AT1G55170

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003143
Physical Location & Seq
Forward (+)
20580274 .. 20581975
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G55170.1

Sequence Viewer

Length: 852 bp
ATGTCTGGAAGAAACCGGATTCACAGAGATATCCGAGACAGCTATCATGATCATCGTGATTTACCTCCGGAGAGACCTTTTCTCCGAGGCCCTCCATTGTTACAACCTCCACCACCATCTTTGTTAGAAGATCTTCAGATACAGGAGGGTGAAATCAGAAGGCAGGATGCTGAAATCAGAAGGCTTTTGAGTGATAATCATGGACTAGCTGACGACCGAATGGTTCTGGAGAGAGAACTCGTTGCTGCTAAAGAAGAGCTTCACCGAATGAATCTGATGATTTCTGATCTTCGTGCTGAGCAAGATCTGCAATTGAGGGAGTTTAGTGAGAAAAGGCATAAACTTGAGGGGGATGTTCGCGCTATGGAGTCGTATAAGAAGGAGGCTTCACAGTTGCGTGGTGAAGTTCAGAAGCTGGATGAGATTAAACGTGAATTAAGTGGAAATGTTCAGTTGCTTAGGAAAGATTTGGCTAAATTGCAATCAGATAACAAGCAGATTCCTGGTATGAGAGCTGAGGTTAAAGACCTGCAGAAGGAGCTTATGCATGCTAGAGATGCAATTGAATATGAGAAGAAGGAAAAGTTTGAGCTTATGGAGCAGCGGCAGACAATGGAAAAGAACATGGTTTCTATGGCGCGTGAAGTTGAAAAGCTACGCGCAGAACTTGCTACTGTTGATAGTAGGCCGTGGGGTTTTGGTGGATCGTATGGGATGAATTACAACAATATGGACGGCACTTTCCGTGGCTCGTATGGCGAAAACGATACTTATTTGGGATCTTCGGAAAGGAGTCAATATTACAGCCATGGGTCTGGCTCGCAGAAGAAACCTCGTTTGGATCGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

33.1

Weight (kDa)

