MD07G1210700.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003719
Physical Location & Seq
Forward (+)
28969132 .. 28969626
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1210700.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCTCTCAGTCTCTTTGGCAACGGCCGACGAAGCAGCGACTTCGACCCCTTCTCTCTCGACATCTGGGACCCGTTTGAGGGCTTGGGCACTCTGGCCAACTTCCCCTCCTCCGCCCGTGAAACCACCGCCATAGCCAACACCCGCATCGACTGGAAGGAGACCCCGGAGGCCCACATCTTCAAGGCGGACCTCCCGGGGATCAAAAAGGAGGAGGTGAAAGTGGAGGTGGAGGACGGGAGGGTCCTGCAGATCAGCGGCGAGAGGAGCAGGGAGCAGGAGGATCAGAACGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGCAAGTTCGTAAGGAGGTTCAGGCTGCCGGAGAACGCGAAGATTGATCAGGTGAAGGCGGCGATGGAGAATGGGGTGCTGACTGTGACTGTGCCGAAAGAGGAGGAGAAGAAGCCCGAGGTCAAGGCCATTGAAATCTCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

156

Amino Acids

17.55

Weight (kDa)

5.39

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 154 2e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccII CGCG 1 cut(s) 359
AciI CCGC 7 cut(s) 114, 129, 145, 188, 258, 321, 380
AclWI GGATC 2 cut(s) 209, 291
AcoI YGGCCR 2 cut(s) 25, 96
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 79, 80
AgsI TTSAA 2 cut(s) 184, 455
Alw26I GTCTC 2 cut(s) 17, 155
AlwI GGATC 2 cut(s) 209, 291
Ama87I CYCGRG 2 cut(s) 196, 437
AoxI GGCC 4 cut(s) 25, 96, 171, 447
ApeKI GCWGC 3 cut(s) 36, 318, 346
AspS9I GGNCC 4 cut(s) 70, 172, 190, 244
AsuC2I CCSGG 3 cut(s) 167, 197, 198
AsuHPI GGTGA 2 cut(s) 229, 385
AvaI CYCGRG 2 cut(s) 196, 437
AvaII GGWCC 3 cut(s) 70, 190, 244
BaeGI GKGCMC 1 cut(s) 92
BalI TGGCCA 1 cut(s) 98
BbvI GCAGC 3 cut(s) 48, 330, 333
BccI CCATC 1 cut(s) 379
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 3 cut(s) 167, 197, 198
BcoDI GTCTC 2 cut(s) 17, 155
BfmI CTRYAG 1 cut(s) 248
BisI GCNGC 6 cut(s) 37, 259, 319, 322, 347, 381
BlsI GCNGC 6 cut(s) 38, 260, 320, 323, 348, 382
Bme1390I CCNGG 3 cut(s) 167, 197, 198
Bme18I GGWCC 3 cut(s) 70, 190, 244
BmeT110I CYCGRG 2 cut(s) 196, 437
BmgT120I GGNCC 4 cut(s) 70, 172, 190, 244
BmiI GGNNCC 3 cut(s) 71, 72, 245
BmrFI CCNGG 3 cut(s) 167, 197, 198
BmsI GCATC 1 cut(s) 156
BpuMI CCSGG 3 cut(s) 167, 197, 198
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 4 cut(s) 165, 196, 197, 438
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 80
Bse1I ACTGG 1 cut(s) 158
BseDI CCNNGG 4 cut(s) 165, 196, 197, 438
BseLI CCNNNNNNNGG 2 cut(s) 79, 80
BseMII CTCAG 1 cut(s) 22
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 6 cut(s) 100, 227, 280, 328, 437, 440
BseSI GKGCMC 1 cut(s) 92
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 48, 330, 333
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 4 cut(s) 27, 98, 173, 449
BsiEI CGRYCG 1 cut(s) 28
BsiHKCI CYCGRG 2 cut(s) 196, 437
BsiSI CCGG 4 cut(s) 167, 197, 350, 462
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 79, 80
BsmAI GTCTC 2 cut(s) 17, 155
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 4 cut(s) 27, 98, 173, 449
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 2 cut(s) 196, 437
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 4 cut(s) 201, 252, 283, 367
BspACI CCGC 7 cut(s) 114, 129, 145, 188, 258, 321, 380
BspANI GGCC 4 cut(s) 27, 98, 173, 449
BspCNI CTCAG 1 cut(s) 21
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 3 cut(s) 71, 72, 245
BspMAI CTGCAG 1 cut(s) 252
BspPI GGATC 2 cut(s) 209, 291
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 4 cut(s) 165, 196, 197, 438
BssMI GATC 4 cut(s) 201, 252, 283, 367
Bst4CI ACNGT 2 cut(s) 406, 412
