RLG00000019994

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003143
Physical Location & Seq
Reverse (-)
59779938 .. 59786114
6177 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019994

Sequence Viewer

Length: 966 bp
ATGCCAGAATATAGCCGTGCCGATGAAATAAAAAGGGCCCTCAAACGCATAGCTTTTCTTCAAAATTTCCATCAAGAACAAGGGATAACATCTCTGATGGCAGGGAGGCACCGCATTCCGCGTGAACCCATGATTGACCGGCGTGGCTACCCTCTTGAAGGACCTTATGGTAGAGCCCCTCCTGTGCCTCGGCAGCTGCCCCATCCTGCAATGTTGGAGGAAGAACTTGAAATGCAGCATGCTGAAATCCGGAGGCTCTTAGCTGATAACCGGAGGCTGGTTGAGGACCGAATGGCATTGCAGCATGATCTTGGTGCTGCCAAGGAGGAAATTCATCGCATGAATGTTGCCTTTTCTGATATTCGCTCCGAGGAAGAACTGCAGTCGAGGGAGCTCTTCGAGAAAGCAAGAAAATTGGAAGCTGATCTTAGGGGAGCTGAGCCGTTTAAAAATGAGGCTAAACAACTACGCAGTGAAGTTCAGAAGCTGAATGGTGTTAGGAAGGAACTTTCCACACAAGTGCAGACTCTCACACAAGACCTTGTAAAGTTGCAAGCTGATAATCAACAGATTCCTTTGCTAAAGTCTGAGATTGATGGTCTGCACCAGGAGCTTATGCATGCTAGAAGTGCAATTGATTATGAAAAGAAGGCAAATATTGAGCTTATGGAACAGAGACAGGCAATGGAGAATAACCTAGTTTCTATGGCTCGCGAAGTTGAGAAACTTCGTGCAGAACTTGCTAGAGTTGATGGTAGACCGTGGGGTGCAGGTGGACAATATGGGATGAGTTTCAGCAGCCCAGATGGAAGCTTTCCTGCTCCGTATGGAGATGGATATGGGATGCATATGGGTATGCCTGATAAAGGACCGATGTATGGGCCAGGCCCAACTTTGTGGGGAGGTTGTTCGTCGTCTGGAGATTATGGCCAAGAATTTATAGATGGAAGGCCTGTTGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

36.44

Weight (kDa)

