Rmu_sc0003940.1_g000005

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003940.1
OG0003143
Physical Location & Seq
Forward (+)
39236 .. 42767
3532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003940.1_g000005.1.cds

Sequence Viewer

Length: 561 bp
atgttcctcatgtgcatgcgagcgcgtctgagactagttcaggtaaatgtctcgcttccgatgttccccgaggtcacaccagcgagacctttgcatggccggagtggtctccatgtcatggctgattatgctcgaacaacggagctcgatttcaaaatatcggagctcgctttctctcctcttgccttctctacgaatcgcagaggttgggcttcaatcggtattcctttgctaaagtctgagattgatggtctgcaccaggagcttatgcatgctagaagtgcaattgattatgaaaagaaggcaaatattgagcttatggaacagaaacaagcaatggagaataacctagtttctatggctcgtgaagttgagaaacttcgtgcagaacttgctagagttgatggtagaccgtggggtgcaggtagtctcctgtttacgttttcaagaaatgcagatcttatcgcatcaggggtttggaagaacatgggtaaagttgggaactacatgaaagaaagtgtttctgatactttgtacccctacagagggcgaccaaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.06

Weight (kDa)

9.58

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 118
AccI GTMKAC 1 cut(s) 409
AccII CGCG 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 97
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 4 cut(s) 95, 118, 545, 546
AgsI TTSAA 3 cut(s) 154, 216, 447
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 4 cut(s) 145, 166, 265, 316
AluI AGCT 4 cut(s) 145, 166, 265, 316
Alw21I GWGCWC 2 cut(s) 147, 168
Alw26I GTCTC 5 cut(s) 25, 55, 79, 113, 434
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 68
BanII GRGCYC 2 cut(s) 147, 168
BauI CACGAG 1 cut(s) 363
Bbv12I GWGCWC 2 cut(s) 147, 168
BccI CCATC 2 cut(s) 242, 398
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 1 cut(s) 260
BcoDI GTCTC 5 cut(s) 25, 55, 79, 113, 434
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 4 cut(s) 35, 276, 350, 396
BfmI CTRYAG 1 cut(s) 541
BfuAI ACCTGC 1 cut(s) 413
BglII AGATCT 1 cut(s) 457
Bme1390I CCNGG 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 68
BmrFI CCNGG 1 cut(s) 260
BmsI GCATC 1 cut(s) 476
BsaI GGTCTC 2 cut(s) 79, 113
BsaJI CCNNGG 2 cut(s) 69, 413
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 118, 545, 546
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 2 cut(s) 69, 413
BseLI CCNNNNNNNGG 4 cut(s) 95, 118, 545, 546
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 20, 231
BseRI GAGGAG 1 cut(s) 168
BsgI GTGCAG 3 cut(s) 239, 405, 441
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 147, 168
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 100
BslI CCNNNNNNNGG 4 cut(s) 95, 118, 545, 546
BsmAI GTCTC 5 cut(s) 25, 55, 79, 113, 434
BsnI GGCC 1 cut(s) 99
Bso31I GGTCTC 2 cut(s) 79, 113
BsoBI CYCGRG 1 cut(s) 68
Bsp1286I GDGCHC 2 cut(s) 147, 168
Bsp143I GATC 1 cut(s) 457
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 2 cut(s) 21, 232
BspFNI CGCG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 413
BspTNI GGTCTC 2 cut(s) 79, 113
BsrDI GCAATG 1 cut(s) 342
BssECI CCNNGG 2 cut(s) 69, 413
BssMI GATC 1 cut(s) 457
BssSI CACGAG 1 cut(s) 363
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 414
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 4 cut(s) 17, 21, 168, 273
BstDEI CTNAG 2 cut(s) 29, 240
BstDSI CCRYGG 1 cut(s) 413
BstFNI CGCG 1 cut(s) 25
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 5 cut(s) 25, 55, 79, 113, 434
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 4 cut(s) 128, 262, 281, 392
BstNI CCWGG 1 cut(s) 260
BstNSI RCATGY 2 cut(s) 19, 275
BstSCI CCNGG 1 cut(s) 258
BstSFI CTRYAG 1 cut(s) 541
BstUI CGCG 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 413
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 4 cut(s) 17, 21, 168, 273