6.54

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 537
AccII CGCG 3 cut(s) 360, 640, 660
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 1 cut(s) 604
AclWI GGATC 3 cut(s) 712, 787, 849
AcuI CTGAAG 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 535
AgsI TTSAA 2 cut(s) 566, 650
AjnI CCWGG 1 cut(s) 502
AjuI GAANNNNNNNTTGG 1 cut(s) 821
AluBI AGCT 8 cut(s) 42, 209, 259, 415, 515, 541, 592, 655
AluI AGCT 8 cut(s) 42, 209, 259, 415, 515, 541, 592, 655
Alw26I GTCTC 2 cut(s) 30, 67
AlwI GGATC 3 cut(s) 712, 787, 849
AlwNI CAGNNNCTG 1 cut(s) 415
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 2 cut(s) 88, 686
ApeKI GCWGC 2 cut(s) 245, 601
Asp700I GAANNNNTTC 2 cut(s) 132, 258
AspLEI GCGC 3 cut(s) 362, 640, 662
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 3 cut(s) 161, 254, 413
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 232, 613
BccI CCATC 1 cut(s) 124
BceAI ACGGC 2 cut(s) 673, 751
BciT130I CCWGG 1 cut(s) 504
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 30, 67
BfaI CTAG 2 cut(s) 206, 552
BfmI CTRYAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 537
BglII AGATCT 2 cut(s) 130, 304
BisI GCNGC 3 cut(s) 246, 602, 605
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 3 cut(s) 247, 603, 606
Bme1390I CCNGG 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 504
BmsI GCATC 2 cut(s) 157, 547
BpmI CTGGAG 1 cut(s) 248
Bpu10I CCTNAGC 2 cut(s) 458, 516
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 4 cut(s) 85, 689, 745, 808
BsaWI WCCGGW 2 cut(s) 15, 67
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 4 cut(s) 85, 689, 745, 808
BseGI GGATG 4 cut(s) 172, 358, 424, 720
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 2 cut(s) 288, 507
BseXI GCAGC 2 cut(s) 232, 613
Bsh1236I CGCG 3 cut(s) 360, 640, 660
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 90, 688
BsiEI CGRYCG 1 cut(s) 217
BsiSI CCGG 2 cut(s) 16, 68
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 2 cut(s) 30, 67
BsnI GGCC 2 cut(s) 90, 688
Bso31I GGTCTC 1 cut(s) 67
Bsp13I TCCGGA 1 cut(s) 67
Bsp143I GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 1 cut(s) 808
BspACI CCGC 1 cut(s) 604
BspANI GGCC 2 cut(s) 90, 688
BspCNI CTCAG 2 cut(s) 289, 508
BspEI TCCGGA 1 cut(s) 67
BspFNI CGCG 3 cut(s) 360, 640, 660
BspHI TCATGA 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 534
BspMI ACCTGC 1 cut(s) 537
BspPI GGATC 3 cut(s) 712, 787, 849
BspQI GCTCTTC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 67
BssECI CCNNGG 4 cut(s) 85, 689, 745, 808
BssMI GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
BssT1I CCWWGG 1 cut(s) 808
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 393, 676
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 2 cut(s) 307, 668
BstC8I GCNNGC 2 cut(s) 549, 821
BstDEI CTNAG 3 cut(s) 297, 458, 516
BstDSI CCRYGG 3 cut(s) 689, 745, 808
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 4 cut(s) 172, 358, 424, 720
BstFNI CGCG 3 cut(s) 360, 640, 660
BstHHI GCGC 3 cut(s) 362, 640, 662
BstKTI GATC 7 cut(s) 52, 133, 289, 307, 707, 782, 844
BstMAI GTCTC 2 cut(s) 30, 67
BstMBI GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 6 cut(s) 307, 538, 557, 598, 668, 756
BstNI CCWGG 1 cut(s) 504
BstNSI RCATGY 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 502
BstSFI CTRYAG 1 cut(s) 530
BstUI CGCG 3 cut(s) 360, 640, 660
BstV1I GCAGC 2 cut(s) 232, 613
BstX2I RGATCY 3 cut(s) 130, 304, 779
BstXI CCANNNNNNTGG 1 cut(s) 815
BstYI RGATCY 3 cut(s) 130, 304, 779
BsuRI GGCC 2 cut(s) 90, 688
BtgI CCRYGG 3 cut(s) 689, 745, 808
BtsCI GGATG 4 cut(s) 172, 358, 424, 720
BveI ACCTGC 1 cut(s) 537
Cac8I GCNNGC 2 cut(s) 549, 821
CaiI CAGNNNCTG 1 cut(s) 415
CciI TCATGA 1 cut(s) 46
CfoI GCGC 3 cut(s) 362, 640, 662
Cfr13I GGNCC 1 cut(s) 89
CspCI CAANNNNNGTGG 2 cut(s) 102, 137
CviAII CATG 5 cut(s) 47, 200, 548, 625, 809
DdeI CTNAG 3 cut(s) 297, 458, 516
DpnI GATC 7 cut(s) 51, 132, 288, 306, 706, 781, 843
DpnII GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 808
