Rh5DG449800

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003143
Physical Location & Seq
Forward (+)
71745544 .. 71746764
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG449800.1

Sequence Viewer

Length: 378 bp
ATGGTTTTTAGCTATCAACAGATTCCTTTGCTAAAGTCTGAGATTGATGGTCTGCACCAGGAGCTTATGCATACTAGAAGTGCAATTGATTATGAAAAGAAGGCAAATATTGAGCTTATGGAACAGAGACAGGCAATGGAGAATAACCTAGTTTCTATGGTTTGTGAAGTTGAGAAACTTCGTGCAGAACTTGCTAGAGTTGATGGTAGACCGTGGGGTGCAGTTTTTGGTGGCAAAAAAGCTCGTACTAGTGCACCTGCTGTCGCTCCTACTGTGGCTCACGCTCTCAAGAAAGAGGATGCTCCCGAAGTCCGTCAACCTCAGAGACCTAAACCCATGCAATCTGCAGGGAGTGATCTCTTGACCAGGGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.97

Weight (kDa)

7.89

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 208
AfaI GTAC 1 cut(s) 247
AhlI ACTAGT 1 cut(s) 248
AjnI CCWGG 2 cut(s) 57, 365
AluBI AGCT 4 cut(s) 12, 64, 115, 242
AluI AGCT 4 cut(s) 12, 64, 115, 242
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 121, 319
Alw44I GTGCAC 1 cut(s) 252
ApaLI GTGCAC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 256
BccI CCATC 2 cut(s) 41, 197
BciT130I CCWGG 2 cut(s) 59, 367
BcoDI GTCTC 2 cut(s) 121, 319
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 4 cut(s) 75, 149, 195, 249
BfmI CTRYAG 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 59, 367
BmrFI CCNGG 2 cut(s) 59, 367
BmsI GCATC 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 212, 366
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 2 cut(s) 59, 367
BseDI CCNNGG 2 cut(s) 212, 366
BseGI GGATG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 30, 335
BseSI GKGCMC 1 cut(s) 256
BsgI GTGCAG 3 cut(s) 38, 204, 240
BsiHKAI GWGCWC 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 121, 319
Bso31I GGTCTC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 1 cut(s) 355
BspCNI CTCAG 2 cut(s) 31, 334
BspMAI CTGCAG 1 cut(s) 349
BspMI ACCTGC 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 212, 366
BssMI GATC 1 cut(s) 355
Bst2UI CCWGG 2 cut(s) 59, 367
Bst4CI ACNGT 2 cut(s) 213, 274
BstAPI GCANNNNNTGC 1 cut(s) 191
BstDEI CTNAG 2 cut(s) 39, 321
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 2 cut(s) 121, 319
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 2 cut(s) 61, 191
BstNI CCWGG 2 cut(s) 59, 367
BstSCI CCNGG 2 cut(s) 57, 365
BstSFI CTRYAG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 246
CviAII CATG 1 cut(s) 337
CviJI RGCY 5 cut(s) 12, 64, 115, 242, 278
CviKI_1 RGCY 5 cut(s) 12, 64, 115, 242, 278
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 2 cut(s) 39, 321
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 57, 365
EcoT22I ATGCAT 1 cut(s) 72
FaeI CATG 1 cut(s) 340
FaiI YATR 7 cut(s) 68, 72, 93, 119, 158, 338, 376
FatI CATG 1 cut(s) 336
FblI GTMKAC 1 cut(s) 208
FokI GGATG 1 cut(s) 311
FspBI CTAG 4 cut(s) 75, 149, 195, 249
Hin1II CATG 1 cut(s) 340
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HinfI GANTC 1 cut(s) 22
Hpy166II GTNNAC 3 cut(s) 209, 254, 317
Hpy188I TCNGA 2 cut(s) 40, 324
Hpy188III TCNNGA 3 cut(s) 289, 305, 361
Hpy8I GTNNAC 3 cut(s) 209, 254, 317
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 213, 274
HpyCH4V TGCA 8 cut(s) 55, 70, 83, 185, 221, 254, 340, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 191
HpyF3I CTNAG 2 cut(s) 39, 321
Hsp92II CATG 1 cut(s) 340
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 3 cut(s) 61, 271, 307
LpnPI CCDG 6 cut(s) 44, 71, 116, 270, 333, 352
LweI GCATC 1 cut(s) 289
MaeI CTAG 4 cut(s) 75, 149, 195, 249
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MfeI CAATTG 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 1 cut(s) 84
MnlI CCTC 2 cut(s) 289, 330
Mph1103I ATGCAT 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 59, 367
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 2 cut(s) 59, 367
MwoI GCNNNNNNNGC 2 cut(s) 61, 191
NdeII GATC 1 cut(s) 355
NlaIII CATG 1 cut(s) 340
NsiI ATGCAT 1 cut(s) 72
PaqCI CACCTGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 22
Psp6I CCWGG 2 cut(s) 57, 365
PspGI CCWGG 2 cut(s) 57, 365
PstI CTGCAG 1 cut(s) 349
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
Sau3AI GATC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 59, 367
SduI GDGCHC 1 cut(s) 256
SetI ASST 8 cut(s) 14, 66, 117, 150, 244, 259, 322, 331
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 248
Sse9I AATT 1 cut(s) 84
SspI AATATT 1 cut(s) 109
SspMI CTAG 4 cut(s) 75, 149, 195, 249
StyD4I CCNGG 2 cut(s) 57, 365
TaaI ACNGT 2 cut(s) 213, 274
TasI AATT 1 cut(s) 84
TfiI GAWTC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 108
TspGWI ACGGA 1 cut(s) 302
VneI GTGCAC 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 208
XspI CTAG 4 cut(s) 75, 149, 195, 249
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.