MD07G1210400.v1.1

kDa class I heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003719
Physical Location & Seq
Forward (+)
28942672 .. 28943139
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1210400.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCTCTCAGTCTCTTTGGAAACGGCCGACGAAGCAACGTTTTCGACCCCTTCTCTCTCGACATCTGGGACCCGTTCGAGGGCTTCAGCACTCTGGCCAACATCCCCTCCTCCGCCCGGGAAACAACCGCCATCGCCAACACCCACATCGACTGGAAGGAGACCCCGGAGGCCCACATCTTCAAGGCGGACCTCCCGGGGATCAAAAAGGAGGAGGTGAAAGTGGAGGTGGAGGACGGGAGGGTCCTCCAGATCAGCGGCGAGAGGAGCCGGGAGCAGGAGGATAAGAACGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTCGTAAGGAGGTTCAGGCTGCCGGAGAACGCGAAGATTGATCAGGTGAAGGCGGCGATGGAGAATGGGGTGCTGACTGTGACTGTGCCGAAAGAGGTGGAGAAGAAGCCTGAGGTGAAGGCCATTGACATTTCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

156

Amino Acids

17.53

Weight (kDa)

5.99

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 154 6.5e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccII CGCG 1 cut(s) 359
AciI CCGC 6 cut(s) 114, 129, 188, 258, 321, 380
AclI AACGTT 1 cut(s) 39
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 2 cut(s) 25, 96
AcuI CTGAAG 1 cut(s) 70
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 4 cut(s) 79, 80, 117, 277
AgsI TTSAA 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 17, 155
AlwI GGATC 1 cut(s) 209
Ama87I CYCGRG 2 cut(s) 117, 196
AoxI GGCC 4 cut(s) 25, 96, 171, 447
ApeKI GCWGC 2 cut(s) 318, 346
AspS9I GGNCC 4 cut(s) 70, 172, 190, 244
AsuC2I CCSGG 6 cut(s) 118, 119, 167, 197, 198, 272
AsuHPI GGTGA 3 cut(s) 229, 385, 454
AvaI CYCGRG 2 cut(s) 117, 196
AvaII GGWCC 3 cut(s) 70, 190, 244
AxyI CCTNAGG 1 cut(s) 438
BalI TGGCCA 1 cut(s) 98
BbvI GCAGC 2 cut(s) 330, 333
BccI CCATC 2 cut(s) 140, 379
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 6 cut(s) 118, 119, 167, 197, 198, 272
BcoDI GTCTC 2 cut(s) 17, 155
BisI GCNGC 5 cut(s) 259, 319, 322, 347, 381
BlsI GCNGC 5 cut(s) 260, 320, 323, 348, 382
Bme1390I CCNGG 6 cut(s) 118, 119, 167, 197, 198, 272
Bme18I GGWCC 3 cut(s) 70, 190, 244
BmeT110I CYCGRG 2 cut(s) 117, 196
BmgT120I GGNCC 4 cut(s) 70, 172, 190, 244
BmiI GGNNCC 4 cut(s) 71, 72, 245, 269
BmrFI CCNGG 6 cut(s) 118, 119, 167, 197, 198, 272
BpmI CTGGAG 1 cut(s) 233
BpuMI CCSGG 6 cut(s) 118, 119, 167, 197, 198, 272
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 4 cut(s) 117, 165, 196, 197
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 80, 117, 277
Bse1I ACTGG 1 cut(s) 158
Bse21I CCTNAGG 1 cut(s) 438
BseDI CCNNGG 4 cut(s) 117, 165, 196, 197
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 4 cut(s) 79, 80, 117, 277
BseMII CTCAG 2 cut(s) 22, 429
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 4 cut(s) 100, 227, 280, 328
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 330, 333
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 4 cut(s) 27, 98, 173, 449
BsiEI CGRYCG 1 cut(s) 28
BsiHKCI CYCGRG 2 cut(s) 117, 196
BsiSI CCGG 6 cut(s) 118, 167, 197, 271, 350, 462
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 4 cut(s) 79, 80, 117, 277
BsmAI GTCTC 2 cut(s) 17, 155
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 4 cut(s) 27, 98, 173, 449
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 2 cut(s) 117, 196
Bsp143I GATC 3 cut(s) 201, 252, 367
BspACI CCGC 6 cut(s) 114, 129, 188, 258, 321, 380
BspANI GGCC 4 cut(s) 27, 98, 173, 449
BspCNI CTCAG 2 cut(s) 21, 430
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 71, 72, 245, 269
BspPI GGATC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 4 cut(s) 117, 165, 196, 197
BssMI GATC 3 cut(s) 201, 252, 367
Bst4CI ACNGT 2 cut(s) 406, 412
BstDEI CTNAG 2 cut(s) 8, 438
