MD17G1175100.v1.1

FLX-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003143
Physical Location & Seq
Forward (+)
19611380 .. 19617904
6525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1175100.v1.1.491

Sequence Viewer

Length: 804 bp
ATGAATGACCGGCGTGGTTATCCTATTGAAGGACCTTATGGCCGAGGTCCTCCTATGCGGTCTCTTCCCCATCCTGCAATGTTGGAGGAAGAGCTTGAAATGCAACATGCGGAAATTCGGAGACTCTTGGCTGATAACCGGAGGCTGGTTGAGGACCGGATGGTGTTGCAGCATGAGCTTGGTGCTGCCAAGGAAGAAATTCATCGGATGAATGTTGCCTTTTCAGATATTCGTGCCGAAGAAGAAATGAAATCAAGGGAGCTCTATGAGAAAAGTAGGAAATTGGAGGCTGATCTACAGGCAACTGAGCCCTTGAAAAATGAGGCTAAGCAACTTCGCTCTGAAGTTCAGAAGCTGAATACTGTTAGGAAGGAGCTTGCCACACAAGTTCAGACCCTCACGCAGGACCTTGCGAGGTTGCAGTCTGACAATAAGCAGATTCCTATGCTGAGGACTGAGATTGATGGTCTGCATCAGGAGCTTATGTTTTGTCGAAATGCGGTTGATTATGAAAAGAAGGCAAATATTGAGCTTATGGAGCAAAGACAGGCAATGGAGAAGAACCTAGTTTCTATGGCACGTGAAGTTGAGAAACTACGGGCAGAACTTTCTAGGGCTGATGGTAGACCATGGGGTACAGGTGGACAATATGGTATGAATTTCACCAGCCCAGAAGGGGCTTTTCCTGCTGCATATGGAGATGGATATGCGATGCACATGGGTGCTGCTGACAAAGGGCCTATGTATGGCCTGGGTCCAGCTTCATGGGGAGGTTCTGAAAAGCCTCGCATGACTCGTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

30.51

Weight (kDa)

