Rroxscaffold_2G00102250

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003143
Physical Location & Seq
Forward (+)
24301895 .. 24308723
6829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00102250.1

Sequence Viewer

Length: 771 bp
ATGGTAGAGCCCCCTCCTGTGCCTCGGCAGCTGCCCCATCCTGCAATGTTGGAGGAAGAACTTGAAATGCAGCATGCTGAGATTCAGAGGCTCTTAGCTGATAACCGGAGGCTGGTTGAGGACCGAATGGCATTGCAGCATGATCTCGGTGCTGCCAAGGAGGAAATTCATCGCATGAATGTTGCCTTTTCTGATATTCGTGCCGAGGAAGAACTGCAGTCGAGGGAGCTCTTTGAGAAAGCAAGAAAATTGGAAGCTGATCTTCGGGGAGCTGAGCAGTTTAAAAATGAGGCTAAACAACTACGAGGTGAAGTTCAGAAGCTGAATGGTGTTAGGAAGGAACTTTCCACACAAGTGCAGACTCTCACACAGGACCTTGTAAAGTTGCAAGCTGATAATCAACAGATTCCTTTGCTAAAGTCTGAGATTGATGGTCTGCACCAGGAGCTTATGCATGCTAGAAGTGCAATTGATTATGAAAAGAAGGCAAATATTGAGCTTTTGGAACAGAGACAGGCAATGGAGAGTAACCTAGTTTCTATGGCTCGTGAAGTTGAGAAACTTCGTGCAGAGCTTGCTAGAGTTGATGGTAGACCGTGGGGTGCAGGTGGACAATATGGGATGAGTTTCAGCAGCCCAGATGGAAGCTTTCCTGCTCCGTATGGAGATGGATATGGGATGCATATGGGTATGCCTGATAAAGGACCGATGTATGGGCCAGGCCCAACTTTGTGGGGAGGTTCGGAAAAGCCGCGCATGCCTCGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

29.01

Weight (kDa)

