AT3G46230

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003719
Physical Location & Seq
Reverse (-)
16984118 .. 16984867
750 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G46230.1

Sequence Viewer

Length: 471 bp
ATGTCTCTAGTTCCGAGCTTTTTTGGAGGCCGAAGAACAAACGTGTTCGACCCATTTTCACTAGACGTATGGGATCCGTTCGAAGGATTCTTGACGCCCGGGTTGACAAACGCACCTGCCAAGGACGTGGCAGCGTTCACAAACGCTAAAGTGGACTGGAGGGAGACACCTGAAGCGCATGTGTTCAAGGCGGACGTGCCTGGGCTTAAGAAGGAAGAGGTGAAGGTGGAGGTTGAAGATGGTAACATACTTCAGATAAGCGGTGAGAGGAGCAGTGAGAATGAAGAGAAGAGTGACACATGGCACCGTGTGGAGCGGTCAAGTGGGAAGTTCATGAGGAGGTTTCGGTTGCCAGAGAATGCAAAGGTGGAGGAAGTAAAGGCGAGTATGGAGAATGGGGTGTTGTCGGTTACGGTGCCGAAAGTTCAGGAGAGTAAGCCGGAGGTCAAGTCCGTTGATATCTCTGGTTAA

Protein Analysis

156

Amino Acids

17.44

Weight (kDa)

