Rroxscaffold_2G00103290

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003143
Physical Location & Seq
Forward (+)
25814575 .. 25816893
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103290.1

Sequence Viewer

Length: 774 bp
ATGGTAGAGCCCCTCCTGTGCCTCGGCAGCTGCCCCCATCCTGCAATGTTGGAGGAAGAACTTGAAATGCAGCATGCTGAGATTCAGAGGCTCTTAGCTGATAACCGGAGGCTGGTTGAGGACCGAATGGCATTGCAGCATGATCTCGGTGCTGCCAAGGAGGAAATTCATCGCATGAATGTTGCCTTTTCTGATATTCGTGCCGAGGAAGAACTGCAGTCGAGGGAGCTCTTTGAGAAAGCAAGAAAATTGGAAGCTGATCTTCGGGGAGCTGAGCAGTTTAAAAATGAGGCTAAACAACTACGAGGTGAAGTTCAGAAGCTGAATGGTGTTAGGAAGGAACTTTCCACACAAGTGCAGACTCTCACACAGGACCTTGTAAAGTTGCAAGCTGATAATCAACAGATTCCTTTGCTAAAGTCTGAGATTGATGGTCTGCACACCAGGAGCTTATGCATGCTAGAAGTGCAATTGATTATGAAAAAGAAGGCAAATATTGAGCTTTTGGAACAGAGACAGGCAATGGAGAGTAACCTAGTTTCTATGGCTCGTGAAGTTGAGAAACTTCGTGCAGAGCTTGCTAGAGTTGATGGTAGACCGTGGGGTGCAGGTGGACAATATGGGATGAGTTTCAGCAGCCCAGATGGAAGCTTTCCTGCTCCGTATGGAGATGGATATGGGATGCATATGGGTATGCCTGATAAAGGACCGATGTATGGGCCAGGCCCAACTTTGTGGGGAGGTTCGGAAAAGCCGCGCATGCCTCGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

29.02

Weight (kDa)