5.62

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccI GTMKAC 1 cut(s) 592
AccII CGCG 1 cut(s) 754
AciI CCGC 1 cut(s) 752
AcsI RAATTY 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 112, 701, 713
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 2 cut(s) 441, 718
AleI CACNNNNGTG 1 cut(s) 353
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 505
AlwNI CAGNNNCTG 1 cut(s) 322
AoxI GGCC 2 cut(s) 716, 721
ApeKI GCWGC 6 cut(s) 28, 31, 70, 136, 152, 633
ApoI RAATTY 1 cut(s) 165
AspLEI GCGC 1 cut(s) 756
AspS9I GGNCC 5 cut(s) 121, 373, 704, 716, 722
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 3 cut(s) 121, 373, 704
BanII GRGCYC 2 cut(s) 12, 231
BauI CACGAG 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 6 cut(s) 18, 40, 82, 139, 148, 645
BccI CCATC 5 cut(s) 45, 425, 581, 635, 662
BciT130I CCWGG 2 cut(s) 443, 720
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 3 cut(s) 459, 533, 579
BfmI CTRYAG 1 cut(s) 215
BfuAI ACCTGC 1 cut(s) 596
BisI GCNGC 7 cut(s) 29, 32, 71, 137, 153, 634, 752
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 7 cut(s) 30, 33, 72, 138, 154, 635, 753
Bme1390I CCNGG 2 cut(s) 443, 720
Bme18I GGWCC 3 cut(s) 121, 373, 704
BmgT120I GGNCC 5 cut(s) 121, 373, 704, 716, 722
BmrFI CCNGG 2 cut(s) 443, 720
BmsI GCATC 1 cut(s) 669
Bpu1102I GCTNAGC 1 cut(s) 273
BsaJI CCNNGG 4 cut(s) 23, 156, 204, 596
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 701, 713
Bse3DI GCAATG 3 cut(s) 51, 131, 525
BseBI CCWGG 2 cut(s) 443, 720
BseDI CCNNGG 4 cut(s) 23, 156, 204, 596
BseGI GGATG 3 cut(s) 37, 627, 684
BseLI CCNNNNNNNGG 3 cut(s) 112, 701, 713
BseMI GCAATG 3 cut(s) 51, 131, 525
BseMII CTCAG 3 cut(s) 69, 264, 414
BseXI GCAGC 6 cut(s) 18, 40, 82, 139, 148, 645
BsgI GTGCAG 4 cut(s) 377, 422, 588, 624
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 2 cut(s) 718, 723
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 106
BslI CCNNNNNNNGG 3 cut(s) 112, 701, 713
BsmAI GTCTC 1 cut(s) 505
BsnI GGCC 2 cut(s) 718, 723
Bsp1286I GDGCHC 2 cut(s) 12, 231
Bsp143I GATC 2 cut(s) 142, 259
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 1 cut(s) 752
BspANI GGCC 2 cut(s) 718, 723
BspCNI CTCAG 3 cut(s) 70, 265, 415
BspFNI CGCG 1 cut(s) 754
BspMAI CTGCAG 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 596
BsrDI GCAATG 3 cut(s) 51, 131, 525
BssECI CCNNGG 4 cut(s) 23, 156, 204, 596
BssMI GATC 2 cut(s) 142, 259
BssSI CACGAG 1 cut(s) 546
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 2 cut(s) 443, 720
Bst4CI ACNGT 1 cut(s) 597
BstAPI GCANNNNNTGC 1 cut(s) 575
BstC8I GCNNGC 5 cut(s) 75, 390, 456, 576, 758
BstDEI CTNAG 4 cut(s) 78, 94, 273, 423
BstDSI CCRYGG 1 cut(s) 596
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 3 cut(s) 37, 627, 684
BstFNI CGCG 1 cut(s) 754
BstHHI GCGC 1 cut(s) 756
BstKTI GATC 2 cut(s) 145, 262
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 2 cut(s) 142, 259
BstMWI GCNNNNNNNGC 5 cut(s) 28, 445, 464, 575, 757
BstNI CCWGG 2 cut(s) 443, 720
BstNSI RCATGY 3 cut(s) 77, 458, 760
BstSCI CCNGG 2 cut(s) 441, 718
BstSFI CTRYAG 1 cut(s) 215
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 6 cut(s) 18, 40, 82, 139, 148, 645
BstXI CCANNNNNNTGG 1 cut(s) 732
BsuRI GGCC 2 cut(s) 718, 723
BtgI CCRYGG 1 cut(s) 596
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 3 cut(s) 37, 627, 684
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 5 cut(s) 75, 390, 456, 576, 758
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 1 cut(s) 756
Cfr13I GGNCC 5 cut(s) 121, 373, 704, 716, 722
CviAII CATG 5 cut(s) 74, 140, 175, 455, 757
DdeI CTNAG 4 cut(s) 78, 94, 273, 423
DpnI GATC 2 cut(s) 144, 261
DpnII GATC 2 cut(s) 142, 259
DraI TTTAAA 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 2 cut(s) 12, 231
Eco47I GGWCC 3 cut(s) 121, 373, 704
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 2 cut(s) 441, 718