Eco31I GGTCTC 1 cut(s) 67
Eco32I GATATC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoO109I RGGNCCY 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 502
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 808
EcoT22I ATGCAT 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 5 cut(s) 50, 203, 551, 628, 812
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FatI CATG 5 cut(s) 46, 199, 547, 624, 808
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 246, 602, 605
FokI GGATG 4 cut(s) 179, 365, 431, 727
Fsp4HI GCNGC 3 cut(s) 246, 602, 605
FspBI CTAG 2 cut(s) 206, 552
GlaI GCGC 3 cut(s) 361, 639, 661
GluI GCNGC 3 cut(s) 246, 602, 605
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 2 cut(s) 90, 688
HapII CCGG 2 cut(s) 16, 68
HhaI GCGC 3 cut(s) 362, 640, 662
Hin1II CATG 5 cut(s) 50, 203, 551, 628, 812
Hin6I GCGC 3 cut(s) 360, 638, 660
HinP1I GCGC 3 cut(s) 360, 638, 660
HinfI GANTC 5 cut(s) 19, 271, 368, 499, 793
HpaII CCGG 2 cut(s) 16, 68
HphI GGTGA 3 cut(s) 161, 254, 413
Hpy188III TCNNGA 5 cut(s) 6, 47, 56, 68, 227
HpyAV CCTTC 5 cut(s) 153, 174, 373, 529, 571
HpyCH4III ACNGT 2 cut(s) 393, 676
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 5 cut(s) 310, 481, 532, 547, 560
HpyF10VI GCNNNNNNNGC 6 cut(s) 307, 538, 557, 598, 668, 756
HpyF3I CTNAG 3 cut(s) 297, 458, 516
HpySE526I ACGT 1 cut(s) 430
Hsp92II CATG 5 cut(s) 50, 203, 551, 628, 812
HspAI GCGC 3 cut(s) 360, 638, 660
Kpn2I TCCGGA 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 2 cut(s) 538, 598
Lsp1109I GCAGC 2 cut(s) 232, 613
LweI GCATC 2 cut(s) 157, 547
MaeI CTAG 2 cut(s) 206, 552
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 1 cut(s) 99
MalI GATC 7 cut(s) 51, 132, 288, 306, 706, 781, 843
MboI GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
MboII GAAGA 8 cut(s) 21, 125, 140, 266, 281, 586, 774, 838
MfeI CAATTG 2 cut(s) 311, 561
MflI RGATCY 3 cut(s) 130, 304, 779
MluCI AATT 5 cut(s) 311, 434, 476, 561, 718
MlyI GAGTC 2 cut(s) 377, 802
Mph1103I ATGCAT 1 cut(s) 549
MroI TCCGGA 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 132, 258
MseI TTAA 3 cut(s) 426, 437, 522
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 2 cut(s) 16, 68
MspR9I CCNGG 1 cut(s) 504
MunI CAATTG 2 cut(s) 311, 561
MvaI CCWGG 1 cut(s) 504
MvnI CGCG 3 cut(s) 360, 640, 660
MwoI GCNNNNNNNGC 6 cut(s) 307, 538, 557, 598, 668, 756
NcoI CCATGG 1 cut(s) 808
NdeII GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
NlaIII CATG 5 cut(s) 50, 203, 551, 628, 812
NsiI ATGCAT 1 cut(s) 549
NspI RCATGY 1 cut(s) 551
PaeI GCATGC 1 cut(s) 551
PagI TCATGA 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 841
PdmI GAANNNNTTC 2 cut(s) 132, 258
PfeI GAWTC 3 cut(s) 19, 271, 499
PkrI GCNGC 3 cut(s) 247, 603, 606
PleI GAGTC 2 cut(s) 376, 801
PpsI GAGTC 2 cut(s) 376, 801
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 534
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 3 cut(s) 130, 304, 779
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 426, 437, 522
SatI GCNGC 3 cut(s) 246, 602, 605
Sau3AI GATC 7 cut(s) 49, 130, 286, 304, 704, 779, 841
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 2 cut(s) 377, 802
ScrFI CCNGG 1 cut(s) 504
SfaNI GCATC 2 cut(s) 157, 547
SfcI CTRYAG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SphI GCATGC 1 cut(s) 551
Sse9I AATT 5 cut(s) 311, 434, 476, 561, 718
SsiI CCGC 1 cut(s) 604
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 206, 552
StyD4I CCNGG 1 cut(s) 502
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 2 cut(s) 393, 676
TaiI ACGT 1 cut(s) 433
TaqII GACCGA 1 cut(s) 231
TasI AATT 5 cut(s) 311, 434, 476, 561, 718
TauI GCSGC 1 cut(s) 607
TfiI GAWTC 3 cut(s) 19, 271, 499
Tru1I TTAA 3 cut(s) 426, 437, 522
Tru9I TTAA 3 cut(s) 426, 437, 522
TseI GCWGC 2 cut(s) 245, 601
TspDTI ATGAA 2 cut(s) 284, 731
TspGWI ACGGA 1 cut(s) 734
XagI CCTNNNNNAGG 1 cut(s) 533
XceI RCATGY 1 cut(s) 551
XmnI GAANNNNTTC 2 cut(s) 132, 258
XspI CTAG 2 cut(s) 206, 552
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.