Rorug02G0376400

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003143
Physical Location & Seq
Forward (+)
48040548 .. 48045582
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0376400.1

Sequence Viewer

Length: 921 bp
ATGGAGAACAAAGTGGATGAGGTTGTTGCTGTTGAGCTCCCTGCTCCTCCTGCTTGGAAGAAGAAGATTTTACCCAAGAAGGGAGTTACACCAAGGAAAAATGATGTCACATTCATTTCTCCAACTGGGGAGGAGATTAACAATAAAAAACAACTGGAGCAGTACCTAAAATCACATCCTGGTAACCCTGCATTGTCGGAGTTTGATTGGAGCACAGGTGAAACCCCTAGGAGATCATCCAGGATCAGTGAAAAGGTCAAGTCGACTCCCCCACCAGAGAATGAGCCTCCAAAGAAAAGAGGCCGGAAATCATCAGGTTCAAAGGACAATAAAGTGGAGACTACTGATGCAGAACATGGTACGAATGACACTGAAATGAAAGATGCAGAGGTAGCTGAGAAGAAAGATGGAGAAGCTGAGAAACAGGTTGAGAATGGAGGAAAAGCACCAGAAGAAACTGACCAAATTAAGACCACAGATACCAAAACTGAAGAAACTATCCCAGAGGATGGTAAGGAGACTCAGACTGATGTTATGGAAACTCAAGGTGGAGAGAAAGATGAAAAGTCAAACAGTGTTGCATCAGAGGAACAACCTGCTGAAGTAGCTTTGGCTACTGAGGTAACTGAAAACAACAAGGAAAAGGTGCCACAAGCTGAGGGAGAGGAAGGAAATGGCTCTACTGACAAGAAACAGGACAACACAACAGCTGCTGTGAACATTGAAGAAAATGGAGCACCAGAGAAAGAGAATCTGAAGGGAATAGCACCATCTGTGGAGGAAGAGATCAAAGGGAAACAAGATGTACCAGAGGTCGATGGGAAAAGCGATGCTCCAGCTGAGGAGAAAGTTAAGACAAAGGATGGGGAGGTAGTTGAAAATGGTAAGGTTGACCAAGTGGTTAAACCTGATGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

33.26

Weight (kDa)

