RLG00000027166

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003719
Physical Location & Seq
Reverse (-)
9203195 .. 9204235
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027166

Sequence Viewer

Length: 591 bp
ATGGAGGAGCACAAGATTGCTGGGTCTTCAATCTCATCAAACAAGAGTCATCCAAAGCAACCAAGTCTTCCAATACCTACCTACACCAGCAAATCAAAAACCTCAGCAAACCCATCAGCAATGGCTCTCAGTCTCTTTGGCACCGGCCGACGCAGCAACGTCTTCGACCCATTTTCCCTCGACATCTGGGACCCCTTCCAGGGCATAGGCTCTCTCGTCAACTCCTCCTCCACTGCCGGCGACACCTCCGCCTTCGCTCAGACCCGAATCGACTGGAAGGAGACCCCGGAGGCCCACATCTTCAAGGCGGACCTCCCCGGACTGAAGAAGGAAGAAGTGAAGGTTGAGCTGGAGGAAGGGAATGTCCTTCAGATCAGCGGAGAGAGGAGCAGGGAGCAGGAGGAGAAGAATGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTCCTGAGGAGGTTCAGGCTGCCGGATAATGCAAAGGTGGATCATGTCAGGGCTTCTATGGAGAATGGGGTTCTGACTGTGACTGTCCCCAAAGCAGAGGAGCAGAAGCCCCAGGTCAAGAGCATCGACATTTCTGGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

197

Amino Acids

21.69

Weight (kDa)