5.82

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 761
AccB1I GGYRCC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 757
AccII CGCG 2 cut(s) 121, 714
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 2 cut(s) 112, 119
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 3 cut(s) 64, 330, 935
AfiI CCNNNNNNNGG 4 cut(s) 158, 277, 866, 878
AgsI TTSAA 3 cut(s) 62, 158, 230
AjnI CCWGG 2 cut(s) 606, 883
AleI CACNNNNGTG 1 cut(s) 518
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 670
AlwNI CAGNNNCTG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 5 cut(s) 36, 881, 886, 928, 950
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 6 cut(s) 193, 196, 235, 301, 317, 798
ApoI RAATTY 3 cut(s) 64, 330, 935
AspS9I GGNCC 7 cut(s) 36, 37, 161, 286, 869, 881, 887
AvaII GGWCC 3 cut(s) 161, 286, 869
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 930
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 3 cut(s) 40, 178, 396
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 6 cut(s) 183, 205, 247, 304, 313, 810
BccI CCATC 8 cut(s) 78, 91, 210, 590, 746, 800, 827, 938
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 608, 885
BcoDI GTCTC 1 cut(s) 670
BfaI CTAG 3 cut(s) 624, 698, 744
BfmI CTRYAG 1 cut(s) 380
BfuAI ACCTGC 1 cut(s) 761
BisI GCNGC 6 cut(s) 194, 197, 236, 302, 318, 799
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 6 cut(s) 195, 198, 237, 303, 319, 800
Bme1390I CCNGG 2 cut(s) 608, 885
Bme18I GGWCC 3 cut(s) 161, 286, 869
BmgT120I GGNCC 7 cut(s) 36, 37, 161, 286, 869, 881, 887
BmiI GGNNCC 2 cut(s) 38, 110
BmrFI CCNGG 2 cut(s) 608, 885
BmsI GCATC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 438
BsaJI CCNNGG 4 cut(s) 188, 321, 369, 761
BsaWI WCCGGW 2 cut(s) 249, 270
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 277, 866, 878
Bse118I RCCGGY 1 cut(s) 138
Bse3DI GCAATG 3 cut(s) 216, 296, 690
BseAI TCCGGA 1 cut(s) 249
BseBI CCWGG 2 cut(s) 608, 885
BseDI CCNNGG 4 cut(s) 188, 321, 369, 761
BseGI GGATG 3 cut(s) 202, 792, 849
BseLI CCNNNNNNNGG 4 cut(s) 158, 277, 866, 878
BseMI GCAATG 3 cut(s) 216, 296, 690
BseMII CTCAG 2 cut(s) 429, 579
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 6 cut(s) 183, 205, 247, 304, 313, 810
BsgI GTGCAG 4 cut(s) 542, 587, 753, 789
Bsh1236I CGCG 2 cut(s) 121, 714
BshFI GGCC 5 cut(s) 38, 883, 888, 930, 952
BshNI GGYRCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 3 cut(s) 139, 250, 271
BslI CCNNNNNNNGG 4 cut(s) 158, 277, 866, 878
BsmAI GTCTC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 5 cut(s) 38, 883, 888, 930, 952
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 3 cut(s) 40, 178, 396
Bsp13I TCCGGA 1 cut(s) 249
Bsp143I GATC 2 cut(s) 307, 424
Bsp1720I GCTNAGC 1 cut(s) 438
Bsp68I TCGCGA 1 cut(s) 714
BspACI CCGC 2 cut(s) 112, 119
BspANI GGCC 5 cut(s) 38, 883, 888, 930, 952
BspCNI CTCAG 2 cut(s) 430, 580
BspEI TCCGGA 1 cut(s) 249
BspFNI CGCG 2 cut(s) 121, 714
BspLI GGNNCC 2 cut(s) 38, 110
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 761
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 108
BsrDI GCAATG 3 cut(s) 216, 296, 690
BsrFI RCCGGY 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 4 cut(s) 188, 321, 369, 761
BssMI GATC 2 cut(s) 307, 424
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 608, 885
Bst4CI ACNGT 1 cut(s) 762
Bst6I CTCTTC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 740
BstC8I GCNNGC 4 cut(s) 240, 555, 621, 712
BstDEI CTNAG 4 cut(s) 259, 428, 438, 588
BstDSI CCRYGG 1 cut(s) 761
BstENI CCTNNNNNAGG 2 cut(s) 156, 864
BstF5I GGATG 3 cut(s) 202, 792, 849
BstFNI CGCG 2 cut(s) 121, 714
BstKTI GATC 2 cut(s) 310, 427
BstMAI GTCTC 1 cut(s) 670
BstMBI GATC 2 cut(s) 307, 424
BstMWI GCNNNNNNNGC 4 cut(s) 193, 610, 629, 740
BstNI CCWGG 2 cut(s) 608, 885
BstNSI RCATGY 2 cut(s) 242, 623
BstSCI CCNGG 2 cut(s) 606, 883
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 2 cut(s) 121, 714
BstV1I GCAGC 6 cut(s) 183, 205, 247, 304, 313, 810
BstXI CCANNNNNNTGG 1 cut(s) 897
BsuRI GGCC 5 cut(s) 38, 883, 888, 930, 952
BtgI CCRYGG 1 cut(s) 761
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 3 cut(s) 202, 792, 849
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 478
BtuMI TCGCGA 1 cut(s) 714
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 4 cut(s) 240, 555, 621, 712
CaiI CAGNNNCTG 1 cut(s) 487
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 7 cut(s) 36, 37, 161, 286, 869, 881, 887
CviAII CATG 5 cut(s) 130, 239, 305, 340, 620
DdeI CTNAG 4 cut(s) 259, 428, 438, 588
DpnI GATC 2 cut(s) 309, 426
DpnII GATC 2 cut(s) 307, 424
DraI TTTAAA 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 321
Eco147I AGGCCT 1 cut(s) 952
Eco24I GRGCYC 3 cut(s) 40, 178, 396
Eco47I GGWCC 3 cut(s) 161, 286, 869