4.71

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 9 - 77 3.3e-14 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 604
AccB1I GGYRCC 1 cut(s) 646
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 1 cut(s) 263
AclWI GGATC 1 cut(s) 251
AcuI CTGAAG 3 cut(s) 510, 621, 776
AfaI GTAC 3 cut(s) 164, 361, 807
AfiI CCNNNNNNNGG 2 cut(s) 80, 509
AgsI TTSAA 3 cut(s) 321, 725, 878
AjnI CCWGG 2 cut(s) 178, 239
AluBI AGCT 7 cut(s) 37, 395, 416, 608, 656, 710, 839
AluI AGCT 7 cut(s) 37, 395, 416, 608, 656, 710, 839
Alw21I GWGCWC 3 cut(s) 39, 215, 739
Alw26I GTCTC 2 cut(s) 332, 512
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 713
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 710, 914
AspA2I CCTAGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 230
AvrII CCTAGG 1 cut(s) 227
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 3 cut(s) 39, 215, 739
BbvCI CCTCAGC 2 cut(s) 657, 840
BbvI GCAGC 2 cut(s) 697, 901
BccI CCATC 5 cut(s) 401, 503, 778, 812, 857
BciT130I CCWGG 2 cut(s) 180, 241
BcoDI GTCTC 2 cut(s) 332, 512
BfaI CTAG 1 cut(s) 228
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 2 cut(s) 711, 915
BlnI CCTAGG 1 cut(s) 227
BlsI GCNGC 2 cut(s) 712, 916
Bme1390I CCNGG 2 cut(s) 180, 241
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 2 cut(s) 180, 241
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 5 cut(s) 337, 373, 590, 820, 901
BmuI ACTGGG 1 cut(s) 135
BpmI CTGGAG 2 cut(s) 176, 819
Bpu10I CCTNAGC 2 cut(s) 657, 840
BpuEI CTTGAG 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 92, 227
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 509
Bse1I ACTGG 2 cut(s) 130, 159
BseBI CCWGG 2 cut(s) 180, 241
BseDI CCNNGG 2 cut(s) 92, 227
BseGI GGATG 5 cut(s) 22, 175, 236, 514, 868
BseLI CCNNNNNNNGG 2 cut(s) 80, 509
BseMII CTCAG 6 cut(s) 387, 408, 536, 609, 648, 831
BseNI ACTGG 2 cut(s) 130, 159
BseRI GAGGAG 3 cut(s) 36, 146, 857
BseXI GCAGC 2 cut(s) 697, 901
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 646
BsiHKAI GWGCWC 3 cut(s) 39, 215, 739
BsiSI CCGG 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 80, 509
BsmAI GTCTC 2 cut(s) 332, 512
BsnI GGCC 1 cut(s) 303
Bsp1286I GDGCHC 3 cut(s) 39, 215, 739
Bsp143I GATC 3 cut(s) 233, 243, 786
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 6 cut(s) 388, 409, 535, 610, 649, 832
BspLI GGNNCC 1 cut(s) 648
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 646
BsrI ACTGG 2 cut(s) 130, 159
BssECI CCNNGG 2 cut(s) 92, 227
BssMI GATC 3 cut(s) 233, 243, 786
BssT1I CCWWGG 2 cut(s) 92, 227
Bst2UI CCWGG 2 cut(s) 180, 241
Bst4CI ACNGT 1 cut(s) 575
Bst6I CTCTTC 1 cut(s) 777
BstDEI CTNAG 6 cut(s) 396, 417, 522, 618, 657, 840
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 5 cut(s) 22, 175, 236, 514, 868
BstKTI GATC 3 cut(s) 236, 246, 789
BstMAI GTCTC 2 cut(s) 332, 512
BstMBI GATC 3 cut(s) 233, 243, 786
BstMWI GCNNNNNNNGC 3 cut(s) 50, 392, 605
BstNI CCWGG 2 cut(s) 180, 241
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 178, 239
BstV1I GCAGC 2 cut(s) 697, 901
BsuRI GGCC 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 5 cut(s) 22, 175, 236, 514, 868
BtsIMutI CAGTG 3 cut(s) 253, 369, 580
BveI ACCTGC 1 cut(s) 604
CaiI CAGNNNCTG 1 cut(s) 713
Csp6I GTAC 3 cut(s) 163, 360, 806
CviAII CATG 1 cut(s) 356
CviQI GTAC 3 cut(s) 163, 360, 806
DdeI CTNAG 6 cut(s) 396, 417, 522, 618, 657, 840
DpnI GATC 3 cut(s) 235, 245, 788
DpnII GATC 3 cut(s) 233, 243, 786
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 92, 227
Eco24I GRGCYC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 3 cut(s) 510, 621, 776
Eco91I GGTNACC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 178, 239
EcoT14I CCWWGG 2 cut(s) 92, 227
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 92, 227
FaeI CATG 1 cut(s) 359
FaiI YATR 3 cut(s) 357, 536, 919
FatI CATG 1 cut(s) 355
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 711, 915
FokI GGATG 5 cut(s) 29, 162, 223, 521, 875
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 711, 915
FspBI CTAG 1 cut(s) 228
GluI GCNGC 2 cut(s) 711, 915
GsuI CTGGAG 2 cut(s) 176, 819
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 304
Hin1II CATG 1 cut(s) 359
HincII GTYRAC 2 cut(s) 264, 892
HindII GTYRAC 2 cut(s) 264, 892
HinfI GANTC 3 cut(s) 265, 520, 751
HpaII CCGG 1 cut(s) 304
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 3 cut(s) 264, 718, 892
Hpy188I TCNGA 4 cut(s) 199, 525, 586, 756
Hpy8I GTNNAC 3 cut(s) 264, 718, 892
HpyAV CCTTC 3 cut(s) 73, 662, 751
HpyCH4III ACNGT 1 cut(s) 575
HpyCH4V TGCA 5 cut(s) 191, 350, 386, 581, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 392, 605
HpyF3I CTNAG 6 cut(s) 396, 417, 522, 618, 657, 840
Hsp92II CATG 1 cut(s) 359
Kzo9I GATC 3 cut(s) 233, 243, 786
LmnI GCTCC 6 cut(s) 42, 49, 157, 210, 734, 838
Lsp1109I GCAGC 2 cut(s) 697, 901
LweI GCATC 5 cut(s) 337, 373, 590, 820, 901
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 4 cut(s) 85, 106, 182, 622
MalI GATC 3 cut(s) 235, 245, 788
MboI GATC 3 cut(s) 233, 243, 786
MboII GAAGA 8 cut(s) 70, 73, 76, 412, 464, 503, 737, 794
MhlI GDGCHC 3 cut(s) 39, 215, 739
MluCI AATT 1 cut(s) 465
MlyI GAGTC 2 cut(s) 259, 514
MmeI TCCRAC 2 cut(s) 146, 177
MseI TTAA 4 cut(s) 138, 468, 852, 903
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 710, 839
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 180, 241
MvaI CCWGG 2 cut(s) 180, 241
MwoI GCNNNNNNNGC 3 cut(s) 50, 392, 605
NdeII GATC 3 cut(s) 233, 243, 786
NlaIII CATG 1 cut(s) 359
NlaIV GGNNCC 1 cut(s) 648
NmuCI GTSAC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 751
PflMI CCANNNNNTGG 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 2 cut(s) 712, 916
PleI GAGTC 2 cut(s) 259, 514
PpsI GAGTC 2 cut(s) 259, 514
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 178, 239
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 178, 239
PspN4I GGNNCC 1 cut(s) 648
PstNI CAGNNNCTG 1 cut(s) 713
PvuII CAGCTG 2 cut(s) 710, 839
RsaI GTAC 3 cut(s) 164, 361, 807
RsaNI GTAC 3 cut(s) 163, 360, 806
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 39
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 4 cut(s) 138, 468, 852, 903
SatI GCNGC 2 cut(s) 711, 915
Sau3AI GATC 3 cut(s) 233, 243, 786
SchI GAGTC 2 cut(s) 259, 514
ScrFI CCNGG 2 cut(s) 180, 241
SduI GDGCHC 3 cut(s) 39, 215, 739
SfaNI GCATC 5 cut(s) 337, 373, 590, 820, 901
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 1 cut(s) 465
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 178, 239
StyI CCWWGG 2 cut(s) 92, 227
TaaI ACNGT 1 cut(s) 575
TaqI TCGA 2 cut(s) 263, 816
TasI AATT 1 cut(s) 465
TfiI GAWTC 1 cut(s) 751
Tru1I TTAA 4 cut(s) 138, 468, 852, 903
Tru9I TTAA 4 cut(s) 138, 468, 852, 903
TscAI CASTG 3 cut(s) 253, 376, 580
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 710, 914
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 3 cut(s) 103, 392, 576
TspRI CASTG 3 cut(s) 253, 376, 580
Van91I CCANNNNNTGG 1 cut(s) 509
XmaJI CCTAGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.