FvH4_7g23710

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003719
Physical Location & Seq
Forward (+)
18441951 .. 18447642
5692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23710.t1

Sequence Viewer

Length: 411 bp
ATGGCTCTAAGTCTCTTTGGCAATGGCCGACGCAGCAACGTCTTCGACCCATTTTCCCTCGACATCTGGGACCCCTTCCAGGGATTTGGCCCTCTCATGAACTCCTCCTCCACCGCCGGCGAAACCTCCGCCTTCGCGCAGACCCGAATCGACTGGAAGGAGACTCCGGAGGCTCATGTCTTCAAGGCGGACCTGCCCGGACTGAAGAAGGAAGAAGTGAAGGTTGAGCTGGAGGAAGGGAATGTCCTTCAGATCAGCGGAGAGAGGAGCAAGGAGCAGGAGGAGAAGAACGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTCATGAGGAGGTTCAGGCTGCCGGACAATGCAAAGGTGGATCAGGTTCAGGCTGCCGGACAATGCAAAGGTGGATCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0022607 GO:0043933 GO:0044085 GO:0050896 GO:0051259 GO:0065003 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

137

Amino Acids

15.23

Weight (kDa)

6.15

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 128 3.9e-22 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 201
AccII CGCG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 166
AciI CCGC 5 cut(s) 114, 129, 188, 258, 321
AclWI GGATC 2 cut(s) 375, 409
AcoI YGGCCR 1 cut(s) 25
AcuI CTGAAG 2 cut(s) 224, 233
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 79, 80
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 78
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 17, 155
AlwI GGATC 2 cut(s) 375, 409
Aor13HI TCCGGA 1 cut(s) 166
AoxI GGCC 2 cut(s) 25, 88
ApeKI GCWGC 4 cut(s) 33, 318, 346, 380
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 3 cut(s) 70, 89, 190
AsuC2I CCSGG 1 cut(s) 198
AvaII GGWCC 2 cut(s) 70, 190
BbsI GAAGAC 2 cut(s) 34, 172
BbvI GCAGC 4 cut(s) 45, 330, 333, 367
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 17, 155
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 5 cut(s) 34, 319, 322, 347, 381
BlsI GCNGC 5 cut(s) 35, 320, 323, 348, 382
Bme1390I CCNGG 2 cut(s) 80, 198
Bme18I GGWCC 2 cut(s) 70, 190
BmgT120I GGNCC 3 cut(s) 70, 89, 190
BmiI GGNNCC 2 cut(s) 71, 72
BmrFI CCNGG 2 cut(s) 80, 198
BpiI GAAGAC 2 cut(s) 34, 172
BpmI CTGGAG 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 79
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 80
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 166
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 79
BseLI CCNNNNNNNGG 2 cut(s) 79, 80
BseMI GCAATG 1 cut(s) 28
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 6 cut(s) 94, 97, 280, 296, 328, 349
BseXI GCAGC 4 cut(s) 45, 330, 333, 367
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 27, 90
BsiSI CCGG 5 cut(s) 117, 167, 198, 350, 384
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 79, 80
BsmAI GTCTC 2 cut(s) 17, 155
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 27, 90
Bsp13I TCCGGA 1 cut(s) 166
Bsp143I GATC 3 cut(s) 252, 367, 401
BspACI CCGC 5 cut(s) 114, 129, 188, 258, 321
BspANI GGCC 2 cut(s) 27, 90
BspEI TCCGGA 1 cut(s) 166
BspFNI CGCG 1 cut(s) 137
BspHI TCATGA 2 cut(s) 96, 330
BspLI GGNNCC 2 cut(s) 71, 72
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 2 cut(s) 375, 409
BsrDI GCAATG 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 3 cut(s) 252, 367, 401
Bst2UI CCWGG 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 8
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 3 cut(s) 255, 370, 404
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 3 cut(s) 252, 367, 401
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 196