CfoI GCGC 1 cut(s) 25
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 535
CviAII CATG 8 cut(s) 10, 16, 95, 113, 118, 272, 487, 508
CviJI RGCY 8 cut(s) 99, 122, 145, 166, 212, 265, 316, 362
CviKI_1 RGCY 8 cut(s) 99, 122, 145, 166, 212, 265, 316, 362
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 2 cut(s) 29, 240
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 97
Ecl136II GAGCTC 2 cut(s) 145, 166
Eco24I GRGCYC 2 cut(s) 147, 168
Eco31I GGTCTC 2 cut(s) 79, 113
Eco53kI GAGCTC 2 cut(s) 145, 166
Eco88I CYCGRG 1 cut(s) 68
EcoICRI GAGCTC 2 cut(s) 145, 166
EcoRII CCWGG 1 cut(s) 258
EcoT22I ATGCAT 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 147, 168
FaeI CATG 8 cut(s) 13, 19, 98, 116, 121, 275, 490, 511
FatI CATG 8 cut(s) 9, 15, 94, 112, 117, 271, 486, 507
FblI GTMKAC 1 cut(s) 409
FriOI GRGCYC 2 cut(s) 147, 168
FspBI CTAG 4 cut(s) 35, 276, 350, 396
GlaI GCGC 1 cut(s) 24
HaeIII GGCC 1 cut(s) 99
HapII CCGG 1 cut(s) 100
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 25
Hin1II CATG 8 cut(s) 13, 19, 98, 116, 121, 275, 490, 511
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 1 cut(s) 196
HpaII CCGG 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 410, 438
Hpy188I TCNGA 5 cut(s) 30, 60, 163, 241, 526
Hpy188III TCNNGA 2 cut(s) 365, 447
Hpy8I GTNNAC 2 cut(s) 410, 438
HpyAV CCTTC 2 cut(s) 196, 295
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 8 cut(s) 15, 94, 256, 271, 284, 386, 422, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 262, 281, 392
HpyF3I CTNAG 2 cut(s) 29, 240
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 8 cut(s) 13, 19, 98, 116, 121, 275, 490, 511
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 3 cut(s) 142, 163, 262
LpnPI CCDG 8 cut(s) 26, 93, 113, 245, 272, 408, 446, 456
LweI GCATC 1 cut(s) 476
MaeI CTAG 4 cut(s) 35, 276, 350, 396
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 73
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 1 cut(s) 493
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 2 cut(s) 147, 168
MluCI AATT 1 cut(s) 285
MnlI CCTC 5 cut(s) 17, 64, 189, 197, 539
Mph1103I ATGCAT 1 cut(s) 273
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 260
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 4 cut(s) 128, 262, 281, 392
NdeII GATC 1 cut(s) 457
NlaIII CATG 8 cut(s) 13, 19, 98, 116, 121, 275, 490, 511
NmuCI GTSAC 1 cut(s) 73
NsiI ATGCAT 1 cut(s) 273
NspI RCATGY 2 cut(s) 19, 275
PaeI GCATGC 2 cut(s) 19, 275
PfeI GAWTC 1 cut(s) 196
PflMI CCANNNNNTGG 1 cut(s) 118
Psp124BI GAGCTC 2 cut(s) 147, 168
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 457
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 2 cut(s) 147, 168
Sau3AI GATC 1 cut(s) 457
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 147, 168
SfaNI GCATC 1 cut(s) 476
SfcI CTRYAG 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 14
SpeI ACTAGT 1 cut(s) 34
SphI GCATGC 2 cut(s) 19, 275
Sse9I AATT 1 cut(s) 285
SspI AATATT 1 cut(s) 310
SspMI CTAG 4 cut(s) 35, 276, 350, 396
SstI GAGCTC 2 cut(s) 147, 168
StyD4I CCNGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 1 cut(s) 443
TaqI TCGA 2 cut(s) 133, 147
TasI AATT 1 cut(s) 285
TfiI GAWTC 1 cut(s) 196
TseFI GTSAC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 309, 524
TspGWI ACGGA 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 118
XceI RCATGY 2 cut(s) 19, 275
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 4 cut(s) 35, 276, 350, 396
Zsp2I ATGCAT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.