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 3 cut(s) 204, 255, 370
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 3 cut(s) 201, 252, 367
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 33, 267
BstSCI CCNGG 6 cut(s) 116, 117, 165, 195, 196, 270
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 330, 333
BstZI CGGCCG 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 438
BsuRI GGCC 4 cut(s) 27, 98, 173, 449
BtgZI GCGATG 2 cut(s) 118, 398
BtsCI GGATG 1 cut(s) 102
Cfr13I GGNCC 4 cut(s) 70, 172, 190, 244
Cfr9I CCCGGG 2 cut(s) 117, 196
DdeI CTNAG 2 cut(s) 8, 438
DpnI GATC 3 cut(s) 203, 254, 369
DpnII GATC 3 cut(s) 201, 252, 367
DraIII CACNNNGTG 1 cut(s) 307
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 25, 96
EagI CGGCCG 1 cut(s) 25
EciI GGCGGA 2 cut(s) 103, 203
EclXI CGGCCG 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 3 cut(s) 70, 190, 244
Eco52I CGGCCG 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 70
Eco81I CCTNAGG 1 cut(s) 438
Eco88I CYCGRG 2 cut(s) 117, 196
EcoO109I RGGNCCY 2 cut(s) 70, 244
FaqI GGGAC 1 cut(s) 83
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 5 cut(s) 259, 319, 322, 347, 381
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 5 cut(s) 259, 319, 322, 347, 381
GluI GCNGC 5 cut(s) 259, 319, 322, 347, 381
GsuI CTGGAG 1 cut(s) 233
HaeIII GGCC 4 cut(s) 27, 98, 173, 449
HapII CCGG 6 cut(s) 118, 167, 197, 271, 350, 462
HpaII CCGG 6 cut(s) 118, 167, 197, 271, 350, 462
HphI GGTGA 3 cut(s) 229, 385, 454
Hpy188III TCNNGA 2 cut(s) 59, 250
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 4 cut(s) 61, 151, 370, 439
HpyCH4III ACNGT 2 cut(s) 406, 412
HpyCH4IV ACGT 1 cut(s) 39
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 267
HpyF3I CTNAG 2 cut(s) 8, 438
HpySE526I ACGT 1 cut(s) 39
KflI GGGWCCC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 3 cut(s) 201, 252, 367
LmnI GCTCC 3 cut(s) 267, 274, 315
Lsp1109I GCAGC 2 cut(s) 330, 333
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 406
MalI GATC 3 cut(s) 203, 254, 369
MboI GATC 3 cut(s) 201, 252, 367
MboII GAAGA 3 cut(s) 172, 373, 442
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
Mox20I TGGCCA 1 cut(s) 98
MscI TGGCCA 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 2 cut(s) 258, 321
MspI CCGG 6 cut(s) 118, 167, 197, 271, 350, 462
MspR9I CCNGG 6 cut(s) 118, 119, 167, 197, 198, 272
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 33, 267
NciI CCSGG 6 cut(s) 118, 119, 167, 197, 198, 272
NdeII GATC 3 cut(s) 201, 252, 367
NlaIV GGNNCC 4 cut(s) 71, 72, 245, 269
NmuCI GTSAC 1 cut(s) 406
PkrI GCNGC 5 cut(s) 260, 320, 323, 348, 382
PpuMI RGGWCCY 2 cut(s) 70, 244
Psp1406I AACGTT 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 70, 244
PspN4I GGNNCC 4 cut(s) 71, 72, 245, 269
PspPI GGNCC 4 cut(s) 70, 172, 190, 244
PspPPI RGGWCCY 2 cut(s) 70, 244
SatI GCNGC 5 cut(s) 259, 319, 322, 347, 381
Sau3AI GATC 3 cut(s) 201, 252, 367
Sau96I GGNCC 4 cut(s) 70, 172, 190, 244
ScrFI CCNGG 6 cut(s) 118, 119, 167, 197, 198, 272
SetI ASST 8 cut(s) 42, 195, 219, 231, 341, 375, 426, 444
SinI GGWCC 3 cut(s) 70, 190, 244
SmaI CCCGGG 2 cut(s) 119, 198
SsiI CCGC 6 cut(s) 114, 129, 188, 258, 321, 380
StyD4I CCNGG 6 cut(s) 116, 117, 165, 195, 196, 270
TaaI ACNGT 2 cut(s) 406, 412
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 45, 60, 78, 150
TauI GCSGC 3 cut(s) 261, 324, 383
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 2 cut(s) 318, 346
Tsp45I GTSAC 1 cut(s) 406
TspMI CCCGGG 2 cut(s) 117, 196
VpaK11BI GGWCC 3 cut(s) 70, 190, 244
XmaI CCCGGG 2 cut(s) 117, 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.