5.79

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 599
AccI GTMKAC 1 cut(s) 595
AccII CGCG 1 cut(s) 757
AciI CCGC 1 cut(s) 755
AcsI RAATTY 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 112, 704, 716
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 2 cut(s) 443, 721
AleI CACNNNNGTG 1 cut(s) 353
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 508
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 2 cut(s) 719, 724
ApeKI GCWGC 6 cut(s) 27, 30, 70, 136, 152, 636
ApoI RAATTY 1 cut(s) 165
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 5 cut(s) 121, 373, 707, 719, 725
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 3 cut(s) 121, 373, 707
BanII GRGCYC 2 cut(s) 12, 231
BauI CACGAG 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 6 cut(s) 17, 39, 82, 139, 148, 648
BccI CCATC 5 cut(s) 45, 425, 584, 638, 665
BciT130I CCWGG 2 cut(s) 445, 723
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 3 cut(s) 461, 536, 582
BfmI CTRYAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 599
BisI GCNGC 7 cut(s) 28, 31, 71, 137, 153, 637, 755
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 7 cut(s) 29, 32, 72, 138, 154, 638, 756
Bme1390I CCNGG 2 cut(s) 445, 723
Bme18I GGWCC 3 cut(s) 121, 373, 707
BmgT120I GGNCC 5 cut(s) 121, 373, 707, 719, 725
BmrFI CCNGG 2 cut(s) 445, 723
BmsI GCATC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 273
BsaJI CCNNGG 4 cut(s) 22, 156, 204, 599
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 704, 716
Bse3DI GCAATG 3 cut(s) 51, 131, 528
BseBI CCWGG 2 cut(s) 445, 723
BseDI CCNNGG 4 cut(s) 22, 156, 204, 599
BseGI GGATG 3 cut(s) 37, 630, 687
BseLI CCNNNNNNNGG 3 cut(s) 112, 704, 716
BseMI GCAATG 3 cut(s) 51, 131, 528
BseMII CTCAG 3 cut(s) 69, 264, 414
BseXI GCAGC 6 cut(s) 17, 39, 82, 139, 148, 648
BsgI GTGCAG 4 cut(s) 377, 422, 591, 627
Bsh1236I CGCG 1 cut(s) 757
BshFI GGCC 2 cut(s) 721, 726
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 106
BslI CCNNNNNNNGG 3 cut(s) 112, 704, 716
BsmAI GTCTC 1 cut(s) 508
BsnI GGCC 2 cut(s) 721, 726
Bsp1286I GDGCHC 2 cut(s) 12, 231
Bsp143I GATC 2 cut(s) 142, 259
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 1 cut(s) 755
BspANI GGCC 2 cut(s) 721, 726
BspCNI CTCAG 3 cut(s) 70, 265, 415
BspFNI CGCG 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 599
BsrDI GCAATG 3 cut(s) 51, 131, 528
BssECI CCNNGG 4 cut(s) 22, 156, 204, 599
BssMI GATC 2 cut(s) 142, 259
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 445, 723
Bst4CI ACNGT 1 cut(s) 600
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 5 cut(s) 75, 390, 458, 579, 761
BstDEI CTNAG 4 cut(s) 78, 94, 273, 423
BstDSI CCRYGG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 702
BstF5I GGATG 3 cut(s) 37, 630, 687
BstFNI CGCG 1 cut(s) 757
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 2 cut(s) 145, 262
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 2 cut(s) 142, 259
BstMWI GCNNNNNNNGC 4 cut(s) 27, 466, 578, 760
BstNI CCWGG 2 cut(s) 445, 723
BstNSI RCATGY 3 cut(s) 77, 460, 763
BstSCI CCNGG 2 cut(s) 443, 721
BstSFI CTRYAG 1 cut(s) 215
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 6 cut(s) 17, 39, 82, 139, 148, 648
BstXI CCANNNNNNTGG 1 cut(s) 735
BsuRI GGCC 2 cut(s) 721, 726
BtgI CCRYGG 1 cut(s) 599
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 3 cut(s) 37, 630, 687
BveI ACCTGC 1 cut(s) 599
Cac8I GCNNGC 5 cut(s) 75, 390, 458, 579, 761
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 1 cut(s) 759
Cfr13I GGNCC 5 cut(s) 121, 373, 707, 719, 725
CviAII CATG 5 cut(s) 74, 140, 175, 457, 760
DdeI CTNAG 4 cut(s) 78, 94, 273, 423
DpnI GATC 2 cut(s) 144, 261
DpnII GATC 2 cut(s) 142, 259
DraI TTTAAA 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 2 cut(s) 12, 231
Eco47I GGWCC 3 cut(s) 121, 373, 707