7.95

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 91 - 194 4.1e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 140
AciI CCGC 4 cut(s) 249, 308, 378, 441
AclWI GGATC 1 cut(s) 495
AcoI YGGCCR 1 cut(s) 145
AcuI CTGAAG 2 cut(s) 344, 353
AdeI CACNNNGTG 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 199, 200
AgsI TTSAA 2 cut(s) 30, 304
AjnI CCWGG 2 cut(s) 198, 558
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 2 cut(s) 137, 275
AlwI GGATC 1 cut(s) 495
AoxI GGCC 2 cut(s) 145, 291
ApeKI GCWGC 3 cut(s) 153, 438, 466
AspS9I GGNCC 3 cut(s) 190, 292, 310
AsuC2I CCSGG 2 cut(s) 287, 318
AvaII GGWCC 2 cut(s) 190, 310
AxyI CCTNAGG 1 cut(s) 452
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 18, 59, 154
Bbv12I GWGCWC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 165, 450, 453
BccI CCATC 1 cut(s) 121
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 2 cut(s) 200, 560
BcnI CCSGG 2 cut(s) 287, 318
BcoDI GTCTC 2 cut(s) 137, 275
BisI GCNGC 4 cut(s) 154, 439, 442, 467
BlsI GCNGC 4 cut(s) 155, 440, 443, 468
Bme1390I CCNGG 4 cut(s) 200, 287, 318, 560
Bme18I GGWCC 2 cut(s) 190, 310
BmgT120I GGNCC 3 cut(s) 190, 292, 310
BmiI GGNNCC 4 cut(s) 142, 191, 192, 586
BmrFI CCNGG 4 cut(s) 200, 287, 318, 560
BmsI GCATC 1 cut(s) 579
BpiI GAAGAC 3 cut(s) 18, 59, 154
BpmI CTGGAG 1 cut(s) 371
Bpu10I CCTNAGC 1 cut(s) 103
BpuMI CCSGG 2 cut(s) 287, 318
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 199, 285, 316, 558
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 200
Bse118I RCCGGY 2 cut(s) 143, 236
Bse1I ACTGG 1 cut(s) 278
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 2 cut(s) 200, 560
BseDI CCNNGG 4 cut(s) 199, 285, 316, 558
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 2 cut(s) 199, 200
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 4 cut(s) 117, 142, 272, 443
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 8 cut(s) 20, 214, 217, 400, 416, 448, 469, 560
BseX3I CGGCCG 1 cut(s) 145
BseXI GCAGC 3 cut(s) 165, 450, 453
BseYI CCCAGC 1 cut(s) 20
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 2 cut(s) 147, 293
BshNI GGYRCC 1 cut(s) 140
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 5 cut(s) 144, 237, 287, 318, 470
BslFI GGGAC 2 cut(s) 203, 518
BslI CCNNNNNNNGG 2 cut(s) 199, 200
BsmAI GTCTC 2 cut(s) 137, 275
BsmFI GGGAC 2 cut(s) 203, 518
BsnI GGCC 2 cut(s) 147, 293
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 12
Bsp143I GATC 2 cut(s) 372, 487
BspACI CCGC 4 cut(s) 249, 308, 378, 441
BspANI GGCC 2 cut(s) 147, 293
BspCNI CTCAG 4 cut(s) 116, 141, 271, 444
BspLI GGNNCC 4 cut(s) 142, 191, 192, 586
BspPI GGATC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 2 cut(s) 143, 236
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 2 cut(s) 143, 236
BssECI CCNNGG 4 cut(s) 199, 285, 316, 558
BssMI GATC 2 cut(s) 372, 487
Bst2UI CCWGG 2 cut(s) 200, 560
Bst4CI ACNGT 2 cut(s) 526, 532
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 4 cut(s) 103, 128, 258, 452
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 2 cut(s) 375, 490
BstMAI GTCTC 2 cut(s) 137, 275
BstMBI GATC 2 cut(s) 372, 487
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstNI CCWGG 2 cut(s) 200, 560
BstSCI CCNGG 4 cut(s) 198, 285, 316, 558
BstV1I GCAGC 3 cut(s) 165, 450, 453
BstV2I GAAGAC 3 cut(s) 18, 59, 154
BstZI CGGCCG 1 cut(s) 145
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 2 cut(s) 147, 293
BtsCI GGATG 1 cut(s) 49
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 238
Cfr10I RCCGGY 2 cut(s) 143, 236
Cfr13I GGNCC 3 cut(s) 190, 292, 310
CseI GACGC 1 cut(s) 159
CviAII CATG 1 cut(s) 491
CviJI RGCY 9 cut(s) 125, 147, 210, 293, 349, 466, 500, 556, 587
CviKI_1 RGCY 9 cut(s) 125, 147, 210, 293, 349, 466, 500, 556, 587
DdeI CTNAG 4 cut(s) 103, 128, 258, 452
DpnI GATC 2 cut(s) 374, 489
DpnII GATC 2 cut(s) 372, 487
DraIII CACNNNGTG 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 145
EagI CGGCCG 1 cut(s) 145
EciI GGCGGA 2 cut(s) 238, 323
EclXI CGGCCG 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 190, 310
Eco52I CGGCCG 1 cut(s) 145
Eco57I CTGAAG 2 cut(s) 344, 353
Eco81I CCTNAGG 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 198, 558
FaeI CATG 1 cut(s) 494
FaiI YATR 3 cut(s) 206, 492, 506
FaqI GGGAC 2 cut(s) 203, 518
FatI CATG 1 cut(s) 490
Fnu4HI GCNGC 4 cut(s) 154, 439, 442, 467
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 4 cut(s) 154, 439, 442, 467
GluI GCNGC 4 cut(s) 154, 439, 442, 467
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 371
HaeIII GGCC 2 cut(s) 147, 293
HapII CCGG 5 cut(s) 144, 237, 287, 318, 470
HgaI GACGC 1 cut(s) 159
Hin1II CATG 1 cut(s) 494
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 46, 267
HpaII CCGG 5 cut(s) 144, 237, 287, 318, 470
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 3 cut(s) 261, 372, 522
Hpy188III TCNNGA 3 cut(s) 451, 565, 582
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 7 cut(s) 205, 262, 271, 322, 334, 350, 377
HpyCH4III ACNGT 2 cut(s) 526, 532
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 1 cut(s) 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 4 cut(s) 103, 128, 258, 452
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 1 cut(s) 494
KflI GGGWCCC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 2 cut(s) 372, 487
LmnI GCTCC 6 cut(s) 7, 387, 394, 435, 547, 584
Lsp1109I GCAGC 3 cut(s) 165, 450, 453
LweI GCATC 1 cut(s) 579
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 526
MalI GATC 2 cut(s) 374, 489
MboI GATC 2 cut(s) 372, 487
MboII GAAGA 7 cut(s) 18, 59, 154, 292, 337, 344, 418
MhlI GDGCHC 1 cut(s) 12
MlyI GAGTC 1 cut(s) 55
MroNI GCCGGC 1 cut(s) 236
MspA1I CMGCKG 2 cut(s) 378, 441
MspI CCGG 5 cut(s) 144, 237, 287, 318, 470
MspR9I CCNGG 4 cut(s) 200, 287, 318, 560
MvaI CCWGG 2 cut(s) 200, 560
MwoI GCNNNNNNNGC 1 cut(s) 153
NaeI GCCGGC 1 cut(s) 238
NciI CCSGG 2 cut(s) 287, 318
NdeII GATC 2 cut(s) 372, 487
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 1 cut(s) 494
NlaIV GGNNCC 4 cut(s) 142, 191, 192, 586
NmuCI GTSAC 1 cut(s) 526
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 267
PkrI GCNGC 4 cut(s) 155, 440, 443, 468
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 198, 558
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 2 cut(s) 198, 558
PspN4I GGNNCC 4 cut(s) 142, 191, 192, 586
PspPI GGNCC 3 cut(s) 190, 292, 310
PspPPI RGGWCCY 1 cut(s) 190
SatI GCNGC 4 cut(s) 154, 439, 442, 467
Sau3AI GATC 2 cut(s) 372, 487
Sau96I GGNCC 3 cut(s) 190, 292, 310
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 4 cut(s) 200, 287, 318, 560
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 1 cut(s) 579
SinI GGWCC 2 cut(s) 190, 310
SsiI CCGC 4 cut(s) 249, 308, 378, 441
StyD4I CCNGG 4 cut(s) 198, 285, 316, 558
TaaI ACNGT 2 cut(s) 526, 532
TaiI ACGT 1 cut(s) 162
TaqI TCGA 4 cut(s) 165, 180, 270, 573
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 1 cut(s) 267
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 3 cut(s) 153, 438, 466
Tsp45I GTSAC 1 cut(s) 526
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 190, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.