5.2

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 52 - 155 2.6e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 124
Acc36I ACCTGC 1 cut(s) 124
AccB1I GGYRCC 2 cut(s) 303, 415
AccBSI CCGCTC 1 cut(s) 316
AciI CCGC 3 cut(s) 191, 261, 316
AclWI GGATC 2 cut(s) 68, 81
AcuI CTGAAG 2 cut(s) 192, 236
AcyI GRCGYC 1 cut(s) 95
AdeI CACNNNGTG 1 cut(s) 310
AfiI CCNNNNNNNGG 1 cut(s) 83
AflII CTTAAG 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 2 cut(s) 187, 236
AjiI CACGTC 2 cut(s) 127, 196
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 1 cut(s) 18
AluI AGCT 1 cut(s) 18
Alw26I GTCTC 2 cut(s) 9, 158
AlwI GGATC 2 cut(s) 68, 81
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 178
AsuC2I CCSGG 2 cut(s) 99, 100
AsuHPI GGTGA 2 cut(s) 232, 275
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 98
BamHI GGATCC 1 cut(s) 73
BanI GGYRCC 2 cut(s) 303, 415
BbvI GCAGC 1 cut(s) 143
BccI CCATC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 201
BcnI CCSGG 2 cut(s) 99, 100
BcoDI GTCTC 2 cut(s) 9, 158
BfaI CTAG 2 cut(s) 8, 62
BfrI CTTAAG 1 cut(s) 206
BfuAI ACCTGC 1 cut(s) 124
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 3 cut(s) 99, 100, 201
BmeT110I CYCGRG 1 cut(s) 98
BmgBI CACGTC 2 cut(s) 127, 196
BmiI GGNNCC 3 cut(s) 75, 305, 417
BmrFI CCNGG 3 cut(s) 99, 100, 201
BpmI CTGGAG 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 81
BpuMI CCSGG 2 cut(s) 99, 100
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 98, 120, 200
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 98, 120, 200
BseLI CCNNNNNNNGG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 2 cut(s) 283, 352
BseXI GCAGC 1 cut(s) 143
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 2 cut(s) 303, 415
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 99, 440
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 9, 158
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 81
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 3 cut(s) 191, 261, 316
BspANI GGCC 1 cut(s) 30
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 75, 305, 417
BspMI ACCTGC 1 cut(s) 124
BspPI GGATC 2 cut(s) 68, 81
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 303, 415
BspTI CTTAAG 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 316
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 3 cut(s) 98, 120, 200
BssMI GATC 1 cut(s) 73
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 2 cut(s) 308, 415
Bst6I CTCTTC 3 cut(s) 210, 279, 284
BstACI GRCGYC 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 206
BstBI TTCGAA 1 cut(s) 81
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 2 cut(s) 9, 158
BstMBI GATC 1 cut(s) 73
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 3 cut(s) 97, 98, 199
BstV1I GCAGC 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 1 cut(s) 30
BtrI CACGTC 2 cut(s) 127, 196
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 280
BveI ACCTGC 1 cut(s) 124
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 178
Cfr9I CCCGGG 1 cut(s) 98
CseI GACGC 1 cut(s) 103
CviAII CATG 3 cut(s) 179, 300, 334
CviJI RGCY 4 cut(s) 18, 30, 205, 439
CviKI_1 RGCY 4 cut(s) 18, 30, 205, 439
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 210, 279, 284
EarI CTCTTC 3 cut(s) 210, 279, 284
EciI GGCGGA 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 120
Eco32I GATATC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 192, 236
Eco88I CYCGRG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 199
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 3 cut(s) 182, 303, 337
FaiI YATR 6 cut(s) 70, 180, 248, 301, 335, 389
FatI CATG 3 cut(s) 178, 299, 333
Fnu4HI GCNGC 1 cut(s) 132
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 2 cut(s) 8, 62
GlaI GCGC 1 cut(s) 177
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 99, 440
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 182, 303, 337
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 1 cut(s) 87
HpaII CCGG 2 cut(s) 99, 440
HphI GGTGA 2 cut(s) 232, 275
Hpy166II GTNNAC 3 cut(s) 105, 138, 154
Hpy188I TCNGA 2 cut(s) 15, 255
Hpy188III TCNNGA 3 cut(s) 91, 334, 428
Hpy8I GTNNAC 3 cut(s) 105, 138, 154
HpyAV CCTTC 3 cut(s) 77, 205, 217
HpyCH4III ACNGT 2 cut(s) 308, 415
HpyCH4IV ACGT 4 cut(s) 42, 66, 126, 195
HpyCH4V TGCA 1 cut(s) 362
HpySE526I ACGT 4 cut(s) 42, 66, 126, 195
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 182, 303, 337
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 2 cut(s) 270, 313
Lsp1109I GCAGC 1 cut(s) 143
MaeI CTAG 2 cut(s) 8, 62
MaeII ACGT 4 cut(s) 42, 66, 126, 195
MaeIII GTNAC 3 cut(s) 242, 293, 409
MalI GATC 1 cut(s) 75
MbiI CCGCTC 1 cut(s) 316
MboI GATC 1 cut(s) 73
MboII GAAGA 5 cut(s) 45, 227, 248, 296, 301
MflI RGATCY 1 cut(s) 73
MnlI CCTC 9 cut(s) 20, 153, 211, 223, 261, 330, 333, 364, 436
MseI TTAA 2 cut(s) 207, 469
MspCI CTTAAG 1 cut(s) 206
MspI CCGG 2 cut(s) 99, 440
MspR9I CCNGG 3 cut(s) 99, 100, 201
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 201
NciI CCSGG 2 cut(s) 99, 100
NdeII GATC 1 cut(s) 73
NlaIII CATG 3 cut(s) 182, 303, 337
NlaIV GGNNCC 3 cut(s) 75, 305, 417
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 182
NspV TTCGAA 1 cut(s) 81
PagI TCATGA 1 cut(s) 333
PaqCI CACCTGC 1 cut(s) 124
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 3 cut(s) 75, 305, 417
PsuI RGATCY 1 cut(s) 73
SaqAI TTAA 2 cut(s) 207, 469
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 1 cut(s) 73
ScrFI CCNGG 3 cut(s) 99, 100, 201
SfuI TTCGAA 1 cut(s) 81
SmaI CCCGGG 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SsiI CCGC 3 cut(s) 191, 261, 316
SspMI CTAG 2 cut(s) 8, 62
StyD4I CCNGG 3 cut(s) 97, 98, 199
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 308, 415
TaiI ACGT 4 cut(s) 45, 69, 129, 198
TaqI TCGA 2 cut(s) 48, 81
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 2 cut(s) 207, 469
Tru9I TTAA 2 cut(s) 207, 469
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 297, 322
TspGWI ACGGA 2 cut(s) 66, 442
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 1 cut(s) 280
Vha464I CTTAAG 1 cut(s) 206
XceI RCATGY 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 98
XspI CTAG 2 cut(s) 8, 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.