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 443, 721
EcoT14I CCWWGG 1 cut(s) 156
EcoT22I ATGCAT 2 cut(s) 458, 687
EcoT38I GRGCYC 2 cut(s) 12, 231
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 5 cut(s) 77, 143, 178, 460, 763
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FatI CATG 5 cut(s) 73, 139, 174, 456, 759
FauNDI CATATG 1 cut(s) 687
FblI GTMKAC 1 cut(s) 595
Fnu4HI GCNGC 7 cut(s) 28, 31, 71, 137, 153, 637, 755
FokI GGATG 3 cut(s) 24, 637, 694
FriOI GRGCYC 2 cut(s) 12, 231
Fsp4HI GCNGC 7 cut(s) 28, 31, 71, 137, 153, 637, 755
FspBI CTAG 3 cut(s) 461, 536, 582
GlaI GCGC 1 cut(s) 758
GluI GCNGC 7 cut(s) 28, 31, 71, 137, 153, 637, 755
HaeIII GGCC 2 cut(s) 721, 726
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 77, 143, 178, 460, 763
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HindIII AAGCTT 1 cut(s) 649
HinfI GANTC 3 cut(s) 82, 361, 406
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 596, 614
Hpy188I TCNGA 5 cut(s) 87, 193, 318, 424, 748
Hpy188III TCNNGA 1 cut(s) 551
Hpy8I GTNNAC 2 cut(s) 596, 614
Hpy99I CGWCG 1 cut(s) 771
HpyAV CCTTC 2 cut(s) 331, 481
HpyCH4III ACNGT 1 cut(s) 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 466, 578, 760
HpyF3I CTNAG 4 cut(s) 78, 94, 273, 423
Hsp92II CATG 5 cut(s) 77, 143, 178, 460, 763
HspAI GCGC 1 cut(s) 757
Kzo9I GATC 2 cut(s) 142, 259
LmnI GCTCC 4 cut(s) 226, 269, 447, 664
Lsp1109I GCAGC 6 cut(s) 17, 39, 82, 139, 148, 648
LweI GCATC 1 cut(s) 672
MaeI CTAG 3 cut(s) 461, 536, 582
MaeIII GTNAC 1 cut(s) 530
MalI GATC 2 cut(s) 144, 261
MboI GATC 2 cut(s) 142, 259
MboII GAAGA 3 cut(s) 68, 221, 254
MfeI CAATTG 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 12, 231
MluCI AATT 3 cut(s) 165, 248, 470
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 1 cut(s) 30
Mph1103I ATGCAT 2 cut(s) 458, 687
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 445, 723
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 2 cut(s) 445, 723
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 4 cut(s) 27, 466, 578, 760
NdeI CATATG 1 cut(s) 687
NdeII GATC 2 cut(s) 142, 259
NlaIII CATG 5 cut(s) 77, 143, 178, 460, 763
NmeAIII GCCGAG 1 cut(s) 229
NsiI ATGCAT 2 cut(s) 458, 687
NspI RCATGY 3 cut(s) 77, 460, 763
OliI CACNNNNGTG 1 cut(s) 353
PaeI GCATGC 3 cut(s) 77, 460, 763
PaqCI CACCTGC 1 cut(s) 599
PfeI GAWTC 2 cut(s) 82, 406
PkrI GCNGC 7 cut(s) 29, 32, 72, 138, 154, 638, 756
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 443, 721
PspGI CCWGG 2 cut(s) 443, 721
PspPI GGNCC 5 cut(s) 121, 373, 707, 719, 725
PspPPI RGGWCCY 1 cut(s) 373
PstI CTGCAG 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 7 cut(s) 28, 31, 71, 137, 153, 637, 755
Sau3AI GATC 2 cut(s) 142, 259
Sau96I GGNCC 5 cut(s) 121, 373, 707, 719, 725
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 445, 723
SduI GDGCHC 2 cut(s) 12, 231
SfaNI GCATC 1 cut(s) 672
SfcI CTRYAG 1 cut(s) 215
SinI GGWCC 3 cut(s) 121, 373, 707
SmiMI CAYNNNNRTG 1 cut(s) 353
SphI GCATGC 3 cut(s) 77, 460, 763
Sse9I AATT 3 cut(s) 165, 248, 470
SsiI CCGC 1 cut(s) 755
SspI AATATT 1 cut(s) 496
SspMI CTAG 3 cut(s) 461, 536, 582
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 443, 721
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 600
TaqI TCGA 1 cut(s) 221
TaqII GACCGA 2 cut(s) 138, 724
TasI AATT 3 cut(s) 165, 248, 470
TauI GCSGC 1 cut(s) 757
TfiI GAWTC 2 cut(s) 82, 406
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseI GCWGC 6 cut(s) 27, 30, 70, 136, 152, 636
TspDTI ATGAA 3 cut(s) 158, 191, 494
TspGWI ACGGA 1 cut(s) 651
VpaK11BI GGWCC 3 cut(s) 121, 373, 707
XagI CCTNNNNNAGG 1 cut(s) 702
XapI RAATTY 1 cut(s) 165
XceI RCATGY 3 cut(s) 77, 460, 763
XmiI GTMKAC 1 cut(s) 595
XspI CTAG 3 cut(s) 461, 536, 582
Zsp2I ATGCAT 2 cut(s) 458, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.