Eco53kI GAGCTC 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 2 cut(s) 156, 864
EcoO109I RGGNCCY 3 cut(s) 36, 37, 161
EcoRII CCWGG 2 cut(s) 606, 883
EcoT14I CCWWGG 1 cut(s) 321
EcoT22I ATGCAT 2 cut(s) 621, 849
EcoT38I GRGCYC 3 cut(s) 40, 178, 396
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 5 cut(s) 133, 242, 308, 343, 623
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 5 cut(s) 129, 238, 304, 339, 619
FauNDI CATATG 1 cut(s) 849
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 6 cut(s) 194, 197, 236, 302, 318, 799
FokI GGATG 3 cut(s) 189, 799, 856
FriOI GRGCYC 3 cut(s) 40, 178, 396
Fsp4HI GCNGC 6 cut(s) 194, 197, 236, 302, 318, 799
FspBI CTAG 3 cut(s) 624, 698, 744
GluI GCNGC 6 cut(s) 194, 197, 236, 302, 318, 799
GsuI CTGGAG 1 cut(s) 939
HaeIII GGCC 5 cut(s) 38, 883, 888, 930, 952
HapII CCGG 3 cut(s) 139, 250, 271
Hin1II CATG 5 cut(s) 133, 242, 308, 343, 623
HindIII AAGCTT 1 cut(s) 811
HinfI GANTC 2 cut(s) 526, 571
HpaII CCGG 3 cut(s) 139, 250, 271
Hpy166II GTNNAC 3 cut(s) 125, 758, 776
Hpy188I TCNGA 5 cut(s) 96, 358, 370, 483, 589
Hpy188III TCNNGA 6 cut(s) 74, 155, 250, 400, 713, 918
Hpy8I GTNNAC 3 cut(s) 125, 758, 776
Hpy99I CGWCG 1 cut(s) 916
HpyAV CCTTC 4 cut(s) 152, 496, 643, 942
HpyCH4III ACNGT 1 cut(s) 762
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 610, 629, 740
HpyF3I CTNAG 4 cut(s) 259, 428, 438, 588
Hsp92II CATG 5 cut(s) 133, 242, 308, 343, 623
Kpn2I TCCGGA 1 cut(s) 249
Kzo9I GATC 2 cut(s) 307, 424
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 5 cut(s) 371, 391, 434, 610, 826
Lsp1109I GCAGC 6 cut(s) 183, 205, 247, 304, 313, 810
LweI GCATC 1 cut(s) 834
MaeI CTAG 3 cut(s) 624, 698, 744
MalI GATC 2 cut(s) 309, 426
MboI GATC 2 cut(s) 307, 424
MboII GAAGA 4 cut(s) 50, 233, 386, 388
MfeI CAATTG 1 cut(s) 633
MhlI GDGCHC 3 cut(s) 40, 178, 396
MlsI TGGCCA 1 cut(s) 930
MluCI AATT 5 cut(s) 64, 330, 413, 633, 935
MluNI TGGCCA 1 cut(s) 930
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 930
Mph1103I ATGCAT 2 cut(s) 621, 849
MroI TCCGGA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 930
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 518
Msp20I TGGCCA 1 cut(s) 930
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 3 cut(s) 139, 250, 271
MspR9I CCNGG 2 cut(s) 608, 885
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 2 cut(s) 608, 885
MvnI CGCG 2 cut(s) 121, 714
MwoI GCNNNNNNNGC 4 cut(s) 193, 610, 629, 740
NdeI CATATG 1 cut(s) 849
NdeII GATC 2 cut(s) 307, 424
NlaIII CATG 5 cut(s) 133, 242, 308, 343, 623
NlaIV GGNNCC 2 cut(s) 38, 110
NmeAIII GCCGAG 1 cut(s) 169
NruI TCGCGA 1 cut(s) 714
NsiI ATGCAT 2 cut(s) 621, 849
NspI RCATGY 2 cut(s) 242, 623
OliI CACNNNNGTG 1 cut(s) 518
PaeI GCATGC 2 cut(s) 242, 623
PaqCI CACCTGC 1 cut(s) 761
PceI AGGCCT 1 cut(s) 952
PciSI GCTCTTC 1 cut(s) 401
PctI GAATGC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 571
PkrI GCNGC 6 cut(s) 195, 198, 237, 303, 319, 800
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PpuMI RGGWCCY 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 606, 883
PspGI CCWGG 2 cut(s) 606, 883
PspN4I GGNNCC 2 cut(s) 38, 110
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 7 cut(s) 36, 37, 161, 286, 869, 881, 887
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 1 cut(s) 384
PstNI CAGNNNCTG 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 196
RruI TCGCGA 1 cut(s) 714
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 6 cut(s) 194, 197, 236, 302, 318, 799
Sau3AI GATC 2 cut(s) 307, 424
Sau96I GGNCC 7 cut(s) 36, 37, 161, 286, 869, 881, 887
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 2 cut(s) 608, 885
SduI GDGCHC 3 cut(s) 40, 178, 396
SfaNI GCATC 1 cut(s) 834
SfcI CTRYAG 1 cut(s) 380
SinI GGWCC 3 cut(s) 161, 286, 869
SmiMI CAYNNNNRTG 1 cut(s) 518
SphI GCATGC 2 cut(s) 242, 623
Sse9I AATT 5 cut(s) 64, 330, 413, 633, 935
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 2 cut(s) 112, 119
SspI AATATT 1 cut(s) 658
SspMI CTAG 3 cut(s) 624, 698, 744
SstI GAGCTC 1 cut(s) 396
StuI AGGCCT 1 cut(s) 952
StyD4I CCNGG 2 cut(s) 606, 883
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 762
TaqI TCGA 2 cut(s) 386, 399
TaqII GACCGA 2 cut(s) 303, 886
TasI AATT 5 cut(s) 64, 330, 413, 633, 935
TfiI GAWTC 1 cut(s) 571
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 478
TseI GCWGC 6 cut(s) 193, 196, 235, 301, 317, 798
TspDTI ATGAA 4 cut(s) 39, 323, 356, 657
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 3 cut(s) 161, 286, 869
XagI CCTNNNNNAGG 2 cut(s) 156, 864
XapI RAATTY 3 cut(s) 64, 330, 935
XceI RCATGY 2 cut(s) 242, 623
XmiI GTMKAC 1 cut(s) 757
XspI CTAG 3 cut(s) 624, 698, 744
Zsp2I ATGCAT 2 cut(s) 621, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.