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 4 cut(s) 45, 330, 333, 367
BstV2I GAAGAC 2 cut(s) 34, 172
BstXI CCANNNNNNTGG 1 cut(s) 86
BsuRI GGCC 2 cut(s) 27, 90
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 118
CciI TCATGA 2 cut(s) 96, 330
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 70, 89, 190
CseI GACGC 1 cut(s) 39
CviAII CATG 3 cut(s) 97, 176, 331
CviJI RGCY 7 cut(s) 5, 27, 90, 173, 229, 346, 380
CviKI_1 RGCY 7 cut(s) 5, 27, 90, 173, 229, 346, 380
DdeI CTNAG 1 cut(s) 8
DpnI GATC 3 cut(s) 254, 369, 403
DpnII GATC 3 cut(s) 252, 367, 401
DraIII CACNNNGTG 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 25
EciI GGCGGA 2 cut(s) 118, 203
Eco47I GGWCC 2 cut(s) 70, 190
Eco57I CTGAAG 2 cut(s) 224, 233
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 100, 179, 334
FaiI YATR 3 cut(s) 98, 177, 332
FaqI GGGAC 1 cut(s) 83
FatI CATG 3 cut(s) 96, 175, 330
Fnu4HI GCNGC 5 cut(s) 34, 319, 322, 347, 381
Fsp4HI GCNGC 5 cut(s) 34, 319, 322, 347, 381
GlaI GCGC 1 cut(s) 138
GluI GCNGC 5 cut(s) 34, 319, 322, 347, 381
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 2 cut(s) 27, 90
HapII CCGG 5 cut(s) 117, 167, 198, 350, 384
HgaI GACGC 1 cut(s) 39
HhaI GCGC 1 cut(s) 139
Hin1II CATG 3 cut(s) 100, 179, 334
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HinfI GANTC 2 cut(s) 147, 163
HpaII CCGG 5 cut(s) 117, 167, 198, 350, 384
Hpy188I TCNGA 1 cut(s) 252
Hpy188III TCNNGA 3 cut(s) 97, 167, 331
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 7 cut(s) 85, 142, 151, 202, 214, 230, 257
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 359, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 3 cut(s) 100, 179, 334
HspAI GCGC 1 cut(s) 137
KflI GGGWCCC 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 166
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 252, 367, 401
LmnI GCTCC 3 cut(s) 267, 274, 315
Lsp1109I GCAGC 4 cut(s) 45, 330, 333, 367
MaeII ACGT 1 cut(s) 39
MalI GATC 3 cut(s) 254, 369, 403
MboI GATC 3 cut(s) 252, 367, 401
MboII GAAGA 5 cut(s) 34, 172, 217, 224, 298
MlyI GAGTC 1 cut(s) 157
MreI CGCCGGCG 1 cut(s) 116
MroI TCCGGA 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 1 cut(s) 409
MspA1I CMGCKG 2 cut(s) 258, 321
MspI CCGG 5 cut(s) 117, 167, 198, 350, 384
MspR9I CCNGG 2 cut(s) 80, 198
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 33
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 198
NdeII GATC 3 cut(s) 252, 367, 401
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 3 cut(s) 100, 179, 334
NlaIV GGNNCC 2 cut(s) 71, 72
PagI TCATGA 2 cut(s) 96, 330
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 5 cut(s) 35, 320, 323, 348, 382
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 71, 72
PspPI GGNCC 3 cut(s) 70, 89, 190
PspPPI RGGWCCY 1 cut(s) 70
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 5 cut(s) 34, 319, 322, 347, 381
Sau3AI GATC 3 cut(s) 252, 367, 401
Sau96I GGNCC 3 cut(s) 70, 89, 190
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 2 cut(s) 80, 198
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 2 cut(s) 70, 190
SsiI CCGC 5 cut(s) 114, 129, 188, 258, 321
StyD4I CCNGG 2 cut(s) 78, 196
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 45, 60, 150
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TseI GCWGC 4 cut(s) 33, 318, 346, 380
TspDTI ATGAA 2 cut(s) 113, 319
VpaK11BI GGWCC 2 cut(s) 70, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.