EcoT14I CCWWGG 1 cut(s) 156
EcoT22I ATGCAT 2 cut(s) 456, 684
EcoT38I GRGCYC 2 cut(s) 12, 231
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 5 cut(s) 77, 143, 178, 458, 760
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FatI CATG 5 cut(s) 73, 139, 174, 454, 756
FauNDI CATATG 1 cut(s) 684
FblI GTMKAC 1 cut(s) 592
Fnu4HI GCNGC 7 cut(s) 29, 32, 71, 137, 153, 634, 752
FokI GGATG 3 cut(s) 24, 634, 691
FriOI GRGCYC 2 cut(s) 12, 231
Fsp4HI GCNGC 7 cut(s) 29, 32, 71, 137, 153, 634, 752
FspBI CTAG 3 cut(s) 459, 533, 579
GlaI GCGC 1 cut(s) 755
GluI GCNGC 7 cut(s) 29, 32, 71, 137, 153, 634, 752
HaeIII GGCC 2 cut(s) 718, 723
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 756
Hin1II CATG 5 cut(s) 77, 143, 178, 458, 760
Hin6I GCGC 1 cut(s) 754
HinP1I GCGC 1 cut(s) 754
HindIII AAGCTT 1 cut(s) 646
HinfI GANTC 3 cut(s) 82, 361, 406
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 593, 611
Hpy188I TCNGA 5 cut(s) 87, 193, 318, 424, 745
Hpy188III TCNNGA 1 cut(s) 548
Hpy8I GTNNAC 2 cut(s) 593, 611
Hpy99I CGWCG 1 cut(s) 768
HpyAV CCTTC 2 cut(s) 331, 478
HpyCH4III ACNGT 1 cut(s) 597
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 445, 464, 575, 757
HpyF3I CTNAG 4 cut(s) 78, 94, 273, 423
Hsp92II CATG 5 cut(s) 77, 143, 178, 458, 760
HspAI GCGC 1 cut(s) 754
Kzo9I GATC 2 cut(s) 142, 259
LmnI GCTCC 4 cut(s) 226, 269, 445, 661
Lsp1109I GCAGC 6 cut(s) 18, 40, 82, 139, 148, 645
LweI GCATC 1 cut(s) 669
MaeI CTAG 3 cut(s) 459, 533, 579
MaeIII GTNAC 1 cut(s) 527
MalI GATC 2 cut(s) 144, 261
MboI GATC 2 cut(s) 142, 259
MboII GAAGA 3 cut(s) 68, 221, 254
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 2 cut(s) 12, 231
MluCI AATT 3 cut(s) 165, 248, 468
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 1 cut(s) 30
Mph1103I ATGCAT 2 cut(s) 456, 684
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 443, 720
MunI CAATTG 1 cut(s) 468
MvaI CCWGG 2 cut(s) 443, 720
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 5 cut(s) 28, 445, 464, 575, 757
NdeI CATATG 1 cut(s) 684
NdeII GATC 2 cut(s) 142, 259
NlaIII CATG 5 cut(s) 77, 143, 178, 458, 760
NmeAIII GCCGAG 2 cut(s) 4, 229
NsiI ATGCAT 2 cut(s) 456, 684
NspI RCATGY 3 cut(s) 77, 458, 760
OliI CACNNNNGTG 1 cut(s) 353
PaeI GCATGC 3 cut(s) 77, 458, 760
PaqCI CACCTGC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 82, 406
PkrI GCNGC 7 cut(s) 30, 33, 72, 138, 154, 635, 753
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 441, 718
PspGI CCWGG 2 cut(s) 441, 718
PspPI GGNCC 5 cut(s) 121, 373, 704, 716, 722
PspPPI RGGWCCY 1 cut(s) 373
PstI CTGCAG 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 7 cut(s) 29, 32, 71, 137, 153, 634, 752
Sau3AI GATC 2 cut(s) 142, 259
Sau96I GGNCC 5 cut(s) 121, 373, 704, 716, 722
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 443, 720
SduI GDGCHC 2 cut(s) 12, 231
SfaNI GCATC 1 cut(s) 669
SfcI CTRYAG 1 cut(s) 215
SinI GGWCC 3 cut(s) 121, 373, 704
SmiMI CAYNNNNRTG 1 cut(s) 353
SphI GCATGC 3 cut(s) 77, 458, 760
Sse9I AATT 3 cut(s) 165, 248, 468
SsiI CCGC 1 cut(s) 752
SspI AATATT 1 cut(s) 493
SspMI CTAG 3 cut(s) 459, 533, 579
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 441, 718
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 1 cut(s) 597
TaqI TCGA 1 cut(s) 221
TaqII GACCGA 2 cut(s) 138, 721
TasI AATT 3 cut(s) 165, 248, 468
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 2 cut(s) 82, 406
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseI GCWGC 6 cut(s) 28, 31, 70, 136, 152, 633
TspDTI ATGAA 3 cut(s) 158, 191, 492
TspGWI ACGGA 1 cut(s) 648
VpaK11BI GGWCC 3 cut(s) 121, 373, 704
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 1 cut(s) 165
XceI RCATGY 3 cut(s) 77, 458, 760
XmiI GTMKAC 1 cut(s) 592
XspI CTAG 3 cut(s) 459, 533, 579
Zsp2I ATGCAT 2 cut(s) 456, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.