6.36

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 625
AciI CCGC 3 cut(s) 58, 110, 500
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 3 cut(s) 114, 198, 658
AcuI CTGAAG 1 cut(s) 363
AcvI CACGTG 1 cut(s) 581
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 5 cut(s) 29, 145, 403, 676, 746
AgsI TTSAA 3 cut(s) 29, 98, 316
AjnI CCWGG 1 cut(s) 750
AleI CACNNNNGTG 1 cut(s) 720
AluBI AGCT 8 cut(s) 94, 178, 262, 355, 376, 481, 532, 761
AluI AGCT 8 cut(s) 94, 178, 262, 355, 376, 481, 532, 761
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 2 cut(s) 66, 115
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 3 cut(s) 40, 737, 748
ApeKI GCWGC 4 cut(s) 169, 185, 689, 725
ApoI RAATTY 3 cut(s) 114, 198, 658
Asp700I GAANNNNTTC 1 cut(s) 198
AspS9I GGNCC 6 cut(s) 32, 47, 154, 406, 737, 755
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 5 cut(s) 32, 47, 154, 406, 755
BanII GRGCYC 2 cut(s) 264, 312
BbrPI CACGTG 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 4 cut(s) 172, 181, 676, 712
BccI CCATC 5 cut(s) 78, 154, 458, 614, 695
BciT130I CCWGG 1 cut(s) 752
BcoDI GTCTC 2 cut(s) 66, 115
BfaI CTAG 2 cut(s) 566, 612
BfmI CTRYAG 1 cut(s) 296
BisI GCNGC 4 cut(s) 170, 186, 690, 726
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 4 cut(s) 171, 187, 691, 727
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 5 cut(s) 32, 47, 154, 406, 755
BmgT120I GGNCC 6 cut(s) 32, 47, 154, 406, 737, 755
BmiI GGNNCC 1 cut(s) 756
BmrFI CCNGG 1 cut(s) 752
BmsI GCATC 2 cut(s) 481, 702
Bpu10I CCTNAGC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 43, 189, 629, 751
BsaWI WCCGGW 2 cut(s) 138, 156
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 145, 403, 676, 746
Bse118I RCCGGY 1 cut(s) 9
Bse3DI GCAATG 2 cut(s) 84, 558
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 43, 189, 629, 751
BseGI GGATG 3 cut(s) 70, 165, 213
BseLI CCNNNNNNNGG 5 cut(s) 29, 145, 403, 676, 746
BseMI GCAATG 2 cut(s) 84, 558
BseMII CTCAG 3 cut(s) 297, 440, 447
BseXI GCAGC 4 cut(s) 172, 181, 676, 712
BshFI GGCC 3 cut(s) 42, 739, 750
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 10, 139, 157
BslI CCNNNNNNNGG 5 cut(s) 29, 145, 403, 676, 746
BsmAI GTCTC 2 cut(s) 66, 115
BsnI GGCC 3 cut(s) 42, 739, 750
Bso31I GGTCTC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 264, 312
Bsp143I GATC 1 cut(s) 292
Bsp1720I GCTNAGC 1 cut(s) 327
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 3 cut(s) 58, 110, 500
BspANI GGCC 3 cut(s) 42, 739, 750
BspCNI CTCAG 3 cut(s) 298, 441, 448
BspLI GGNNCC 1 cut(s) 756
BspQI GCTCTTC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 84, 558
BsrFI RCCGGY 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 4 cut(s) 43, 189, 629, 751
BssMI GATC 1 cut(s) 292
BssT1I CCWWGG 2 cut(s) 189, 629
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 2 cut(s) 69, 84
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 4 cut(s) 306, 327, 449, 456
BstDSI CCRYGG 1 cut(s) 629
BstENI CCTNNNNNAGG 2 cut(s) 27, 401
BstF5I GGATG 3 cut(s) 70, 165, 213
BstKTI GATC 1 cut(s) 295
BstMAI GTCTC 2 cut(s) 66, 115
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 5 cut(s) 100, 175, 478, 538, 686
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 4 cut(s) 172, 181, 676, 712
BstXI CCANNNNNNTGG 1 cut(s) 765
BsuRI GGCC 3 cut(s) 42, 739, 750
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 725
BtsCI GGATG 3 cut(s) 70, 165, 213
Cac8I GCNNGC 1 cut(s) 378
CaiI CAGNNNCTG 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 6 cut(s) 32, 47, 154, 406, 737, 755
Csp6I GTAC 1 cut(s) 636
CviAII CATG 6 cut(s) 107, 173, 630, 718, 765, 790
CviQI GTAC 1 cut(s) 636
DdeI CTNAG 4 cut(s) 306, 327, 449, 456
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 69, 84
EarI CTCTTC 2 cut(s) 69, 84
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 189, 629
Eco24I GRGCYC 2 cut(s) 264, 312
Eco31I GGTCTC 1 cut(s) 66
Eco47I GGWCC 5 cut(s) 32, 47, 154, 406, 755
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 363
