 449
BsuTUI ATCGAT 2 cut(s) 366, 383
BtsCI GGATG 1 cut(s) 102
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 438
Cfr13I GGNCC 3 cut(s) 172, 190, 244
Cfr9I CCCGGG 1 cut(s) 196
ClaI ATCGAT 2 cut(s) 366, 383
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
DdeI CTNAG 1 cut(s) 8
DpnI GATC 4 cut(s) 72, 254, 365, 369
DpnII GATC 4 cut(s) 70, 252, 363, 367
DraIII CACNNNGTG 1 cut(s) 307
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 25, 96
EagI CGGCCG 1 cut(s) 25
EciI GGCGGA 2 cut(s) 103, 203
EclXI CGGCCG 1 cut(s) 25
Eco47I GGWCC 2 cut(s) 190, 244
Eco52I CGGCCG 1 cut(s) 25
Eco88I CYCGRG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 244
FaiI YATR 1 cut(s) 332
FauI CCCGC 1 cut(s) 152
Fnu4HI GCNGC 2 cut(s) 259, 347
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 259, 347
GluI GCNGC 2 cut(s) 259, 347
HaeIII GGCC 4 cut(s) 27, 98, 173, 449
HapII CCGG 5 cut(s) 167, 197, 271, 350, 462
HinfI GANTC 1 cut(s) 396
HpaII CCGG 5 cut(s) 167, 197, 271, 350, 462
HphI GGTGA 2 cut(s) 229, 385
Hpy188III TCNNGA 2 cut(s) 59, 400
HpyAV CCTTC 3 cut(s) 61, 151, 370
HpyCH4III ACNGT 2 cut(s) 406, 412
HpyCH4V TGCA 1 cut(s) 250
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 267, 446
HpyF3I CTNAG 1 cut(s) 8
Kzo9I GATC 4 cut(s) 70, 252, 363, 367
LmnI GCTCC 3 cut(s) 267, 274, 315
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 156, 389
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 72, 254, 365, 369
MboI GATC 4 cut(s) 70, 252, 363, 367
MboII GAAGA 5 cut(s) 34, 172, 298, 373, 442
MflI RGATCY 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 405
Mox20I TGGCCA 1 cut(s) 98
MscI TGGCCA 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 5 cut(s) 167, 197, 271, 350, 462
MspR9I CCNGG 3 cut(s) 197, 198, 272
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 33, 267, 446
NciI CCSGG 3 cut(s) 197, 198, 272
NdeII GATC 4 cut(s) 70, 252, 363, 367
NlaIV GGNNCC 3 cut(s) 72, 245, 269
NmuCI GTSAC 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PkrI GCNGC 2 cut(s) 260, 348
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 72, 245, 269
PspPI GGNCC 3 cut(s) 172, 190, 244
PspPPI RGGWCCY 1 cut(s) 244
PstI CTGCAG 1 cut(s) 252
PsuI RGATCY 1 cut(s) 70
SatI GCNGC 2 cut(s) 259, 347
Sau3AI GATC 4 cut(s) 70, 252, 363, 367
Sau96I GGNCC 3 cut(s) 172, 190, 244
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 3 cut(s) 197, 198, 272
SetI ASST 6 cut(s) 113, 195, 219, 231, 341, 375
SfaNI GCATC 2 cut(s) 156, 389
SfcI CTRYAG 1 cut(s) 248
SinI GGWCC 2 cut(s) 190, 244
SmaI CCCGGG 1 cut(s) 198
SsiI CCGC 5 cut(s) 114, 129, 145, 188, 258
StyD4I CCNGG 3 cut(s) 195, 196, 270
TaaI ACNGT 2 cut(s) 406, 412
TaqI TCGA 5 cut(s) 45, 78, 150, 366, 383
TauI GCSGC 1 cut(s) 261
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 406
TspMI CCCGGG 1 cut(s) 196
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 2 cut(s) 190, 244
XmaI CCCGGG 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.