BstDEI CTNAG 1 cut(s) 8
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 4 cut(s) 204, 255, 286, 370
BstMAI GTCTC 2 cut(s) 17, 155
BstMBI GATC 4 cut(s) 201, 252, 283, 367
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 33, 267
BstSCI CCNGG 3 cut(s) 165, 195, 196
BstSFI CTRYAG 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 92
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 3 cut(s) 48, 330, 333
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 4 cut(s) 27, 98, 173, 449
BtgZI GCGATG 1 cut(s) 398
Cfr13I GGNCC 4 cut(s) 70, 172, 190, 244
Cfr9I CCCGGG 1 cut(s) 196
DdeI CTNAG 1 cut(s) 8
DpnI GATC 4 cut(s) 203, 254, 285, 369
DpnII GATC 4 cut(s) 201, 252, 283, 367
DraIII CACNNNGTG 1 cut(s) 307
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 25, 96
EagI CGGCCG 1 cut(s) 25
EciI GGCGGA 2 cut(s) 103, 203
EclXI CGGCCG 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 3 cut(s) 70, 190, 244
Eco52I CGGCCG 1 cut(s) 25
Eco88I CYCGRG 2 cut(s) 196, 437
EcoO109I RGGNCCY 2 cut(s) 70, 244
FaiI YATR 1 cut(s) 134
FaqI GGGAC 1 cut(s) 83
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 37, 259, 319, 322, 347, 381
Fsp4HI GCNGC 6 cut(s) 37, 259, 319, 322, 347, 381
GluI GCNGC 6 cut(s) 37, 259, 319, 322, 347, 381
HaeIII GGCC 4 cut(s) 27, 98, 173, 449
HapII CCGG 4 cut(s) 167, 197, 350, 462
HpaII CCGG 4 cut(s) 167, 197, 350, 462
HphI GGTGA 2 cut(s) 229, 385
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 1 cut(s) 59
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 3 cut(s) 61, 151, 370
HpyCH4III ACNGT 2 cut(s) 406, 412
HpyCH4V TGCA 1 cut(s) 250
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 267
HpyF3I CTNAG 1 cut(s) 8
KflI GGGWCCC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 201, 252, 283, 367
LmnI GCTCC 3 cut(s) 267, 274, 315
Lsp1109I GCAGC 3 cut(s) 48, 330, 333
LweI GCATC 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 203, 254, 285, 369
MboI GATC 4 cut(s) 201, 252, 283, 367
MboII GAAGA 3 cut(s) 172, 373, 442
MhlI GDGCHC 1 cut(s) 92
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
Mox20I TGGCCA 1 cut(s) 98
MscI TGGCCA 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 2 cut(s) 258, 321
MspI CCGG 4 cut(s) 167, 197, 350, 462
MspR9I CCNGG 3 cut(s) 167, 197, 198
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 33, 267
NciI CCSGG 3 cut(s) 167, 197, 198
NdeII GATC 4 cut(s) 201, 252, 283, 367
NlaIV GGNNCC 3 cut(s) 71, 72, 245
NmuCI GTSAC 1 cut(s) 406
PkrI GCNGC 6 cut(s) 38, 260, 320, 323, 348, 382
PpuMI RGGWCCY 2 cut(s) 70, 244
Psp5II RGGWCCY 2 cut(s) 70, 244
PspN4I GGNNCC 3 cut(s) 71, 72, 245
PspPI GGNCC 4 cut(s) 70, 172, 190, 244
PspPPI RGGWCCY 2 cut(s) 70, 244
PstI CTGCAG 1 cut(s) 252
SatI GCNGC 6 cut(s) 37, 259, 319, 322, 347, 381
Sau3AI GATC 4 cut(s) 201, 252, 283, 367
Sau96I GGNCC 4 cut(s) 70, 172, 190, 244
ScrFI CCNGG 3 cut(s) 167, 197, 198
SduI GDGCHC 1 cut(s) 92
SetI ASST 6 cut(s) 195, 219, 231, 341, 375, 444
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 248
SinI GGWCC 3 cut(s) 70, 190, 244
SmaI CCCGGG 1 cut(s) 198
SsiI CCGC 7 cut(s) 114, 129, 145, 188, 258, 321, 380
StyD4I CCNGG 3 cut(s) 165, 195, 196
TaaI ACNGT 2 cut(s) 406, 412
TaqI TCGA 3 cut(s) 45, 60, 150
TauI GCSGC 3 cut(s) 261, 324, 383
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 3 cut(s) 36, 318, 346
Tsp45I GTSAC 1 cut(s) 406
TspMI CCCGGG 1 cut(s) 196
VpaK11BI GGWCC 3 cut(s) 70, 190, 244
XmaI CCCGGG 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.