Eco72I CACGTG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 2 cut(s) 27, 401
EcoO109I RGGNCCY 4 cut(s) 32, 47, 406, 737
EcoRII CCWGG 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 189, 629
EcoT38I GRGCYC 2 cut(s) 264, 312
ErhI CCWWGG 2 cut(s) 189, 629
FaeI CATG 6 cut(s) 110, 176, 633, 721, 768, 793
FatI CATG 6 cut(s) 106, 172, 629, 717, 764, 789
FauNDI CATATG 1 cut(s) 694
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 4 cut(s) 170, 186, 690, 726
FokI GGATG 3 cut(s) 57, 172, 220
FriOI GRGCYC 2 cut(s) 264, 312
Fsp4HI GCNGC 4 cut(s) 170, 186, 690, 726
FspBI CTAG 2 cut(s) 566, 612
GluI GCNGC 4 cut(s) 170, 186, 690, 726
HaeIII GGCC 3 cut(s) 42, 739, 750
HapII CCGG 3 cut(s) 10, 139, 157
Hin1II CATG 6 cut(s) 110, 176, 633, 721, 768, 793
HinfI GANTC 3 cut(s) 123, 439, 793
HpaII CCGG 3 cut(s) 10, 139, 157
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 2 cut(s) 626, 644
Hpy188I TCNGA 8 cut(s) 120, 207, 226, 343, 351, 393, 427, 778
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 2 cut(s) 626, 644
Hpy99I CGWCG 1 cut(s) 801
HpyAV CCTTC 4 cut(s) 23, 364, 511, 668
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 7 cut(s) 77, 103, 169, 421, 472, 692, 715
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 175, 478, 538, 686
HpyF3I CTNAG 4 cut(s) 306, 327, 449, 456
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 6 cut(s) 110, 176, 633, 721, 768, 793
Kzo9I GATC 1 cut(s) 292
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 4 cut(s) 259, 373, 478, 538
Lsp1109I GCAGC 4 cut(s) 172, 181, 676, 712
LweI GCATC 2 cut(s) 481, 702
MaeI CTAG 2 cut(s) 566, 612
MaeII ACGT 1 cut(s) 580
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 6 cut(s) 56, 101, 206, 251, 254, 571
MhlI GDGCHC 2 cut(s) 264, 312
MluCI AATT 4 cut(s) 114, 198, 281, 658
MlyI GAGTC 2 cut(s) 117, 787
MmeI TCCRAC 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 720
MspI CCGG 3 cut(s) 10, 139, 157
MspR9I CCNGG 1 cut(s) 752
MvaI CCWGG 1 cut(s) 752
MwoI GCNNNNNNNGC 5 cut(s) 100, 175, 478, 538, 686
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 694
NdeII GATC 1 cut(s) 292
NlaIII CATG 6 cut(s) 110, 176, 633, 721, 768, 793
NlaIV GGNNCC 1 cut(s) 756
NmeAIII GCCGAG 1 cut(s) 68
NspI RCATGY 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 4 cut(s) 171, 187, 691, 727
PleI GAGTC 2 cut(s) 117, 787
PmaCI CACGTG 1 cut(s) 581
PmlI CACGTG 1 cut(s) 581
PpsI GAGTC 2 cut(s) 117, 787
Ppu21I YACGTR 1 cut(s) 581
PpuMI RGGWCCY 3 cut(s) 32, 47, 406
Psp124BI GAGCTC 1 cut(s) 264
Psp5II RGGWCCY 3 cut(s) 32, 47, 406
Psp6I CCWGG 1 cut(s) 750
PspCI CACGTG 1 cut(s) 581
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 6 cut(s) 32, 47, 154, 406, 737, 755
PspPPI RGGWCCY 3 cut(s) 32, 47, 406
PstNI CAGNNNCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
RseI CAYNNNNRTG 1 cut(s) 720
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 84
SatI GCNGC 4 cut(s) 170, 186, 690, 726
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 6 cut(s) 32, 47, 154, 406, 737, 755
SchI GAGTC 2 cut(s) 117, 787
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 2 cut(s) 264, 312
SfaNI GCATC 2 cut(s) 481, 702
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 5 cut(s) 32, 47, 154, 406, 755
SmiMI CAYNNNNRTG 1 cut(s) 720
Sse9I AATT 4 cut(s) 114, 198, 281, 658
SsiI CCGC 3 cut(s) 58, 110, 500
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 566, 612
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 2 cut(s) 189, 629
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 583
TaqI TCGA 1 cut(s) 493
TasI AATT 4 cut(s) 114, 198, 281, 658
TfiI GAWTC 1 cut(s) 439
TseI GCWGC 4 cut(s) 169, 185, 689, 725
TspDTI ATGAA 7 cut(s) 17, 191, 224, 263, 525, 671, 753
VpaK11BI GGWCC 5 cut(s) 32, 47, 154, 406, 755
XagI CCTNNNNNAGG 2 cut(s) 27, 401
XapI RAATTY 3 cut(s) 114, 198, 658
XceI RCATGY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 566, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.